{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T20:18:46Z","timestamp":1792873126871,"version":"4.5.0"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Metabolic Engineering"],"published-print":{"date-parts":[[2022,9]]},"DOI":"10.1016\/j.ymben.2022.08.004","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T02:07:15Z","timestamp":1660702035000},"page":"225-234","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":28,"special_numbering":"C","title":["Dual \u03b2-oxidation pathway and transcription factor engineering for methyl ketones production in Saccharomyces cerevisiae"],"prefix":"10.1016","volume":"73","author":[{"given":"Ge","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinhong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongjie","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymben.2022.08.004_bib1","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1002\/yea.1365","article-title":"PCR-mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiae","volume":"23","author":"Akada","year":"2006","journal-title":"Yeast"},{"key":"10.1016\/j.ymben.2022.08.004_bib2","doi-asserted-by":"crossref","first-page":"22208","DOI":"10.1074\/jbc.274.32.22208","article-title":"Functional analysis of the Zn2Cys6 transcription factors Oaf1p and Pip2p: different roles in fatty acid induction of \u03b2-oxidation in Saccharomyces cerevisiae","volume":"274","author":"Baumgartner","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ymben.2022.08.004_bib3","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1046\/j.1365-2958.2001.02421.x","article-title":"Transcriptional control of the GAL\/MEL regulon of yeast Saccharomyces cerevisiae: mechanism of galactose-mediated signal transduction","volume":"40","author":"Bhat","year":"2001","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.ymben.2022.08.004_bib4","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/S0958-6946(03)00109-2","article-title":"Lipolysis and free fatty acid catabolism in cheese: a review of current knowledge","volume":"13","author":"Collins","year":"2003","journal-title":"Int. Dairy J."},{"key":"10.1016\/j.ymben.2022.08.004_bib5","first-page":"1507","article-title":"ADR1c mutations enhance the ability of ADR1 to activate transcription by a mechanism that is independent of effects on cyclic AMP-dependent protein-kinase phosphorylation of Ser-230","volume":"12","author":"Denis","year":"1992","journal-title":"Mol. Cell Biol."},{"key":"10.1016\/j.ymben.2022.08.004_bib6","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1002\/bit.26995","article-title":"Methyl ketone production by Pseudomonas putida is enhanced by plant-derived amino acids","volume":"116","author":"Dong","year":"2019","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.ymben.2022.08.004_bib7","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1083\/jcb.200711136","article-title":"Fld1p, a functional homologue of human seipin, regulates the size of lipid droplets in yeast","volume":"180","author":"Fei","year":"2008","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.ymben.2022.08.004_bib8","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1073\/pnas.1715282115","article-title":"Redirection of lipid flux toward phospholipids in yeast increases fatty acid turnover and secretion","volume":"115","author":"Ferreira","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ymben.2022.08.004_bib9","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0076-6879(02)50957-5","article-title":"Transformation of yeast by lithium acetate\/single-stranded carrier DNA\/polyethylene glycol method","volume":"350","author":"Gietz","year":"2002","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.ymben.2022.08.004_bib10","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1128\/AEM.06785-11","article-title":"Engineering of bacterial methyl ketone synthesis for biofuels","volume":"78","author":"Goh","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ymben.2022.08.004_bib11","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.ymben.2014.09.003","article-title":"Substantial improvements in methyl ketone production in E. coli and insights on the pathway from in vitro studies","volume":"26","author":"Goh","year":"2014","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2022.08.004_bib12","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1002\/bit.26558","article-title":"Improving methyl ketone production in Escherichia coli by heterologous expression of NADH-dependent FabG","volume":"115","author":"Goh","year":"2018","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.ymben.2022.08.004_bib13","doi-asserted-by":"crossref","DOI":"10.1093\/femsyr\/foy096","article-title":"Impact of forced fatty acid synthesis on metabolism and physiology of Saccharomyces cerevisiae","volume":"18","author":"Gossing","year":"2018","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.ymben.2022.08.004_bib14","doi-asserted-by":"crossref","first-page":"8597","DOI":"10.1073\/pnas.88.19.8597","article-title":"Regulated expression of the GAL4 activator gene in yeast provides a sensitive genetic switch for glucose repression","volume":"88","author":"Griggs","year":"1991","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ymben.2022.08.004_bib15","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1038\/nchembio.2429","article-title":"Harnessing yeast organelles for metabolic engineering","volume":"13","author":"Hammer","year":"2017","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.ymben.2022.08.004_bib16","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.ymben.2018.05.018","article-title":"Engineering \u03b2-oxidation in Yarrowia lipolytica for methyl ketone production","volume":"48","author":"Hanko","year":"2018","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2022.08.004_bib17","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1039\/C7SE00415J","article-title":"High cetane renewable diesel fuels prepared from bio-based methyl ketones and diols","volume":"2","author":"Harrison","year":"2018","journal-title":"Sustain. Energy Fuels"},{"key":"10.1016\/j.ymben.2022.08.004_bib18","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0168-6445(03)00017-2","article-title":"The biochemistry of peroxisomal \u03b2-oxidation in the yeast Saccharomyces cerevisiae","volume":"27","author":"Hiltunen","year":"2003","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.ymben.2022.08.004_bib19","doi-asserted-by":"crossref","DOI":"10.1098\/rsob.190049","article-title":"Engineering Saccharomyces cerevisiae cells for production of fatty acid-derived biofuels and chemicals","volume":"9","author":"Hu","year":"2019","journal-title":"Open Biol"},{"key":"10.1016\/j.ymben.2022.08.004_bib20","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1002\/biot.201600147","article-title":"EasyClone-MarkerFree: a vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9","volume":"11","author":"Jessop-Fabre","year":"2016","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.ymben.2022.08.004_bib21","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1007\/s00253-012-4125-x","article-title":"Fast and accurate preparation fatty acid methyl esters by microwave-assisted derivatization in the yeast Saccharomyces cerevisiae","volume":"94","author":"Khoomrung","year":"2012","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2022.08.004_bib22","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1111\/j.1365-2915.2010.00918.x","article-title":"First report of the repellency of 2-tridecanone against ticks","volume":"25","author":"Kimps","year":"2011","journal-title":"Med. Vet. Entomol."},{"key":"10.1016\/j.ymben.2022.08.004_bib23","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.ymben.2014.11.006","article-title":"Overproduction and secretion of free fatty acids through disrupted neutral lipid recycle in Saccharomyces cerevisiae","volume":"28","author":"Leber","year":"2015","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2022.08.004_bib24","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1021\/sb500243c","article-title":"Reversal of the \u03b2-oxidation cycle in Saccharomyces cerevisiae for production of fuels and chemicals","volume":"4","author":"Lian","year":"2015","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.ymben.2022.08.004_bib25","doi-asserted-by":"crossref","first-page":"fov004","DOI":"10.1093\/femsyr\/fov004","article-title":"CRISPR\/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae","volume":"15","author":"Mans","year":"2015","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.ymben.2022.08.004_bib26","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/978-981-13-2233-4_16","article-title":"Characterization of peroxisomal regulation networks","volume":"89","author":"Mast","year":"2018","journal-title":"Subcell. Biochem."},{"key":"10.1016\/j.ymben.2022.08.004_bib27","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.ceb.2015.05.002","article-title":"Signaling dynamics and peroxisomes","volume":"35","author":"Mast","year":"2015","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.ymben.2022.08.004_bib28","doi-asserted-by":"crossref","first-page":"4433","DOI":"10.1128\/AEM.00973-13","article-title":"Engineering of Ralstonia eutropha H16 for autotrophic and heterotrophic production of methyl ketones","volume":"79","author":"Muller","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ymben.2022.08.004_bib29","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1111\/j.1574-6976.1997.tb00352.x","article-title":"Osmoregulation and glycerol metabolism in the yeast Saccharomyces cerevisiae","volume":"21","author":"Nevoigt","year":"1997","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.ymben.2022.08.004_bib30","doi-asserted-by":"crossref","DOI":"10.1002\/biot.201800421","article-title":"Yeast systems biology: model organism and cell factory","volume":"14","author":"Nielsen","year":"2019","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.ymben.2022.08.004_bib31","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.ymben.2020.08.003","article-title":"High titer methyl ketone production with tailored Pseudomonas taiwanensis VLB120","volume":"62","author":"Nies","year":"2020","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2022.08.004_bib32","doi-asserted-by":"crossref","first-page":"8877","DOI":"10.1074\/jbc.M111646200","article-title":"The DGA1 gene determines a second triglyceride synthetic pathway in yeast","volume":"277","author":"Oelkers","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ymben.2022.08.004_bib33","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1007\/s10295-012-1178-x","article-title":"Synthesis of methyl ketones by metabolically engineered Escherichia coli","volume":"39","author":"Park","year":"2012","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2022.08.004_bib34","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1021\/acssynbio.7b00355","article-title":"An expanded heterologous GAL promoter collection for diauxie-inducible expression in Saccharomyces cerevisiae","volume":"7","author":"Peng","year":"2018","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.ymben.2022.08.004_bib35","doi-asserted-by":"crossref","first-page":"10703","DOI":"10.1074\/jbc.M109.079848","article-title":"Snf1 dependence of peroxisomal gene expression is mediated by Adr1","volume":"285","author":"Ratnakumar","year":"2010","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ymben.2022.08.004_bib36","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.bbalip.2006.07.004","article-title":"Fatty acid synthesis and elongation in yeast","volume":"1771","author":"Tehlivets","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ymben.2022.08.004_bib37","doi-asserted-by":"crossref","first-page":"1838","DOI":"10.1007\/s00018-003-3076-x","article-title":"Fatty acid metabolism in Saccharomyces cerevisiae","volume":"60","author":"van Roermund","year":"2003","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.ymben.2022.08.004_bib38","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1002\/yea.320030204","article-title":"Proliferation of microbodies in Saccharomyces cerevisiae","volume":"3","author":"Veenhuis","year":"1987","journal-title":"Yeast"},{"key":"10.1016\/j.ymben.2022.08.004_bib39","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1002\/yea.320080703","article-title":"Effect of benzoic acid on metabolic fluxes in yeasts: a continuous-culture study on the regulation of respiration and alcoholic fermentation","volume":"8","author":"Verduyn","year":"1992","journal-title":"Yeast"},{"key":"10.1016\/j.ymben.2022.08.004_bib40","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s12934-018-0862-6","article-title":"Engineering E. coli for simultaneous glucose-xylose utilization during methyl ketone production, Microb","volume":"17","author":"Wang","year":"2018","journal-title":"Cell Fact"},{"key":"10.1016\/j.ymben.2022.08.004_bib41","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.ymben.2019.04.009","article-title":"Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals","volume":"58","author":"Yan","year":"2020","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2022.08.004_bib42","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.ymben.2020.05.008","article-title":"Metabolic engineering of \u03b2-oxidation to leverage thioesterases for production of 2-heptanone, 2-nonanone and 2-undecanone","volume":"61","author":"Yan","year":"2020","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2022.08.004_bib43","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1104\/pp.110.157073","article-title":"Enzymatic functions of wild tomato methylketone synthases 1 and 2","volume":"154","author":"Yu","year":"2010","journal-title":"Plant Physiol."},{"key":"10.1016\/j.ymben.2022.08.004_bib44","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1016\/j.cell.2018.07.013","article-title":"Reprogramming yeast metabolism from alcoholic fermentation to lipogenesis","volume":"174","author":"Yu","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.ymben.2022.08.004_bib45","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms11709","article-title":"Production of fatty acid-derived oleochemicals and biofuels by synthetic yeast cell factories","volume":"7","author":"Zhou","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ymben.2022.08.004_bib46","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1002\/ps.4749","article-title":"Novel use of aliphatic n-methyl ketones as a fumigant and alternative to methyl bromide for insect control","volume":"74","author":"Zhu","year":"2018","journal-title":"Pest Manag. Sci."}],"container-title":["Metabolic Engineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096717622000994?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096717622000994?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T23:14:02Z","timestamp":1761088442000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1096717622000994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9]]},"references-count":46,"alternative-id":["S1096717622000994"],"URL":"https:\/\/doi.org\/10.1016\/j.ymben.2022.08.004","relation":{},"ISSN":["1096-7176"],"issn-type":[{"value":"1096-7176","type":"print"}],"subject":[],"published":{"date-parts":[[2022,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Dual \u03b2-oxidation pathway and transcription factor engineering for methyl ketones production in Saccharomyces cerevisiae","name":"articletitle","label":"Article Title"},{"value":"Metabolic Engineering","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymben.2022.08.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}