{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T13:11:23Z","timestamp":1776172283598,"version":"3.50.1"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100031027","name":"ICAR-Indian Institute of Horticultural Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100031027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Methods"],"published-print":{"date-parts":[[2026,5]]},"DOI":"10.1016\/j.ymeth.2026.02.010","type":"journal-article","created":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T20:09:53Z","timestamp":1771358993000},"page":"9-22","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["First report of CRISPR\/Cas13a-based rapid detection of groundnut bud necrosis virus without amplification"],"prefix":"10.1016","volume":"249","author":[{"given":"Thalooru","family":"Shashikala","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dhawane","family":"Yogi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumar","family":"Akshay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somakalahalli Narasimhappa","family":"Nagesh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maligeppagol","family":"Manamohan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Venkataravanappa","family":"Venkataravanappa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Girish Kumar","family":"Jha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karuppannasamy","family":"Ashok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramasamy","family":"Asokan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymeth.2026.02.010_b0090","article-title":"Tomatoes unveiled: a comprehensive exploration from cultivation to culinary and nutritional significance","author":"Sattar","year":"2024","journal-title":"Qeios."},{"key":"10.1016\/j.ymeth.2026.02.010_b0095","unstructured":"APEDA. 2021. Export statistics for tomatoes. Agricultural and Processed Food Products Export Development Authority."},{"key":"10.1016\/j.ymeth.2026.02.010_b0100","series-title":"Food Processing in India: Opportunities in the Tomato Value Chain","author":"NABARD","year":"2020"},{"key":"10.1016\/j.ymeth.2026.02.010_b0075","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/j.1744-7348.2003.tb00240.x","article-title":"Emerging geminivirus problems: a serious threat to crop production","volume":"142","author":"Varma","year":"2003","journal-title":"Ann. Appl. Biol."},{"key":"10.1016\/j.ymeth.2026.02.010_b0195","series-title":"Applied Plant Virology","first-page":"531","article-title":"Overview of yield losses due to plant viruses","author":"Rao","year":"2020"},{"key":"10.1016\/j.ymeth.2026.02.010_b0085","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/j.tree.2004.07.021","article-title":"Emerging infectious diseases of plants: Pathogen pollution, climate change and agrotechnology drivers","volume":"19","author":"Anderson","year":"2004","journal-title":"Trends Ecol. Evol."},{"key":"10.1016\/j.ymeth.2026.02.010_b0110","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.virusres.2009.01.009","article-title":"Global status of tospovirus epidemics in diverse cropping systems: successes achieved and challenges ahead","volume":"141","author":"Pappu","year":"2009","journal-title":"Virus Res."},{"key":"10.1016\/j.ymeth.2026.02.010_b0200","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1046\/j.1364-3703.2000.00022.x","article-title":"Tomato spotted wilt virus\u2014positive steps towards negative success","volume":"1","author":"Adkins","year":"2000","journal-title":"Mol. Plant Pathol"},{"key":"10.1016\/j.ymeth.2026.02.010_b0205","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1006\/viro.1994.1162","article-title":"Expression and subcellular location of the NSM protein of tomato spotted wilt virus (TSWV), a putative viral movement protein","volume":"200","author":"Kormelink","year":"1994","journal-title":"Virology"},{"key":"10.1016\/j.ymeth.2026.02.010_b0005","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1094\/PHYTO-08-16-0296-R","article-title":"Influence of groundnut bud necrosis virus on the life history traits and feeding preference of its vector, Thrips palmi","volume":"107","author":"Daimei","year":"2017","journal-title":"Phytopathology"},{"key":"10.1016\/j.ymeth.2026.02.010_b0175","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1111\/j.1744-7348.1992.tb03425.x","article-title":"Serological relationships and purification of bud necrosis virus, a tospovirus occurring in peanut (Arachis hypogaea L.) in India","volume":"120","author":"Reddy","year":"1992","journal-title":"Ann. Appl. Biol."},{"key":"10.1016\/j.ymeth.2026.02.010_b0010","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/s007050050291","article-title":"Peanut yellow spot virus is a member of a new serogroup of tospovirus genus based on small (S) RNA sequence and organization","volume":"143","author":"Satyanarayana","year":"1998","journal-title":"Arch. Virol."},{"key":"10.1016\/j.ymeth.2026.02.010_b0210","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1111\/j.1744-7348.1979.tb06528.x","article-title":"Bud necrosis of groundnut (Arachis hypogaea) in India caused by tomato spotted wilt virus","volume":"93","author":"Ghanekar","year":"1979","journal-title":"Ann. Appl. Biol."},{"key":"10.1016\/j.ymeth.2026.02.010_b0220","first-page":"9","article-title":"Tomato spotted wilt virus as the causal pathogen of leaf curl of mungbean, Vigna radiata (L.) Wilczek and urdbean, Vigna mungo (L.) Hepper in Andhra Pradesh","volume":"13","author":"Amin","year":"1985","journal-title":"Indian Journal of Plant Protection"},{"key":"10.1016\/j.ymeth.2026.02.010_b0225","first-page":"1389","article-title":"Nucleocapsid protein gene sequence studies suggest that soybean bud blight is caused by a strain of groundnut bud necrosis virus","volume":"82","author":"Bhat","year":"2002","journal-title":"Curr. Sci."},{"key":"10.1016\/j.ymeth.2026.02.010_b0070","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1146\/annurev-phyto-082712-102346","article-title":"Status and prospects of plant virus control through interference with vector transmission","volume":"51","author":"Bragard","year":"2013","journal-title":"Annu. Rev. Phytopathol."},{"key":"10.1016\/j.ymeth.2026.02.010_b0080","doi-asserted-by":"crossref","first-page":"173","DOI":"10.5423\/RPD.2014.20.3.173","article-title":"A review of detection methods for the plant viruses","volume":"20","author":"Jeong","year":"2014","journal-title":"Res. Plant Dis."},{"key":"10.1016\/j.ymeth.2026.02.010_b0020","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.jviromet.2010.04.024","article-title":"Evaluation of several RT-PCR primer pairs for the detection of Apple stem pitting virus","volume":"168","author":"Komorowska","year":"2010","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.ymeth.2026.02.010_b0035","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0166-0934(01)00381-0","article-title":"Detection of four apple viruses by multiplex RT-PCR assays with co-amplification of plant mRNA as internal control","volume":"99","author":"Menzel","year":"2002","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.ymeth.2026.02.010_b0015","doi-asserted-by":"crossref","first-page":"575","DOI":"10.5423\/PPJ.NT.06.2018.0108","article-title":"Rapid and specific detection of apple stem grooving virus by reverse transcription recombinase polymerase amplification","volume":"34","author":"Kim","year":"2018","journal-title":"Plant Pathol. J."},{"key":"10.1016\/j.ymeth.2026.02.010_b0050","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1007\/s10658-014-0397-6","article-title":"Rapid detection of Apple stem grooving virus by reverse transcription loop-mediated isothermal amplification","volume":"96","author":"Zhao","year":"2014","journal-title":"J. Plant Pathol."},{"key":"10.1016\/j.ymeth.2026.02.010_b0040","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1111\/j.1365-2672.2012.05384.x","article-title":"PCR inhibitors: occurrence, properties and removal","volume":"113","author":"Schrader","year":"2012","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.ymeth.2026.02.010_b0185","doi-asserted-by":"crossref","first-page":"412","DOI":"10.3390\/v13030412","article-title":"Current developments and challenges in plant viral diagnostics: a systematic review","volume":"13","author":"Mehetre","year":"2021","journal-title":"Viruses"},{"issue":"10","key":"10.1016\/j.ymeth.2026.02.010_b0250","doi-asserted-by":"crossref","first-page":"2986","DOI":"10.1038\/s41596-019-0210-2","article-title":"SHERLOCK: nucleic acid detection with CRISPR nucleases","volume":"14","author":"Kellner","year":"2019","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ymeth.2026.02.010_b0025","doi-asserted-by":"crossref","first-page":"38896","DOI":"10.1038\/srep38896","article-title":"Specific and sensitive isothermal electrochemical biosensor for plant pathogen DNA detection with colloidal gold nanoparticles as probes","volume":"7","author":"Lau","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ymeth.2026.02.010_b0045","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/978-1-61779-609-8_13","article-title":"Loop-mediated isothermal amplification for the detection of plant pathogens","volume":"862","author":"Ward","year":"2012","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.ymeth.2026.02.010_b0060","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.aar6245","article-title":"CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.ymeth.2026.02.010_b0120","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1038\/s41587-020-0513-4","article-title":"CRISPR\u2013Cas12-based detection of SARS-CoV-2","volume":"38","author":"Broughton","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.ymeth.2026.02.010_b0190","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1080\/22221751.2020.1763857","article-title":"CRISPR-based platform for carbapenemases and emerging viruses detection using Cas12a (Cpf1) effector nuclease","volume":"9","author":"Curti","year":"2020","journal-title":"Emerging Microbes Infect."},{"key":"10.1016\/j.ymeth.2026.02.010_b0055","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1126\/science.aam9321","article-title":"Nucleic acid detection with CRISPR-Cas13a\/C2c2","volume":"356","author":"Gootenberg","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.ymeth.2026.02.010_b0125","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1038\/nature24049","article-title":"RNA targeting with CRISPR\u2013Cas13","volume":"550","author":"Abudayyeh","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.ymeth.2026.02.010_b0065","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1126\/science.aav4294","article-title":"Programmed DNA destruction by miniature CRISPR-Cas14 enzymes","volume":"362","author":"Harrington","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.ymeth.2026.02.010_b0130","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1089\/crispr.2019.0011","article-title":"Nucleic acid detection of plant genes using CRISPR-Cas13","volume":"2","author":"Abudayyeh","year":"2019","journal-title":"CRISPR Journal"},{"issue":"3","key":"10.1016\/j.ymeth.2026.02.010_b0230","first-page":"254","article-title":"A protocol for detection of plant RNA viruses using CRISPR-based SHERLOCK","volume":"36","author":"Zhang","year":"2018","journal-title":"Nat. Protoc."},{"issue":"7624","key":"10.1016\/j.ymeth.2026.02.010_b0235","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/nature19802","article-title":"Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection","volume":"538","author":"East-Seletsky","year":"2016","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.ymeth.2026.02.010_b0240","doi-asserted-by":"crossref","first-page":"2825","DOI":"10.1038\/s41598-022-06810-7","article-title":"An optimized method to obtain high-quality RNA from different tissues in Lilium davidii var. unicolor","volume":"12","author":"Wang","year":"2022","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.ymeth.2026.02.010_b0245","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/s41589-022-01135-y","article-title":"Engineered LwaCas13a with enhanced collateral activity for nucleic acid detection","volume":"19","author":"Yang","year":"2023","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.ymeth.2026.02.010_b0155","doi-asserted-by":"crossref","first-page":"2384","DOI":"10.1021\/acssynbio.2c00090","article-title":"Diagnostics of infections produced by the plant viruses TMV, TEV, and PVX with CRISPR-Cas12 and CRISPR-Cas13","volume":"11","author":"Marqu\u00e9s","year":"2022","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.ymeth.2026.02.010_b0150","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1016\/j.molcel.2019.09.013","article-title":"Programmable inhibition and detection of RNA viruses using Cas13","volume":"76","author":"Freije","year":"2019","journal-title":"Mol. Cell"},{"key":"10.1016\/j.ymeth.2026.02.010_b0160","doi-asserted-by":"crossref","unstructured":"Metsky, H. C., C. A. Freije, T.-S. F. Kosoko-Thoroddsen, P. C. Sabeti, and C. Myhrvold. 2020. CRISPR-based surveillance for COVID-19 using genomically-comprehensive machine learning design. bioRxiv. https:\/\/doi.org\/10.1101\/2020.02.26.967026.","DOI":"10.1101\/2020.02.26.967026"},{"key":"10.1016\/j.ymeth.2026.02.010_b0165","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1126\/science.aas8836","article-title":"Field-deployable viral diagnostics using CRISPR-Cas13","volume":"360","author":"Myhrvold","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.ymeth.2026.02.010_b0135","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1186\/s13059-019-1881-2","article-title":"CRISPR-Cas13d mediates robust RNA virus interference in plants","volume":"20","author":"Mahas","year":"2019","journal-title":"Genome Biol."},{"key":"10.1016\/j.ymeth.2026.02.010_b0140","article-title":"Rapid detection of Tomato spotted wilt virus with Cas13a in tomato and Frankliniella occidentalis","volume":"12","author":"Zhang","year":"2021","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ymeth.2026.02.010_b0145","doi-asserted-by":"crossref","unstructured":"Hak, H., S. Ostendorp, A. Reza, S. Ishgur Greenberg, G. Pines, J. Kehr, and Z. Spiegelman. 2024. Rapid, direct, and sequence-specific identification of RNA viruses in various crop plants using CRISPR\/Cas13a. bioRxiv: 2024-02. https:\/\/doi.org\/10.1101\/2024.02.22.581525.","DOI":"10.1101\/2024.02.22.581525"},{"key":"10.1016\/j.ymeth.2026.02.010_b0170","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.3390\/v16071079","article-title":"Development of a CRISPR\/SHERLOCK-based method for rapid and sensitive detection of selected pospiviroids","volume":"16","author":"Zhai","year":"2024","journal-title":"Viruses"}],"container-title":["Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202326000344?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202326000344?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T12:36:06Z","timestamp":1776170166000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046202326000344"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":45,"alternative-id":["S1046202326000344"],"URL":"https:\/\/doi.org\/10.1016\/j.ymeth.2026.02.010","relation":{},"ISSN":["1046-2023"],"issn-type":[{"value":"1046-2023","type":"print"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"First report of CRISPR\/Cas13a-based rapid detection of groundnut bud necrosis virus without amplification","name":"articletitle","label":"Article Title"},{"value":"Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymeth.2026.02.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}