{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T07:44:30Z","timestamp":1767339870762,"version":"build-2065373602"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Methods"],"published-print":{"date-parts":[[2019,4]]},"DOI":"10.1016\/j.ymeth.2019.02.008","type":"journal-article","created":{"date-parts":[[2019,2,13]],"date-time":"2019-02-13T12:05:50Z","timestamp":1550059550000},"page":"17-21","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Use and reliability of multiplex bead-based assays (Luminex) at Containment Level 4"],"prefix":"10.1016","volume":"158","author":[{"given":"Stuart D.","family":"Dowall","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria A.","family":"Graham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Fletcher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Hewson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.ymeth.2019.02.008_b0005","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.ymeth.2012.06.001","article-title":"The pros and cons of multiplexing: a guest-editor's introduction","volume":"56","author":"Lewczuk","year":"2012","journal-title":"Methods"},{"issue":"3\u20134","key":"10.1016\/j.ymeth.2019.02.008_b0010","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1002\/prca.201400130","article-title":"ELISA in the multiplex era: potentials and pitfalls","volume":"9","author":"Tighe","year":"2015","journal-title":"Proteomics Clin. Appl."},{"issue":"4","key":"10.1016\/j.ymeth.2019.02.008_b0015","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.coi.2005.05.001","article-title":"Pathogenesis of viral hemorrhagic fever","volume":"17","author":"Bray","year":"2005","journal-title":"Curr. Opin. Immunol."},{"issue":"4","key":"10.1016\/j.ymeth.2019.02.008_b0020","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.ymeth.2005.11.008","article-title":"Solid-phase and bead-based cytokine immunoassay: a comparison","volume":"38","author":"de Jager","year":"2006","journal-title":"Methods"},{"issue":"4","key":"10.1016\/j.ymeth.2019.02.008_b0025","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.ymeth.2005.11.010","article-title":"Multiplex bead array assays: performance evaluation and comparison of sensitivity to ELISA","volume":"38","author":"Elshal","year":"2006","journal-title":"Methods"},{"issue":"1\u20132","key":"10.1016\/j.ymeth.2019.02.008_b0030","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.jim.2009.06.007","article-title":"Multiplex cytokine profiling with highly pathogenic material: use of formalin solution in luminex analysis","volume":"348","author":"Dowall","year":"2009","journal-title":"J. Immunol. Methods"},{"issue":"3","key":"10.1016\/j.ymeth.2019.02.008_b0035","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1002\/(SICI)1096-9071(199911)59:3<341::AID-JMV14>3.0.CO;2-C","article-title":"Cytokine measurement in biological samples after physicochemical treatment for inactivation of biosafety level 4 viral agents","volume":"59","author":"Mahanty","year":"1999","journal-title":"J. Med. Virol."},{"issue":"1","key":"10.1016\/j.ymeth.2019.02.008_b0040","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.antiviral.2013.07.006","article-title":"Crimean-Congo hemorrhagic fever: history, epidemiology, pathogenesis, clinical syndrome and genetic diversity","volume":"100","author":"Bente","year":"2013","journal-title":"Antiviral Res."},{"issue":"1","key":"10.1016\/j.ymeth.2019.02.008_b0045","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s12992-017-0233-9","article-title":"Global research trends of World Health Organization's top eight emerging pathogens","volume":"13","author":"Sweileh","year":"2017","journal-title":"Global Health"},{"issue":"44","key":"10.1016\/j.ymeth.2019.02.008_b0050","doi-asserted-by":"crossref","first-page":"6015","DOI":"10.1016\/j.vaccine.2017.05.031","article-title":"Development of vaccines against Crimean-Congo haemorrhagic fever virus","volume":"35","author":"Dowall","year":"2017","journal-title":"Vaccine"},{"issue":"1","key":"10.1016\/j.ymeth.2019.02.008_b0055","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1002\/jmv.24312","article-title":"Cytokines as biomarkers of Crimean-Congo hemorrhagic fever","volume":"88","author":"Papa","year":"2016","journal-title":"J. Med. Virol."},{"issue":"10","key":"10.1016\/j.ymeth.2019.02.008_b0060","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1002\/jmv.24864","article-title":"Cytokine response in crimean-congo hemorrhagic fever virus infection","volume":"89","author":"Ergonul","year":"2017","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.ymeth.2019.02.008_b0065","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1186\/1471-2334-14-416","article-title":"Sequential determination of serum viral titers, virus-specific IgG antibodies, and TNF-alpha, IL-6, IL-10, and IFN-gamma levels in patients with Crimean-Congo hemorrhagic fever","volume":"14","author":"Kaya","year":"2014","journal-title":"BMC Infect. Dis."},{"issue":"2","key":"10.1016\/j.ymeth.2019.02.008_b0070","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.virol.2011.11.027","article-title":"Serology and cytokine profiles in patients infected with the newly discovered Bundibugyo ebolavirus","volume":"423","author":"Gupta","year":"2012","journal-title":"Virology"},{"issue":"Suppl. 2","key":"10.1016\/j.ymeth.2019.02.008_b0075","doi-asserted-by":"crossref","first-page":"S357","DOI":"10.1086\/520611","article-title":"Cytokine and chemokine expression in humans infected with Sudan Ebola virus","volume":"196","author":"Hutchinson","year":"2007","journal-title":"J. Infect. Dis."},{"issue":"10","key":"10.1016\/j.ymeth.2019.02.008_b0080","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.3201\/eid2010.140430","article-title":"Biomarker correlates of survival in pediatric patients with Ebola virus disease","volume":"20","author":"McElroy","year":"2014","journal-title":"Emerg. Infect. Dis."},{"issue":"1\u20132","key":"10.1016\/j.ymeth.2019.02.008_b0085","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jim.2013.04.009","article-title":"Optimising the quantification of cytokines present at low concentrations in small human mucosal tissue samples using Luminex assays","volume":"394","author":"Staples","year":"2013","journal-title":"J. Immunol. Methods"},{"issue":"1","key":"10.1016\/j.ymeth.2019.02.008_b0090","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1002\/cyto.b.20021","article-title":"Multiplex bead array assays for detection of soluble cytokines: comparisons of sensitivity and quantitative values among kits from multiple manufacturers","volume":"61","author":"Khan","year":"2004","journal-title":"Cytometry B Clin. Cytom."},{"issue":"6","key":"10.1016\/j.ymeth.2019.02.008_b0095","first-page":"1235","article-title":"Use of coefficient of variation in assessing variability of quantitative assays","volume":"9","author":"Reed","year":"2002","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.ymeth.2019.02.008_b0100","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.euprot.2014.02.002","article-title":"The use of multiplex platforms for absolute and relative protein quantification of clinical material","volume":"3","author":"Christiansson","year":"2014","journal-title":"EuPA Open Proteom."},{"key":"10.1016\/j.ymeth.2019.02.008_b0105","first-page":"1151","article-title":"Human tear analysis with miniaturized multiplex cytokine assay on \u201cwall-less\u201d 96-well plate","volume":"21","author":"Le Guezennec","year":"2015","journal-title":"Mol. Vis."},{"issue":"12","key":"10.1016\/j.ymeth.2019.02.008_b0110","doi-asserted-by":"crossref","first-page":"4741","DOI":"10.1021\/ac702628q","article-title":"Biological and technical variables affecting immunoassay recovery of cytokines from human serum and simulated vaginal fluid: a multicenter study","volume":"80","author":"Fichorova","year":"2008","journal-title":"Anal. Chem."},{"issue":"4","key":"10.1016\/j.ymeth.2019.02.008_b0115","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0175130","article-title":"Validation of two multiplex platforms to quantify circulating markers of inflammation and endothelial injury in severe infection","volume":"12","author":"Leligdowicz","year":"2017","journal-title":"PLoS One"},{"issue":"13","key":"10.1016\/j.ymeth.2019.02.008_b0120","doi-asserted-by":"crossref","first-page":"8327","DOI":"10.1167\/iovs.13-12132","article-title":"Tear cytokine profile as a noninvasive biomarker of inflammation for ocular surface diseases: standard operating procedures","volume":"54","author":"Wei","year":"2013","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.ymeth.2019.02.008_b0125","unstructured":"Luminex. xMAP Cookbook. 3rd ed., [Available from: http:\/\/info.luminexcorp.com\/en-us\/research\/download-the-xmap-cookbook."},{"issue":"2","key":"10.1016\/j.ymeth.2019.02.008_b0130","doi-asserted-by":"crossref","first-page":"E156","DOI":"10.1208\/aapsj0902017","article-title":"Key elements of bioanalytical method validation for macromolecules","volume":"9","author":"Kelley","year":"2007","journal-title":"AAPS J."},{"issue":"10","key":"10.1016\/j.ymeth.2019.02.008_b0135","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.3346\/jkms.2017.32.10.1581","article-title":"Validation of the world health organization enzyme-linked immunosorbent assay for the quantitation of immunoglobulin g serotype-specific anti-pneumococcal antibodies in human serum","volume":"32","author":"Lee","year":"2017","journal-title":"J. Korean Med. Sci."},{"issue":"8","key":"10.1016\/j.ymeth.2019.02.008_b0140","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1128\/CVI.05032-11","article-title":"Multisite comparison of high-sensitivity multiplex cytokine assays","volume":"18","author":"Breen","year":"2011","journal-title":"Clin. Vaccine Immunol."},{"issue":"11","key":"10.1016\/j.ymeth.2019.02.008_b0145","doi-asserted-by":"crossref","first-page":"4655","DOI":"10.1002\/jps.22700","article-title":"The impact of decreased bead count to determine concentrations of amyloid beta1-42, total-tau, and phosphorylated-tau181 in human cerebrospinal fluid using xMAP technology","volume":"100","author":"Bjornstal","year":"2011","journal-title":"J. Pharm. Sci."},{"issue":"7","key":"10.1016\/j.ymeth.2019.02.008_b0150","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0002535","article-title":"An evaluation of commercial fluorescent bead-based luminex cytokine assays","volume":"3","author":"Djoba Siawaya","year":"2008","journal-title":"PLoS One"}],"container-title":["Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202318301725?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202318301725?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T01:33:40Z","timestamp":1760924020000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046202318301725"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4]]},"references-count":30,"alternative-id":["S1046202318301725"],"URL":"https:\/\/doi.org\/10.1016\/j.ymeth.2019.02.008","relation":{},"ISSN":["1046-2023"],"issn-type":[{"type":"print","value":"1046-2023"}],"subject":[],"published":{"date-parts":[[2019,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Use and reliability of multiplex bead-based assays (Luminex) at Containment Level 4","name":"articletitle","label":"Article Title"},{"value":"Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymeth.2019.02.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}