{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T11:01:01Z","timestamp":1768993261879,"version":"3.49.0"},"reference-count":65,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Government of Malaysia Ministry of Higher Education","doi-asserted-by":"publisher","award":["FRGS\/1\/2024\/STG01\/USM\/02\/10"],"award-info":[{"award-number":["FRGS\/1\/2024\/STG01\/USM\/02\/10"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Methods"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.ymeth.2025.12.005","type":"journal-article","created":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:13:53Z","timestamp":1765844033000},"page":"174-185","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Application of a 2A-peptide system for polycistronic human IgG production in Leishmania tarentolae"],"prefix":"10.1016","volume":"246","author":[{"given":"Jing Yi","family":"Lai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kae Yi","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Choo Hock","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yee Siew","family":"Choong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theam Soon","family":"Lim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"4705","key":"10.1016\/j.ymeth.2025.12.005_b0005","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1126\/science.4001944","article-title":"Filamentous fusion phage: Novel expression vectors that display cloned antigens on the virion surface","volume":"228","author":"Smith","year":"1985","journal-title":"Science"},{"issue":"1\u20133","key":"10.1016\/j.ymeth.2025.12.005_b0010","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/S0014-5793(02)02506-1","article-title":"A naturally occurring nar variable domain binds the kgp protease from porphyromonas gingivalis","volume":"516","author":"Nuttall","year":"2002","journal-title":"FEBS Lett."},{"issue":"2","key":"10.1016\/j.ymeth.2025.12.005_b0015","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/0378-1119(91)90244-6","article-title":"A surface expression vector for antibody screening","volume":"104","author":"Breitling","year":"1991","journal-title":"Gene"},{"issue":"26","key":"10.1016\/j.ymeth.2025.12.005_b0020","doi-asserted-by":"crossref","first-page":"18218","DOI":"10.1074\/jbc.274.26.18218","article-title":"A large non-immunized human fab fragment phage library that permits rapid isolation and kinetic analysis of high affinity antibodies","volume":"274","author":"de Haard","year":"1999","journal-title":"J. Biol. Chem."},{"issue":"4","key":"10.1016\/j.ymeth.2025.12.005_b0025","first-page":"233","article-title":"The global landscape of approved antibody therapies","volume":"5","author":"Lyu","year":"2022","journal-title":"Antib Ther"},{"issue":"12","key":"10.1016\/j.ymeth.2025.12.005_b0030","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1038\/s41587-022-01582-x","article-title":"Biopharmaceutical benchmarks 2022","volume":"40","author":"Walsh","year":"2022","journal-title":"Nat. Biotechnol."},{"issue":"8","key":"10.1016\/j.ymeth.2025.12.005_b0035","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1038\/s41590-023-01544-8","article-title":"Effect of posttranslational modifications and subclass on igg activity: from immunity to immunotherapy","volume":"24","author":"Nimmerjahn","year":"2023","journal-title":"Nat. Immunol."},{"issue":"5","key":"10.1016\/j.ymeth.2025.12.005_b0040","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/j.tibtech.2021.10.003","article-title":"Microbial protein cell factories fight back?","volume":"40","author":"Rettenbacher","year":"2022","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.ymeth.2025.12.005_b0045","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/2049-9957-4-8","article-title":"Leishmania tarentolae: an alternative approach to the production of monoclonal antibodies to treat emerging viral infections","volume":"4","author":"Jones","year":"2015","journal-title":"Infect. Dis. Poverty"},{"issue":"3","key":"10.1016\/j.ymeth.2025.12.005_b0050","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1080\/07388551.2019.1566206","article-title":"Potential application of leishmania tarentolae as an alternative platform for antibody expression","volume":"39","author":"Lai","year":"2019","journal-title":"Crit. Rev. Biotechnol."},{"issue":"2","key":"10.1016\/j.ymeth.2025.12.005_b0055","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S1046-5928(02)00001-3","article-title":"Non-pathogenic trypanosomatid protozoa as a platform for protein research and production","volume":"25","author":"Breitling","year":"2002","journal-title":"Protein Expr. Purif."},{"issue":"5","key":"10.1016\/j.ymeth.2025.12.005_b0060","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1016\/S0022-2836(02)01250-0","article-title":"Structural analysis of human igg-fc glycoforms reveals a correlation between glycosylation and structural integrity","volume":"325","author":"Krapp","year":"2003","journal-title":"J. Mol. Biol."},{"issue":"1","key":"10.1016\/j.ymeth.2025.12.005_b0065","doi-asserted-by":"crossref","first-page":"2193","DOI":"10.1038\/s41598-017-02460-2","article-title":"Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector","volume":"7","author":"Liu","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ymeth.2025.12.005_b0070","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/1475-2859-11-97","article-title":"Secretory signal peptide modification for optimized antibody-fragment expression-secretion in leishmania tarentolae","volume":"11","author":"Klatt","year":"2012","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.ymeth.2025.12.005_b0075","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1475-2859-13-9","article-title":"Expression of single-chain variable fragments fused with the fc-region of rabbit igg in leishmania tarentolae","volume":"13","author":"J\u00f8rgensen","year":"2014","journal-title":"Microb. Cell Fact."},{"issue":"6","key":"10.1016\/j.ymeth.2025.12.005_b0080","doi-asserted-by":"crossref","DOI":"10.1111\/micc.12365","article-title":"Pericyte modulation by a functional antibody obtained by a novel single-cell selection strategy","volume":"24","author":"Just","year":"2017","journal-title":"Microcirculation"},{"issue":"1","key":"10.1016\/j.ymeth.2025.12.005_b0085","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/s10989-020-10088-6","article-title":"Expression, purification, and evaluation of anti-il-2r\u03b1 antibody secreted by leishmania tarentolae","volume":"27","author":"Jalali","year":"2021","journal-title":"Int. J. Pept. Res. Ther."},{"issue":"6","key":"10.1016\/j.ymeth.2025.12.005_b0090","doi-asserted-by":"crossref","first-page":"2907","DOI":"10.1007\/s11033-018-4351-0","article-title":"Construction strategies for developing expression vectors for recombinant monoclonal antibody production in cho cells","volume":"45","author":"Li","year":"2018","journal-title":"Mol. Biol. Rep."},{"issue":"1","key":"10.1016\/j.ymeth.2025.12.005_b0095","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1021\/bp049780w","article-title":"On the optimal ratio of heavy to light chain genes for efficient recombinant antibody production by cho cells","volume":"21","author":"Schlatter","year":"2005","journal-title":"Biotechnol. Prog."},{"issue":"1\u20132","key":"10.1016\/j.ymeth.2025.12.005_b0100","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.jim.2006.10.010","article-title":"A comparative study of different vector designs for the mammalian expression of recombinant igg antibodies","volume":"318","author":"Li","year":"2007","journal-title":"J. Immunol. Methods"},{"issue":"2","key":"10.1016\/j.ymeth.2025.12.005_b0105","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1080\/19420862.2015.1008351","article-title":"Cleavage efficient 2a peptides for high level monoclonal antibody expression in cho cells","volume":"7","author":"Chng","year":"2015","journal-title":"MAbs"},{"issue":"5","key":"10.1016\/j.ymeth.2025.12.005_b0110","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0063247","article-title":"Comparison of internal ribosome entry site (ires) and furin-2a (f2a) for monoclonal antibody expression level and quality in cho cells","volume":"8","author":"Ho","year":"2013","journal-title":"PLoS One"},{"issue":"11","key":"10.1016\/j.ymeth.2025.12.005_b0115","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.1099\/0022-1317-72-11-2727","article-title":"Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence","volume":"72","author":"Ryan","year":"1991","journal-title":"J. Gen. Virol."},{"issue":"Pt 5","key":"10.1016\/j.ymeth.2025.12.005_b0120","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1099\/0022-1317-82-5-1013","article-title":"Analysis of the aphthovirus 2a\/2b polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip'","volume":"82","author":"Donnelly","year":"2001","journal-title":"J. Gen. Virol."},{"issue":"4","key":"10.1016\/j.ymeth.2025.12.005_b0125","doi-asserted-by":"crossref","DOI":"10.1002\/j.1460-2075.1994.tb06337.x","article-title":"Foot\u2010and\u2010mouth disease virus 2a oligopeptide mediated cleavage of an artificial polyprotein","volume":"13","author":"Ryan","year":"1994","journal-title":"EMBO J."},{"issue":"5","key":"10.1016\/j.ymeth.2025.12.005_b0130","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1517\/14712598.5.5.627","article-title":"Development of 2a peptide-based strategies in the design of multicistronic vectors","volume":"5","author":"Szymczak","year":"2005","journal-title":"Expert Opin. Biol. Ther."},{"key":"10.1016\/j.ymeth.2025.12.005_b0135","series-title":"Snake Venoms","first-page":"215","article-title":"Snake \u03b1-neurotoxins and the nicotinic acetylcholine receptor","author":"Nirthanan","year":"2017"},{"key":"10.1016\/j.ymeth.2025.12.005_b0140","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s40409-017-0127-6","article-title":"Snakebite envenomation turns again into a neglected tropical disease!","volume":"23","author":"Chippaux","year":"2017","journal-title":"J. Venom. Anim. Toxins. Incl. Trop. Dis."},{"key":"10.1016\/j.ymeth.2025.12.005_b0145","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.661457","article-title":"Snakebite envenoming diagnosis and diagnostics","volume":"12","author":"Knudsen","year":"2021","journal-title":"Front. Immunol."},{"issue":"9\u201310","key":"10.1016\/j.ymeth.2025.12.005_b0150","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1002\/prca.201600002","article-title":"Recombinant antibodies for diagnostics and therapy against pathogens and toxins generated by phage display","volume":"10","author":"Kuhn","year":"2016","journal-title":"Proteomics Clin. Appl."},{"issue":"4","key":"10.1016\/j.ymeth.2025.12.005_b0155","doi-asserted-by":"crossref","first-page":"86","DOI":"10.3390\/toxins8040086","article-title":"Neutralization of the principal toxins from the venoms of thai naja kaouthia and malaysian hydrophis schistosus: insights into toxin-specific neutralization by two different antivenoms","volume":"8","author":"Tan","year":"2016","journal-title":"Toxins (Basel)"},{"key":"10.1016\/j.ymeth.2025.12.005_b0160","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.toxicon.2016.04.032","article-title":"Generation of a na\u00efve human single chain variable fragment (scfv) library for the identification of monoclonal scfv against salmonella typhi hemolysin e antigen","volume":"117","author":"Lim","year":"2016","journal-title":"Toxicon"},{"key":"10.1016\/j.ymeth.2025.12.005_b0165","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2023.125571","article-title":"Na\u00efve antibody library derived monoclonal antibody against vp35 of ebola virus","volume":"245","author":"Lai","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.ymeth.2025.12.005_b0170","doi-asserted-by":"crossref","unstructured":"X. Brochet, M.P. Lefranc, V. Giudicelli, Imgt\/v-quest: The highly customized and integrated system for ig and tr standardized v-j and v-d-j sequence analysis, Nucleic Acids Res 36(Web Server issue) (2008) W503-508.","DOI":"10.1093\/nar\/gkn316"},{"issue":"6","key":"10.1016\/j.ymeth.2025.12.005_b0175","first-page":"695","article-title":"Imgt\/v-quest: Imgt standardized analysis of the immunoglobulin (ig) and t cell receptor (tr) nucleotide sequences","volume":"2011","author":"Giudicelli","year":"2011","journal-title":"Cold Spring Harb. Protoc."},{"issue":"1","key":"10.1016\/j.ymeth.2025.12.005_b0180","doi-asserted-by":"crossref","first-page":"2176","DOI":"10.1038\/s41598-017-01987-8","article-title":"Delineation of b-cell epitopes of salmonella enterica serovar typhi hemolysin e: potential antibody therapeutic target","volume":"7","author":"Chin","year":"2017","journal-title":"Sci. Rep."},{"issue":"7","key":"10.1016\/j.ymeth.2025.12.005_b0185","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1038\/s41587-021-01156-3","article-title":"Signalp 6.0 predicts all five types of signal peptides using protein language models","volume":"40","author":"Teufel","year":"2022","journal-title":"Nat. Biotechnol."},{"issue":"7537","key":"10.1016\/j.ymeth.2025.12.005_b0190","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1038\/518027a","article-title":"Reproducibility: standardize antibodies used in research","volume":"518","author":"Bradbury","year":"2015","journal-title":"Nature"},{"key":"10.1016\/j.ymeth.2025.12.005_b0195","doi-asserted-by":"crossref","first-page":"1986","DOI":"10.3389\/fimmu.2020.01986","article-title":"Phage display derived monoclonal antibodies: from bench to bedside","volume":"11","author":"Alfaleh","year":"2020","journal-title":"Front. Immunol."},{"issue":"3","key":"10.1016\/j.ymeth.2025.12.005_b0200","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1038\/nbt0396-309","article-title":"Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library","volume":"14","author":"Vaughan","year":"1996","journal-title":"Nat. Biotechnol."},{"issue":"4","key":"10.1016\/j.ymeth.2025.12.005_b0205","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1046\/j.1365-3083.2000.00709.x","article-title":"Neutralizing human anti crotoxin scfv isolated from a nonimmunized phage library","volume":"51","author":"Cardoso","year":"2000","journal-title":"Scand. J. Immunol."},{"issue":"10","key":"10.1016\/j.ymeth.2025.12.005_b0210","doi-asserted-by":"crossref","first-page":"2591","DOI":"10.1111\/j.1742-4658.2005.04687.x","article-title":"A strategy for the generation of specific human antibodies by directed evolution and phage display","volume":"272","author":"Ria\u00f1o-Umbarila","year":"2005","journal-title":"FEBS J."},{"issue":"2","key":"10.1016\/j.ymeth.2025.12.005_b0215","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1111\/j.1742-7843.2008.00322.x","article-title":"Expression of human recombinant antibody fragments capable of partially inhibiting the phospholypase activity of crotalus durissus terrificus venom","volume":"105","author":"Oliveira","year":"2009","journal-title":"Basic Clin. Pharmacol. Toxicol."},{"issue":"2","key":"10.1016\/j.ymeth.2025.12.005_b0220","doi-asserted-by":"crossref","first-page":"173","DOI":"10.3109\/1547691X.2011.649220","article-title":"Serrumab: a human monoclonal antibody that counters the biochemical and immunological effects of tityus serrulatus venom","volume":"9","author":"Pucca","year":"2012","journal-title":"J. Immunotoxicol."},{"issue":"3","key":"10.1016\/j.ymeth.2025.12.005_b0225","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1111\/j.1742-7843.2011.00821.x","article-title":"Production of human antibody fragments binding to melittin and phospholipase a2 in africanised bee venom: Minimising venom toxicity","volume":"110","author":"Funayama","year":"2012","journal-title":"Basic Clin. Pharmacol. Toxicol."},{"issue":"1","key":"10.1016\/j.ymeth.2025.12.005_b0230","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1038\/s41467-023-36393-4","article-title":"Discovery and optimization of a broadly-neutralizing human monoclonal antibody against long-chain \u03b1-neurotoxins from snakes","volume":"14","author":"Ledsgaard","year":"2023","journal-title":"Nat. Commun."},{"issue":"2","key":"10.1016\/j.ymeth.2025.12.005_b0235","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.jprot.2008.12.007","article-title":"Human monoclonal scfv neutralize lethal thai cobra, naja kaouthia, neurotoxin","volume":"72","author":"Kulkeaw","year":"2009","journal-title":"J. Proteomics"},{"issue":"1","key":"10.1016\/j.ymeth.2025.12.005_b0240","doi-asserted-by":"crossref","DOI":"10.1080\/19420862.2022.2085536","article-title":"In vitro discovery of a human monoclonal antibody that neutralizes lethality of cobra snake venom","volume":"14","author":"Ledsgaard","year":"2022","journal-title":"MAbs"},{"issue":"4","key":"10.1016\/j.ymeth.2025.12.005_b0245","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1038\/s41435-021-00145-5","article-title":"Immunoglobulin germline gene variation and its impact on human disease","volume":"22","author":"Mikocziova","year":"2021","journal-title":"Genes Immunity"},{"issue":"10","key":"10.1016\/j.ymeth.2025.12.005_b0250","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2023.113194","article-title":"Widespread impact of immunoglobulin v-gene allelic polymorphisms on antibody reactivity","volume":"42","author":"Yuan","year":"2023","journal-title":"Cell Rep."},{"issue":"4","key":"10.1016\/j.ymeth.2025.12.005_b0255","doi-asserted-by":"crossref","first-page":"445","DOI":"10.4161\/mabs.20776","article-title":"Molecular engineering of antibodies for therapeutic and diagnostic purposes","volume":"4","author":"Ducancel","year":"2012","journal-title":"MAbs"},{"key":"10.1016\/j.ymeth.2025.12.005_b0260","article-title":"Factors affecting the expression of recombinant protein and improvement strategies in Chinese hamster ovary cells","volume":"10","author":"Li","year":"2022","journal-title":"Front. Bioeng. Biotechnol."},{"issue":"1","key":"10.1016\/j.ymeth.2025.12.005_b0265","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1021\/pr300693f","article-title":"Production of glycosylated soluble amyloid precursor protein alpha (sappalpha) in leishmania tarentolae","volume":"12","author":"Klatt","year":"2013","journal-title":"J. Proteome Res."},{"issue":"4","key":"10.1016\/j.ymeth.2025.12.005_b0270","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0018556","article-title":"High cleavage efficiency of a 2a peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice","volume":"6","author":"Kim","year":"2011","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.ymeth.2025.12.005_b0275","doi-asserted-by":"crossref","first-page":"16273","DOI":"10.1038\/srep16273","article-title":"2a self-cleaving peptide-based multi-gene expression system in the silkworm bombyx mori","volume":"5","author":"Wang","year":"2015","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.ymeth.2025.12.005_b0280","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.synbio.2024.09.010","article-title":"Developing polycistronic expression tool in Yarrowia lipolytica","volume":"10","author":"Li","year":"2024","journal-title":"Synth. Syst. Biotechnol."},{"issue":"1","key":"10.1016\/j.ymeth.2025.12.005_b0285","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.ab.2009.11.032","article-title":"Signal sequence is still required in genes downstream of \u201cautocleaving\u201d 2a peptide for secretary or membrane-anchored expression","volume":"399","author":"Yan","year":"2010","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ymeth.2025.12.005_b0290","article-title":"Expression of recombinant antibodies","volume":"4\u20132013","author":"Frenzel","year":"2013","journal-title":"Front. Immunol."},{"key":"10.1016\/j.ymeth.2025.12.005_b0295","article-title":"Factors affecting the expression of recombinant protein and improvement strategies in Chinese hamster ovary cells","volume":"10","author":"Li","year":"2022","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.ymeth.2025.12.005_b0300","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1080\/02648725.2007.10648095","article-title":"Dynamic light scattering as a relative tool for assessing the molecular integrity and stability of monoclonal antibodies","volume":"24","author":"Nobbmann","year":"2007","journal-title":"Biotechnol. Genet. Eng. Rev."},{"key":"10.1016\/j.ymeth.2025.12.005_b0305","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1080\/19420862.2017.1368602","article-title":"Forced degradation of recombinant monoclonal antibodies: a practical guide","volume":"9","author":"Nowak","year":"2017","journal-title":"mAbs"},{"issue":"17","key":"10.1016\/j.ymeth.2025.12.005_b0310","doi-asserted-by":"crossref","first-page":"5404","DOI":"10.1021\/acssuschemeng.1c07979","article-title":"Cholinium-based ionic liquids as efficient media for improving the structural and thermal stability of immunoglobulin g antibodies","volume":"10","author":"Dhiman","year":"2022","journal-title":"ACS Sustain. Chem. Eng."},{"issue":"5","key":"10.1016\/j.ymeth.2025.12.005_b0315","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.4161\/mabs.29680","article-title":"A general approach to antibody thermostabilization","volume":"6","author":"McConnell","year":"2014","journal-title":"MAbs"},{"issue":"4","key":"10.1016\/j.ymeth.2025.12.005_b0320","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1080\/19420862.2018.1450126","article-title":"Modification of the kinetic stability of immunoglobulin g by solvent additives","volume":"10","author":"Schaefer","year":"2018","journal-title":"MAbs"},{"issue":"1","key":"10.1016\/j.ymeth.2025.12.005_b0325","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.biologicals.2005.06.007","article-title":"The effect of solvent environment on the conformation and stability of human polyclonal igg in solution","volume":"34","author":"Szenczi","year":"2006","journal-title":"Biologicals"}],"container-title":["Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202325002488?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202325002488?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T21:06:57Z","timestamp":1768943217000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046202325002488"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":65,"alternative-id":["S1046202325002488"],"URL":"https:\/\/doi.org\/10.1016\/j.ymeth.2025.12.005","relation":{},"ISSN":["1046-2023"],"issn-type":[{"value":"1046-2023","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Application of a 2A-peptide system for polycistronic human IgG production in Leishmania tarentolae","name":"articletitle","label":"Article Title"},{"value":"Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymeth.2025.12.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}