{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:40:10Z","timestamp":1792665610799,"version":"4.5.0"},"reference-count":126,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agence National pour la Recherche"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Methods"],"published-print":{"date-parts":[[2017,8]]},"DOI":"10.1016\/j.ymeth.2017.05.007","type":"journal-article","created":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T02:01:10Z","timestamp":1494900070000},"page":"12-22","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"special_numbering":"C","title":["Imaging macropinosomes during Shigella infections"],"prefix":"10.1016","volume":"127","author":[{"given":"Sonja","family":"K\u00fchn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noelia","family":"Lopez-Montero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuen-Yan","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Sartori-Rupp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jost","family":"Enninga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymeth.2017.05.007_b0005","doi-asserted-by":"crossref","first-page":"374","DOI":"10.3389\/fphys.2014.00374","article-title":"Small GTPases and phosphoinositides in the regulatory mechanisms of macropinosome formation and maturation","volume":"5","author":"Egami","year":"2014","journal-title":"Front. Physiol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0010","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1083\/jcb.124.5.689","article-title":"The coated pit and macropinocytic pathways serve distinct endosome populations","volume":"124","author":"Hewlett","year":"1994","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0015","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1038\/icb.2011.20","article-title":"Macropinocytosis: an endocytic pathway for internalising large gulps","volume":"89","author":"Lim","year":"2011","journal-title":"Immunol. Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0020","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1038\/nrm2447","article-title":"Shaping cups into phagosomes and macropinosomes","volume":"9","author":"Swanson","year":"2008","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0025","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/S0962-8924(00)89101-1","article-title":"Macropinocytosis","volume":"5","author":"Swanson","year":"1995","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0030","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1111\/j.1600-0854.2009.00878.x","article-title":"Defining macropinocytosis","volume":"10","author":"Kerr","year":"2009","journal-title":"Traffic"},{"key":"10.1016\/j.ymeth.2017.05.007_b0035","doi-asserted-by":"crossref","first-page":"4577","DOI":"10.1093\/emboj\/19.17.4577","article-title":"Localization of phosphatidylinositol 3-phosphate in yeast and mammalian cells","volume":"19","author":"Gillooly","year":"2000","journal-title":"EMBO J."},{"key":"10.1016\/j.ymeth.2017.05.007_b0040","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1083\/jcb.135.5.1249","article-title":"A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages","volume":"135","author":"Araki","year":"1996","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0045","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a016972","article-title":"Endocytosis of viruses and bacteria","volume":"6","author":"Cossart","year":"2014","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0050","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1038\/364639a0","article-title":"Ruffles induced by Salmonella and other stimuli direct macropinocytosis of bacteria","volume":"364","author":"Francis","year":"1993","journal-title":"Nature"},{"key":"10.1016\/j.ymeth.2017.05.007_b0055","doi-asserted-by":"crossref","first-page":"e1005602","DOI":"10.1371\/journal.ppat.1005602","article-title":"Macropinosomes are key players in early shigella invasion and vacuolar escape in epithelial cells","volume":"12","author":"Weiner","year":"2016","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.ymeth.2017.05.007_b0060","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1046\/j.1462-5822.2002.00195.x","article-title":"Modulation of Brucella-induced macropinocytosis by lipid rafts mediates intracellular replication","volume":"4","author":"Watarai","year":"2002","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0065","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/S0882-4010(03)00089-5","article-title":"Internalization of Mycobacterium tuberculosis by macropinocytosis in non-phagocytic cells","volume":"35","author":"Garcia-Perez","year":"2003","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.ymeth.2017.05.007_b0070","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1186\/1471-2180-12-246","article-title":"Macropinocytosis is responsible for the uptake of pathogenic and non-pathogenic mycobacteria by B lymphocytes (Raji cells)","volume":"12","author":"Garcia-Perez","year":"2012","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0075","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1111\/j.1462-5822.2011.01615.x","article-title":"Internalization, phagolysosomal biogenesis and killing of mycobacteria in enucleated epithelial cells","volume":"13","author":"de Souza Carvalho","year":"2011","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0080","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.semcdb.2016.07.019","article-title":"Manipulation of host membranes by the bacterial pathogens Listeria, Francisella, Shigella and Yersinia","volume":"60","author":"Pizarro-Cerda","year":"2016","journal-title":"Semin. Cell Dev. Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0085","doi-asserted-by":"crossref","first-page":"e353","DOI":"10.1371\/journal.pmed.0030353","article-title":"A multicentre study of Shigella diarrhoea in six Asian countries: disease burden, clinical manifestations, and microbiology","volume":"3","author":"von Seidlein","year":"2006","journal-title":"PLoS Med."},{"key":"10.1016\/j.ymeth.2017.05.007_b0090","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1083\/jcb.146.6.1319","article-title":"Activation of the CDC42 effector N-WASP by the Shigella flexneri IcsA protein promotes actin nucleation by Arp2\/3 complex and bacterial actin-based motility","volume":"146","author":"Egile","year":"1999","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0095","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1126\/science.1090124","article-title":"Bacterial invasion: the paradigms of enteroinvasive pathogens","volume":"304","author":"Cossart","year":"2004","journal-title":"Science"},{"key":"10.1016\/j.ymeth.2017.05.007_b0100","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1128\/CMR.00032-07","article-title":"Molecular pathogenesis of Shigella spp.: controlling host cell signaling, invasion, and death by type III secretion","volume":"21","author":"Schroeder","year":"2008","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.ymeth.2017.05.007_b0105","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1083\/jcb.147.3.683","article-title":"The tripartite type III secreton of Shigella flexneri inserts IpaB and IpaC into host membranes","volume":"147","author":"Blocker","year":"1999","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0110","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1038\/nrmicro1526","article-title":"The type III secretion injectisome","volume":"4","author":"Cornelis","year":"2006","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0115","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1038\/nature05272","article-title":"Protein delivery into eukaryotic cells by type III secretion machines","volume":"444","author":"Galan","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.ymeth.2017.05.007_b0120","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.mib.2008.12.002","article-title":"Shigella type III secretion effectors: how, where, when, for what purposes?","volume":"12","author":"Parsot","year":"2009","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0125","doi-asserted-by":"crossref","first-page":"3315","DOI":"10.1002\/j.1460-2075.1996.tb00696.x","article-title":"Rho-dependent membrane folding causes Shigella entry into epithelial cells","volume":"15","author":"Adam","year":"1996","journal-title":"EMBO J."},{"key":"10.1016\/j.ymeth.2017.05.007_b0130","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1023\/A:1001519806727","article-title":"Molecular bases of epithelial cell invasion by Shigella flexneri","volume":"74","author":"Sansonetti","year":"1998","journal-title":"Antonie Van Leeuwenhoek"},{"key":"10.1016\/j.ymeth.2017.05.007_b0135","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1111\/cmi.12400","article-title":"Cytoskeletal mechanics during Shigella invasion and dissemination in epithelial cells","volume":"17","author":"Valencia-Gallardo","year":"2015","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0140","doi-asserted-by":"crossref","first-page":"a016717","DOI":"10.1101\/cshperspect.a016717","article-title":"The inside story of Shigella invasion of intestinal epithelial cells","volume":"3","author":"Carayol","year":"2013","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"10.1016\/j.ymeth.2017.05.007_b0145","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1111\/j.1462-5822.2010.01428.x","article-title":"Tracking the dynamic interplay between bacterial and host factors during pathogen-induced vacuole rupture in real time","volume":"12","author":"Ray","year":"2010","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0150","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/nrmicro2112","article-title":"Life on the inside: the intracellular lifestyle of cytosolic bacteria","volume":"7","author":"Ray","year":"2009","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0155","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.chom.2014.09.005","article-title":"Shigella subverts the host recycling compartment to rupture its vacuole","volume":"16","author":"Mellouk","year":"2014","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.ymeth.2017.05.007_b0160","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/978-1-4939-3170-5_15","article-title":"Guidelines for the use of protein domains in acidic phospholipid imaging","volume":"1376","author":"Platre","year":"2016","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0165","doi-asserted-by":"crossref","first-page":"2887","DOI":"10.1039\/b901966a","article-title":"The fluorescent protein palette: tools for cellular imaging","volume":"38","author":"Day","year":"2009","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.ymeth.2017.05.007_b0170","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S1074-5521(03)00068-1","article-title":"Directed evolution of O6-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo","volume":"10","author":"Juillerat","year":"2003","journal-title":"Chem. Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0175","first-page":"195","article-title":"The HaloTag: a novel technology for cell imaging and protein analysis","volume":"356","author":"Los","year":"2007","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0180","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.chembiol.2008.01.007","article-title":"An engineered protein tag for multiprotein labeling in living cells","volume":"15","author":"Gautier","year":"2008","journal-title":"Chem. Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0185","doi-asserted-by":"crossref","first-page":"6063","DOI":"10.1021\/ja017687n","article-title":"New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications","volume":"124","author":"Adams","year":"2002","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.ymeth.2017.05.007_b0190","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1038\/nbt1136","article-title":"Mammalian cell-based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity","volume":"23","author":"Martin","year":"2005","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0195","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.tibtech.2011.08.002","article-title":"Imaging proteins inside cells with fluorescent tags","volume":"30","author":"Crivat","year":"2012","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0200","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/boc.201600040","article-title":"Recent developments of genetically encoded optical sensors for cell biology","volume":"109","author":"Bolbat","year":"2017","journal-title":"Biol. Cell"},{"key":"10.1016\/j.ymeth.2017.05.007_b0205","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1038\/nrm3379","article-title":"Coordination between RAB GTPase and phosphoinositide regulation and functions","volume":"13","author":"Jean","year":"2012","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0210","doi-asserted-by":"crossref","first-page":"E978","DOI":"10.1073\/pnas.1311029111","article-title":"Sequential breakdown of 3-phosphorylated phosphoinositides is essential for the completion of macropinocytosis","volume":"111","author":"Maekawa","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ymeth.2017.05.007_b0215","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1042\/BJ20090428","article-title":"Immunocytochemical techniques reveal multiple, distinct cellular pools of PtdIns4P and PtdIns(4,5)P(2)","volume":"422","author":"Hammond","year":"2009","journal-title":"Biochem. J."},{"key":"10.1016\/j.ymeth.2017.05.007_b0220","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1016\/j.bbalip.2006.03.019","article-title":"Live cell imaging of phosphoinositide dynamics with fluorescent protein domains","volume":"1761","author":"Varnai","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ymeth.2017.05.007_b0225","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1038\/35083076","article-title":"PtdIns(3)P regulates the neutrophil oxidase complex by binding to the PX domain of p40(phox)","volume":"3","author":"Ellson","year":"2001","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0230","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1038\/35083070","article-title":"The PX domains of p47phox and p40phox bind to lipid products of PI(3)K","volume":"3","author":"Kanai","year":"2001","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0235","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1038\/nchembio.390","article-title":"Translation of the phosphoinositide code by PI effectors","volume":"6","author":"Kutateladze","year":"2010","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0240","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1038\/nrm2328","article-title":"Membrane recognition by phospholipid-binding domains","volume":"9","author":"Lemmon","year":"2008","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0245","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00839-13","article-title":"Live-cell imaging of phosphoinositide dynamics and membrane architecture during Legionella infection","volume":"5","author":"Weber","year":"2014","journal-title":"MBio"},{"key":"10.1016\/j.ymeth.2017.05.007_b0250","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/0092-8674(90)90369-P","article-title":"Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments","volume":"62","author":"Chavrier","year":"1990","journal-title":"Cell"},{"key":"10.1016\/j.ymeth.2017.05.007_b0255","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1152\/physrev.00059.2009","article-title":"Role of Rab GTPases in membrane traffic and cell physiology","volume":"91","author":"Hutagalung","year":"2011","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.ymeth.2017.05.007_b0260","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/S0962-8924(01)02147-X","article-title":"Rab GTPases: specifying and deciphering organelle identity and function","volume":"11","author":"Pfeffer","year":"2001","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0265","doi-asserted-by":"crossref","first-page":"3250","DOI":"10.1242\/jcs.053207","article-title":"Sequential signaling in plasma-membrane domains during macropinosome formation in macrophages","volume":"122","author":"Yoshida","year":"2009","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.ymeth.2017.05.007_b0270","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1111\/tra.12290","article-title":"Vaccinia virus infection requires maturation of macropinosomes","volume":"16","author":"Rizopoulos","year":"2015","journal-title":"Traffic"},{"key":"10.1016\/j.ymeth.2017.05.007_b0275","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1111\/cmi.12415","article-title":"Infectious bursal disease virus uptake involves macropinocytosis and trafficking to early endosomes in a Rab5-dependent manner","volume":"17","author":"Gimenez","year":"2015","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0280","doi-asserted-by":"crossref","first-page":"e1002657","DOI":"10.1371\/journal.ppat.1002657","article-title":"Entry of human papillomavirus type 16 by actin-dependent, clathrin- and lipid raft-independent endocytosis","volume":"8","author":"Schelhaas","year":"2012","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.ymeth.2017.05.007_b0285","doi-asserted-by":"crossref","first-page":"e1140615","DOI":"10.1080\/21592799.2016.1140615","article-title":"Sequential recruitment of Rab GTPases during early stages of phagocytosis","volume":"6","author":"Yeo","year":"2016","journal-title":"Cell Logist."},{"key":"10.1016\/j.ymeth.2017.05.007_b0290","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1083\/jcb.201312072","article-title":"A novel probe for phosphatidylinositol 4-phosphate reveals multiple pools beyond the Golgi","volume":"205","author":"Hammond","year":"2014","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0295","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1038\/28879","article-title":"EEA1 links PI(3)K function to Rab5 regulation of endosome fusion","volume":"394","author":"Simonsen","year":"1998","journal-title":"Nature"},{"key":"10.1016\/j.ymeth.2017.05.007_b0300","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1038\/nrm2728","article-title":"Rab GTPases as coordinators of vesicle traffic","volume":"10","author":"Stenmark","year":"2009","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0305","doi-asserted-by":"crossref","first-page":"21327","DOI":"10.1016\/S0021-9258(18)54636-6","article-title":"Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficking","volume":"266","author":"Fukuda","year":"1991","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ymeth.2017.05.007_b0310","doi-asserted-by":"crossref","first-page":"3481","DOI":"10.1038\/emboj.2011.286","article-title":"Endosome maturation","volume":"30","author":"Huotari","year":"2011","journal-title":"EMBO J."},{"key":"10.1016\/j.ymeth.2017.05.007_b0315","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1152\/physrev.00002.2012","article-title":"Role of phospholipids in endocytosis, phagocytosis, and macropinocytosis","volume":"93","author":"Bohdanowicz","year":"2013","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.ymeth.2017.05.007_b0320","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1038\/mt.2013.54","article-title":"Endocytosis of gene delivery vectors: from clathrin-dependent to lipid raft-mediated endocytosis","volume":"21","author":"El-Sayed","year":"2013","journal-title":"Mol. Ther."},{"key":"10.1016\/j.ymeth.2017.05.007_b0325","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1002\/bies.201300132","article-title":"Phosphatidylinositol 5-phosphate: a nuclear stress lipid and a tuner of membranes and cytoskeleton dynamics","volume":"36","author":"Viaud","year":"2014","journal-title":"BioEssays"},{"key":"10.1016\/j.ymeth.2017.05.007_b0330","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/0092-8674(92)90306-W","article-title":"The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway","volume":"70","author":"Bucci","year":"1992","journal-title":"Cell"},{"key":"10.1016\/j.ymeth.2017.05.007_b0335","doi-asserted-by":"crossref","first-page":"9649","DOI":"10.1074\/jbc.M010387200","article-title":"Receptor and membrane recycling can occur with unaltered efficiency despite dramatic Rab5(q79l)-induced changes in endosome geometry","volume":"276","author":"Ceresa","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ymeth.2017.05.007_b0340","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1002\/j.1460-2075.1994.tb06381.x","article-title":"Inhibition of rab5 GTPase activity stimulates membrane fusion in endocytosis","volume":"13","author":"Stenmark","year":"1994","journal-title":"EMBO J."},{"key":"10.1016\/j.ymeth.2017.05.007_b0345","doi-asserted-by":"crossref","first-page":"10337","DOI":"10.1074\/jbc.272.16.10337","article-title":"Uncoupling of membrane ruffling and pinocytosis during Ras signal transduction","volume":"272","author":"Li","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ymeth.2017.05.007_b0350","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1111\/tpj.12358","article-title":"A multi-colour\/multi-affinity marker set to visualize phosphoinositide dynamics in Arabidopsis","volume":"77","author":"Simon","year":"2014","journal-title":"Plant J."},{"key":"10.1016\/j.ymeth.2017.05.007_b0355","doi-asserted-by":"crossref","first-page":"e10611","DOI":"10.1371\/journal.pone.0010611","article-title":"Systematic comparison of constitutive promoters and the doxycycline-inducible promoter","volume":"5","author":"Qin","year":"2010","journal-title":"PLoS ONE"},{"key":"10.1016\/j.ymeth.2017.05.007_b0360","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/nmeth867","article-title":"Analyzing phosphoinositides and their interacting proteins","volume":"3","author":"Rusten","year":"2006","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ymeth.2017.05.007_b0365","first-page":"736","article-title":"Detection and manipulation of phosphoinositides","volume":"2015","author":"Idevall-Hagren","year":"1851","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ymeth.2017.05.007_b0370","doi-asserted-by":"crossref","first-page":"e1174798","DOI":"10.1080\/19420889.2016.1174798","article-title":"Deciphering the roles of phosphoinositide lipids in phagolysosome biogenesis","volume":"9","author":"Jeschke","year":"2016","journal-title":"Commun. Integr. Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0375","first-page":"1031","article-title":"Multiple bonds for the lipid interest","volume":"2014","author":"Kuerschner","year":"1841","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ymeth.2017.05.007_b0380","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1038\/nmeth728","article-title":"Polyene-lipids: a new tool to image lipids","volume":"2","author":"Kuerschner","year":"2005","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ymeth.2017.05.007_b0385","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1002\/anie.200805507","article-title":"Selective fluorescence labeling of lipids in living cells","volume":"48","author":"Neef","year":"2009","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.ymeth.2017.05.007_b0390","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/nchembio0605-13","article-title":"Chemistry in living systems","volume":"1","author":"Prescher","year":"2005","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0395","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1038\/nchembio.348","article-title":"Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells","volume":"6","author":"Subramanian","year":"2010","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0400","doi-asserted-by":"crossref","first-page":"2022","DOI":"10.1161\/ATVBAHA.110.210849","article-title":"Native low-density lipoprotein uptake by macrophage colony-stimulating factor-differentiated human macrophages is mediated by macropinocytosis and micropinocytosis","volume":"30","author":"Anzinger","year":"2010","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0405","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1038\/nprot.2014.004","article-title":"Determining the macropinocytic index of cells through a quantitative image-based assay","volume":"9","author":"Commisso","year":"2014","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ymeth.2017.05.007_b0410","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2015.00001","article-title":"Macropinocytosis in phagocytes: regulation of MHC class-II-restricted antigen presentation in dendritic cells","volume":"6","author":"Liu","year":"2015","journal-title":"Front. Physiol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0415","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1186\/1471-2180-12-246","article-title":"Macropinocytosis is responsible for the uptake of pathogenic and non-pathogenic mycobacteria by B lymphocytes (Raji cells)","volume":"12","author":"Garc\u00eda-P\u00e9rez","year":"2012","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0420","doi-asserted-by":"crossref","first-page":"10780","DOI":"10.1074\/jbc.M112.362178","article-title":"Temporal transcriptional response during infection of type II alveolar epithelial cells with Francisella tularensis live vaccine strain (LVS) supports a general host suppression and bacterial uptake by macropinocytosis","volume":"288","author":"Bradburne","year":"2013","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ymeth.2017.05.007_b0425","doi-asserted-by":"crossref","first-page":"e1005602","DOI":"10.1371\/journal.ppat.1005602","article-title":"Macropinosomes are key players in early Shigella invasion and vacuolar escape in epithelial cells","volume":"12","author":"Weiner","year":"2016","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.ymeth.2017.05.007_b0430","doi-asserted-by":"crossref","first-page":"4641","DOI":"10.1128\/iai.62.10.4641-4645.1994","article-title":"Salmonella invasion of nonphagocytic cells induces formation of macropinosomes in the host cell","volume":"62","author":"Garcia-del Portillo","year":"1994","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ymeth.2017.05.007_b0435","doi-asserted-by":"crossref","first-page":"2857","DOI":"10.1091\/mbc.e07-10-1094","article-title":"A Raft-derived, Pak1-regulated entry participates in alpha2beta1 integrin-dependent sorting to caveosomes","volume":"19","author":"Karjalainen","year":"2008","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.ymeth.2017.05.007_b0440","doi-asserted-by":"crossref","first-page":"e1003941","DOI":"10.1371\/journal.ppat.1003941","article-title":"CIB1 synergizes with EphrinA2 to regulate Kaposi's sarcoma-associated herpesvirus macropinocytic entry in human microvascular dermal endothelial cells","volume":"10","author":"Bandyopadhyay","year":"2014","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.ymeth.2017.05.007_b0445","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1038\/emboj.2008.38","article-title":"Subversion of CtBP1-controlled macropinocytosis by human adenovirus serotype 3","volume":"27","author":"Amstutz","year":"2008","journal-title":"EMBO J."},{"key":"10.1016\/j.ymeth.2017.05.007_b0450","doi-asserted-by":"crossref","first-page":"5336","DOI":"10.1128\/JVI.02494-09","article-title":"Macropinocytotic uptake and infection of human epithelial cells with species B2 adenovirus type 35","volume":"84","author":"Kalin","year":"2010","journal-title":"J. Virol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0455","doi-asserted-by":"crossref","first-page":"10950","DOI":"10.1128\/JVI.05992-11","article-title":"Filamentous influenza virus enters cells via macropinocytosis","volume":"86","author":"Rossman","year":"2012","journal-title":"J. Virol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0460","doi-asserted-by":"crossref","first-page":"e1005595","DOI":"10.1371\/journal.ppat.1005595","article-title":"African swine fever virus undergoes outer envelope disruption, capsid disassembly and inner envelope fusion before core release from multivesicular endosomes","volume":"12","author":"Hernaez","year":"2016","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.ymeth.2017.05.007_b0465","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1002\/cbin.10424","article-title":"The effect of the size of fluorescent dextran on its endocytic pathway","volume":"39","author":"Li","year":"2015","journal-title":"Cell Biol. Int."},{"key":"10.1016\/j.ymeth.2017.05.007_b0470","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.mex.2014.05.002","article-title":"Macropinosome quantitation assay","volume":"1","author":"Wang","year":"2014","journal-title":"MethodsX"},{"key":"10.1016\/j.ymeth.2017.05.007_b0475","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1038\/nmeth.3400","article-title":"Correlated light and electron microscopy: ultrastructure lights up!","volume":"12","author":"de Boer","year":"2015","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ymeth.2017.05.007_b0480","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/B978-0-12-407239-8.00011-2","article-title":"Correlative microscopy","volume":"113","author":"Mironov","year":"2013","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0485","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1091\/mbc.e12-12-0863","article-title":"Conventional transmission electron microscopy","volume":"25","author":"Winey","year":"2014","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.ymeth.2017.05.007_b0490","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.virol.2010.06.043","article-title":"Three-dimensional structure of Rubella virus factories","volume":"405","author":"Fontana","year":"2010","journal-title":"Virology"},{"key":"10.1016\/j.ymeth.2017.05.007_b0495","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.1503191112","article-title":"Three-dimensional architecture of extended synaptotagmin-mediated endoplasmic reticulum-plasma membrane contact sites","author":"Fern\u00e1ndez-Busnadiego","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ymeth.2017.05.007_b0500","doi-asserted-by":"crossref","first-page":"3963","DOI":"10.1073\/pnas.0709530105","article-title":"Disclosure of the mycobacterial outer membrane: cryo-electron tomography and vitreous sections reveal the lipid bilayer structure","volume":"105","author":"Hoffmann","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ymeth.2017.05.007_b0505","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1111\/cmi.12468","article-title":"African swine fever virus assembles a single membrane derived from rupture of the endoplasmic reticulum","volume":"17","author":"Suarez","year":"2015","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0510","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1126\/science.aaf4594","article-title":"Microtubule doublets are double-track railways for intraflagellar transport trains","volume":"352","author":"Stepanek","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.ymeth.2017.05.007_b0515","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1038\/ncb2044","article-title":"Electron tomography reveals unbranched networks of actin filaments in lamellipodia","volume":"12","author":"Urban","year":"2010","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0520","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1126\/science.aad8857","article-title":"Visualizing the molecular sociology at the HeLa cell nuclear periphery","volume":"351","author":"Mahamid","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.ymeth.2017.05.007_b0525","doi-asserted-by":"crossref","first-page":"1877","DOI":"10.1002\/1873-3468.12153","article-title":"Correlative light and electron microscopy methods for the study of virus\u2013cell interactions","author":"Bykov","year":"2016","journal-title":"FEBS Lett."},{"key":"10.1016\/j.ymeth.2017.05.007_b0530","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1146\/annurev-virology-031413-085351","article-title":"Three-dimensional imaging of viral infections","volume":"1","author":"Risco","year":"2014","journal-title":"Ann. Rev. Virol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0535","doi-asserted-by":"crossref","first-page":"6316","DOI":"10.3390\/v7122940","article-title":"Viral infection at high magnification: 3D electron microscopy methods to analyze the architecture of infected cells","volume":"12","author":"Romero-Brey","year":"2015","journal-title":"Viruses"},{"key":"10.1016\/j.ymeth.2017.05.007_b0540","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1004374","article-title":"Reorganization of the endosomal system in salmonella-infected cells: the ultrastructure of salmonella-induced tubular compartments","volume":"10","author":"Krieger","year":"2014","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.ymeth.2017.05.007_b0545","doi-asserted-by":"crossref","first-page":"1886","DOI":"10.1080\/15548627.2016.1208888","article-title":"Intracellular Salmonella induces aggrephagy of host endomembranes in persistent infections","volume":"12","author":"Lopez-Montero","year":"2016","journal-title":"Autophagy"},{"key":"10.1016\/j.ymeth.2017.05.007_b0550","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1111\/cmi.12475","article-title":"The COPII complex and lysosomal VAMP7 determine intracellular Salmonella localization and growth","volume":"17","author":"Santos","year":"2015","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0555","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/978-1-4939-6673-8_11","article-title":"Bacterial pathogenesis: methods and protocols","volume":"1535","author":"Quereda","year":"2017","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0560","doi-asserted-by":"crossref","first-page":"1844","DOI":"10.4161\/auto.29992","article-title":"Correlative light and electron microscopy imaging of autophagy in a zebrafish infection model","volume":"10","author":"Hosseini","year":"2014","journal-title":"Autophagy"},{"key":"10.1016\/j.ymeth.2017.05.007_b0565","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.188433","article-title":"3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy","author":"Russell","year":"2016","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.ymeth.2017.05.007_b0570","first-page":"44","article-title":"Circular ruffle formation and closure lead to macropinocytosis in hepatocyte growth factor\/scatter factor-treated cells","volume":"61","author":"Dowrick","year":"1993","journal-title":"Eur. J. Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0575","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1242\/jcs.110.2.105","article-title":"Fluid-phase uptake by macropinocytosis in Dictyostelium","volume":"110","author":"Hacker","year":"1997","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.ymeth.2017.05.007_b0580","doi-asserted-by":"crossref","first-page":"2953","DOI":"10.1242\/jcs.115.14.2953","article-title":"Membrane ruffling and macropinocytosis in A431 cells require cholesterol","volume":"115","author":"Grimmer","year":"2002","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.ymeth.2017.05.007_b0585","doi-asserted-by":"crossref","first-page":"3967","DOI":"10.1242\/jcs.03167","article-title":"Visualisation of macropinosome maturation by the recruitment of sorting nexins","volume":"119","author":"Kerr","year":"2006","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.ymeth.2017.05.007_b0590","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.1242\/jcs.109.8.2005","article-title":"v-Src induces constitutive macropinocytosis in rat fibroblasts","volume":"109","author":"Veithen","year":"1996","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.ymeth.2017.05.007_b0595","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.ceb.2008.12.003","volume":"21","author":"Hoenger","year":"2009","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0600","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.jsb.2010.10.017","article-title":"Dual-axis electron tomography of biological specimens: extending the limits of specimen thickness with bright-field STEM imaging","volume":"174","author":"Sousa","year":"2011","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0605","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.jsb.2011.08.013","article-title":"Correlative 3D imaging of whole mammalian cells with light and electron microscopy","volume":"176","author":"Murphy","year":"2011","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0610","series-title":"Sample Preparation Handbook for Transmission Electron Microscopy: Methodology","first-page":"125","author":"Ayache","year":"2010"},{"key":"10.1016\/j.ymeth.2017.05.007_b0615","series-title":"Electron Microscopy: Methods and Protocols","first-page":"151","author":"Kaech","year":"2014"},{"key":"10.1016\/j.ymeth.2017.05.007_b0620","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1111\/j.1462-5822.2009.01415.x","article-title":"Galectin-3, a marker for vacuole lysis by invasive pathogens","volume":"12","author":"Paz","year":"2010","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.ymeth.2017.05.007_b0625","doi-asserted-by":"crossref","first-page":"4204","DOI":"10.1038\/emboj.2012.262","article-title":"Endocytic tubules regulated by Rab GTPases 5 and 11 are used for envelopment of herpes simplex virus","volume":"31","author":"Hollinshead","year":"2012","journal-title":"EMBO J."},{"key":"10.1016\/j.ymeth.2017.05.007_b0630","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/S0962-8924(98)01380-4","article-title":"Analysis of the endocytic system by using horseradish peroxidase","volume":"8","author":"Stoorvogel","year":"1998","journal-title":"Trends Cell Biol."}],"container-title":["Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202316304327?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202316304327?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T07:08:44Z","timestamp":1657264124000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046202316304327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8]]},"references-count":126,"alternative-id":["S1046202316304327"],"URL":"https:\/\/doi.org\/10.1016\/j.ymeth.2017.05.007","relation":{},"ISSN":["1046-2023"],"issn-type":[{"value":"1046-2023","type":"print"}],"subject":[],"published":{"date-parts":[[2017,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Imaging macropinosomes during Shigella infections","name":"articletitle","label":"Article Title"},{"value":"Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymeth.2017.05.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}