{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T13:43:26Z","timestamp":1789134206082,"version":"build-2803163510"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003050","name":"Ministry of Economy, Trade and Industry","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Metabolic Engineering"],"published-print":{"date-parts":[[2020,5]]},"DOI":"10.1016\/j.ymben.2020.01.004","type":"journal-article","created":{"date-parts":[[2020,1,8]],"date-time":"2020-01-08T16:46:04Z","timestamp":1578501964000},"page":"24-35","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":46,"special_numbering":"C","title":["Isobutanol production in Corynebacterium glutamicum: Suppressed succinate by-production by pckA inactivation and enhanced productivity via the Entner\u2013Doudoroff pathway"],"prefix":"10.1016","volume":"59","author":[{"given":"Satoshi","family":"Hasegawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Jojima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masako","family":"Suda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayuki","family":"Inui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymben.2020.01.004_bib1","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1038\/nature06450","article-title":"Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels","volume":"451","author":"Atsumi","year":"2008","journal-title":"Nature"},{"key":"10.1016\/j.ymben.2020.01.004_bib2","doi-asserted-by":"crossref","first-page":"6306","DOI":"10.1128\/AEM.01160-09","article-title":"Acetolactate synthase from Bacillus subtilis serves as a 2-ketoisovalerate decarboxylase for isobutanol biosynthesis in Escherichia coli","volume":"75","author":"Atsumi","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ymben.2020.01.004_bib3","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s00253-009-2085-6","article-title":"Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase\/alcohol dehydrogenase genes","volume":"85","author":"Atsumi","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2020.01.004_bib4","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.ymben.2011.02.004","article-title":"Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli","volume":"13","author":"Bastian","year":"2011","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2020.01.004_bib5","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.jbiotec.2013.11.025","article-title":"The bacterial Entner-Doudoroff pathway does not replace glycolysis in Saccharomyces cerevisiae due to the lack of activity of iron-sulfur cluster enzyme 6-phosphogluconate dehydratase","volume":"171","author":"Benisch","year":"2014","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.ymben.2020.01.004_bib6","doi-asserted-by":"crossref","first-page":"3300","DOI":"10.1128\/AEM.02972-10","article-title":"Corynebacterium glutamicum tailored for efficient isobutanol production","volume":"77","author":"Blombach","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ymben.2020.01.004_bib7","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0168-1656(03)00144-5","article-title":"The respiratory chain of Corynebacterium glutamicum","volume":"104","author":"Bott","year":"2003","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.ymben.2020.01.004_bib8","doi-asserted-by":"crossref","DOI":"10.1093\/femsle\/fnw020","article-title":"Frontiers in microbial 1-butanol and isobutanol production","volume":"363","author":"Chen","year":"2016","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.ymben.2020.01.004_bib9","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01524-14","article-title":"Metabolic engineering of microorganisms for the production of higher alcohols","volume":"5","author":"Choi","year":"2014","journal-title":"mBio"},{"key":"10.1016\/j.ymben.2020.01.004_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.1992.tb05822.x","article-title":"The Entner-Doudoroff pathway: history, physiology and molecular biology","volume":"9","author":"Conway","year":"1992","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.ymben.2020.01.004_bib11","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1073\/pnas.80.1.21","article-title":"The tac promoter: a functional hybrid derived from the trp and lac promoters","volume":"80","author":"de Boer","year":"1983","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"key":"10.1016\/j.ymben.2020.01.004_bib12","first-page":"367","article-title":"Biochemical and molecular characterization of alpha-ketoisovalerate decarboxylase, an enzyme involved in the formation of aldehydes from amino acids by Lactococcus lactis","volume":"238","author":"de la Plaza","year":"2004","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.ymben.2020.01.004_bib13","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1046\/j.1432-1327.1998.2540096.x","article-title":"Carbon-flux distribution in the central metabolic pathways of Corynebacterium glutamicum during growth on fructose","volume":"254","author":"Dominguez","year":"1998","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.ymben.2020.01.004_bib14","doi-asserted-by":"crossref","first-page":"6076","DOI":"10.1128\/jb.174.19.6076-6086.1992","article-title":"Identification, sequence analysis, and expression of a Corynebacterium glutamicum gene cluster encoding the three glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase, and triosephosphate isomerase","volume":"174","author":"Eikmanns","year":"1992","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.ymben.2020.01.004_bib15","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/1475-2859-6-27","article-title":"Comparison of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum","volume":"6","author":"Faijes","year":"2007","journal-title":"Microb. Cell Factories"},{"key":"10.1016\/j.ymben.2020.01.004_bib16","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1128\/AEM.07056-11","article-title":"Improvement of the redox balance increases L-valine production by Corynebacterium glutamicum under oxygen deprivation conditions","volume":"78","author":"Hasegawa","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ymben.2020.01.004_bib17","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.1128\/AEM.02806-12","article-title":"Engineering of Corynebacterium glutamicum for high-yield L-valine production under oxygen deprivation conditions","volume":"79","author":"Hasegawa","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ymben.2020.01.004_bib18","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.02638-16","article-title":"Enhanced glucose consumption and organic acid production by engineered Corynebacterium glutamicum based on analysis of a pfkB1 deletion mutant","volume":"83","author":"Hasegawa","year":"2017","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ymben.2020.01.004_bib19","doi-asserted-by":"crossref","first-page":"2259","DOI":"10.1128\/AEM.02625-07","article-title":"The Ehrlich pathway for fusel alcohol production: a century of research on Saccharomyces cerevisiae metabolism","volume":"74","author":"Hazelwood","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ymben.2020.01.004_bib20","first-page":"243","article-title":"Metabolic engineering of Corynebacterium glutamicum for fuel ethanol production under oxygen-deprivation conditions","volume":"8","author":"Inui","year":"2004","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2020.01.004_bib21","first-page":"182","article-title":"Metabolic analysis of Corynebacterium glutamicum during lactate and succinate productions under oxygen deprivation conditions","volume":"7","author":"Inui","year":"2004","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2020.01.004_bib22","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1111\/j.1574-6968.1993.tb06383.x","article-title":"Characterization of phosphoenolpyruvate carboxykinase fromCorynebacterium glutamicum","volume":"111","author":"Jetten","year":"1993","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.ymben.2020.01.004_bib23","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s00253-010-2493-7","article-title":"Engineering of sugar metabolism of Corynebacterium glutamicum for production of amino acid L-alanine under oxygen deprivation","volume":"87","author":"Jojima","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2020.01.004_bib24","doi-asserted-by":"crossref","first-page":"4228","DOI":"10.1016\/j.febslet.2012.10.028","article-title":"Identification of a HAD superfamily phosphatase, HdpA, involved in 1,3-dihydroxyacetone production during sugar catabolism in Corynebacterium glutamicum","volume":"586","author":"Jojima","year":"2012","journal-title":"FEBS Lett."},{"key":"10.1016\/j.ymben.2020.01.004_bib25","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1007\/s00253-014-6223-4","article-title":"Metabolic engineering for improved production of ethanol by Corynebacterium glutamicum","volume":"99","author":"Jojima","year":"2015","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2020.01.004_bib26","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/S1389-1723(01)80308-X","article-title":"Bacterial phosphotransferase system (PTS) in carbohydrate uptake and control of carbon metabolism","volume":"92","author":"Kotrba","year":"2001","journal-title":"J. Biosci. Bioeng."},{"key":"10.1016\/j.ymben.2020.01.004_bib27","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1007\/s00253-007-1094-6","article-title":"Transcriptionally regulated adhA gene encodes alcohol dehydrogenase required for ethanol and n-propanol utilization in Corynebacterium glutamicum R","volume":"76","author":"Kotrbova-Kozak","year":"2007","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2020.01.004_bib28","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/S0168-1656(03)00162-7","article-title":"Characterisation of the enzyme activities involved in the valine biosynthetic pathway in a valine-producing strain of Corynebacterium glutamicum","volume":"104","author":"Leyval","year":"2003","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.ymben.2020.01.004_bib29","doi-asserted-by":"crossref","first-page":"13066","DOI":"10.1074\/jbc.M113.536748","article-title":"Mycobacterium tuberculosis phosphoenolpyruvate carboxykinase is regulated by redox mechanisms and interaction with thioredoxin","volume":"289","author":"Machov\u00e1","year":"2014","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ymben.2020.01.004_bib30","doi-asserted-by":"crossref","first-page":"7457","DOI":"10.1128\/AEM.01025-08","article-title":"Metabolic function of Corynebacterium glutamicum aminotransferases AlaT and AvtA and impact on L-valine production","volume":"74","author":"Marienhagen","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ymben.2020.01.004_bib31","doi-asserted-by":"crossref","first-page":"7639","DOI":"10.1128\/JB.187.22.7639-7646.2005","article-title":"Functional analysis of all aminotransferase proteins inferred from the genome sequence of Corynebacterium glutamicum","volume":"187","author":"Marienhagen","year":"2005","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.ymben.2020.01.004_bib32","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/1475-2859-12-119","article-title":"Increased isobutanol production in Saccharomyces cerevisiae by eliminating competing pathways and resolving cofactor imbalance","volume":"12","author":"Matsuda","year":"2013","journal-title":"Microb. Cell Factories"},{"key":"10.1016\/j.ymben.2020.01.004_bib33","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.jbiosc.2017.04.005","article-title":"Heterologous expression of bacterial phosphoenol pyruvate carboxylase and Entner-Doudoroff pathway in Saccharomyces cerevisiae for improvement of isobutanol production","volume":"124","author":"Morita","year":"2017","journal-title":"J. Biosci. Bioeng."},{"key":"10.1016\/j.ymben.2020.01.004_bib34","doi-asserted-by":"crossref","first-page":"3442","DOI":"10.1046\/j.1432-1327.2000.01354.x","article-title":"Kinetic properties of the glucose-6-phosphate and 6-phosphogluconate dehydrogenases from Corynebacterium glutamicum and their application for predicting pentose phosphate pathway flux in vivo","volume":"267","author":"Moritz","year":"2000","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.ymben.2020.01.004_bib35","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1007\/s00253-007-1336-7","article-title":"Production of D-lactic acid by Corynebacterium glutamicum under oxygen deprivation","volume":"78","author":"Okino","year":"2008","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2020.01.004_bib36","first-page":"91","article-title":"Corynebacterium glutamicum glyceraldehyde-3-phosphate dehydrogenase isoforms with opposite, ATP-dependent regulation","volume":"8","author":"Omumasaba","year":"2004","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2020.01.004_bib37","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1099\/00221287-144-4-915","article-title":"Pyruvate carboxylase from Corynebacterium glutamicum: characterization, expression and inactivation of the pyc gene","volume":"144","author":"Peters-Wendisch","year":"1998","journal-title":"Microbiology"},{"key":"10.1016\/j.ymben.2020.01.004_bib38","article-title":"Model-driven redox pathway manipulation for improved isobutanol production in Bacillus subtilis complemented with experimental validation and metabolic profiling analysis","volume":"9","author":"Qi","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.ymben.2020.01.004_bib39","first-page":"573","article-title":"Characterization of the phosphoenolpyruvate carboxykinase gene from Corynebacterium glutamicum and significance of the enzyme for growth and amino acid production","volume":"3","author":"Riedel","year":"2001","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2020.01.004_bib40","first-page":"37","article-title":"Ethanol production by Zymomonas mobilis","volume":"23","author":"Rogers","year":"1982","journal-title":"Adv. Biochem. Eng."},{"key":"10.1016\/j.ymben.2020.01.004_bib41","series-title":"Molecular Cloning: a Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"10.1016\/j.ymben.2020.01.004_bib42","doi-asserted-by":"crossref","first-page":"6613","DOI":"10.1074\/jbc.M311657200","article-title":"The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli","volume":"279","author":"Sauer","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ymben.2020.01.004_bib43","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1042\/bj3260731","article-title":"Allosteric control of Zymomonas mobilis glucose-6-phosphate dehydrogenase by phosphoenolpyruvate","volume":"326","author":"Scopes","year":"1997","journal-title":"Biochem. J."},{"key":"10.1016\/j.ymben.2020.01.004_bib44","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1007\/s00253-010-2522-6","article-title":"Engineering Corynebacterium glutamicum for isobutanol production","volume":"87","author":"Smith","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2020.01.004_bib45","doi-asserted-by":"crossref","first-page":"6693","DOI":"10.1007\/s00253-013-4986-7","article-title":"Reactions upstream of glycerate-1,3-bisphosphate drive Corynebacterium glutamicum D-lactate productivity under oxygen deprivation","volume":"97","author":"Tsuge","year":"2013","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"Suppl. 4","key":"10.1016\/j.ymben.2020.01.004_bib46","first-page":"S467","article-title":"You're one in a googol: optimizing genes for protein expression","volume":"6","author":"Welch","year":"2009","journal-title":"J. R. Soc. Interface"},{"key":"10.1016\/j.ymben.2020.01.004_bib47","doi-asserted-by":"crossref","first-page":"4447","DOI":"10.1128\/AEM.07998-11","article-title":"Overexpression of genes encoding glycolytic enzymes in Corynebacterium glutamicum enhances glucose metabolism and alanine production under oxygen deprivation conditions","volume":"78","author":"Yamamoto","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ymben.2020.01.004_bib48","doi-asserted-by":"crossref","first-page":"2938","DOI":"10.1002\/bit.24961","article-title":"Strain optimization for efficient isobutanol production using Corynebacterium glutamicum under oxygen deprivation","volume":"110","author":"Yamamoto","year":"2013","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.ymben.2020.01.004_bib49","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1099\/mic.0.2006\/003657-0","article-title":"Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R","volume":"153","author":"Yukawa","year":"2007","journal-title":"Microbiology"},{"key":"10.1016\/j.ymben.2020.01.004_bib50","doi-asserted-by":"crossref","first-page":"20653","DOI":"10.1073\/pnas.0807157106","article-title":"Expanding metabolism for biosynthesis of nonnatural alcohols","volume":"105","author":"Zhang","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U. S. A"}],"container-title":["Metabolic Engineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096717619303453?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096717619303453?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T23:01:40Z","timestamp":1761087700000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1096717619303453"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5]]},"references-count":50,"alternative-id":["S1096717619303453"],"URL":"https:\/\/doi.org\/10.1016\/j.ymben.2020.01.004","relation":{},"ISSN":["1096-7176"],"issn-type":[{"value":"1096-7176","type":"print"}],"subject":[],"published":{"date-parts":[[2020,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Isobutanol production in Corynebacterium glutamicum: Suppressed succinate by-production by pckA inactivation and enhanced productivity via the Entner\u2013Doudoroff pathway","name":"articletitle","label":"Article Title"},{"value":"Metabolic Engineering","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymben.2020.01.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}