{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:00:28Z","timestamp":1785416428430,"version":"3.56.0"},"reference-count":58,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100008332","name":"TU Graz, Internationale Beziehungen und Mobilit\u00e4tsprogramme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008332","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013924","name":"Wirtschaftsagentur Wien","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013924","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012416","name":"Bundesministerium f\u00fcr Digitalisierung und Wirtschaftsstandort","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012416","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007945","name":"Steirische Wirtschaftsf\u00f6rderungsgesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007945","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011035","name":"Standortagentur Tirol","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011035","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Metabolic Engineering"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.ymben.2026.102487","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:24:08Z","timestamp":1781223848000},"page":"102487","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["MAPS: a marker-free genome integration system for Komagataella phaffii enabling co-production of natural pigments and industrial enzymes"],"prefix":"10.1016","volume":"97","author":[{"given":"Sarah","family":"Schmid","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charis","family":"Wardell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Maria","family":"Hatzl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara","family":"Steidl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Geier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Ebner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"Ellmer-Schaumberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Glieder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita","family":"Emmerstorfer-Augustin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymben.2026.102487_bib1","doi-asserted-by":"crossref","DOI":"10.1186\/1475-2859-9-24","article-title":"Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst","volume":"9","author":"Abad","year":"2010","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.ymben.2026.102487_bib2","article-title":"Pichia pastoris protease\u2010deficient and auxotrophic strains generated by a novel, user\u2010friendly vector toolbox for gene deletion","volume":"3426","author":"Ahmad","year":"2019","journal-title":"Yeast yea"},{"key":"10.1016\/j.ymben.2026.102487_bib3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13068-023-02374-4","article-title":"Combinatorial engineering of betalain biosynthesis pathway in yeast Saccharomyces cerevisiae","volume":"16","author":"Babaei","year":"2023","journal-title":"Biotechnol. Biofuels Bioprod."},{"key":"10.1016\/j.ymben.2026.102487_bib4","doi-asserted-by":"crossref","DOI":"10.3390\/biom13030441","article-title":"Industrial Production of Proteins with Pichia pastoris\u2014Komagataella phaffii","volume":"13","author":"Barone","year":"2023","journal-title":"Biomolecules"},{"key":"10.1016\/j.ymben.2026.102487_bib5","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2020.607028","article-title":"Komagataella phaffii as emerging model organism in fundamental research","volume":"11","author":"Bernauer","year":"2021","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ymben.2026.102487_bib6","doi-asserted-by":"crossref","first-page":"2515","DOI":"10.1021\/acssynbio.0c00299","article-title":"Identifying improved sites for heterologous gene integration using ATAC-seq","volume":"9","author":"Brady","year":"2020","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.ymben.2026.102487_bib7","doi-asserted-by":"crossref","DOI":"10.1186\/s12934-016-0540-5","article-title":"Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications","volume":"15","author":"Camattari","year":"2016","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.ymben.2026.102487_bib8","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.ymben.2014.07.008","article-title":"Metabolic engineering of Saccharomyces cerevisiae for the production of triacetic acid lactone","volume":"25","author":"Cardenas","year":"2014","journal-title":"Metab. Eng."},{"issue":"5 12","key":"10.1016\/j.ymben.2026.102487_bib9","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1038\/nmeth.3339","article-title":"Quantifying cellular capacity identifies gene expression designs with reduced burden","volume":"12","author":"Ceroni","year":"2015","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ymben.2026.102487_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12934-025-02649-5","article-title":"Development of a Komagataella phaffii cell factory for sustainable production of (+)-valencene","volume":"24","author":"Cheng","year":"2025","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.ymben.2026.102487_bib11","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/j.1574-6968.1997.tb10468.x","article-title":"Physiological and morphological effects of genetic alterations leading to a reduced synthesis of UDP-glucose in Saccharomyces cerevisiae","volume":"153","author":"Daran","year":"1997","journal-title":"FEMS Microbiol. Lett."},{"issue":"7 11","key":"10.1016\/j.ymben.2026.102487_bib12","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1038\/nchembio.1816","article-title":"An enzyme-coupled biosensor enables (S)-reticuline production in yeast from glucose","volume":"11","author":"Deloache","year":"2015","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.ymben.2026.102487_bib13","doi-asserted-by":"crossref","first-page":"7671","DOI":"10.1007\/s00253-014-5948-4","article-title":"Overexpression of membrane proteins from higher eukaryotes in yeasts","volume":"98","author":"Emmerstorfer","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymben.2026.102487_bib14","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/s004250050536","article-title":"Oxidative stress occurs during soybean nodule senescence","volume":"208","author":"Evans","year":"1999","journal-title":"Planta"},{"key":"10.1016\/j.ymben.2026.102487_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.yrtph.2021.105030","article-title":"Safety evaluation of food enzymes produced by a safe strain lineage of Bacillus subtilis","volume":"126","author":"Galano","year":"2021","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"10.1016\/j.ymben.2026.102487_bib17","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1021\/acssynbio.1c00307","article-title":"Synthetic biology toolkit for marker-less integration of multigene pathways into Pichia pastoris via CRISPR\/Cas9","volume":"11","author":"Gao","year":"2022","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.ymben.2026.102487_bib18","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1021\/acssynbio.1c00366","article-title":"Enhancing homologous recombination efficiency in Pichia pastoris for multiplex genome integration using short homology arms","volume":"11","author":"Gao","year":"2022","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.ymben.2026.102487_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2025.108593","article-title":"Technological advancement spurs Komagataella phaffii as a next-generation platform for sustainable biomanufacturing","volume":"82","author":"Gao","year":"2025","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.ymben.2026.102487_bib20","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1039\/C4CC08502G","article-title":"Compact multi-enzyme pathways in P. pastoris","volume":"51","author":"Geier","year":"2015","journal-title":"Chem. Commun."},{"key":"10.1016\/j.ymben.2026.102487_bib21","doi-asserted-by":"crossref","DOI":"10.1038\/nmeth.1318","article-title":"Enzymatic assembly of DNA molecules up to several hundred kilobases","volume":"6","author":"Gibson","year":"2009","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ymben.2026.102487_bib22","doi-asserted-by":"crossref","first-page":"2356","DOI":"10.1007\/s12010-024-05100-4","article-title":"Screening of plant UDP-Glycosyltransferases for betanin production in yeast","volume":"197","author":"Glitz","year":"2025","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.ymben.2026.102487_bib23","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.ymben.2017.12.008","article-title":"Bioproduction of a betalain color palette in Saccharomyces cerevisiae","volume":"45","author":"Grewal","year":"2018","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2026.102487_bib24","doi-asserted-by":"crossref","first-page":"3961","DOI":"10.1073\/pnas.72.10.3961","article-title":"Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene","volume":"72","author":"Grunstein","year":"1975","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"key":"10.1016\/j.ymben.2026.102487_bib25","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1186\/s40643-021-00460-9","article-title":"Metabolic engineering strategies for de novo biosynthesis of sterols and steroids in yeast","volume":"8","author":"Gu","year":"2021","journal-title":"Bioresour. Bioprocess."},{"key":"10.1016\/j.ymben.2026.102487_bib26","doi-asserted-by":"crossref","first-page":"e23","DOI":"10.1093\/nar\/30.6.e23","article-title":"A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast","volume":"30","author":"Gueldener","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ymben.2026.102487_bib27","doi-asserted-by":"crossref","first-page":"R41","DOI":"10.1111\/j.1750-3841.2006.00022.x","article-title":"Betalain stability and degradation - structural and chromatic aspects","volume":"71","author":"Herbach","year":"2006","journal-title":"J. Food Sci."},{"issue":"2 199","key":"10.1016\/j.ymben.2026.102487_bib28","first-page":"244","article-title":"Partial purification and characterization of UDP-glucose: betanidin 5-O- and 6-O-glucosyltransferases from cell suspension cultures of Dorotheanthus bellidiformis (Burm. f.) N.E.Br","volume":"199","author":"Heuer","year":"1996","journal-title":"Planta"},{"key":"10.1016\/j.ymben.2026.102487_bib29","doi-asserted-by":"crossref","DOI":"10.1093\/femsyr\/foy074","article-title":"Deletion of DNA ligase IV homolog confers higher gene targeting efficiency on homologous recombination in Komagataella phaffii","volume":"18","author":"Ito","year":"2018","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.ymben.2026.102487_bib30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13068-018-1227-4","article-title":"Astaxanthin overproduction in yeast by strain engineering and new gene target uncovering","volume":"11","author":"Jin","year":"2018","journal-title":"Biotechnol. Biofuels"},{"key":"10.1016\/j.ymben.2026.102487_bib31","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/j.jbiotec.2006.12.024","article-title":"Mixed feeds of glycerol and methanol can improve the performance of Pichia pastoris cultures: a quantitative study based on concentration gradients in transient continuous cultures","volume":"128","author":"Jungo","year":"2007","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.ymben.2026.102487_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2025.108576","article-title":"Biotechnological production and emerging applications of betalains: a review","volume":"81","author":"Khan","year":"2025","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.ymben.2026.102487_bib33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1475-2859-11-155","article-title":"De novo production of the flavonoid naringenin in engineered Saccharomyces cerevisiae","volume":"11","author":"Koopman","year":"2012","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.ymben.2026.102487_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2022.104888","article-title":"Applying the auxin-based degron system for the inducible, reversible and complete protein degradation in Komagataella phaffii","volume":"25","author":"Lehmayer","year":"2022","journal-title":"iScience"},{"key":"10.1016\/j.ymben.2026.102487_bib35","doi-asserted-by":"crossref","DOI":"10.2144\/05381BM04","article-title":"Condensed protocol for competent cell preparation and transformation of the methylotrophic yeast Pichia pastoris","volume":"38","author":"Lin-Cereghino","year":"2005","journal-title":"Biotechniques"},{"key":"10.1016\/j.ymben.2026.102487_bib36","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1186\/s12934-019-1194-x","article-title":"CRISPR-Cas9-mediated genomic multiloci integration in Pichia pastoris","volume":"18","author":"Liu","year":"2019","journal-title":"Microb. Cell Fact."},{"issue":"7622 537","key":"10.1016\/j.ymben.2026.102487_bib37","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1038\/nature19769","article-title":"Rewriting yeast central carbon metabolism for industrial isoprenoid production","volume":"537","author":"Meadows","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.ymben.2026.102487_bib38","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1007\/s12275-022-1580-7","article-title":"Assessment of Cre-lox and CRISPR-Cas9 as tools for recycling of multiple-integrated selection markers in Saccharomyces cerevisiae","volume":"60","author":"Moon","year":"2022","journal-title":"J. Microbiol."},{"key":"10.1016\/j.ymben.2026.102487_bib39","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1016\/j.cell.2016.02.004","article-title":"Engineering cellular metabolism","volume":"164","author":"Nielsen","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.ymben.2026.102487_bib40","doi-asserted-by":"crossref","DOI":"10.3390\/life10090186","article-title":"Rapid and efficient Colony-PCR for high throughput screening of genetically transformed Chlamydomonas reinhardtii","volume":"10","author":"Nouemssi","year":"2020","journal-title":"Life"},{"key":"10.1016\/j.ymben.2026.102487_bib41","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0039720","article-title":"Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology","volume":"7","author":"N\u00e4\u00e4tsaari","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.ymben.2026.102487_bib42","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.molp.2017.10.008","article-title":"\u201cLa Vie en Rose\u201d: biosynthesis, Sources, and Applications of Betalain Pigments","volume":"11","author":"Polturak","year":"2018","journal-title":"Mol. Plant"},{"key":"10.1016\/j.ymben.2026.102487_bib43","doi-asserted-by":"crossref","first-page":"2263","DOI":"10.1016\/j.celrep.2016.02.018","article-title":"Improved genome editing efficiency and flexibility using modified oligonucleotides with TALEN and CRISPR-Cas9 nucleases","volume":"14","author":"Renaud","year":"2016","journal-title":"Cell Rep."},{"key":"10.1016\/j.ymben.2026.102487_bib44","article-title":"Guidance on the characterisation of microorganisms used as feed additives or as production organisms","volume":"16","author":"Rychen","year":"2018","journal-title":"EFSA J."},{"key":"10.1016\/j.ymben.2026.102487_bib45","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0079-6603(08)60876-4","article-title":"The Flp Recombinase of th 2-\u03bcm Plasmid of Saccharomyces cerevisiae","volume":"51","author":"Sadowski","year":"1995","journal-title":"Prog. Nucleic Acid Res. Mol. Biol."},{"key":"10.1016\/j.ymben.2026.102487_bib46","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.febslet.2004.04.097","article-title":"Detection of UDP-glucose:cyclo-DOPA 5-O-glucosyltransferase activity in four o\u2019clocks (Mirabilis jalapa L.)","volume":"568","author":"Sasaki","year":"2004","journal-title":"FEBS Lett."},{"key":"10.1016\/j.ymben.2026.102487_bib47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12934-016-0486-7","article-title":"Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization","volume":"15","author":"Schwarzhans","year":"2016","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.ymben.2026.102487_bib48","article-title":"Scientific Opinion on the re-evaluation of beetroot red (E 162) as a food additive","volume":"13","year":"2015","journal-title":"EFSA J."},{"key":"10.1016\/j.ymben.2026.102487_bib49","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1021\/jf048751y","article-title":"Color, betalain pattern, and antioxidant properties of cactus pear (Opuntia spp.) clones","volume":"53","author":"Stintzing","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.ymben.2026.102487_bib50","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.procbio.2016.02.004","article-title":"Enhanced production of \u03b2-carotene in recombinant Saccharomyces cerevisiae by inverse metabolic engineering with supplementation of unsaturated fatty acids","volume":"51","author":"Sun","year":"2016","journal-title":"Process Biochem."},{"key":"10.1016\/j.ymben.2026.102487_bib51","doi-asserted-by":"crossref","first-page":"2290","DOI":"10.1038\/s41564-023-01517-5","article-title":"Beet red food colourant can be produced more sustainably with engineered Yarrowia lipolytica","volume":"8","author":"Thomsen","year":"2023","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.ymben.2026.102487_bib52","article-title":"Engineered bidirectional promoters enable rapid multi-gene co-expression optimization","volume":"9","author":"Vogl","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ymben.2026.102487_bib53","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.femsyr.2004.06.016","article-title":"Reliable high-throughput screening with Pichia pastoris by limiting yeast cell death phenomena","volume":"5","author":"Weis","year":"2004","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.ymben.2026.102487_bib54","doi-asserted-by":"crossref","DOI":"10.1186\/s12934-024-02368-3","article-title":"Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection","volume":"23","author":"Weiss","year":"2024","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.ymben.2026.102487_bib55","doi-asserted-by":"crossref","first-page":"3183","DOI":"10.1002\/jcb.26474","article-title":"Expanding the CRISPR\/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers","volume":"119","author":"Weninger","year":"2018","journal-title":"J. Cell. Biochem."},{"key":"10.1016\/j.ymben.2026.102487_bib56","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbiotec.2016.03.027","article-title":"Combinatorial optimization of CRISPR\/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris","author":"Weninger","year":"2016","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.ymben.2026.102487_bib57","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.ymben.2014.04.001","article-title":"Production of the sesquiterpenoid (+)-nootkatone by metabolic engineering of Pichia pastoris","volume":"24","author":"Wriessnegger","year":"2014","journal-title":"Metab. Eng."},{"key":"10.1016\/j.ymben.2026.102487_bib58","doi-asserted-by":"crossref","first-page":"12163","DOI":"10.1021\/jf2024769","article-title":"Antioxidant activity of betanidin: electrochemical study in aqueous media","volume":"59","author":"Wybraniec","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.ymben.2026.102487_bib59","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.synbio.2022.11.002","article-title":"Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering","volume":"8","author":"Zhang","year":"2023","journal-title":"Synth. Syst. Biotechnol."}],"container-title":["Metabolic Engineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096717626000820?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096717626000820?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:02:25Z","timestamp":1785412945000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1096717626000820"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":58,"alternative-id":["S1096717626000820"],"URL":"https:\/\/doi.org\/10.1016\/j.ymben.2026.102487","relation":{},"ISSN":["1096-7176"],"issn-type":[{"value":"1096-7176","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"MAPS: a marker-free genome integration system for Komagataella phaffii enabling co-production of natural pigments and industrial enzymes","name":"articletitle","label":"Article Title"},{"value":"Metabolic Engineering","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymben.2026.102487","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Published by Elsevier Inc. on behalf of International Metabolic Engineering Society.","name":"copyright","label":"Copyright"}],"article-number":"102487"}}