{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T19:50:11Z","timestamp":1782762611508,"version":"3.54.5"},"reference-count":79,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T00:00:00Z","timestamp":1743120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/R01602\\u00D7\/1"],"award-info":[{"award-number":["BB\/R01602\\u00D7\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/T013176\/1"],"award-info":[{"award-number":["BB\/T013176\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/T011408\/1 - 19-ERACoBioTech- 33 SyCoLim"],"award-info":[{"award-number":["BB\/T011408\/1 - 19-ERACoBioTech- 33 SyCoLim"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000308","name":"British Council","doi-asserted-by":"publisher","award":["NAF\\\\R1\\\\201187"],"award-info":[{"award-number":["NAF\\\\R1\\\\201187"]}],"id":[{"id":"10.13039\/501100000308","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000308","name":"British Council","doi-asserted-by":"publisher","award":["18229"],"award-info":[{"award-number":["18229"]}],"id":[{"id":"10.13039\/501100000308","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000308","name":"British Council","doi-asserted-by":"publisher","award":["527429894"],"award-info":[{"award-number":["527429894"]}],"id":[{"id":"10.13039\/501100000308","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["DEUSBIO - 949080"],"award-info":[{"award-number":["DEUSBIO - 949080"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["101022370"],"award-info":[{"award-number":["101022370"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007601","name":"Horizon 2020","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007601","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001779","name":"Monash University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001779","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018627","name":"Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China","doi-asserted-by":"publisher","award":["ZR2020QC007"],"award-info":[{"award-number":["ZR2020QC007"]}],"id":[{"id":"10.13039\/501100018627","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Metabolic Engineering"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.ymben.2025.03.014","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T14:08:50Z","timestamp":1743430130000},"page":"30-43","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"special_numbering":"C","title":["Insights into the methanol utilization capacity of Y. lipolytica and improvements through metabolic engineering"],"prefix":"10.1016","volume":"91","author":[{"given":"Wei","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Newell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas","family":"Coppens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khushboo","family":"Borah Slater","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huadong","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Bell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Haritos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2631-5898","authenticated-orcid":false,"given":"Rodrigo","family":"Ledesma-Amaro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymben.2025.03.014_bib1","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.ymben.2018.07.016","article-title":"Metabolic engineering in the host Yarrowia lipolytica","volume":"50","author":"Abdel-Mawgoud","year":"2018","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2025.03.014_bib2","first-page":"E9271","volume":"vol. 115","author":"Bang","year":"2018"},{"key":"10.1016\/j.ymben.2025.03.014_bib3","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1006\/mben.2002.0227","article-title":"Metabolic engineering of Escherichia coli: increase of NADH availability by overexpressing an NAD+-dependent formate dehydrogenase","volume":"4","author":"Berr\u0131os-Rivera","year":"2002","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2025.03.014_bib4","series-title":"Methanol: the Basic Chemical and Energy Feedstock of the Future","author":"Bertau","year":"2014"},{"key":"10.1016\/j.ymben.2025.03.014_bib5","doi-asserted-by":"crossref","DOI":"10.15252\/msb.202110280","article-title":"Metabolic fluxes for nutritional flexibility of Mycobacterium tuberculosis","volume":"17","author":"Borah","year":"2021","journal-title":"Mol. Syst. Biol."},{"key":"10.1016\/j.ymben.2025.03.014_bib6","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1111\/1751-7915.12605","article-title":"Golden Gate Assembly system dedicated to complex pathway manipulation in Yarrowia lipolytica","volume":"10","author":"Celi\u0144ska","year":"2017","journal-title":"Microb. Biotechnol."},{"key":"10.1016\/j.ymben.2025.03.014_bib7","series-title":"Environmental Impacts of Sugar Production the Cultivation and Processing of Sugarcane and Sugar Beet","author":"Cheesman","year":"2004"},{"key":"10.1016\/j.ymben.2025.03.014_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.cell.2020.07.010","article-title":"Converting Escherichia coli to a synthetic methylotroph growing solely on methanol","author":"Chen","year":"2020","journal-title":"Cell"},{"key":"10.1016\/j.ymben.2025.03.014_bib9","doi-asserted-by":"crossref","DOI":"10.1002\/biot.202400290","article-title":"Unlocking the formate utilization of wild\u2010type Yarrowia lipolytica through adaptive laboratory evolution","volume":"19","author":"Chen","year":"2024","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.ymben.2025.03.014_bib10","doi-asserted-by":"crossref","first-page":"330","DOI":"10.3390\/biom10020330","article-title":"NAD+ metabolism and regulation: lessons from yeast","volume":"10","author":"Croft","year":"2020","journal-title":"Biomolecules"},{"key":"10.1016\/j.ymben.2025.03.014_bib11","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/0141-0229(87)90053-6","article-title":"Ethanol tolerance of yeast","volume":"9","author":"D'amore","year":"1987","journal-title":"Enzym. Microb. Technol."},{"key":"10.1016\/j.ymben.2025.03.014_bib12","series-title":"Methanol","first-page":"3","article-title":"Chapter 1 - methanol production and applications: an overview","author":"Dalena","year":"2018"},{"key":"10.1016\/j.ymben.2025.03.014_bib13","series-title":"Methanol","first-page":"3","article-title":"Methanol production and applications: an overview","author":"Dalena","year":"2018"},{"key":"10.1016\/j.ymben.2025.03.014_bib14","doi-asserted-by":"crossref","first-page":"3525","DOI":"10.1007\/s10311-022-01485-y","article-title":"Methanol fuel production, utilization, and techno-economy: a review","volume":"20","author":"Deka","year":"2022","journal-title":"Environ. Chem. Lett."},{"key":"10.1016\/j.ymben.2025.03.014_bib15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/ncomms11152","article-title":"Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways","volume":"7","author":"DeLoache","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ymben.2025.03.014_bib16","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1002\/cssc.201501013","article-title":"Research progress on the indirect hydrogenation of carbon dioxide to methanol","volume":"9","author":"Du","year":"2016","journal-title":"ChemSusChem"},{"key":"10.1016\/j.ymben.2025.03.014_bib17","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1002\/cssc.201501013","article-title":"Research progress on the indirect hydrogenation of carbon dioxide to methanol","volume":"9","author":"Du","year":"2016","journal-title":"ChemSusChem"},{"key":"10.1016\/j.ymben.2025.03.014_bib18","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/nature02579","article-title":"Genome evolution in yeasts","volume":"430","author":"Dujon","year":"2004","journal-title":"Nature"},{"key":"10.1016\/j.ymben.2025.03.014_bib19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1752-0509-7-74","article-title":"COBRApy: constraints-based reconstruction and analysis for python","volume":"7","author":"Ebrahim","year":"2013","journal-title":"BMC Syst. Biol."},{"key":"10.1016\/j.ymben.2025.03.014_bib20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-020-19390-9","article-title":"Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae","volume":"11","author":"Espinosa","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ymben.2025.03.014_bib21","article-title":"Native and synthetic methanol assimilation in Saccharomyces cerevisiae","author":"Espinosa","year":"2019","journal-title":"bioRxiv"},{"key":"10.1016\/j.ymben.2025.03.014_bib22","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.femsyr.2004.09.004","article-title":"Hydrophobic substrate utilisation by the yeast Yarrowia lipolytica, and its potential applications","volume":"5","author":"Fickers","year":"2005","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.ymben.2025.03.014_bib23","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/j.mimet.2003.07.003","article-title":"New disruption cassettes for rapid gene disruption and marker rescue in the yeast Yarrowia lipolytica","volume":"55","author":"Fickers","year":"2003","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.ymben.2025.03.014_bib24","first-page":"1","article-title":"Sugarcane biorefineries wastewater: bioremediation technologies for environmental sustainability","volume":"6","author":"Fito","year":"2019","journal-title":"Chem. Biol. Technol. Agriculture"},{"key":"10.1016\/j.ymben.2025.03.014_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.mec.2020.e00146","article-title":"De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica","volume":"11","author":"He","year":"2020","journal-title":"Metab. Eng. Commun."},{"key":"10.1016\/j.ymben.2025.03.014_bib26","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.ymben.2005.12.003","article-title":"Cofactor engineering in Saccharomyces cerevisiae: expression of a H2O-forming NADH oxidase and impact on redox metabolism","volume":"8","author":"Heux","year":"2006","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2025.03.014_bib27","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1128\/AEM.02040-09","article-title":"Metabolic impact of increased NADH availability in Saccharomyces cerevisiae","volume":"76","author":"Hou","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ymben.2025.03.014_bib28","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/S0065-2911(08)60294-5","article-title":"Effects of alcohols on micro-organisms","volume":"25","author":"Ingram","year":"1985","journal-title":"Adv. Microb. Physiol."},{"key":"10.1016\/j.ymben.2025.03.014_bib29","article-title":"Metabolic engineering of yeast and plants for the production of the biologically active hydroxystilbene","volume":"2012","author":"Jeandet","year":"2012","journal-title":"resveratrol. J. Biomed. Biotechnol."},{"key":"10.1016\/j.ymben.2025.03.014_bib30","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/s41589-021-00836-0","article-title":"Metabolic engineering strategies to enable microbial utilization of C1 feedstocks","volume":"17","author":"Jiang","year":"2021","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.ymben.2025.03.014_bib31","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1128\/aem.53.5.1196-1198.1987","article-title":"Adaptation of yeast cell membranes to ethanol","volume":"53","author":"Jimenez","year":"1987","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ymben.2025.03.014_bib32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-020-19235-5","article-title":"Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle","volume":"11","author":"Keller","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ymben.2025.03.014_bib33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/npjsba.2016.5","article-title":"Regulation of amino-acid metabolism controls flux to lipid accumulation in Yarrowia lipolytica","volume":"2","author":"Kerkhoven","year":"2016","journal-title":"npj Syst. Biol. Appl."},{"key":"10.1016\/j.ymben.2025.03.014_bib34","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1038\/s41929-019-0255-1","article-title":"Biological conversion of methane to methanol through genetic reassembly of native catalytic domains","volume":"2","author":"Kim","year":"2019","journal-title":"Nat. Catal."},{"key":"10.1016\/j.ymben.2025.03.014_bib35","first-page":"1","article-title":"Growth of E. coli on formate and methanol via the reductive glycine pathway","author":"Kim","year":"2020","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.ymben.2025.03.014_bib36","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1007\/BF00326302","article-title":"Multiple-copy integration in the yeast Yarrowia lipolytica","volume":"26","author":"Le Dall","year":"1994","journal-title":"Curr. Genet."},{"key":"10.1016\/j.ymben.2025.03.014_bib37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13068-015-0335-7","article-title":"Engineering Yarrowia lipolytica to produce biodiesel from raw starch","volume":"8","author":"Ledesma-Amaro","year":"2015","journal-title":"Biotechnol. Biofuels"},{"key":"10.1016\/j.ymben.2025.03.014_bib38","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.ymben.2016.07.001","article-title":"Metabolic engineering of Yarrowia lipolytica to produce chemicals and fuels from xylose","volume":"38","author":"Ledesma-Amaro","year":"2016","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2025.03.014_bib39","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1016\/j.tibtech.2016.04.010","article-title":"Metabolic engineering for expanding the substrate range of Yarrowia lipolytica","volume":"34","author":"Ledesma-Amaro","year":"2016","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.ymben.2025.03.014_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.mec.2019.e00118","article-title":"Modular engineering for microbial production of carotenoids","volume":"10","author":"Li","year":"2020","journal-title":"Metab. Eng. Commun."},{"key":"10.1016\/j.ymben.2025.03.014_bib41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ymben.2015.08.007","article-title":"De novo production of resveratrol from glucose or ethanol by engineered Saccharomyces cerevisiae","volume":"32","author":"Li","year":"2015","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2025.03.014_bib42","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1038\/s41929-021-00670-6","article-title":"Metabolic network remodelling enhances yeast's fitness on xylose using aerobic glycolysis","volume":"4","author":"Li","year":"2021","journal-title":"Nat. Catal."},{"key":"10.1016\/j.ymben.2025.03.014_bib43","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.ymben.2016.06.006","article-title":"13C Metabolic Flux Analysis of acetate conversion to lipids by Yarrowia lipolytica","volume":"38","author":"Liu","year":"2016","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2025.03.014_bib44","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2021.125877","article-title":"Engineering Yarrowia lipolytica to produce advanced biofuels: current status and perspectives","volume":"341","author":"Lu","year":"2021","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.ymben.2025.03.014_bib45","first-page":"1","article-title":"Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design","volume":"10","author":"Lu","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ymben.2025.03.014_bib46","first-page":"1","article-title":"Removal of lycopene substrate inhibition enables high carotenoid productivity in Yarrowia lipolytica","volume":"13","author":"Ma","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ymben.2025.03.014_bib47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-018-03937-y","article-title":"Methanol-essential growth of Escherichia coli","volume":"9","author":"Meyer","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ymben.2025.03.014_bib48","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.ymben.2014.12.008","article-title":"Engineering Escherichia coli for methanol conversion","volume":"28","author":"M\u00fcller","year":"2015","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2025.03.014_bib49","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13068-018-1010-6","article-title":"Engineering Yarrowia lipolytica to enhance lipid production from lignocellulosic materials","volume":"11","author":"Niehus","year":"2018","journal-title":"Biotechnol. Biofuels"},{"key":"10.1016\/j.ymben.2025.03.014_bib50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13068-019-1580-y","article-title":"Engineering the oleaginous yeast Yarrowia lipolytica to produce limonene from waste cooking oil","volume":"12","author":"Pang","year":"2019","journal-title":"Biotechnol. Biofuels"},{"key":"10.1016\/j.ymben.2025.03.014_bib51","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1002\/lite.200900017","article-title":"Biotechnological valorization of biodiesel derived glycerol waste through production of single cell oil and citric acid by Yarrowia lipolytica","volume":"21","author":"Papanikolaou","year":"2009","journal-title":"Lipid Technol."},{"key":"10.1016\/j.ymben.2025.03.014_bib52","article-title":"What makes Yarrowia lipolytica well suited for industry?","author":"Park","year":"2022","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.ymben.2025.03.014_bib53","doi-asserted-by":"crossref","first-page":"12691","DOI":"10.1073\/pnas.1601797113","article-title":"Scaffoldless engineered enzyme assembly for enhanced methanol utilization","volume":"113","author":"Price","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ymben.2025.03.014_bib54","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1021\/acssynbio.8b00481","article-title":"Phage-assisted evolution of Bacillus methanolicus methanol dehydrogenase 2","volume":"8","author":"Roth","year":"2019","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.ymben.2025.03.014_bib55","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12915-015-0186-5","article-title":"Systems-level organization of yeast methylotrophic lifestyle","volume":"13","author":"Ru\u00dfmayer","year":"2015","journal-title":"BMC Biol."},{"key":"10.1016\/j.ymben.2025.03.014_bib56","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.ymben.2020.08.009","article-title":"Engineering the oleaginous yeast Yarrowia lipolytica for high-level resveratrol production","volume":"62","author":"S\u00e1ez-S\u00e1ez","year":"2020","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2025.03.014_bib57","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.tibtech.2008.10.009","article-title":"Methanol-based industrial biotechnology: current status and future perspectives of methylotrophic bacteria","volume":"27","author":"Schrader","year":"2009","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.ymben.2025.03.014_bib58","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.coche.2015.08.004","article-title":"Manipulation of metabolism in complex eukaryotic systems to control cellular state","volume":"10","author":"Su","year":"2015","journal-title":"Curr. Opin. Chem. Eng."},{"key":"10.1016\/j.ymben.2025.03.014_bib59","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12934-017-0796-4","article-title":"Cofactor engineering to regulate NAD+\/NADH ratio with its application to phytosterols biotransformation","volume":"16","author":"Su","year":"2017","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.ymben.2025.03.014_bib60","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/BF00738541","article-title":"Plasma-membrane lipid composition and ethanol tolerance in Saccharomyces cerevisiae","volume":"117","author":"Thomas","year":"1978","journal-title":"Arch. Microbiol."},{"key":"10.1016\/j.ymben.2025.03.014_bib61","doi-asserted-by":"crossref","DOI":"10.1155\/2012\/378384","article-title":"Oil production from Yarrowia lipolytica Po1g using rice bran hydrolysate","volume":"2012","author":"Tsigie","year":"2012","journal-title":"J. Biomed. Biotechnol."},{"key":"10.1016\/j.ymben.2025.03.014_bib62","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.ymben.2018.07.011","article-title":"Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production","volume":"49","author":"Tuyishime","year":"2018","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2025.03.014_bib63","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1098\/rspb.1999.0817","article-title":"The origin of eukaryotes: the difference between prokaryotic and eukaryotic cells","volume":"266","author":"Vellai","year":"1999","journal-title":"Proc. R. Soc. Lond. Ser. B Biol. Sci."},{"key":"10.1016\/j.ymben.2025.03.014_bib64","doi-asserted-by":"crossref","DOI":"10.1021\/acssynbio.1c00464","article-title":"Engineering yeast Yarrowia lipolytica for methanol assimilation","author":"Wang","year":"2021","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.ymben.2025.03.014_bib65","doi-asserted-by":"crossref","DOI":"10.1016\/j.tibtech.2019.12.013","article-title":"Synthetic methylotrophy: a practical solution for methanol-based biomanufacturing","author":"Wang","year":"2020","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.ymben.2025.03.014_bib66","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.copbio.2015.01.007","article-title":"Synthetic methylotrophy: engineering the production of biofuels and chemicals based on the biology of aerobic methanol utilization","volume":"33","author":"Whitaker","year":"2015","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.ymben.2025.03.014_bib67","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1104\/pp.113.3.943","article-title":"Binding of the peroxisomal targeting sequence SKL is specified by a low-affinity site in Castor bean glyoxysomal membranes (A domain next to the SKL binds to a high-affinity site)","volume":"113","author":"Wolins","year":"1997","journal-title":"Plant Physiol."},{"key":"10.1016\/j.ymben.2025.03.014_bib68","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-018-04795-4","article-title":"Improving formaldehyde consumption drives methanol assimilation in engineered E. coli","volume":"9","author":"Woolston","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ymben.2025.03.014_bib69","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.4014\/jmb.1412.12026","article-title":"Proteomic analysis of erythritol-producing Yarrowia lipolytica from glycerol in response to osmotic pressure","volume":"25","author":"Yang","year":"2015","journal-title":"J. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2025.03.014_bib70","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1007\/s12010-009-8684-y","article-title":"Toxicity of methanol and formaldehyde towards Saccharomyces cerevisiae as assessed by DNA microarray analysis","volume":"160","author":"Yasokawa","year":"2010","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.ymben.2025.03.014_bib71","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1093\/bioinformatics\/btu015","article-title":"INCA: a computational platform for isotopically non-stationary metabolic flux analysis","volume":"30","author":"Young","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ymben.2025.03.014_bib72","doi-asserted-by":"crossref","DOI":"10.1093\/femsyr\/foab009","article-title":"Beyond alcohol oxidase: the methylotrophic yeast Komagataella phaffii utilizes methanol also with its native alcohol dehydrogenase Adh2","volume":"21","author":"Zavec","year":"2021","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.ymben.2025.03.014_bib73","article-title":"The glyoxylate-serine pathway enables conversion of Saccharomyces cerevisiae to a synthetic methylotroph","author":"Zhan","year":"2021","journal-title":"Saccharomyces cerevisiae"},{"key":"10.1016\/j.ymben.2025.03.014_bib74","article-title":"The glyoxylate-serine pathway enables conversion of Saccharomyces cerevisiae to a synthetic methylotroph","author":"Zhan","year":"2021","journal-title":"Saccharomyces cerevisiae"},{"key":"10.1016\/j.ymben.2025.03.014_bib75","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1038\/s41929-023-00957-w","article-title":"Reprogramming methanol utilization pathways to convert Saccharomyces cerevisiae to a synthetic methylotroph","volume":"6","author":"Zhan","year":"2023","journal-title":"Nat. Catal."},{"key":"10.1016\/j.ymben.2025.03.014_bib76","doi-asserted-by":"crossref","DOI":"10.1038\/s41929-023-00957-w","article-title":"Reprogramming methanol utilization pathways to convert Saccharomyces cerevisiae to a synthetic methylotroph","author":"Zhan","year":"2023","journal-title":"Nat. Catal."},{"key":"10.1016\/j.ymben.2025.03.014_bib77","doi-asserted-by":"crossref","first-page":"3655","DOI":"10.1002\/bit.27862","article-title":"Strategies and challenges with the microbial conversion of methanol to high-value chemicals","volume":"118","author":"Zhan","year":"2021","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.ymben.2025.03.014_bib78","article-title":"Improving methanol assimilation in Yarrowia lipolytica via systematic metabolic engineering combined with compartmentalization","author":"Zhang","year":"2022","journal-title":"Green Chem."},{"key":"10.1016\/j.ymben.2025.03.014_bib79","doi-asserted-by":"crossref","first-page":"4083","DOI":"10.1039\/C6RA27038G","article-title":"Guidance for engineering of synthetic methylotrophy based on methanol metabolism in methylotrophy","volume":"7","author":"Zhang","year":"2017","journal-title":"RSC Adv."}],"updated-by":[{"DOI":"10.1016\/j.ymben.2025.04.004","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000}}],"container-title":["Metabolic Engineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096717625000503?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096717625000503?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T13:37:04Z","timestamp":1769089024000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1096717625000503"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":79,"alternative-id":["S1096717625000503"],"URL":"https:\/\/doi.org\/10.1016\/j.ymben.2025.03.014","relation":{},"ISSN":["1096-7176"],"issn-type":[{"value":"1096-7176","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Insights into the methanol utilization capacity of Y. lipolytica and improvements through metabolic engineering","name":"articletitle","label":"Article Title"},{"value":"Metabolic Engineering","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymben.2025.03.014","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Crown Copyright \u00a9 2025 Published by Elsevier Inc. on behalf of International Metabolic Engineering Society.","name":"copyright","label":"Copyright"}]}}