{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:20:09Z","timestamp":1787509209288,"version":"build-2736575974"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100017405","name":"Kafrelsheikh University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017405","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["General and Comparative Endocrinology"],"published-print":{"date-parts":[[2016,10]]},"DOI":"10.1016\/j.ygcen.2016.08.016","type":"journal-article","created":{"date-parts":[[2016,9,6]],"date-time":"2016-09-06T10:08:29Z","timestamp":1473156509000},"page":"131-139","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"special_numbering":"C","title":["Is really endogenous ghrelin a hunger signal in chickens? Association of GHSR SNPs with increase appetite, growth traits, expression and serum level of GHRL, and GH"],"prefix":"10.1016","volume":"237","author":[{"given":"Mohammed Abu","family":"El-Magd","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayman A.","family":"Saleh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamer M.","family":"Abdel-Hamid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasha M.","family":"Saleh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed A.","family":"Afifi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ygcen.2016.08.016_b0005","first-page":"147","article-title":"\u201cEl-Salam\u201d a new locally developed strain of chickens","volume":"61","author":"Abd El-Gawad","year":"1983","journal-title":"Agric. Res. Rev."},{"key":"10.1016\/j.ygcen.2016.08.016_b0010","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1007\/s11250-014-0588-3","article-title":"Association of a novel SNP in exon 10 of the IGF2 gene with growth traits in Egyptian water buffalo (Bubalus bubalis)","volume":"46","author":"Abo-Al-Ela","year":"2014","journal-title":"Trop. Anim. Health Prod."},{"key":"10.1016\/j.ygcen.2016.08.016_b0015","doi-asserted-by":"crossref","first-page":"113","DOI":"10.5194\/aab-59-113-2016","article-title":"Effects of peripheral administration of ghrelin antagonist [D-Lys3]-GHRP-6 on growth performance and blood biochemical indices in broiler chickens","volume":"59","author":"Aghdam Shahryar","year":"2016","journal-title":"Arch. Anim. Breed."},{"key":"10.1016\/j.ygcen.2016.08.016_b0020","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1677\/joe.0.1720117","article-title":"Ghrelin: a hypothalamic GH-releasing factor in domestic fowl (Gallus domesticus)","volume":"172","author":"Ahmed","year":"2002","journal-title":"J. Endocrinol."},{"key":"10.1016\/j.ygcen.2016.08.016_b0025","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1093\/bioinformatics\/bth457","article-title":"Haploview: analysis and visualization of LD and haplotype maps","volume":"21","author":"Barrett","year":"2005","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ygcen.2016.08.016_b0030","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1677\/joe.0.1790097","article-title":"Ghrelin-induced GH secretion in domestic fowl in vivo and in vitro","volume":"179","author":"Baudet","year":"2003","journal-title":"J. Endocrinol."},{"key":"10.1016\/j.ygcen.2016.08.016_b0035","doi-asserted-by":"crossref","first-page":"3299","DOI":"10.1210\/jc.2002-021810","article-title":"Polymorphic variants of insulin-like growth factor I (IGF-I) receptor and phosphoinositide 3-kinase genes affect IGF-I plasma levels and human longevity: cues for an evolutionarily conserved mechanism of life span control","volume":"88","author":"Bonafe","year":"2003","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"10.1016\/j.ygcen.2016.08.016_b0040","first-page":"314","article-title":"Construction of a genetic linkage map in man using restriction fragment length polymorphisms","volume":"32","author":"Botstein","year":"1980","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.ygcen.2016.08.016_b0045","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.nlm.2008.12.008","article-title":"Central ghrelin increases anxiety in the Open Field test and impairs retention memory in a passive avoidance task in neonatal chicks","volume":"91","author":"Carvajal","year":"2009","journal-title":"Neurobiol. Learn. Mem."},{"key":"10.1016\/j.ygcen.2016.08.016_b0050","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1080\/10495398.2013.803478","article-title":"Association of single nucleotide polymorphism of GHSR and TGFB2 genes with growth and body composition traits in sire and dam lines of a broiler chicken","volume":"25","author":"Darzi Niarami","year":"2014","journal-title":"Anim. Biotechnol."},{"key":"10.1016\/j.ygcen.2016.08.016_b0055","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.gene.2014.02.059","article-title":"Effects of a novel SNP of IGF2R gene on growth traits and expression rate of IGF2R and IGF2 genes in gluteus medius muscle of Egyptian buffalo","volume":"540","author":"El-Magd","year":"2014","journal-title":"Gene"},{"key":"10.1016\/j.ygcen.2016.08.016_b0060","first-page":"5","article-title":"DNA extraction from fresh or frozen tissues","volume":"49","author":"Fan","year":"2001","journal-title":"Methods Mol. Med."},{"key":"10.1016\/j.ygcen.2016.08.016_b0065","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/s11033-009-9556-9","article-title":"Associations of GHSR gene polymorphisms with chicken growth and carcass traits","volume":"37","author":"Fang","year":"2010","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.ygcen.2016.08.016_b0070","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0016-6480(03)00250-8","article-title":"Distribution and regulation of chicken growth hormone secretagogue receptor isoforms","volume":"134","author":"Geelissen","year":"2003","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.ygcen.2016.08.016_b0075","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.domaniend.2005.06.005","article-title":"Peripheral ghrelin reduces food intake and respiratory quotient in chicken","volume":"30","author":"Geelissen","year":"2006","journal-title":"Domest. Anim. Endocrinol."},{"key":"10.1016\/j.ygcen.2016.08.016_b0080","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1126\/science.273.5277.974","article-title":"A receptor in pituitary and hypothalamus that functions in growth hormone release","volume":"273","author":"Howard","year":"1996","journal-title":"Science"},{"key":"10.1016\/j.ygcen.2016.08.016_b0085","series-title":"Central Functions of the Ghrelin Receptor","author":"Jeanelle","year":"2014"},{"key":"10.1016\/j.ygcen.2016.08.016_b0090","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.neulet.2012.05.036","article-title":"The role of GABAergic system on the inhibitory effect of ghrelin on food intake in neonatal chicks","volume":"520","author":"Jonaidi","year":"2012","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.ygcen.2016.08.016_b0095","doi-asserted-by":"crossref","first-page":"3454","DOI":"10.1210\/en.2002-220255","article-title":"Chicken ghrelin: purification, cDNA cloning, and biological activity","volume":"143","author":"Kaiya","year":"2002","journal-title":"Endocrinology"},{"key":"10.1016\/j.ygcen.2016.08.016_b0100","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.domaniend.2006.03.006","article-title":"Changes in ghrelin levels of plasma and proventriculus and ghrelin mRNA of proventriculus in fasted and refed layer chicks","volume":"32","author":"Kaiya","year":"2007","journal-title":"Domest. Anim. Endocrinol."},{"key":"10.1016\/j.ygcen.2016.08.016_b0105","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.cbpa.2007.12.004","article-title":"Ghrelin: a multifunctional hormone in non-mammalian vertebrates","volume":"149","author":"Kaiya","year":"2008","journal-title":"Comp. Biochem. Physiol. A: Mol. Integr. Physiol."},{"key":"10.1016\/j.ygcen.2016.08.016_b0110","doi-asserted-by":"crossref","first-page":"81","DOI":"10.3389\/fendo.2013.00081","article-title":"Ghrelin receptors in non-mammalian vertebrates","volume":"4","author":"Kaiya","year":"2013","journal-title":"Front. Endocrinol."},{"key":"10.1016\/j.ygcen.2016.08.016_b0115","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.ygcen.2013.04.014","article-title":"Update on ghrelin biology in birds","volume":"190","author":"Kaiya","year":"2013","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.ygcen.2016.08.016_b0120","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.yfrne.2004.03.002","article-title":"Ghrelin \u2013 a hormone with multiple functions","volume":"25","author":"Korbonits","year":"2004","journal-title":"Front. Neuroendocrinol."},{"key":"10.1016\/j.ygcen.2016.08.016_b0125","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1093\/ps\/86.5.835","article-title":"Polymorphism of growth-correlated genes associated with fatness and muscle fiber traits in chickens","volume":"86","author":"Lei","year":"2007","journal-title":"Poult. Sci."},{"key":"10.1016\/j.ygcen.2016.08.016_b0130","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1016\/j.cellsig.2006.11.011","article-title":"The truncated ghrelin receptor polypeptide (GHS-R1b) acts as a dominant-negative mutant of the ghrelin receptor","volume":"19","author":"Leung","year":"2007","journal-title":"Cell. Signal."},{"key":"10.1016\/j.ygcen.2016.08.016_b0135","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1093\/ps\/86.6.1107","article-title":"Effects of chito-oligosaccharide supplementation on performance, nutrient digestibility, and serum composition in broiler chickens","volume":"86","author":"Li","year":"2007","journal-title":"Poult. Sci."},{"key":"10.1016\/j.ygcen.2016.08.016_b0140","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/s11154-011-9169-1","article-title":"The ghrelin\/GOAT\/GHS-R system and energy metabolism","volume":"12","author":"Lim","year":"2011","journal-title":"Rev. Endocr. Metab. Disord."},{"key":"10.1016\/j.ygcen.2016.08.016_b0145","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1111\/j.1474-9726.2011.00740.x","article-title":"Ablation of ghrelin receptor reduces adiposity and improves insulin sensitivity during aging by regulating fat metabolism in white and brown adipose tissues","volume":"10","author":"Lin","year":"2011","journal-title":"Aging Cell"},{"key":"10.1016\/j.ygcen.2016.08.016_b0150","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1124\/jpet.107.123141","article-title":"Four missense mutations in the ghrelin receptor result in distinct pharmacological abnormalities","volume":"322","author":"Liu","year":"2007","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.ygcen.2016.08.016_b0155","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(\u2212Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.ygcen.2016.08.016_b0160","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.livsci.2013.03.020","article-title":"Effect of in ovo ghrelin administration on hatching results and post-hatching performance of broiler chickens","volume":"154","author":"Lotfi","year":"2013","journal-title":"Livestock Sci."},{"key":"10.1016\/j.ygcen.2016.08.016_b0165","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1038\/35051587","article-title":"A role for ghrelin in the central regulation of feeding","volume":"409","author":"Nakazato","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.ygcen.2016.08.016_b0170","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1186\/1297-9686-37-4-339","article-title":"Identification and characterization of single nucleotide polymorphisms in 12 chicken growth-correlated genes by denaturing high performance liquid chromatography","volume":"37","author":"Nie","year":"2005","journal-title":"Genet. Sel. Evol."},{"key":"10.1016\/j.ygcen.2016.08.016_b0175","first-page":"567120","article-title":"Molecular characterization of the ghrelin and ghrelin receptor genes and effects on fat deposition in chicken and duck","volume":"2009","author":"Nie","year":"2009","journal-title":"J. Biomed. Biotechnol."},{"issue":"Suppl. 7","key":"10.1016\/j.ygcen.2016.08.016_b0180","doi-asserted-by":"crossref","first-page":"S55","DOI":"10.1038\/ijo.2008.239","article-title":"Human obesity as a heritable disorder of the central control of energy balance","volume":"32","author":"O\u2019Rahilly","year":"2008","journal-title":"Int. J. Obes."},{"key":"10.1016\/j.ygcen.2016.08.016_b0185","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.regpep.2004.09.003","article-title":"Inhibitory effect of ghrelin on food intake is mediated by the corticotropin-releasing factor system in neonatal chicks","volume":"125","author":"Saito","year":"2005","journal-title":"Regul. Pept."},{"key":"10.1016\/j.ygcen.2016.08.016_b0190","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.ygcen.2004.12.021","article-title":"Different effects of peripheral and central ghrelin on regulation of food intake in the Japanese quail","volume":"141","author":"Shousha","year":"2005","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.ygcen.2016.08.016_b0195","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1172\/JCI0213300","article-title":"Hypothalamic growth hormone secretagogue receptor regulates growth hormone secretion, feeding, and adiposity","volume":"109","author":"Shuto","year":"2002","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.ygcen.2016.08.016_b0200","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.mce.2012.06.012","article-title":"AMPK and the neuroendocrine regulation of appetite and energy expenditure","volume":"366","author":"Stark","year":"2013","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.ygcen.2016.08.016_b0205","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/S0016-6480(03)00247-8","article-title":"Molecular characterization of chicken growth hormone secretagogue receptor gene","volume":"134","author":"Tanaka","year":"2003","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.ygcen.2016.08.016_b0210","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.physbeh.2013.10.034","article-title":"Ghrelin increases food intake, swimming activity and growth in juvenile brown trout (Salmo trutta)","volume":"124","author":"Tinoco","year":"2014","journal-title":"Physiol. Behav."},{"key":"10.1016\/j.ygcen.2016.08.016_b0215","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.regpep.2004.08.007","article-title":"Ghrelin: a gastric peptide that regulates food intake and energy homeostasis","volume":"126","author":"Ueno","year":"2005","journal-title":"Regul. Pept."},{"key":"10.1016\/j.ygcen.2016.08.016_b0220","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/BF03194612","article-title":"Polymorphisms in coding and regulatory regions of the porcine MYF6 and MYOG genes and expression of the MYF6 gene in m. longissimus dorsi versus productive traits in pigs","volume":"47","author":"Wyszynska-Koko","year":"2006","journal-title":"J. Appl. Genet."},{"key":"10.1016\/j.ygcen.2016.08.016_b0225","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.bbr.2011.02.034","article-title":"Genetic selection for body weight in chickens has altered responses of the brain\u2019s AMPK system to food intake regulation effect of ghrelin, but not obestatin","volume":"221","author":"Xu","year":"2011","journal-title":"Behav. Brain Res."},{"key":"10.1016\/j.ygcen.2016.08.016_b0230","series-title":"POPGENE Version 3.1, Microsoft Window-based Freeware for Population Genetic Analysis","author":"Yeh","year":"1999"},{"key":"10.1016\/j.ygcen.2016.08.016_b0235","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.bbr.2015.02.008","article-title":"Fed and fasted chicks from lines divergently selected for low or high body weight have differential hypothalamic appetite-associated factor mRNA expression profiles","volume":"286","author":"Yi","year":"2015","journal-title":"Behav. Brain Res."}],"container-title":["General and Comparative Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0016648016302544?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0016648016302544?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T18:09:56Z","timestamp":1758046196000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0016648016302544"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10]]},"references-count":47,"alternative-id":["S0016648016302544"],"URL":"https:\/\/doi.org\/10.1016\/j.ygcen.2016.08.016","relation":{},"ISSN":["0016-6480"],"issn-type":[{"value":"0016-6480","type":"print"}],"subject":[],"published":{"date-parts":[[2016,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Is really endogenous ghrelin a hunger signal in chickens? Association of GHSR SNPs with increase appetite, growth traits, expression and serum level of GHRL, and GH","name":"articletitle","label":"Article Title"},{"value":"General and Comparative Endocrinology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ygcen.2016.08.016","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}