{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T00:44:48Z","timestamp":1773535488188,"version":"3.50.1"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH RO1","doi-asserted-by":"publisher","award":["HL088640"],"award-info":[{"award-number":["HL088640"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH RO1","doi-asserted-by":"publisher","award":["HL107418"],"award-info":[{"award-number":["HL107418"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Methods"],"published-print":{"date-parts":[[2015,10]]},"DOI":"10.1016\/j.ymeth.2015.06.019","type":"journal-article","created":{"date-parts":[[2015,6,30]],"date-time":"2015-06-30T02:13:35Z","timestamp":1435630415000},"page":"48-56","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["Resonant-scanning dual-color STED microscopy with ultrafast photon counting: A concise guide"],"prefix":"10.1016","volume":"88","author":[{"given":"Yong","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xundong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ligia","family":"Toro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrico","family":"Stefani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymeth.2015.06.019_b0005","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1364\/OL.19.000780","article-title":"Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy","volume":"19","author":"Hell","year":"1994","journal-title":"Opt. Lett."},{"key":"10.1016\/j.ymeth.2015.06.019_b0010","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1038\/nbt895","article-title":"Toward fluorescence nanoscopy","volume":"21","author":"Hell","year":"2003","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.ymeth.2015.06.019_b0015","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1046\/j.1365-2818.2000.00710.x","article-title":"Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy","volume":"198","author":"Gustafsson","year":"2000","journal-title":"J. Microsc."},{"key":"10.1016\/j.ymeth.2015.06.019_b0020","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.1126\/science.1127344","article-title":"Imaging intracellular fluorescent proteins at nanometer resolution","volume":"313","author":"Betzig","year":"2006","journal-title":"Science"},{"key":"10.1016\/j.ymeth.2015.06.019_b0025","doi-asserted-by":"crossref","first-page":"1302","DOI":"10.1364\/OE.18.001302","article-title":"Fast STED microscopy with continuous wave fiber lasers","volume":"18","author":"Moneron","year":"2010","journal-title":"Opt. Express"},{"key":"10.1016\/j.ymeth.2015.06.019_b0030","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1038\/nmeth1108","article-title":"STED microscopy with continuous wave beams","volume":"4","author":"Willig","year":"2007","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ymeth.2015.06.019_b0035","doi-asserted-by":"crossref","DOI":"10.1038\/nmeth.1624","article-title":"Sharper low-power STED nanoscopy by time gating","volume":"8","author":"Vicidomini","year":"2011","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ymeth.2015.06.019_b0040","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.ymeth.2013.06.029","article-title":"Gated CW-STED microscopy: a versatile tool for biological nanometer scale investigation","volume":"66","author":"Vicidomini","year":"2014","journal-title":"Methods"},{"key":"10.1016\/j.ymeth.2015.06.019_b0045","doi-asserted-by":"crossref","first-page":"4242","DOI":"10.1364\/OE.19.004242","article-title":"Time-gating improves the spatial resolution of STED microscopy","volume":"19","author":"Moffitt","year":"2011","journal-title":"Opt. Express"},{"key":"10.1016\/j.ymeth.2015.06.019_b0050","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1364\/OE.21.000210","article-title":"Frequency dependent detection in a STED microscope using modulated excitation light","volume":"21","author":"Ronzitti","year":"2013","journal-title":"Opt. Express"},{"key":"10.1016\/j.ymeth.2015.06.019_b0055","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1002\/jbio.201300208","article-title":"A new filtering technique for removing anti-Stokes emission background in gated CW-STED microscopy","volume":"7","author":"Hernandez","year":"2014","journal-title":"J. Biophotonics"},{"key":"10.1016\/j.ymeth.2015.06.019_b0060","first-page":"791","article-title":"Building a fast scanning stimulated emission depletion microscope: a step by step guide","volume":"vol. 2","author":"Gardeazabal Rodriguez","year":"2012"},{"key":"10.1016\/j.ymeth.2015.06.019_b0065","doi-asserted-by":"crossref","first-page":"11440","DOI":"10.1073\/pnas.0604965103","article-title":"Macromolecular-scale resolution in biological fluorescence microscopy","volume":"103","author":"Donnert","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ymeth.2015.06.019_b0070","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1038\/nmeth986","article-title":"Major signal increase in fluorescence microscopy through dark-state relaxation","volume":"4","author":"Donnert","year":"2007","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ymeth.2015.06.019_b0075","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1039\/b903357m","article-title":"Triplet-relaxation microscopy with bunched pulsed excitation","volume":"8","author":"Donnert","year":"2009","journal-title":"Photochem. Photobiol. Sci."},{"key":"10.1016\/j.ymeth.2015.06.019_b0080","doi-asserted-by":"crossref","unstructured":"Y. Wu, Resonant scanning with large field of view reduces photobleaching and enhances fluorescence yield in STED microscopy, arXiv: 1408.1507, 2015 (Ref. type: electronic citation).","DOI":"10.1038\/srep14766"},{"key":"10.1016\/j.ymeth.2015.06.019_b0085","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1126\/science.1154228","article-title":"Video-rate far-field optical nanoscopy dissects synaptic vesicle movement","volume":"320","author":"Westphal","year":"2008","journal-title":"Science"},{"key":"10.1016\/j.ymeth.2015.06.019_b0090","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/j.bpj.2014.12.007","article-title":"Self-calibrated line-scan STED-FCS to quantify lipid dynamics in model and cell membranes","volume":"108","author":"Benda","year":"2015","journal-title":"Biophys. J ."},{"key":"10.1016\/j.ymeth.2015.06.019_b0095","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms3093","article-title":"Stimulated emission depletion-based raster image correlation spectroscopy reveals biomolecular dynamics in live cells","volume":"4","author":"Hedde","year":"2013","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ymeth.2015.06.019_b0100","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms6412","article-title":"Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells","volume":"5","author":"Honigmann","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ymeth.2015.06.019_b0105","article-title":"Ultrafast photon counting applied to resonant scanning STED microscopy","author":"Wu","year":"2014","journal-title":"J. Microsc."},{"key":"10.1016\/j.ymeth.2015.06.019_b0110","doi-asserted-by":"crossref","first-page":"5123","DOI":"10.1364\/AO.42.005123","article-title":"Photostability of a fluorescent marker under pulsed excited-state depletion through stimulated emission","volume":"42","author":"Dyba","year":"2003","journal-title":"Appl. Opt."},{"key":"10.1016\/j.ymeth.2015.06.019_b0115","doi-asserted-by":"crossref","first-page":"1860","DOI":"10.1364\/OL.38.001860","article-title":"Auto-aligning stimulated emission depletion microscope using adaptive optics","volume":"38","author":"Gould","year":"2013","journal-title":"Opt. Lett."},{"key":"10.1016\/j.ymeth.2015.06.019_b0120","doi-asserted-by":"crossref","first-page":"20998","DOI":"10.1364\/OE.20.020998","article-title":"Adaptive optics enables 3D STED microscopy in aberrating specimens","volume":"20","author":"Gould","year":"2012","journal-title":"Opt. Express"},{"key":"10.1016\/j.ymeth.2015.06.019_b0125","doi-asserted-by":"crossref","DOI":"10.1088\/2040-8978\/12\/11\/115707","article-title":"Effects of polarization on the de-excitation dark focal spot in STED microscopy","volume":"12","author":"Hao","year":"2010","journal-title":"J. Opt."},{"key":"10.1016\/j.ymeth.2015.06.019_b0130","doi-asserted-by":"crossref","first-page":"2527","DOI":"10.1364\/OL.30.002527","article-title":"Focusing astigmatic Gaussian beams through optical systems with a high numerical aperture","volume":"30","author":"Gregor","year":"2005","journal-title":"Opt. Lett."},{"key":"10.1016\/j.ymeth.2015.06.019_b0135","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1054\/ceca.1999.0085","article-title":"Construction of a confocal microscope for real-time x\u2013y and x\u2013z imaging","volume":"26","author":"Callamaras","year":"1999","journal-title":"Cell Calcium"},{"key":"10.1016\/j.ymeth.2015.06.019_b0140","doi-asserted-by":"crossref","first-page":"16100","DOI":"10.1364\/OE.17.016100","article-title":"A STED microscope aligned by design","volume":"17","author":"Wildanger","year":"2009","journal-title":"Opt. Express"},{"key":"10.1016\/j.ymeth.2015.06.019_b0145","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1002\/jemt.22420","article-title":"A 3D aligning method for stimulated emission depletion microscopy using fluorescence lifetime distribution","volume":"77","author":"Wang","year":"2014","journal-title":"Microsc. Res. Tech."},{"key":"10.1016\/j.ymeth.2015.06.019_b0150","doi-asserted-by":"crossref","DOI":"10.1038\/srep02523","article-title":"Towards real-time image deconvolution: application to confocal and STED microscopy","volume":"3","author":"Zanella","year":"2013","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ymeth.2015.06.019_b0155","doi-asserted-by":"crossref","DOI":"10.1063\/1.4904092","article-title":"Multi-images deconvolution improves signal-to-noise ratio on gated stimulated emission depletion microscopy","volume":"105","author":"Castello","year":"2014","journal-title":"Appl. Phys. Lett."},{"key":"10.1016\/j.ymeth.2015.06.019_b0160","series-title":"Astronomical Image and Data Analysis","author":"Starck","year":"2006"},{"key":"10.1016\/j.ymeth.2015.06.019_b0165","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.bpj.2009.10.037","article-title":"Quantitative determination of spatial protein\u2013protein correlations in fluorescence confocal microscopy","volume":"98","author":"Wu","year":"2010","journal-title":"Biophys. J."},{"key":"10.1016\/j.ymeth.2015.06.019_b0170","doi-asserted-by":"crossref","first-page":"1554","DOI":"10.1038\/nprot.2011.384","article-title":"Quantifying spatial correlations of fluorescent markers using enhanced background reduction with protein proximity index and correlation coefficient estimations","volume":"6","author":"Zinchuk","year":"2011","journal-title":"Nat. Protoc."}],"container-title":["Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202315300062?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202315300062?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T01:31:01Z","timestamp":1760923861000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046202315300062"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10]]},"references-count":34,"alternative-id":["S1046202315300062"],"URL":"https:\/\/doi.org\/10.1016\/j.ymeth.2015.06.019","relation":{},"ISSN":["1046-2023"],"issn-type":[{"value":"1046-2023","type":"print"}],"subject":[],"published":{"date-parts":[[2015,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Resonant-scanning dual-color STED microscopy with ultrafast photon counting: A concise guide","name":"articletitle","label":"Article Title"},{"value":"Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymeth.2015.06.019","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}