{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T20:02:12Z","timestamp":1762459332205},"reference-count":51,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Methods"],"published-print":{"date-parts":[[2016,4]]},"DOI":"10.1016\/j.ymeth.2015.08.014","type":"journal-article","created":{"date-parts":[[2015,8,24]],"date-time":"2015-08-24T23:42:29Z","timestamp":1440459749000},"page":"128-134","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Bioengineering functional human sphincteric and non-sphincteric gastrointestinal smooth muscle constructs"],"prefix":"10.1016","volume":"99","author":[{"given":"Stephen L.","family":"Rego","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elie","family":"Zakhem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Orlando","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khalil N.","family":"Bitar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.ymeth.2015.08.014_b0005","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1016\/j.copbio.2013.03.021","article-title":"Tissue engineering and regenerative medicine as applied to the gastrointestinal tract","volume":"24","author":"Bitar","year":"2013","journal-title":"Curr. Opin. Biotechnol."},{"issue":"1 Suppl.","key":"10.1016\/j.ymeth.2015.08.014_b0010","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1097\/00006454-198601001-00005","article-title":"Anatomy and physiology of the gastrointestinal tract","volume":"5","author":"Van de Graaff","year":"1986","journal-title":"Pediatr. Infect. Dis."},{"issue":"4","key":"10.1016\/j.ymeth.2015.08.014_b0015","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1152\/physrev.00054.2006","article-title":"Hedgehog signaling in development and homeostasis of the gastrointestinal tract","volume":"87","author":"van den Brink","year":"2007","journal-title":"Physiol. Rev."},{"issue":"5 Pt 1","key":"10.1016\/j.ymeth.2015.08.014_b0020","first-page":"G869","article-title":"Lineages, and tissue interactions in the development of the enteric nervous system","volume":"275","author":"Gershon","year":"1998","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.ymeth.2015.08.014_b0025","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1002\/9780470942390.mo130179","article-title":"Establishment of gastrointestinal epithelial organoids","volume":"3","author":"Mahe","year":"2013","journal-title":"Curr. Protoc. Mouse Biol."},{"key":"10.1016\/j.ymeth.2015.08.014_b0030","doi-asserted-by":"crossref","DOI":"10.1038\/nature13863","article-title":"Modelling human development and disease in pluripotent stem-cell-derived gastric organoids","author":"McCracken","year":"2014","journal-title":"Nature"},{"issue":"5","key":"10.1016\/j.ymeth.2015.08.014_b0035","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.1053\/j.gastro.2011.07.050","article-title":"Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett\u2019s epithelium","volume":"141","author":"Sato","year":"2011","journal-title":"Gastroenterology"},{"issue":"6137","key":"10.1016\/j.ymeth.2015.08.014_b0040","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1126\/science.1234852","article-title":"Growing self-organizing mini-guts from a single intestinal stem cell: mechanism and applications","volume":"340","author":"Sato","year":"2013","journal-title":"Science"},{"issue":"18","key":"10.1016\/j.ymeth.2015.08.014_b0045","doi-asserted-by":"crossref","first-page":"3475","DOI":"10.1111\/j.1742-4658.2012.08646.x","article-title":"On the biomechanics of stem cell niche formation in the gut \u2013 modelling growing organoids","volume":"279","author":"Buske","year":"2012","journal-title":"FEBS J."},{"issue":"3","key":"10.1016\/j.ymeth.2015.08.014_b0050","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1074\/mcp.M112.021303","article-title":"Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids","volume":"12","author":"Tauro","year":"2013","journal-title":"Mol. Cell. Proteomics"},{"issue":"3","key":"10.1016\/j.ymeth.2015.08.014_b0055","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1038\/icb.1995.41","article-title":"Effect of TGF-beta on differentiated organoids of the colon carcinoma cell line LIM 1863","volume":"73","author":"Hayward","year":"1995","journal-title":"Immunol. Cell Biol."},{"issue":"10","key":"10.1016\/j.ymeth.2015.08.014_b0060","first-page":"2683","article-title":"A new colon carcinoma cell line (LIM1863) that grows as organoids with spontaneous differentiation into crypt-like structures in vitro","volume":"47","author":"Whitehead","year":"1987","journal-title":"Cancer Res."},{"issue":"5","key":"10.1016\/j.ymeth.2015.08.014_b0065","doi-asserted-by":"crossref","first-page":"G958","DOI":"10.1152\/ajpgi.00201.2009","article-title":"Role of thin-filament regulatory proteins in relaxation of colonic smooth muscle contraction","volume":"297","author":"Somara","year":"2009","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"issue":"5","key":"10.1016\/j.ymeth.2015.08.014_b0070","doi-asserted-by":"crossref","first-page":"G1164","DOI":"10.1152\/ajpgi.00479.2009","article-title":"Phosphorylated HSP20 modulates the association of thin-filament binding proteins: caldesmon with tropomyosin in colonic smooth muscle","volume":"299","author":"Somara","year":"2010","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"issue":"6","key":"10.1016\/j.ymeth.2015.08.014_b0075","doi-asserted-by":"crossref","first-page":"G1246","DOI":"10.1152\/ajpgi.90461.2008","article-title":"Direct association of calponin with specific domains of PKC-alpha","volume":"295","author":"Somara","year":"2008","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"issue":"3","key":"10.1016\/j.ymeth.2015.08.014_b0080","doi-asserted-by":"crossref","first-page":"361","DOI":"10.5858\/2001-125-0361-AOEBVI","article-title":"Absence of Epstein\u2013Barr virus in smooth muscle cells of idiopathic hypertrophic pyloric stenosis","volume":"125","author":"Jenson","year":"2001","journal-title":"Arch. Pathol. Lab. Med."},{"issue":"6","key":"10.1016\/j.ymeth.2015.08.014_b0085","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1203\/00006450-199906000-00012","article-title":"Smooth muscle cell hypertrophy versus hyperplasia in infantile hypertrophic pyloric stenosis","volume":"45","author":"Oue","year":"1999","journal-title":"Pediatr. Res."},{"issue":"6","key":"10.1016\/j.ymeth.2015.08.014_b0090","doi-asserted-by":"crossref","first-page":"1960","DOI":"10.1053\/j.gastro.2007.09.010","article-title":"Inducible mouse model of chronic intestinal pseudo-obstruction by smooth muscle-specific inactivation of the SRF gene","volume":"133","author":"Mericskay","year":"2007","journal-title":"Gastroenterology"},{"issue":"11","key":"10.1016\/j.ymeth.2015.08.014_b0095","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1136\/gut.2004.043968","article-title":"Advances in our understanding of the pathology of chronic intestinal pseudo-obstruction","volume":"53","author":"De Giorgio","year":"2004","journal-title":"Gut"},{"issue":"2","key":"10.1016\/j.ymeth.2015.08.014_b0100","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1006\/jsre.2001.6199","article-title":"Small bowel tissue engineering using small intestinal submucosa as a scaffold","volume":"99","author":"Chen","year":"2001","journal-title":"J. Surg. Res."},{"issue":"3","key":"10.1016\/j.ymeth.2015.08.014_b0105","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1089\/ten.1996.2.209","article-title":"Glycosaminoglycan content of small intestinal submucosa: a bioscaffold for tissue replacement","volume":"2","author":"Hodde","year":"1996","journal-title":"Tissue Eng."},{"issue":"4","key":"10.1016\/j.ymeth.2015.08.014_b0110","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1097\/00000637-199510000-00008","article-title":"Small intestinal submucosa: utilization for repair of rodent abdominal wall defects","volume":"35","author":"Prevel","year":"1995","journal-title":"Ann. Plast. Surg."},{"issue":"1","key":"10.1016\/j.ymeth.2015.08.014_b0115","doi-asserted-by":"crossref","first-page":"e21","DOI":"10.1016\/j.jss.2011.08.001","article-title":"Small intestinal submucosa seeded with intestinal smooth muscle cells in a rodent jejunal interposition model","volume":"171","author":"Qin","year":"2011","journal-title":"J. Surg. Res."},{"issue":"2","key":"10.1016\/j.ymeth.2015.08.014_b0120","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1089\/ten.2006.12.403","article-title":"Tissue engineering of small intestinal tissue using collagen sponge scaffolds seeded with smooth muscle cells","volume":"12","author":"Nakase","year":"2006","journal-title":"Tissue Eng."},{"issue":"3","key":"10.1016\/j.ymeth.2015.08.014_b0125","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1079\/PNS2003289","article-title":"Tissue engineering of the gastrointestinal tract for surgical replacement: a nutrition tool of the future?","volume":"62","author":"Grikscheit","year":"2003","journal-title":"Proc. Nutr. Soc."},{"issue":"9","key":"10.1016\/j.ymeth.2015.08.014_b0130","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1016\/S0022-3468(03)00386-5","article-title":"Tissue-engineered stomach: a preliminary report of a versatile in vivo model with therapeutic potential","volume":"38","author":"Grikscheit","year":"2003","journal-title":"J. Pediatr. Surg."},{"issue":"1","key":"10.1016\/j.ymeth.2015.08.014_b0135","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1097\/01.SLA.0000074964.77367.4a","article-title":"Tissue-engineered large intestine resembles native colon with appropriate in vitro physiology and architecture","volume":"238","author":"Grikscheit","year":"2003","journal-title":"Ann. Surg."},{"issue":"2","key":"10.1016\/j.ymeth.2015.08.014_b0140","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.jvs.2005.10.032","article-title":"Synthetic smooth muscle cell phenotype is associated with increased nicotinamide adenine dinucleotide phosphate oxidase activity: effect on collagen secretion","volume":"43","author":"Patel","year":"2006","journal-title":"J. Vasc. Surg."},{"issue":"2","key":"10.1016\/j.ymeth.2015.08.014_b0145","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s00441-004-0859-9","article-title":"Cultured gastrointestinal smooth muscle cells: cell response to contractile agonists depends on their phenotypic state","volume":"316","author":"Jarrousse","year":"2004","journal-title":"Cell Tissue Res."},{"issue":"Suppl. 3A","key":"10.1016\/j.ymeth.2015.08.014_b0150","doi-asserted-by":"crossref","first-page":"15S","DOI":"10.1016\/S0002-9343(03)00189-X","article-title":"Function of gastrointestinal smooth muscle: from signaling to contractile proteins","volume":"115","author":"Bitar","year":"2003","journal-title":"Am. J. Med."},{"issue":"6\u20137","key":"10.1016\/j.ymeth.2015.08.014_b0155","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/S0024-3205(98)00584-0","article-title":"Contractile role of M2 and M3 muscarinic receptors in gastrointestinal smooth muscle","volume":"64","author":"Ehlert","year":"1999","journal-title":"Life Sci."},{"issue":"6 Pt 1","key":"10.1016\/j.ymeth.2015.08.014_b0160","first-page":"G958","article-title":"Myosin phosphorylation and calcium in tonic and phasic contractions of colonic smooth muscle","volume":"260","author":"Gerthoffer","year":"1991","journal-title":"Am. J. Physiol."},{"issue":"1","key":"10.1016\/j.ymeth.2015.08.014_b0165","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1053\/j.gastro.2009.03.036","article-title":"Bioengineered internal anal sphincter derived from isolated human internal anal sphincter smooth muscle cells","volume":"137","author":"Somara","year":"2009","journal-title":"Gastroenterology"},{"issue":"5","key":"10.1016\/j.ymeth.2015.08.014_b0170","doi-asserted-by":"crossref","first-page":"G830","DOI":"10.1152\/ajpgi.00130.2006","article-title":"Spontaneously tonic smooth muscle has characteristically higher levels of RhoA\/ROK compared with the phasic smooth muscle","volume":"291","author":"Patel","year":"2006","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"issue":"9","key":"10.1016\/j.ymeth.2015.08.014_b0175","doi-asserted-by":"crossref","first-page":"e74608","DOI":"10.1371\/journal.pone.0074608","article-title":"Tonic and phasic smooth muscle contraction is not regulated by the PKCalpha \u2013 CPI-17 pathway in swine stomach antrum and fundus","volume":"8","author":"Zhang","year":"2013","journal-title":"PLoS ONE"},{"issue":"Pt 10","key":"10.1016\/j.ymeth.2015.08.014_b0180","doi-asserted-by":"crossref","first-page":"2207","DOI":"10.1242\/jcs.115.10.2207","article-title":"Agonist-induced phasic and tonic responses in smooth muscle are mediated by InsP(3)","volume":"115","author":"McCarron","year":"2002","journal-title":"J. Cell Sci."},{"issue":"4","key":"10.1016\/j.ymeth.2015.08.014_b0185","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1111\/j.1748-1716.1980.tb06612.x","article-title":"Effects of phasic and tonic activation on contraction dynamics in smooth muscle","volume":"109","author":"Uvelius","year":"1980","journal-title":"Acta Physiol. Scand."},{"issue":"3","key":"10.1016\/j.ymeth.2015.08.014_b0190","first-page":"227","article-title":"Phasic and tonic types of smooth muscle activity in lower oesophageal sphincter and stomach of the dog","volume":"26","author":"Golenhofen","year":"1979","journal-title":"Acta Hepatogastroenterol. (Stuttg)"},{"issue":"28","key":"10.1016\/j.ymeth.2015.08.014_b0195","doi-asserted-by":"crossref","first-page":"6649","DOI":"10.1016\/j.biomaterials.2013.05.023","article-title":"Neuroglial differentiation of adult enteric neuronal progenitor cells as a function of extracellular matrix composition","volume":"34","author":"Raghavan","year":"2013","journal-title":"Biomaterials"},{"key":"10.1016\/j.ymeth.2015.08.014_b0200","doi-asserted-by":"crossref","DOI":"10.1007\/s13365-014-0259-1","article-title":"Infected peripheral blood mononuclear cells transmit latent varicella zoster virus infection to the guinea pig enteric nervous system","author":"Gan","year":"2014","journal-title":"J. Neurovirol."},{"issue":"11\u201312","key":"10.1016\/j.ymeth.2015.08.014_b0205","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1089\/ten.tea.2013.0422","article-title":"Bioengineering of physiologically functional intrinsically innervated human internal anal sphincter constructs","volume":"20","author":"Gilmont","year":"2014","journal-title":"Tissue Eng. Part A"},{"issue":"6","key":"10.1016\/j.ymeth.2015.08.014_b0210","doi-asserted-by":"crossref","first-page":"1882","DOI":"10.1016\/j.biomaterials.2013.11.049","article-title":"Neo-innervation of a bioengineered intestinal smooth muscle construct around chitosan scaffold","volume":"35","author":"Zakhem","year":"2014","journal-title":"Biomaterials"},{"issue":"11","key":"10.1016\/j.ymeth.2015.08.014_b0215","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1089\/jir.2013.0009","article-title":"Soluble tumor necrosis factor receptors shed by breast tumor cells inhibit macrophage chemotaxis","volume":"33","author":"Rego","year":"2013","journal-title":"J. Interferon Cytokine Res."},{"issue":"3","key":"10.1016\/j.ymeth.2015.08.014_b0220","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1007\/s10456-013-9405-2","article-title":"Breast tumor cell TACE-shed MCSF promotes pro-angiogenic macrophages through NF-kappaB signaling","volume":"17","author":"Rego","year":"2014","journal-title":"Angiogenesis"},{"issue":"9","key":"10.1016\/j.ymeth.2015.08.014_b0225","doi-asserted-by":"crossref","first-page":"2634","DOI":"10.1172\/JCI38864","article-title":"Acquisition of the contractile phenotype by murine arterial smooth muscle cells depends on the Mir143\/145 gene cluster","volume":"119","author":"Boettger","year":"2009","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.ymeth.2015.08.014_b0230","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0079-6123(08)62099-3","article-title":"The role of G-protein coupled receptor kinases in the regulation of muscarinic cholinergic receptors","volume":"109","author":"Hosey","year":"1996","journal-title":"Prog. Brain Res."},{"issue":"11","key":"10.1016\/j.ymeth.2015.08.014_b0235","doi-asserted-by":"crossref","first-page":"3287","DOI":"10.1073\/pnas.69.11.3287","article-title":"Role of muscarinic cholinergic receptors in regulation of guanosine 3\u2032:5\u2032-cyclic monophosphate content in mammalian brain, heart muscle, and intestinal smooth muscle","volume":"69","author":"Lee","year":"1972","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"2","key":"10.1016\/j.ymeth.2015.08.014_b0240","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1002\/1097-0177(2000)9999:9999<::AID-DVDY1047>3.3.CO;2-Y","article-title":"Molecular mechanisms of development of the gastrointestinal tract","volume":"219","author":"Roberts","year":"2000","journal-title":"Dev. Dyn."},{"issue":"2","key":"10.1016\/j.ymeth.2015.08.014_b0245","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.cell.2014.04.050","article-title":"Crosstalk between muscularis macrophages and enteric neurons regulates gastrointestinal motility","volume":"158","author":"Muller","year":"2014","journal-title":"Cell"},{"key":"10.1016\/j.ymeth.2015.08.014_b0250","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/978-0-387-46954-6_11","article-title":"Neural crest and the development of the enteric nervous system","volume":"589","author":"Anderson","year":"2006","journal-title":"Adv. Exp. Med. Biol."},{"issue":"2","key":"10.1016\/j.ymeth.2015.08.014_b0255","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1002\/neu.480240207","article-title":"From neural crest to bowel: development of the enteric nervous system","volume":"24","author":"Gershon","year":"1993","journal-title":"J. Neurobiol."}],"container-title":["Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S104620231530058X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S104620231530058X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,5,20]],"date-time":"2022-05-20T21:51:49Z","timestamp":1653083509000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S104620231530058X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4]]},"references-count":51,"alternative-id":["S104620231530058X"],"URL":"https:\/\/doi.org\/10.1016\/j.ymeth.2015.08.014","relation":{},"ISSN":["1046-2023"],"issn-type":[{"value":"1046-2023","type":"print"}],"subject":[],"published":{"date-parts":[[2016,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Bioengineering functional human sphincteric and non-sphincteric gastrointestinal smooth muscle constructs","name":"articletitle","label":"Article Title"},{"value":"Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymeth.2015.08.014","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}