{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T13:16:40Z","timestamp":1778591800414,"version":"3.51.4"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["FKZ INST 256\/418-1"],"award-info":[{"award-number":["FKZ INST 256\/418-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["FKZ 031B034"],"award-info":[{"award-number":["FKZ 031B034"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Metabolic Engineering"],"published-print":{"date-parts":[[2021,9]]},"DOI":"10.1016\/j.ymben.2021.05.004","type":"journal-article","created":{"date-parts":[[2021,5,26]],"date-time":"2021-05-26T22:15:56Z","timestamp":1622067356000},"page":"11-18","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"special_numbering":"C","title":["Engineering the precursor pool to modulate the production of pamamycins in the heterologous host S. albus J1074"],"prefix":"10.1016","volume":"67","author":[{"given":"Nils","family":"Gummerlich","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niko","family":"Manderscheid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5071-8430","authenticated-orcid":false,"given":"Yuriy","family":"Rebets","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maksym","family":"Myronovskyi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Gl\u00e4ser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Kuhl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7952-985X","authenticated-orcid":false,"given":"Christoph","family":"Wittmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andriy","family":"Luzhetskyy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymben.2021.05.004_bib1","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.ymben.2021.05.004_bib2","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1039\/B801658P","article-title":"Biosynthesis of polyketide synthase extender units","volume":"26","author":"Chan","year":"2009","journal-title":"Nat. Prod. Rep."},{"key":"10.1016\/j.ymben.2021.05.004_bib3","doi-asserted-by":"crossref","first-page":"31228","DOI":"10.1074\/jbc.M203263200","article-title":"Kinetic and structural analysis of a new group of Acyl-CoA carboxylases found in Streptomyces coelicolor A3(2)","volume":"277","author":"Diacovich","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ymben.2021.05.004_bib4","doi-asserted-by":"crossref","first-page":"2441","DOI":"10.1039\/C4MB00287C","article-title":"KEGG-PATH: kyoto encyclopedia of genes and genomes-based pathway analysis using a path analysis model","volume":"10","author":"Du","year":"2014","journal-title":"Mol. Biosyst."},{"key":"10.1016\/j.ymben.2021.05.004_bib5","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1111\/j.1574-6968.1997.tb13882.x","article-title":"High efficiency intergeneric conjugal transfer of plasmid DNA from Escherichia coli to methyl DNA-restricting streptomycetes","volume":"155","author":"Flett","year":"1997","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.ymben.2021.05.004_bib6","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1186\/s12934-020-01413-1","article-title":"A common approach for absolute quantification of short chain CoA thioesters in prokaryotic and eukaryotic microbes","volume":"19","author":"Gl\u00e4ser","year":"2020","journal-title":"Microb. Cell Factories"},{"key":"10.1016\/j.ymben.2021.05.004_bib7","series-title":"Molecular Cloning : a Laboratory Manual","author":"Green","year":"2012"},{"key":"10.1016\/j.ymben.2021.05.004_bib8","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0065-2164(04)54004-2","article-title":"Lambda red-mediated genetic manipulation of antibiotic-producing Streptomyces","volume":"54","author":"Gust","year":"2004","journal-title":"Adv. Appl. Microbiol."},{"key":"10.1016\/j.ymben.2021.05.004_bib9","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1093\/emboj\/cdg244","article-title":"Transcarboxylase 12S crystal structure: hexamer assembly and substrate binding to a multienzyme core","volume":"22","author":"Hall","year":"2003","journal-title":"EMBO J."},{"key":"10.1016\/j.ymben.2021.05.004_bib10","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.7164\/antibiotics.51.1040","article-title":"Discovery of new homologous pamamycins by mass spectrometry and post mortem inhibitory action on autolysis of chicken embryo chorioallantoic membrane blood vessels","volume":"51","author":"Hartl","year":"1998","journal-title":"J. Antibiot. (Tokyo)"},{"key":"10.1016\/j.ymben.2021.05.004_bib11","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1039\/C8NP00039E","article-title":"The architectures of iterative type I PKS and FAS","volume":"35","author":"Herbst","year":"2018","journal-title":"Nat. Prod. Rep."},{"key":"10.1016\/j.ymben.2021.05.004_bib12","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/s10295-015-1723-5","article-title":"Natural product discovery: past, present, and future","volume":"43","author":"Katz","year":"2016","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2021.05.004_bib13","series-title":"Practical Streptomyces Genetics","author":"Kieser","year":"2010"},{"key":"10.1016\/j.ymben.2021.05.004_bib14","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.7164\/antibiotics.41.1196","article-title":"Isolation, physico-chemical properties and biological activity of pamamycin-607, an aerial mycelium-inducing substance from Streptomyces alboniger","volume":"41","author":"Kondo","year":"1988","journal-title":"J. Antibiot. (Tokyo)"},{"key":"10.1016\/j.ymben.2021.05.004_bib15","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1039\/C9MD00141G","article-title":"Diversification of polyketide structures via synthase engineering","volume":"10","author":"Kornfuehrer","year":"2019","journal-title":"MedChemComm"},{"key":"10.1016\/j.ymben.2021.05.004_bib16","doi-asserted-by":"crossref","first-page":"3858","DOI":"10.1002\/bit.27537","article-title":"Microparticles globally reprogram Streptomyces albus toward accelerated morphogenesis, streamlined carbon core metabolism, and enhanced production of the antituberculosis polyketide pamamycin","volume":"117","author":"Kuhl","year":"2020","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.ymben.2021.05.004_bib17","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1006\/mben.2000.0169","article-title":"Precursor supply for polyketide biosynthesis: the role of crotonyl-CoA reductase","volume":"3","author":"Liu","year":"2001","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2021.05.004_bib18","doi-asserted-by":"crossref","first-page":"673","DOI":"10.7164\/antibiotics.32.673","article-title":"Pamamycin: a new antibiotic and stimulator of aerial mycelia formation","volume":"32","author":"McCann","year":"1979","journal-title":"J. Antibiot. (Tokyo)"},{"key":"10.1016\/j.ymben.2021.05.004_bib19","doi-asserted-by":"crossref","first-page":"4557","DOI":"10.1007\/s00253-014-5523-z","article-title":"Iterative marker excision system","volume":"98","author":"Myronovskyi","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2021.05.004_bib20","doi-asserted-by":"crossref","first-page":"5370","DOI":"10.1128\/AEM.00434-11","article-title":"Beta-glucuronidase as a sensitive and versatile reporter in actinomycetes","volume":"77","author":"Myronovskyi","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ymben.2021.05.004_bib21","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.7164\/antibiotics.48.1159","article-title":"Structure-activity relationship of pamamycins: effects of alkyl substituents","volume":"48","author":"Natsume","year":"1995","journal-title":"J. Antibiot. (Tokyo)"},{"key":"10.1016\/j.ymben.2021.05.004_bib22","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1021\/acs.jnatprod.9b01285","article-title":"Natural products as sources of new drugs over the nearly four decades from 01\/1981 to 09\/2019","volume":"83","author":"Newman","year":"2020","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.ymben.2021.05.004_bib23","doi-asserted-by":"crossref","first-page":"2280","DOI":"10.1002\/anie.201408901","article-title":"Insights into the pamamycin biosynthesis","volume":"54","author":"Rebets","year":"2015","journal-title":"Angew Chem. Int. Ed. Engl."},{"key":"10.1016\/j.ymben.2021.05.004_bib24","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1007\/s10295-006-0094-3","article-title":"Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea","volume":"33","author":"Reeves","year":"2006","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2021.05.004_bib25","doi-asserted-by":"crossref","first-page":"4166","DOI":"10.1128\/AEM.67.9.4166-4176.2001","article-title":"Role of an essential acyl coenzyme A carboxylase in the primary and secondary metabolism of Streptomyces coelicolor A3(2)","volume":"67","author":"Rodriguez","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"issue":"Pt 11","key":"10.1016\/j.ymben.2021.05.004_bib26","doi-asserted-by":"crossref","first-page":"3109","DOI":"10.1099\/00221287-145-11-3109","article-title":"Genetic and biochemical characterization of the alpha and beta components of a propionyl-CoA carboxylase complex of Streptomyces coelicolor A3(2)","volume":"145","author":"Rodriguez","year":"1999","journal-title":"Microbiology"},{"key":"10.1016\/j.ymben.2021.05.004_bib27","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1039\/a909079g","article-title":"Polyketide biosynthesis: a millennium review","volume":"18","author":"Staunton","year":"2001","journal-title":"Nat. Prod. Rep."},{"key":"10.1016\/j.ymben.2021.05.004_bib28","doi-asserted-by":"crossref","first-page":"2611","DOI":"10.1021\/cr9600316","article-title":"Biosynthesis of erythromycin and rapamycin","volume":"97","author":"Staunton","year":"1997","journal-title":"Chem. Rev."},{"key":"10.1016\/j.ymben.2021.05.004_bib29","doi-asserted-by":"crossref","first-page":"1784","DOI":"10.1007\/s00018-005-5121-4","article-title":"Acetyl-coenzyme A carboxylase: crucial metabolic enzyme and attractive target for drug discovery","volume":"62","author":"Tong","year":"2005","journal-title":"Cell. Mol. Life Sci."}],"container-title":["Metabolic Engineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S109671762100080X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S109671762100080X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T12:47:50Z","timestamp":1778590070000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S109671762100080X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9]]},"references-count":29,"alternative-id":["S109671762100080X"],"URL":"https:\/\/doi.org\/10.1016\/j.ymben.2021.05.004","relation":{},"ISSN":["1096-7176"],"issn-type":[{"value":"1096-7176","type":"print"}],"subject":[],"published":{"date-parts":[[2021,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Engineering the precursor pool to modulate the production of pamamycins in the heterologous host S. albus J1074","name":"articletitle","label":"Article Title"},{"value":"Metabolic Engineering","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymben.2021.05.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}