{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:10:38Z","timestamp":1760166638876,"version":"build-2065373602"},"reference-count":43,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T00:00:00Z","timestamp":1644364800000},"content-version":"vor","delay-in-days":345,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100020250","name":"Centro de Microscopia Eletr\u00f4nica, Universidade Federal do Paran\u00e1","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020250","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["23038.010041\/2013-13"],"award-info":[{"award-number":["23038.010041\/2013-13"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["309897\/2017\u20133","404141\/2016\u20131"],"award-info":[{"award-number":["309897\/2017\u20133","404141\/2016\u20131"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Genomics"],"published-print":{"date-parts":[[2021,3]]},"DOI":"10.1016\/j.ygeno.2021.01.023","type":"journal-article","created":{"date-parts":[[2021,1,30]],"date-time":"2021-01-30T18:24:24Z","timestamp":1612031064000},"page":"805-814","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Zrg1, a cryptococcal protein associated with regulation of growth in nutrient deprivation conditions"],"prefix":"10.1016","volume":"113","author":[{"given":"Camila","family":"Diehl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ane Wichine Acosta","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uriel Perin","family":"Kinskovski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolau","family":"Sbaraini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael de Oliveira","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia Aline Gr\u00f6hs","family":"Ferrareze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra Lehmkuhl","family":"Gerber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Tereza Ribeiro","family":"de Vasconcelos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Livia","family":"Kmetzsch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilene Henning","family":"Vainstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charley Christian","family":"Staats","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ygeno.2021.01.023_bb0005","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1080\/21505594.2016.1257457","article-title":"New facets of antifungal therapy","volume":"8","author":"Chang","year":"2017","journal-title":"Virulence."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0010","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1038\/nrmicro.2015.6","article-title":"Cryptococcus: from environmental saprophyte to global pathogen","volume":"14","author":"May","year":"2016","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0015","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1016\/S1473-3099(17)30243-8","article-title":"Global burden of disease of HIV-associated cryptococcal meningitis: an updated analysis","volume":"17","author":"Rajasingham","year":"2017","journal-title":"Lancet Infect. Dis."},{"year":"2009","series-title":"Chapter 5 Virulence in Cryptococcus Species","author":"Ma","key":"10.1016\/j.ygeno.2021.01.023_bb0020"},{"key":"10.1016\/j.ygeno.2021.01.023_bb0025","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00946-17","article-title":"Conservation of intracellular pathogenic strategy among distantly related cryptococcal species","volume":"86","author":"Freij","year":"2018","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0030","article-title":"The disease ecology, epidemiology, clinical manifestations, and management of emerging cryptococcus gattii complex infections","author":"Diaz","year":"2019","journal-title":"Wilderness Environ. Med."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0035","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.mib.2016.05.013","article-title":"The roles of zinc and copper sensing in fungal pathogenesis","volume":"32","author":"Ballou","year":"2016","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0040","doi-asserted-by":"crossref","DOI":"10.1093\/femsre\/fux050","article-title":"Metals in fungal virulence","volume":"42","author":"Gerwien","year":"2018","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0045","series-title":"Adv. Microb. Physiol","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/bs.ampbs.2017.01.006","article-title":"Nutritional Immunity and Fungal Pathogenesis: The Struggle for Micronutrients at the Host\u2013Pathogen Interface","author":"Malavia","year":"2017"},{"key":"10.1016\/j.ygeno.2021.01.023_bb0050","doi-asserted-by":"crossref","first-page":"491","DOI":"10.2217\/fmb-2016-0160","article-title":"Alterations of zinc homeostasis in response to Cryptococcus neoformans in a murine macrophage cell line","volume":"12","author":"Dos Santos","year":"2017","journal-title":"Future Microbiol."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0055","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1016\/j.immuni.2013.09.006","article-title":"Granulocyte macrophage-colony stimulating factor induced Zn sequestration enhances macrophage superoxide and limits intracellular pathogen survival","volume":"39","author":"SubramanianVignesh","year":"2013","journal-title":"Immunity."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0060","doi-asserted-by":"crossref","DOI":"10.1039\/C4MT00331D","article-title":"An evolutionary perspective on zinc uptake by human fungal pathogens","author":"Wilson","year":"2015","journal-title":"Metallomics"},{"key":"10.1016\/j.ygeno.2021.01.023_bb0065","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0043773","article-title":"Zap1 Regulates Zinc Homeostasis and Modulates Virulence in Cryptococcus gattii","volume":"7","author":"de Schneider","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.ygeno.2021.01.023_bb0070","doi-asserted-by":"crossref","first-page":"10104","DOI":"10.1038\/srep10104","article-title":"Effects of zinc transporters on Cryptococcus gattii virulence","volume":"5","author":"Schneider","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0075","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2017.01626","article-title":"Modulation of Zinc Homeostasis in Acanthamoeba castellanii as a Possible Antifungal Strategy against Cryptococcus gattii","volume":"8","author":"Ribeiro","year":"2017","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0080","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.R900014200","article-title":"Homeostatic and adaptive responses to zinc deficiency in saccharomyces cerevisiae","author":"Eide","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0085","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2013.00065","article-title":"Fungal zinc metabolism and its connections to virulence","volume":"3","author":"Staats","year":"2013","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0090","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2017.00535","article-title":"The Anti-helminthic Compound Mebendazole Has Multiple Antifungal Effects against Cryptococcus neoformans","volume":"8","author":"Joffe","year":"2017","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0095","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.fgb.2007.11.001","article-title":"DelsGate, a robust and rapid gene deletion construction method","volume":"45","author":"Garc\u00eda-Pedrajas","year":"2008","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0100","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative CT method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."},{"year":"2010","series-title":"FastQC, Babraham Bioinforma","author":"Andrews","key":"10.1016\/j.ygeno.2021.01.023_bb0105"},{"key":"10.1016\/j.ygeno.2021.01.023_bb0110","doi-asserted-by":"crossref","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: Ultrafast universal RNA-seq aligner","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ygeno.2021.01.023_bb0115","article-title":"Application of an optimized annotation pipeline to the Cryptococcus deuterogattii genome reveals dynamic primary metabolic gene clusters and genomic impact of RNAi loss","author":"Ferrareze","year":"2020","journal-title":"BioRxiv"},{"key":"10.1016\/j.ygeno.2021.01.023_bb0120","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0125","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gky1085","article-title":"EggNOG 5.0: A hierarchical, functionally and phylogenetically annotated orthology resource based on 5090 organisms and 2502 viruses","author":"Huerta-Cepas","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0130","doi-asserted-by":"crossref","DOI":"10.1093\/molbev\/msx148","article-title":"Fast genome-wide functional annotation through orthology assignment by eggNOG-mapper","author":"Huerta-Cepas","year":"2017","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0135","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.1093\/bioinformatics\/btl140","article-title":"Improved scoring of functional groups from gene expression data by decorrelating GO graph structure","volume":"22","author":"Alexa","year":"2006","journal-title":"Bioinformatics."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0140","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.2217\/fmb-2016-0090","article-title":"The putative autophagy regulator Atg7 affects the physiology and pathogenic mechanisms of Cryptococcus neoformans","volume":"11","author":"Oliveira","year":"2016","journal-title":"Future Microbiol."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0145","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.mimet.2015.10.004","article-title":"Tenebrio molitor (Coleoptera: Tenebrionidae) as an alternative host to study fungal infections","volume":"118","author":"de Souza","year":"2015","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.ygeno.2021.01.023_bb0150","doi-asserted-by":"crossref","first-page":"39","DOI":"10.3390\/jof4010039","article-title":"FungiDB: an integrated bioinformatic resource for fungi and oomycetes","volume":"4","author":"Basenko","year":"2018","journal-title":"J. Fungi."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0155","doi-asserted-by":"crossref","first-page":"W459","DOI":"10.1093\/nar\/gky320","article-title":"BUSCA: an integrative web server to predict subcellular localization of proteins","volume":"46","author":"Savojardo","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0160","doi-asserted-by":"crossref","first-page":"31582","DOI":"10.1074\/jbc.M506736200","article-title":"Autophagy is required for maintenance of amino acid levels and protein synthesis under nitrogen starvation","volume":"280","author":"Onodera","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0165","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2019.02690","article-title":"Conserved autophagy pathway contributes to stress tolerance and virulence and differentially controls Autophagic flux upon nutrient starvation in Cryptococcus neoformans","volume":"10","author":"Zhao","year":"2019","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0175","doi-asserted-by":"crossref","first-page":"49","DOI":"10.3390\/microorganisms5030049","article-title":"Transcriptional analysis allows genome reannotation and reveals that Cryptococcus gattii VGII undergoes nutrient restriction during infection","volume":"5","author":"Ferrareze","year":"2017","journal-title":"Microorganisms."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0180","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.idc.2015.10.004","article-title":"The global burden of fungal diseases","volume":"30","author":"Vallabhaneni","year":"2016","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0185","doi-asserted-by":"crossref","first-page":"e367","DOI":"10.1016\/S1473-3099(17)30306-7","article-title":"Fungal infections 4 neglected endemic mycoses","volume":"17","author":"Falci","year":"2017","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0190","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.abb.2016.02.031","article-title":"Zinc sensing and regulation in yeast model systems","volume":"611","author":"Wilson","year":"2016","journal-title":"Arch. Biochem. Biophys."},{"year":"2004","series-title":"Review Development by Self-Digestion: Molecular Mechanisms and Biological Functions of Autophagy for the degradation of short-lived proteins, autophagy is the primary intracellular catabolic mechanism for de-grading and recycling long-lived proteins and o","author":"Levine","key":"10.1016\/j.ygeno.2021.01.023_bb0195"},{"key":"10.1016\/j.ygeno.2021.01.023_bb0200","doi-asserted-by":"crossref","first-page":"8520","DOI":"10.1074\/jbc.M116.762948","article-title":"Zinc starvation induces autophagy in yeast","volume":"292","author":"Kawamata","year":"2017","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0205","doi-asserted-by":"crossref","DOI":"10.1080\/15548627.2015.1099796","article-title":"A large-scale analysis of autophagy-related gene expression identifies new regulators of autophagy","author":"Bernard","year":"2015","journal-title":"Autophagy"},{"key":"10.1016\/j.ygeno.2021.01.023_bb0210","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1003715","article-title":"Global analysis of fission yeast mating genes reveals new autophagy factors","volume":"9","author":"Sun","year":"2013","journal-title":"PLoS Genet."},{"key":"10.1016\/j.ygeno.2021.01.023_bb0215","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00069-18","article-title":"ATG genes influence the virulence of Cryptococcus neoformans through contributions beyond core autophagy functions","author":"Ding","year":"2018","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ygeno.2021.01.023_bib216","doi-asserted-by":"crossref","first-page":"103438","DOI":"10.1016\/j.fgb.2020.103438","article-title":"Participation of Zip3, a ZIP domain-containing protein, in stress response and virulence in Cryptococcus gattii","volume":"144","author":"Garcia","year":"2020","journal-title":"Fungal Genetics and Biology"}],"container-title":["Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0888754321000495?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0888754321000495?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:29:53Z","timestamp":1760164193000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0888754321000495"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3]]},"references-count":43,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,3]]}},"alternative-id":["S0888754321000495"],"URL":"https:\/\/doi.org\/10.1016\/j.ygeno.2021.01.023","relation":{},"ISSN":["0888-7543"],"issn-type":[{"type":"print","value":"0888-7543"}],"subject":[],"published":{"date-parts":[[2021,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Zrg1, a cryptococcal protein associated with regulation of growth in nutrient deprivation conditions","name":"articletitle","label":"Article Title"},{"value":"Genomics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ygeno.2021.01.023","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Inc.","name":"copyright","label":"Copyright"}]}}