{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:40:08Z","timestamp":1791272408664,"version":"4.1.0"},"reference-count":22,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental and Molecular Pathology"],"published-print":{"date-parts":[[2015,12]]},"DOI":"10.1016\/j.yexmp.2015.09.004","type":"journal-article","created":{"date-parts":[[2015,9,26]],"date-time":"2015-09-26T02:32:53Z","timestamp":1443234773000},"page":"485-491","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":48,"title":["Multi-center evaluation of the novel fully-automated PCR-based Idylla\u2122 BRAF Mutation Test on formalin-fixed paraffin-embedded tissue of malignant melanoma"],"prefix":"10.1016","volume":"99","author":[{"given":"Linea","family":"Melchior","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morten","family":"Grauslund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Bellosillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Montagut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica","family":"Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ester","family":"Morag\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Micalessi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"Frans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veerle","family":"Noten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Bourgain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renske","family":"Vriesema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"van der Geize","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristof","family":"Cokelaere","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Vercooren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrien","family":"Crul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"R\u00fcdiger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Buchm\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Reijans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Jans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.yexmp.2015.09.004_bb0005","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/S0898-6568(02)00139-0","article-title":"Mechanisms of regulating the Raf kinase family","volume":"15","author":"Chong","year":"2003","journal-title":"Cell. Signal."},{"key":"10.1016\/j.yexmp.2015.09.004_bb0010","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1038\/nature00766","article-title":"Mutations of the BRAF gene in human cancer","volume":"417","author":"Davies","year":"2002","journal-title":"Nature"},{"key":"10.1016\/j.yexmp.2015.09.004_bb0015","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1177\/107327481402100307","article-title":"BRAF mutations: signaling, epidemiology, and clinical experience in multiple malignancies","volume":"21","author":"Hall","year":"2014","journal-title":"Cancer Control"},{"key":"10.1016\/j.yexmp.2015.09.004_bb0020","doi-asserted-by":"crossref","first-page":"3242","DOI":"10.1158\/1078-0432.CCR-12-0052","article-title":"Distinguishing clinicopathologic features of patients with V600E and V600K BRAF-mutant metastatic melanoma","volume":"18","author":"Menzies","year":"2012","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.yexmp.2015.09.004_bb0025","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1016\/j.ejca.2012.12.027","article-title":"Cancer incidence and mortality patterns in Europe: estimates for 40 countries in 2012","volume":"49","author":"Ferlay","year":"2013","journal-title":"Eur. J. Cancer"},{"key":"10.1016\/j.yexmp.2015.09.004_bb0030","doi-asserted-by":"crossref","first-page":"5","DOI":"10.3322\/caac.21254","article-title":"Cancer statistics, 2015","volume":"65","author":"Siegel","year":"2015","journal-title":"CA Cancer J. Clin."},{"key":"10.1016\/j.yexmp.2015.09.004_bb0035","doi-asserted-by":"crossref","first-page":"6199","DOI":"10.1200\/JCO.2009.23.4799","article-title":"Sondak VK: final version of 2009 AJCC melanoma staging and classification","volume":"27","author":"Balch","year":"2009","journal-title":"J. Clin. Oncol."},{"key":"10.1016\/j.yexmp.2015.09.004_bb0040","doi-asserted-by":"crossref","first-page":"157","DOI":"10.2147\/OTT.S39096","article-title":"BRAF-mutant melanoma: treatment approaches, resistance mechanisms, and diagnostic strategies","volume":"8","author":"Spagnolo","year":"2015","journal-title":"Onco. Targets Ther."},{"key":"10.1016\/j.yexmp.2015.09.004_bb0045","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1016\/j.ejca.2012.11.004","article-title":"BRAF inhibitor activity in V600R metastatic melanoma","volume":"49","author":"Klein","year":"2013","journal-title":"Eur. J. Cancer"},{"key":"10.1016\/j.yexmp.2015.09.004_bb0050","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1111\/jcpt.12229","article-title":"Response to MAPK pathway inhibitors in BRAF V600M-mutated metastatic melanoma","volume":"40","author":"Parakh","year":"2015","journal-title":"J. Clin. Pharm. Ther."},{"key":"10.1016\/j.yexmp.2015.09.004_bb0055","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1136\/jclinpath-2012-201345","article-title":"The somatic affairs of BRAF: tailored therapies for advanced malignant melanoma and orphan non-V600E (V600R-M) mutations","volume":"66","author":"Ponti","year":"2013","journal-title":"J. Clin. Pathol."},{"key":"10.1016\/j.yexmp.2015.09.004_bb0060","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.jmoldx.2012.09.001","article-title":"Detection of BRAF p.V600E mutations in melanomas: comparison of four methods argues for sequential use of immunohistochemistry and pyrosequencing","volume":"15","author":"Colomba","year":"2013","journal-title":"J. Mol. Diagn."},{"key":"10.1016\/j.yexmp.2015.09.004_bb0065","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1471-2407-14-13","article-title":"Comparison of high resolution melting analysis, pyrosequencing, next generation sequencing and immunohistochemistry to conventional Sanger sequencing for the detection of p.V600E and non-p.V600E BRAF mutations","volume":"14","author":"Ihle","year":"2014","journal-title":"BMC Cancer"},{"key":"10.1016\/j.yexmp.2015.09.004_bb0070","article-title":"BRAF mutation testing with a rapid and fully integrated molecular diagnostics system","author":"Janku","year":"2015","journal-title":"Clin. Chem."},{"key":"10.1016\/j.yexmp.2015.09.004_bb0075","article-title":"Multicenter evaluation of a novel, automated PCR-based system for rapid detection of BRAF status in formalin-fixed, paraffin embedded tissues","author":"Schiefer","year":"2015","journal-title":"Mod. Pathol."},{"key":"10.1016\/j.yexmp.2015.09.004_bb0080","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0017791","article-title":"Rapid KRAS, EGFR, BRAF and PIK3CA mutation analysis of fine needle aspirates from non-small-cell lung cancer using allele-specific qPCR","volume":"6","author":"van Eijk","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.yexmp.2015.09.004_bb0085","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.5858\/arpa.2011-0505-OA","article-title":"Multisite analytic performance studies of a real-time polymerase chain reaction assay for the detection of BRAF V600E mutations in formalin-fixed, paraffin-embedded tissue specimens of malignant melanoma","volume":"136","author":"Anderson","year":"2012","journal-title":"Arch. Pathol. Lab. Med."},{"key":"10.1016\/j.yexmp.2015.09.004_bb0090","doi-asserted-by":"crossref","first-page":"2507","DOI":"10.1056\/NEJMoa1103782","article-title":"BRIM-3 Study Group: improved survival with vemurafenib in melanoma with BRAF V600E mutation","volume":"364","author":"Chapman","year":"2011","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.yexmp.2015.09.004_bb0095","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S1470-2045(14)70012-9","article-title":"Safety and efficacy of vemurafenib in BRAF(V600E) and BRAF(V600K) mutation-positive melanoma (BRIM-3): extended follow-up of a phase 3, randomised, open-label study","volume":"15","author":"McArthur","year":"2014","journal-title":"Lancet Oncol."},{"key":"10.1016\/j.yexmp.2015.09.004_bb0100","doi-asserted-by":"crossref","first-page":"1893","DOI":"10.1016\/S0140-6736(12)60398-5","article-title":"Dabrafenib in patients with melanoma, untreated brain metastases, and other solid tumours: a phase 1 dose-escalation trial","volume":"379","author":"Falchook","year":"2012","journal-title":"Lancet"},{"key":"10.1016\/j.yexmp.2015.09.004_bb0105","doi-asserted-by":"crossref","first-page":"5518","DOI":"10.1158\/0008-5472.CAN-10-0646","article-title":"RG7204 (PLX4032), a selective BRAFV600E inhibitor, displays potent antitumor activity in preclinical melanoma models","volume":"70","author":"Yang","year":"2010","journal-title":"Cancer Res."},{"key":"10.1016\/j.yexmp.2015.09.004_bb0110","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/1756-8722-5-60","article-title":"Overwhelming response to dabrafenib in a patient with double BRAF mutation (V600E; V600M) metastatic malignant melanoma","volume":"5","author":"Ponti","year":"2012","journal-title":"J. Hematol. Oncol."}],"container-title":["Experimental and Molecular Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014480015001963?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014480015001963?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,8,31]],"date-time":"2019-08-31T00:32:58Z","timestamp":1567211578000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014480015001963"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["S0014480015001963"],"URL":"https:\/\/doi.org\/10.1016\/j.yexmp.2015.09.004","relation":{},"ISSN":["0014-4800"],"issn-type":[{"value":"0014-4800","type":"print"}],"subject":[],"published":{"date-parts":[[2015,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Multi-center evaluation of the novel fully-automated PCR-based Idylla\u2122 BRAF Mutation Test on formalin-fixed paraffin-embedded tissue of malignant melanoma","name":"articletitle","label":"Article Title"},{"value":"Experimental and Molecular Pathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.yexmp.2015.09.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}