{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:06:57Z","timestamp":1791216417418,"version":"4.1.0"},"reference-count":61,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand (NRCT), Thailand","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Metabolic Engineering"],"published-print":{"date-parts":[[2015,7]]},"DOI":"10.1016\/j.ymben.2015.04.004","type":"journal-article","created":{"date-parts":[[2015,4,17]],"date-time":"2015-04-17T19:33:10Z","timestamp":1429299190000},"page":"16-26","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":82,"special_numbering":"C","title":["Efficient reduction of the formation of by-products and improvement of production yield of 2,3-butanediol by a combined deletion of alcohol dehydrogenase, acetate kinase-phosphotransacetylase, and lactate dehydrogenase genes in metabolically engineered Klebsiella oxytoca in mineral salts medium"],"prefix":"10.1016","volume":"30","author":[{"given":"Kaemwich","family":"Jantama","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pattharasedthi","family":"Polyiam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panwana","family":"Khunnonkwao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sitha","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maytawadee","family":"Sangproo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirin","family":"Khor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirima Suvarnakuta","family":"Jantama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunthorn","family":"Kanchanatawee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymben.2015.04.004_bib1","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1099\/00221287-147-6-1483","article-title":"Pyruvate oxidase contributes to the aerobic growth efficiency of Escherichia coli","volume":"147","author":"Abdel-Hamid","year":"2001","journal-title":"Microbiology"},{"key":"10.1016\/j.ymben.2015.04.004_bib2","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1093\/clinchem\/9.4.391","article-title":"Serum lactic dehydrogenase activity: an analytical assessment of current assays","volume":"12","author":"Amador","year":"1963","journal-title":"Clin. Chem."},{"issue":"1","key":"10.1016\/j.ymben.2015.04.004_bib3","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1128\/JB.185.1.204-209.2003","article-title":"Requirement of ArcA for redox regulation in Escherichia coli under microaerobic but not anaerobic or aerobic conditions","volume":"185","author":"Alexeeva","year":"2003","journal-title":"J. Bacteriol."},{"issue":"4","key":"10.1016\/j.ymben.2015.04.004_bib4","doi-asserted-by":"crossref","first-page":"e1003427","DOI":"10.1371\/journal.pgen.1003427","article-title":"Evolution after introduction of a novel metabolic pathway consistently leads to restoration of wild-type physiology","volume":"9","author":"Carroll","year":"2013","journal-title":"PloS Genet."},{"key":"10.1016\/j.ymben.2015.04.004_bib5","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/j.biotechadv.2009.05.002","article-title":"Biotechnological production of 2,3-butanediol-current state and prospects","volume":"27","author":"Celinska","year":"2009","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.ymben.2015.04.004_bib6","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1007\/s00449-012-0691-7","article-title":"Fermentation and evaluation of Klebsiella pneumonia and K. oxytoca on the production of 2,3-butanediol","volume":"35","author":"Cho","year":"2012","journal-title":"Bioprocess. Biosyst. Eng."},{"key":"10.1016\/j.ymben.2015.04.004_bib7","doi-asserted-by":"crossref","first-page":"e1000652","DOI":"10.1371\/journal.pgen.1000652","article-title":"Fast growth increases the selective advantage of a mutation arising recurrently during evolution under metal limitation","volume":"5","author":"Chou","year":"2009","journal-title":"PLoS Genet."},{"key":"10.1016\/j.ymben.2015.04.004_bib8","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1073\/pnas.0334340100","article-title":"Parallel changes in gene expression after 20,000 generations of evolution in Escherichia coli","volume":"100","author":"Cooper","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"11","key":"10.1016\/j.ymben.2015.04.004_bib9","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1007\/s10295-007-0244-2","article-title":"Minimizing acetate formation in E. coli fermentations","volume":"34","author":"De Mey","year":"2007","journal-title":"J. Ind. Microbiol. Biotechnol."},{"issue":"24","key":"10.1016\/j.ymben.2015.04.004_bib10","doi-asserted-by":"crossref","first-page":"8322","DOI":"10.1128\/JB.187.24.8322-8331.2005","article-title":"The maltodextrin system of Escherichia coli: metabolism and transport","volume":"187","author":"Dippel","year":"2005","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.ymben.2015.04.004_bib11","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.tibtech.2006.09.001","article-title":"Overcoming acetate in Escherichia coli recombinant protein fermentations","volume":"24","author":"Eiteman","year":"2006","journal-title":"Trends Biotechnol."},{"issue":"46","key":"10.1016\/j.ymben.2015.04.004_bib12","doi-asserted-by":"crossref","first-page":"35876","DOI":"10.1074\/jbc.M003035200","article-title":"Characterization of a (2R,3R)-2,3-butanediol dehydrogenase as the Saccharomyces cerevisiae YAL060W gene product","volume":"275","author":"Gonzalez","year":"2000","journal-title":"Biol. Chem."},{"key":"10.1016\/j.ymben.2015.04.004_bib13","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1186\/1754-6834-7-44","article-title":"Effect of the inactivation of lactate dehydrogenase, ethanol dehydrogenase, and phosphotransacetylase on 2,3-butanediol production in Klebsiella pneumoniae strain","volume":"7","author":"Guo","year":"2014","journal-title":"Biotechnol. Biofuels"},{"issue":"15","key":"10.1016\/j.ymben.2015.04.004_bib14","doi-asserted-by":"crossref","first-page":"14780","DOI":"10.1074\/jbc.M414642200","article-title":"Activation of the LicT transcriptional antiterminator involves a domain swing\/lock mechanism provoking massive structural changes","volume":"280","author":"Graille","year":"2005","journal-title":"Biol. Chem."},{"key":"10.1016\/j.ymben.2015.04.004_bib15","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.nbt.2012.09.004","article-title":"Production of 2,3-butanediol by a low-acid producing Klebsiella oxytoca NBRF4","volume":"30","author":"Han","year":"2013","journal-title":"N. Biotechnol."},{"issue":"2","key":"10.1016\/j.ymben.2015.04.004_bib16","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1046\/j.1365-2958.1998.00696.x","article-title":"Novel keto acid formate-lyase and propionate kinase enzymes are components of an anaerobic pathway in Escherichia coli that degrades L threonine to propionate","volume":"27","author":"Hesslinger","year":"1998","journal-title":"Mol. Microbiol."},{"issue":"5","key":"10.1016\/j.ymben.2015.04.004_bib17","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1093\/oxfordjournals.jbchem.a133597","article-title":"Regulation of Escherichia coli phosphoenolpyruvate carboxylase by multiple effectors in vivo. II. Kinetic studies with a reaction system containing physiological concentrations of ligands","volume":"90","author":"Izui","year":"1981","journal-title":"J. Biochem."},{"key":"10.1016\/j.ymben.2015.04.004_bib18","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1002\/bit.22005","article-title":"Eliminating side products and increasing succinate yields in engineered strains of Escherichia coli C","volume":"101","author":"Jantama","year":"2008","journal-title":"Biotechnol. Bioeng."},{"issue":"12","key":"10.1016\/j.ymben.2015.04.004_bib19","doi-asserted-by":"crossref","first-page":"4659","DOI":"10.1073\/pnas.0804943106","article-title":"The O2 sensitivity of the transcription factor FNR is controlled by Ser24 modulating the kinetics of [4Fe\u20134S] to [2Fe\u20132S] conversion","volume":"106","author":"Jervis","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ymben.2015.04.004_bib20","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1007\/s00253-009-2222-2","article-title":"Engineering Klebsiella oxytoca for efficient 2,3-butanediol production through insertional inactivation of acetaldehyde dehydrogenase gene","volume":"85","author":"Ji","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2015.04.004_bib21","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1128\/JB.123.3.1124-1130.1975","article-title":"Physiological and biochemical role of the butanediol pathway in Aerobacter (Enterobacter) aerogenes","volume":"123","author":"Johansen","year":"1975","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.ymben.2015.04.004_bib22","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1007\/s00253-012-3883-9","article-title":"Deletion of lactate dehydrogenase in Enterobacter aerogenes to enhance 2,3-butanediol production","volume":"95","author":"Jung","year":"2012","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2015.04.004_bib23","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.biortech.2013.04.003","article-title":"Engineered Enterobacter aerogenes for efficient utilization of sugarcane molasses in 2,3-butanediol production","volume":"139","author":"Jung","year":"2013","journal-title":"Bioresour. Technol."},{"issue":"1","key":"10.1016\/j.ymben.2015.04.004_bib24","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/S0006-291X(02)00639-3","article-title":"Extensive overproduction of the AdhE protein by rng mutations depends on mutations in the cra gene or in the Cra-box of the adhE promoter","volume":"295","author":"Kaga","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.ymben.2015.04.004_bib25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bej.2005.05.010","article-title":"Effect of ldhA gene deletion on the metabolism of Escherichia coli based on gene expression, enzyme activities, intracellular metabolite concentrations, and metabolic flux distribution","volume":"26","author":"Kabir","year":"2005","journal-title":"Biochem. Eng. J."},{"issue":"9","key":"10.1016\/j.ymben.2015.04.004_bib26","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.4014\/jmb.1201.01044","article-title":"Enhanced 2,3-butanediol production in recombinant Klebsiella pneumonia via overexpression of synthesis-related genes","volume":"22","author":"Kin","year":"2012","journal-title":"Microbiol. Biotechnol."},{"issue":"2","key":"10.1016\/j.ymben.2015.04.004_bib27","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.jbiosc.2013.02.021","article-title":"Metabolic engineering of a novel Klebsiella oxytoca strain for enhanced 2,3-butanediol production","volume":"116","author":"Kim","year":"2013","journal-title":"J. Biosci. Bioeng."},{"key":"10.1016\/j.ymben.2015.04.004_bib28","doi-asserted-by":"crossref","first-page":"5467","DOI":"10.1128\/AEM.00355-11","article-title":"2,3-Butanediol production by acetogenic bacteria, an alternative route to chemical synthesis, using industrial waste gas","volume":"77","author":"Kopke","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ymben.2015.04.004_bib29","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1128\/AEM.72.3.1939-1948.2006","article-title":"Genome-based metabolic engineering of Mannheimia succiniciproducens for succinic acid production","volume":"98","author":"Lee","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"issue":"5","key":"10.1016\/j.ymben.2015.04.004_bib30","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1002\/bit.20381","article-title":"Effect of oxygen on the Escherichia coli ArcA and FNR regulation systems and metabolic responses","volume":"89","author":"Levanon","year":"2005","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.ymben.2015.04.004_bib31","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.ymben.2014.11.010","article-title":"Metabolic engineering of Enterobacter cloacae for high-yield production of enantiopure (2R,3R)-2,3-butanediol from lignocellulose-derived sugars","volume":"28","author":"Li","year":"2015","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2015.04.004_bib32","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/s00253-008-1732-7","article-title":"Enhanced 2,3-butanediol production by Klebsiella pneumoniae SDM","volume":"282","author":"Ma","year":"2009","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2015.04.004_bib33","first-page":"269","article-title":"Microbial production of 2,3-butanediol","volume":"Vol. 6","author":"Maddox","year":"1996"},{"key":"10.1016\/j.ymben.2015.04.004_bib34","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1007\/s10529-006-9252-y","article-title":"Low salt medium for lactate and ethanol production by recombinant Escherichia coli B","volume":"29","author":"Martinez","year":"2007","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.ymben.2015.04.004_bib35","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.jbiotec.2013.06.023","article-title":"Catabolite regulation analysis of Escherichia coli for acetate overflow mechanism and co-consumption of multiple sugars based on systems biology approach using computer simulation","volume":"168","author":"Matsuoka","year":"2013","journal-title":"J. Biotechnol."},{"issue":"5","key":"10.1016\/j.ymben.2015.04.004_bib36","doi-asserted-by":"crossref","first-page":"1808","DOI":"10.1128\/AEM.62.5.1808-1810.1996","article-title":"Enhanced production of succinic acid by overexpression of phosphoenolpyruvate carboxylase in Escherichia coli","volume":"62","author":"Millard","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ymben.2015.04.004_bib37","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.ymben.2014.07.011","article-title":"Establishment of a novel gene expression method, BICES (biomass-inducible chromosome-based expression system), and its application to the production of 2,3-butanediol and acetoin","volume":"25","author":"Nakashima","year":"2014","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2015.04.004_bib38","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1007\/s12010-010-9098-6","article-title":"An effective and simplified fed-batch strategy for improved 2,3-butanediol production by Klebsiella oxytoca","volume":"163","author":"Nie","year":"2011","journal-title":"Appl. Biochem. Biotechnol."},{"issue":"1","key":"10.1016\/j.ymben.2015.04.004_bib39","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1006\/abbi.1996.9748","article-title":"l-malate dehydrogenase from Pseudomonas stutzeri: purification and characterization","volume":"337","author":"Nikolaos","year":"1997","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.ymben.2015.04.004_bib40","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.ymben.2014.01.001","article-title":"Combinatorial optimization of cyanobacterial 2,3-butanediol production","volume":"22","author":"Oliver","year":"2014","journal-title":"Metab. Eng."},{"issue":"22","key":"10.1016\/j.ymben.2015.04.004_bib41","doi-asserted-by":"crossref","first-page":"6652","DOI":"10.1128\/jb.177.22.6652-6656.1995","article-title":"Regulation of malate dehydrogenase (mdh) gene expression in Escherichia coli in response to oxygen, carbon, and heme availability","volume":"177","author":"Park","year":"1995","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.ymben.2015.04.004_bib42","first-page":"12","article-title":"Genome-scale reconstruction and in silico analysis of Klebsiella oxytoca for 2,3-butanediol production","volume":"23","author":"Park","year":"2013","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.ymben.2015.04.004_bib43","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1007\/s10295-013-1298-y","article-title":"In silico aided metabolic engineering of Klebsiella oxytoca and fermentation optimization for enhanced 2,3-butanediol production","volume":"40","author":"Park","year":"2013","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2015.04.004_bib44","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1007\/s00253-009-2004-x","article-title":"High production of 2,3-butanediol from glycerol by Klebsiella pneumoniae G31","volume":"84","author":"Petrov","year":"2009","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"2","key":"10.1016\/j.ymben.2015.04.004_bib45","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S1369-5274(02)00296-5","article-title":"Regulation of gene expression in the PTS in Escherichia coli: the role and interactions of Mlc","volume":"5","author":"Plumbridge","year":"2002","journal-title":"Curr. Opin. Microbiol."},{"issue":"2","key":"10.1016\/j.ymben.2015.04.004_bib46","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1128\/AEM.02544-12","article-title":"Molecular control of sucrose utilization in Escherichia coli W, an efficient sucrose-utilizing strain","volume":"79","author":"Sabri","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"issue":"1","key":"10.1016\/j.ymben.2015.04.004_bib47","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1006\/abbi.1998.0664","article-title":"Aldehyde dehydrogenase (CoA-acetylating) and the mechanism of ethanol formation in the mitochondriate protest Giardia lamblia","volume":"354","author":"Sanchez","year":"1998","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.ymben.2015.04.004_bib48","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.biortech.2012.05.114","article-title":"Metabolic engineering of Klebsiella oxytoca M5a1 to produce optically pure d-lactate in mineral salts medium","volume":"119","author":"Sangproo","year":"2012","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.ymben.2015.04.004_bib49","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.ymben.2013.09.008","article-title":"Synthesis of 2,3-butanediol by Synechocystis sp. PCC6803 via heterologous expression of a catabolic pathway from lactic acid- and enterobacteria","volume":"20","author":"Savakis","year":"2013","journal-title":"Metab. Eng."},{"issue":"2","key":"10.1016\/j.ymben.2015.04.004_bib50","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S1369-5274(99)80034-4","article-title":"Carbon catabolite repression in bacteria","volume":"2","author":"Stulke","year":"1999","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.ymben.2015.04.004_bib51","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1128\/JB.163.3.1074-1079.1985","article-title":"Isolation and expression of the Escherichia coli gene encoding malate dehydrogenase","volume":"163","author":"Sutherland","year":"1985","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.ymben.2015.04.004_bib52","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/JB.140.1.1-13.1979","article-title":"Escherichia coli mutants impaired in maltodextrin transport","volume":"140","author":"Wandersman","year":"1979","journal-title":"J. Bacteriol."},{"issue":"7","key":"10.1016\/j.ymben.2015.04.004_bib53","doi-asserted-by":"crossref","first-page":"e40442","DOI":"10.1371\/journal.pone.0040442","article-title":"Efficient 2,3-butanediol production from cassava powder by a crop-biomass-utilizer, Enterobacter cloacae subsp. dissolvens SDM","volume":"7","author":"Wang","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.ymben.2015.04.004_bib54","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.1002\/bit.24427","article-title":"Metabolic engineering of thermophilic Bacillus licheniformis for chiral pure d-2,3-butanediol production","volume":"9","author":"Wang","year":"2012","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.ymben.2015.04.004_bib55","doi-asserted-by":"crossref","first-page":"1366","DOI":"10.1021\/bp050100e","article-title":"Development of industrial medium required elimination of the 2,3-butanediol fermentation pathway to maintain ethanol yield in an ethanologenic strain of Klebsiella oxytoca","volume":"21","author":"Wood","year":"2005","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.ymben.2015.04.004_bib56","doi-asserted-by":"crossref","first-page":"9107","DOI":"10.1073\/pnas.0602917103","article-title":"Tests of parallel molecular evolution in a long-term experiment with Escherichia coli","volume":"103","author":"Woods","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ymben.2015.04.004_bib57","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.ymben.2014.02.004","article-title":"Systematic metabolic engineering of Escherichia coli for high-yield production of fuel bio-chemical 2,3-butanediol","volume":"23","author":"Xu","year":"2014","journal-title":"Metab. Eng."},{"issue":"2","key":"10.1016\/j.ymben.2015.04.004_bib58","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/(SICI)1097-0290(20000720)69:2<150::AID-BIT4>3.0.CO;2-N","article-title":"Effect of variation of Klebsiella pneumonia acetolactate synthase expressionon metabolic flux redistribution in Escherichia coli","volume":"69","author":"Yang","year":"2000","journal-title":"Biotechnol. Bioeng."},{"issue":"2","key":"10.1016\/j.ymben.2015.04.004_bib59","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.ymben.2008.11.001","article-title":"Introduction of an NADH regeneration system into Klebsiella oxytoca leads to an enhanced oxidative and reductive metabolism of glycerol","volume":"11","author":"Zhang","year":"2009","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2015.04.004_bib60","doi-asserted-by":"crossref","first-page":"1961","DOI":"10.1016\/j.biortech.2009.10.052","article-title":"Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30","volume":"10","author":"Zhang","year":"2010","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.ymben.2015.04.004_bib61","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.1007\/s00253-010-2678-0","article-title":"Expression of 1,3-propanediol oxidoreductase and its isoenzyme in Klebsiella pneumoniae for bioconversion of glycerol into 1,3-propanediol","volume":"87","author":"Zhuge","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."}],"container-title":["Metabolic Engineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096717615000488?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096717615000488?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,8,31]],"date-time":"2020-08-31T21:29:57Z","timestamp":1598909397000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1096717615000488"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7]]},"references-count":61,"alternative-id":["S1096717615000488"],"URL":"https:\/\/doi.org\/10.1016\/j.ymben.2015.04.004","relation":{},"ISSN":["1096-7176"],"issn-type":[{"value":"1096-7176","type":"print"}],"subject":[],"published":{"date-parts":[[2015,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Efficient reduction of the formation of by-products and improvement of production yield of 2,3-butanediol by a combined deletion of alcohol dehydrogenase, acetate kinase-phosphotransacetylase, and lactate dehydrogenase genes in metabolically engineered Klebsiella oxytoca in mineral salts medium","name":"articletitle","label":"Article Title"},{"value":"Metabolic Engineering","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymben.2015.04.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}