{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:39:14Z","timestamp":1791344354700,"version":"4.3.1"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2014,5,1]],"date-time":"2014-05-01T00:00:00Z","timestamp":1398902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2014,5,1]],"date-time":"2014-05-01T00:00:00Z","timestamp":1398902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["General and Comparative Endocrinology"],"published-print":{"date-parts":[[2014,5]]},"DOI":"10.1016\/j.ygcen.2014.02.016","type":"journal-article","created":{"date-parts":[[2014,3,4]],"date-time":"2014-03-04T18:30:33Z","timestamp":1393957833000},"page":"35-43","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":19,"special_numbering":"C","title":["Stallion spermatozoa: Putative target of estrogens; presence of the estrogen receptors ESR1, ESR2 and identification of the estrogen-membrane receptor GPER"],"prefix":"10.1016","volume":"200","author":[{"given":"Brahim","family":"Arkoun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Gautier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Delalande","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Barrier-Battut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Gu\u00e9non","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Goux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Boura\u00efma-Lelong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ygcen.2014.02.016_b0005","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1177\/43.6.7769228","article-title":"Immunohistochemical localization of cytochrome P450 aromatase in equine gonads","volume":"43","author":"Almadhidi","year":"1995","journal-title":"J. Histochem. Cytochem."},{"issue":"3","key":"10.1016\/j.ygcen.2014.02.016_b0010","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.ygcen.2014.02.016_b0015","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1210\/jc.2003-031681","article-title":"Estrogen receptor (ER)alpha and ER beta are both expressed in human ejaculated spermatozoa: evidence of their direct interaction with phosphatidylinositol-3-OH kinase\/Akt pathway","volume":"89","author":"Aquila","year":"2004","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"10.1016\/j.ygcen.2014.02.016_b0020","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1387\/ijdb.072535kb","article-title":"Mobilisation of stored calcium in the neck region of human sperm \u2013 a mechanism for regulation of flagellar activity","volume":"52","author":"Bedu-Addo","year":"2008","journal-title":"Int. J. Dev. Biol."},{"key":"10.1016\/j.ygcen.2014.02.016_b0025","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1038\/aja.2010.78","article-title":"Role and regulation of EGFR in actin remodeling in sperm capacitation and the acrosome reaction","volume":"13","author":"Breitbart","year":"2011","journal-title":"Asian J. Androl."},{"key":"10.1016\/j.ygcen.2014.02.016_b0030","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein\u2013dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"issue":"193","key":"10.1016\/j.ygcen.2014.02.016_b0205","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.ygcen.2013.07.018","article-title":"Nuclear size as estrogen-responsive chromatin quality parameter of mouse spermatozoa","volume":"1","author":"Cacciola","year":"2013","journal-title":"Gen Comp Endocrinol."},{"key":"10.1016\/j.ygcen.2014.02.016_b0035","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1098\/rstb.2009.0235","article-title":"Oestrogens and spermatogenesis","volume":"365","author":"Carreau","year":"2010","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"10.1016\/j.ygcen.2014.02.016_b0040","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.mce.2010.01.035","article-title":"17 Beta-estradiol activates rapid signaling pathways involved in rat pachytene spermatocytes apoptosis through GPR30 and ER alpha","volume":"320","author":"Chimento","year":"2010","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.ygcen.2014.02.016_b0045","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1111\/j.1365-2605.2010.01100.x","article-title":"Gper and ESRs are expressed in rat round spermatids and mediate oestrogen-dependent rapid pathways modulating expression of cyclin B1 and Bax","volume":"34","author":"Chimento","year":"2011","journal-title":"Int. J. Androl."},{"key":"10.1016\/j.ygcen.2014.02.016_b0050","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/1477-7827-8-87","article-title":"Effect of estrogens on boar sperm capacitation in vitro","volume":"8","author":"Ded","year":"2010","journal-title":"Reprod. Biol. Endocrinol."},{"issue":"1","key":"10.1016\/j.ygcen.2014.02.016_b0055","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1161\/01.CIR.0000043805.11780.F5","article-title":"Truncated estrogen receptor alpha 46-kDa isoform in human endothelial cells: relationship to acute activation of nitric oxide synthase","volume":"107","author":"Figtree","year":"2003","journal-title":"Circulation"},{"key":"10.1016\/j.ygcen.2014.02.016_b0060","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1186\/1477-7827-9-134","article-title":"Dose-response effects of estrogenic mycotoxins (zearalenone, alpha- and beta-zearalenol) on motility, hyperactivation and the acrosome reaction of stallion sperm","volume":"9","author":"Filannino","year":"2011","journal-title":"Reprod. Biol. Endocrinol."},{"key":"10.1016\/j.ygcen.2014.02.016_b0065","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1093\/humrep\/dei486","article-title":"Effects of estrogenic xenobiotics on human and mouse spermatozoa","volume":"21","author":"Fraser","year":"2006","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.ygcen.2014.02.016_b0070","doi-asserted-by":"crossref","first-page":"3504","DOI":"10.1210\/en.2012-1200","article-title":"G protein-coupled receptor 30 mediates estrogen-induced proliferation of primordial germ cells via EGFR\/Akt\/\u03b2-catenin signaling pathway","volume":"153","author":"Ge","year":"2012","journal-title":"Endocrinology"},{"key":"10.1016\/j.ygcen.2014.02.016_b0075","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.mce.2011.09.026","article-title":"Estrogen dependent signaling in reproductive tissues \u2013 a role for estrogen receptors and estrogen related receptors","volume":"348","author":"Gibson","year":"2012","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.ygcen.2014.02.016_b0080","doi-asserted-by":"crossref","first-page":"3403","DOI":"10.1002\/jcp.22703","article-title":"Human sperm physiology: estrogen receptor alpha (ER\u03b1) and estrogen receptor beta (ER\u03b2) influence sperm metabolism and may be involved in the pathophysiology of varicocele-associated male infertility","volume":"226","author":"Guido","year":"2011","journal-title":"J. Cell. Physiol."},{"issue":"12","key":"10.1016\/j.ygcen.2014.02.016_b0085","doi-asserted-by":"crossref","first-page":"4489","DOI":"10.1210\/en.2011-1470","article-title":"Minireview: recent advances in extranuclear steroid receptor actions","volume":"152","author":"Hammes","year":"2011","journal-title":"Endocrinology"},{"key":"10.1016\/j.ygcen.2014.02.016_b0090","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1016\/j.domaniend.2005.03.002","article-title":"Expression of aromatase and oestrogen receptors in reproductive tissues of the stallion and a single cryptorchid visualised by means of immunohistochemistry","volume":"29","author":"Hejmej","year":"2005","journal-title":"Domest. Anim. Endocrinol."},{"issue":"2\u20133","key":"10.1016\/j.ygcen.2014.02.016_b0095","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/S0093-691X(03)00237-1","article-title":"Immunocytochemical localization of cytochrome P450 aromatase in the testis of prepubertal, pubertal, and postpubertal horses","volume":"61","author":"Hess","year":"2004","journal-title":"Theriogenology"},{"key":"10.1016\/j.ygcen.2014.02.016_b0100","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0378-4320(99)00050-0","article-title":"Testicular endocrine function, seasonality and semen quality of the stallion","volume":"57","author":"Hoffmann","year":"1999","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.ygcen.2014.02.016_b0105","series-title":"Colloidal Gold: Principles, Methods and Applications","first-page":"75","article-title":"Lowicryls and low temperature embedding for colloidal gold methods","author":"Hobot","year":"1989"},{"key":"10.1016\/j.ygcen.2014.02.016_b0110","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1677\/jme.0.0320279","article-title":"Human immature germ cells and ejaculated spermatozoa contain aromatase and oestrogen receptors","volume":"32","author":"Lambard","year":"2004","journal-title":"J. Mol. Endocrinol."},{"key":"10.1016\/j.ygcen.2014.02.016_b0115","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1006\/gcen.2001.7747","article-title":"Free and conjugated estrogens and androgens in stallion semen","volume":"125","author":"Lemazurier","year":"2002","journal-title":"Gen. Comp. Endocrinol."},{"issue":"Pt 21","key":"10.1016\/j.ygcen.2014.02.016_b0120","doi-asserted-by":"crossref","first-page":"5019","DOI":"10.1113\/jphysiol.2009.177097","article-title":"Membrane oestrogen receptor alpha signalling to cell functions","volume":"587","author":"Levin","year":"2009","journal-title":"J. Physiol."},{"key":"10.1016\/j.ygcen.2014.02.016_b0125","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1095\/biolreprod.108.070250","article-title":"Identification of a membrane estrogen receptor in zebrafish with homology to mammalian GPER and its high expression in early germ cells of the testis","volume":"80","author":"Liu","year":"2009","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.ygcen.2014.02.016_b0130","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1095\/biolreprod.108.074880","article-title":"Hyperactivation of stallion sperm is required for successful in vitro fertilization of equine oocytes","volume":"81","author":"McPartlin","year":"2009","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.ygcen.2014.02.016_b0135","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1016\/j.theriogenology.2010.11.028","article-title":"Xenoestrogenic compounds promote capacitation and an acrosome reaction in porcine sperm","volume":"75","author":"Mohamed","year":"2011","journal-title":"Theriogenology"},{"key":"10.1016\/j.ygcen.2014.02.016_b0140","doi-asserted-by":"crossref","first-page":"3410","DOI":"10.1210\/en.2007-1663","article-title":"Estrogen signaling characteristics of Atlantic croaker G protein-coupled receptor 30 (GPR30) and evidence it is involved in maintenance of oocyte meiotic arrest","volume":"149","author":"Pang","year":"2008","journal-title":"Endocrinology"},{"key":"10.1016\/j.ygcen.2014.02.016_b0145","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/j.theriogenology.2005.12.013","article-title":"Immunolocalization of estrogen and androgen receptors and steroid concentrations in the stallion epididymis","volume":"66","author":"Parlevliet","year":"2006","journal-title":"Theriogenology"},{"key":"10.1016\/j.ygcen.2014.02.016_b0150","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.anireprosci.2011.03.007","article-title":"Immunolocalization of estrogen receptor alpha, estrogen receptor beta and androgen receptor in the pre-, peri- and post-pubertal stallion testis","volume":"125","author":"Pearl","year":"2011","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.ygcen.2014.02.016_b0155","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1095\/biolreprod.110.088765","article-title":"Involvement of epidermal growth factor receptor signaling in estrogen inhibition of oocyte maturation mediated through the G protein-coupled estrogen receptor (Gper) in zebrafish (Danio rerio)","volume":"85","author":"Peyton","year":"2011","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.ygcen.2014.02.016_b0160","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1038\/nrendo.2011.122","article-title":"The G-protein-coupled estrogen receptor GPER in health and disease","volume":"7","author":"Prossnitz","year":"2011","journal-title":"Nat. Rev. Endocrinol."},{"key":"10.1016\/j.ygcen.2014.02.016_b0165","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1139\/o69-124","article-title":"The isolation of estrone sulfate and estradiol-17 beta sulfate from stallion testes","volume":"47","author":"Raeside","year":"1969","journal-title":"Can. J. Biochem."},{"key":"10.1016\/j.ygcen.2014.02.016_b0170","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/1477-7827-5-23","article-title":"Cytochrome P450arom, androgen and estrogen receptors in pig sperm","volume":"5","author":"Rago","year":"2007","journal-title":"Reprod. Biol. Endocrinol."},{"key":"10.1016\/j.ygcen.2014.02.016_b0175","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/1477-7827-4-36","article-title":"Detection of estrogen receptors ER-alpha and ER-beta in human ejaculated immature spermatozoa with excess residual cytoplasm","volume":"4","author":"Rago","year":"2006","journal-title":"Reprod. Biol. Endocrinol."},{"issue":"2","key":"10.1016\/j.ygcen.2014.02.016_b0180","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1095\/biolreprod65.2.462","article-title":"Evaluation of in vitro capacitation of stallion spermatozoa","volume":"65","author":"Rathi","year":"2001","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.ygcen.2014.02.016_b0185","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1530\/REP-11-0326","article-title":"The slower the better: how sperm capacitation and acrosome reaction is modified in the presence of estrogens","volume":"143","author":"Sebkova","year":"2011","journal-title":"Reproduction"},{"key":"10.1016\/j.ygcen.2014.02.016_b0190","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1177\/002215540305100306","article-title":"Immunolocalization of aromatase in stallion Leydig cells and seminiferous tubules","volume":"51","author":"Sipahutar","year":"2003","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.ygcen.2014.02.016_b0195","doi-asserted-by":"crossref","first-page":"3481","DOI":"10.1093\/humrep\/dei267","article-title":"Estrogen receptors alpha and beta (ERalpha and ERbeta) and androgen receptor (AR) in human sperm: localization of ERbeta and AR in mitochondria of the midpiece","volume":"20","author":"Solakidi","year":"2005","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.ygcen.2014.02.016_b0200","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1038\/aja.2010.69","article-title":"Ion channels, phosphorylation and mammalian sperm capacitation","volume":"13","author":"Visconti","year":"2011","journal-title":"Asian J. Androl."}],"container-title":["General and Comparative Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0016648014000598?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0016648014000598?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T18:06:45Z","timestamp":1758046005000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0016648014000598"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5]]},"references-count":41,"alternative-id":["S0016648014000598"],"URL":"https:\/\/doi.org\/10.1016\/j.ygcen.2014.02.016","relation":{},"ISSN":["0016-6480"],"issn-type":[{"value":"0016-6480","type":"print"}],"subject":[],"published":{"date-parts":[[2014,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Stallion spermatozoa: Putative target of estrogens; presence of the estrogen receptors ESR1, ESR2 and identification of the estrogen-membrane receptor GPER","name":"articletitle","label":"Article Title"},{"value":"General and Comparative Endocrinology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ygcen.2014.02.016","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}