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Plant cell lines established from the medicinal plant Veratrum californicum. 20090305338 A1. https:\/\/patents.google.com\/patent\/US20090305338A1\/en. (Accessed 14 March 2024)."},{"issue":"7086","key":"10.1016\/j.ymben.2024.07.011_bib69","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1038\/nature04640","article-title":"Production of the antimalarial drug precursor artemisinic acid in engineered yeast","volume":"440","author":"Ro","year":"2006","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.ymben.2024.07.011_bib70","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12934-016-0447-1","article-title":"ATP citrate lyase mediated cytosolic acetyl-CoA biosynthesis increases mevalonate production in Saccharomyces cerevisiae","volume":"15","author":"Rodriguez","year":"2016","journal-title":"Microb. 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Eng."},{"key":"10.1016\/j.ymben.2024.07.011_bib77","article-title":"Transient expression in Nicotiana benthamiana leaves for triterpene production at a preparative scale","volume":"138","author":"Stephenson","year":"2018","journal-title":"JoVE"},{"issue":"4","key":"10.1016\/j.ymben.2024.07.011_bib79","first-page":"134","article-title":"Photosynthetic characteristics of Veratrum californicum in varied greenhouse environments","volume":"1","author":"Sun","year":"2013","journal-title":"J. Med. Active Plants"},{"key":"10.1016\/j.ymben.2024.07.011_bib80","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2021.681666","article-title":"Combined biosynthetic pathway engineering and storage pool expansion for high-level production of ergosterol in industrial Saccharomyces cerevisiae","volume":"9","author":"Sun","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.ymben.2024.07.011_bib82","unstructured":"Tas, S., Avci, O., 2009. Use of Cyclopamine in the Treatment of Psoriasis. 7605167. https:\/\/patents.google.com\/patent\/AU2003230541B2\/en?oq=AU-2003230541-B2. (Accessed 14 March 2024)."},{"issue":"3","key":"10.1016\/j.ymben.2024.07.011_bib83","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1007\/BF00574208","article-title":"Alkaloids ofZygadenus sibiricus. The structure of verazinine","volume":"21","author":"Taskhanova","year":"1985","journal-title":"Chem. Nat. Compo."},{"issue":"14","key":"10.1016\/j.ymben.2024.07.011_bib84","doi-asserted-by":"crossref","first-page":"4400","DOI":"10.1021\/jm900305z","article-title":"Discovery of a potent and orally active Hedgehog pathway antagonist (IPI-926)","volume":"52","author":"Tremblay","year":"2009","journal-title":"J. Med. 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