{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T22:26:15Z","timestamp":1778797575367,"version":"3.51.4"},"reference-count":76,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence nationale de la recherche","doi-asserted-by":"publisher","award":["ANR-20-CHIN-0001"],"award-info":[{"award-number":["ANR-20-CHIN-0001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007175","name":"Conseil National de la Recherche Scientifique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007175","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018694","name":"Universit\u00e9 de Pau et des Pays de l&apos;Adour","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018694","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Methods"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.ymeth.2026.04.003","type":"journal-article","created":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T15:09:56Z","timestamp":1776352196000},"page":"113-125","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Under the microscope: microbial life at pore scale under extreme deep-environment conditions"],"prefix":"10.1016","volume":"251","author":[{"given":"Saphir","family":"Venet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marion","family":"Guignard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magali","family":"Ranchou-Peyruse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrice","family":"Bordat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy","family":"Vallet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Dambrine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony","family":"Ranchou-Peyruse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ross","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.ymeth.2026.04.003_b0005","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1038\/scientificamerican1096-68","article-title":"Microbes deep inside the earth","volume":"275","author":"Fredrickson","year":"1996","journal-title":"Sci. Am."},{"key":"10.1016\/j.ymeth.2026.04.003_b0010","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1038\/s41561-018-0221-6","article-title":"The biomass and biodiversity of the continental subsurface","volume":"11","author":"Magnabosco","year":"2018","journal-title":"Nat. Geosci."},{"key":"10.1016\/j.ymeth.2026.04.003_b0015","doi-asserted-by":"crossref","unstructured":"T. Stevens, Subsurface microbiology and the evolution of the biosphere, in: Microbiology of the Terrestrial Deep Subsurface, CRC Press, 2018, pp. 205-224.","DOI":"10.1201\/9781351074568-12"},{"key":"10.1016\/j.ymeth.2026.04.003_b0020","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1038\/s41467-020-15064-8","article-title":"Fresh groundwater discharge insignificant for the world's oceans but important for coastal ecosystems","volume":"11","author":"Luijendijk","year":"2020","journal-title":"Nat. Comm."},{"key":"10.1016\/j.ymeth.2026.04.003_b0025","doi-asserted-by":"crossref","unstructured":"L. Purkamo Life in the deep subsurface and its implications for astrobiology (06 2023 10.1093\/acrefore\/9780190647926.013.274.","DOI":"10.1093\/acrefore\/9780190647926.013.274"},{"key":"10.1016\/j.ymeth.2026.04.003_b0030","doi-asserted-by":"crossref","first-page":"4631","DOI":"10.1073\/pnas.0400522101","article-title":"Temperature dependence of metabolic rates for microbial growth, maintenance, and survival","volume":"101","author":"Price","year":"2004","journal-title":"Proc. Nat. Acad. Sci."},{"key":"10.1016\/j.ymeth.2026.04.003_b0035","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1038\/nrmicro2939","article-title":"Microbial life under extreme energy limitation","volume":"11","author":"Hoehler","year":"2013","journal-title":"Nat. Rev. Microbiol."},{"issue":"1","key":"10.1016\/j.ymeth.2026.04.003_b0040","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1007\/s00248-024-02434-8","article-title":"Subsurface microbial colonization at mineral-filled veins in 2-billion-year-old mafic rock from the bushveld igneous complex, South Africa","volume":"87","author":"Suzuki","year":"2024","journal-title":"Microb. Ecol."},{"key":"10.1016\/j.ymeth.2026.04.003_b0045","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0065-2164(08)00806-X","article-title":"Microbial processes in oil fields: culprits, problems, and opportunities","volume":"66","author":"Youssef","year":"2009","journal-title":"Adv. Appl. Microbiol."},{"issue":"7","key":"10.1016\/j.ymeth.2026.04.003_b0050","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1038\/ismej.2007.75","article-title":"The geomicrobiology of gold","volume":"1","author":"Reith","year":"2007","journal-title":"ISME J."},{"key":"10.1016\/j.ymeth.2026.04.003_b0055","first-page":"279","article-title":"Microbial processes in the disposal of high level radioactive waste 500 m underground in fennoscandian shield rocks","volume":"Vol. 2","author":"Pedersen","year":"2002"},{"key":"10.1016\/j.ymeth.2026.04.003_b0060","doi-asserted-by":"crossref","unstructured":"R. Trias, B. M\u00e9nez, P. le Campion, Y. Zivanovic, L. Lecourt, A. Lecoeuvre, P. Schmitt-Kopplin, J. Uhl, S. Gislason, H. Alfred sson, K. Mesfin, S. \u00d3. Sn\u00e6bj\u00f6rnsd\u00f3ttir, E. S. Arad\u00f3ttir, I. Gunnarsson, J. M. Matter, M. Stute, E. H. Oelkers, E. G\u00e9rard, High reactivity of deep biota under anthropogenic CO2 injection into basalt, Nat. Comm. 8 (1) (2017) 1063. doi:10.1038\/s41467-017-01288-8.","DOI":"10.1038\/s41467-017-01288-8"},{"issue":"11","key":"10.1016\/j.ymeth.2026.04.003_b0065","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1093\/femsec\/fiae142","article-title":"Artificial subsurface lithoautotrophic microbial ecosystems and gas storage in deep subsurface","volume":"100","author":"Ranchou-Peyruse","year":"2024","journal-title":"FEMS Microbiol. Ecol."},{"issue":"4","key":"10.1016\/j.ymeth.2026.04.003_b0070","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1128\/MMBR.67.4.503-549.2003","article-title":"Recent advances in petroleum microbiology","volume":"67","author":"Hamme","year":"2003","journal-title":"Microbiol. Molec. Biol. Revs."},{"issue":"10","key":"10.1016\/j.ymeth.2026.04.003_b0075","doi-asserted-by":"crossref","first-page":"3953","DOI":"10.1111\/1462-2920.14745","article-title":"Geological gas-storage shapes deep life","volume":"21","author":"Ranchou-Peyruse","year":"2019","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.ymeth.2026.04.003_b0080","doi-asserted-by":"crossref","first-page":"3400","DOI":"10.1039\/D2EE00765G","article-title":"Geological storage of hydrogen in deep aquifers an experimental multidisciplinary study","volume":"15","author":"Haddad","year":"2022","journal-title":"Energy Environ. Sci."},{"key":"10.1016\/j.ymeth.2026.04.003_b0085","doi-asserted-by":"crossref","DOI":"10.1016\/j.eti.2023.103375","article-title":"Indirect in situ bioleaching is an emerging tool for accessing deeply buried metal reserves, but can the process be managed? a case study of copper leaching at 1 km depth","volume":"32","author":"Bomberg","year":"2023","journal-title":"Environ. Tech. & Innovation"},{"key":"10.1016\/j.ymeth.2026.04.003_b0090","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.bpc.2013.06.019","article-title":"Pressure as an environmental parameter for microbial life - a review","volume":"183","author":"Picard","year":"2013","journal-title":"Biophy. Chem."},{"key":"10.1016\/j.ymeth.2026.04.003_b0095","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2022.867340","article-title":"Characterizing the piezosphere: the effects of decompression on microbial growth dynamics","volume":"13","author":"Cario","year":"2022","journal-title":"Front. Microbiol."},{"issue":"12","key":"10.1016\/j.ymeth.2026.04.003_b0100","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1093\/femsec\/fiae151","article-title":"Microfluidics for studying the deep underground biosphere: from applications to fundamentals","volume":"100","author":"Morais","year":"2024","journal-title":"FEMS Microbiol. Ecol."},{"issue":"12","key":"10.1016\/j.ymeth.2026.04.003_b0105","doi-asserted-by":"crossref","first-page":"3140","DOI":"10.1111\/j.1462-2920.2009.02018.x","article-title":"Culturable prokaryotic diversity of deep, gas hydrate sediments: first use of a continuous high-pressure, anaerobic, enrichment and isolation system for subseafloor sediments (DeepIsoBUG)","volume":"11","author":"Parkes","year":"2009","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.ymeth.2026.04.003_b0110","doi-asserted-by":"crossref","first-page":"453","DOI":"10.3389\/fmicb.2019.00453","article-title":"Pressure-retaining sampler and high-pressure systems to study deep-sea microbes under in situ conditions","volume":"10","author":"Garel","year":"2019","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ymeth.2026.04.003_b0115","doi-asserted-by":"crossref","unstructured":"S. Dupraz, M. Parmentier, B. M\u00e9nez F. Guyot, Experimental and numerical modeling of bacterially induced pH increase and calcite precipitation in saline aquifers, Chem.l Geol. 265 (1-2) (2009) 44-53. doi:10.1016\/j.chemgeo.2009.05.003.","DOI":"10.1016\/j.chemgeo.2009.05.003"},{"key":"10.1016\/j.ymeth.2026.04.003_b0120","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1038\/nrm4041","article-title":"Microfluidics: reframing biological enquiry","volume":"16","author":"Duncombe","year":"2015","journal-title":"Nat. Rev. Mol. Cell Biol."},{"issue":"7101","key":"10.1016\/j.ymeth.2026.04.003_b0125","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1038\/nature05063","article-title":"Cells on chips","volume":"442","author":"El-Ali","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.ymeth.2026.04.003_b0130","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2022.866681","article-title":"High-pressure microfluidics for ultra-fast microbial phenotyping","volume":"13","author":"Cario","year":"2022","journal-title":"Front. Microbiol."},{"issue":"39","key":"10.1016\/j.ymeth.2026.04.003_b0135","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adi1884","article-title":"Alkaline vents recreated in two dimensions to study pH gradients, precipitation morphology, and molecule accumulation","volume":"9","author":"Weingart","year":"2023","journal-title":"Sci. Adv."},{"key":"10.1016\/j.ymeth.2026.04.003_b0140","unstructured":"S. Marre, C. Lecoutre, Y. Garrabos, C. Fauveau, A. Cario, O. Nguyen. (2021), Sapphire microreactors, (France)."},{"key":"10.1016\/j.ymeth.2026.04.003_b0145","doi-asserted-by":"crossref","first-page":"4382","DOI":"10.1039\/C4LC00608A","article-title":"Chip-off-the-old-rock: the study of reservoir-relevant geological processes with real-rock micromodels","volume":"14","author":"Song","year":"2014","journal-title":"Lab Chip"},{"key":"10.1016\/j.ymeth.2026.04.003_b0150","doi-asserted-by":"crossref","DOI":"10.1029\/2011WR011192","article-title":"Pore\u2010scale simulation of mixing\u2010induced calcium carbonate precipitation and dissolution in a microfluidic pore network","volume":"48","author":"Yoon","year":"2012","journal-title":"Water Resour. Res."},{"issue":"1","key":"10.1016\/j.ymeth.2026.04.003_b0155","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1002\/(SICI)1097-0290(19960705)51:1<120::AID-BIT14>3.0.CO;2-U","article-title":"A method for measuring bacterial chemotaxis parameters in a microcapillary","volume":"51","author":"Liu","year":"1996","journal-title":"Biotech. Bioeng."},{"issue":"3","key":"10.1016\/j.ymeth.2026.04.003_b0160","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.resmic.2014.01.007","article-title":"Filamentous Escherichia coli cells swimming in tapered microcapillaries","volume":"165","author":"Jaimes-Lizcano","year":"2014","journal-title":"Res. Microbiol."},{"key":"10.1016\/j.ymeth.2026.04.003_b0165","series-title":"Theory of Elasticity","author":"Landau","year":"1986"},{"issue":"3","key":"10.1016\/j.ymeth.2026.04.003_b0170","doi-asserted-by":"crossref","first-page":"221","DOI":"10.18280\/i2m.190307","article-title":"Small but Powerful Optically: Glass Microcapillaries for Studying complex Fluids or Biological Systems with Sub-microlitre Samples under Harsh Conditions","volume":"19","author":"Zhou","year":"2020","journal-title":"Instrumentation, Mesure, M\u00e9trologie"},{"key":"10.1016\/j.ymeth.2026.04.003_b0175","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.fluid.2014.08.032","article-title":"Quantitative Raman spectroscopic investigation of geo-fluids high-pressure phase equilibria: Part I. Accurate calibration and determination of CO2 solubility in water from 273.15 to 573.15 K and from 10 to 120 MPa","volume":"382","author":"Guo","year":"2014","journal-title":"Fluid Phase Equilib."},{"key":"10.1016\/j.ymeth.2026.04.003_b0180","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1126\/science.1086823","article-title":"Extending the upper temperature limit for life. science 301: 934","author":"Kashe","year":"2003","journal-title":"Science 301"},{"issue":"31","key":"10.1016\/j.ymeth.2026.04.003_b0185","doi-asserted-by":"crossref","first-page":"10949","DOI":"10.1073\/pnas.0712334105","article-title":"Cell proliferation at 122 \u25e6C and isotopically heavy CH4 production by a hyperthermophilic methanogen under high-pressure cultivation","volume":"105","author":"Takai","year":"2008","journal-title":"Proc. Nat. Acad. Sci."},{"issue":"9","key":"10.1016\/j.ymeth.2026.04.003_b0190","doi-asserted-by":"crossref","first-page":"3775","DOI":"10.1002\/2016GC006449","article-title":"Brucite chimney formation and carbonate alteration at the shinkai seep eld, a serpentinite-hosted vent system in the southern mariana forearc","volume":"17","author":"Okumura","year":"2016","journal-title":"Geochem. Geophys. Geosyst."},{"issue":"7","key":"10.1016\/j.ymeth.2026.04.003_b0195","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.3390\/microorganisms11071629","article-title":"The mystery of piezophiles: Understudied microorganisms from the deep, dark subsurface","volume":"11","author":"Scheffer","year":"2023","journal-title":"Microorganisms"},{"key":"10.1016\/j.ymeth.2026.04.003_b0200","doi-asserted-by":"crossref","unstructured":"N. Macedo Portela da Silva, J. Letourneau, F. Espitalier, L. Prat, Transparent and inexpensive microfluidic device for two-phase flow systems with high-pressure performance, Chem. Eng. Technol. 37 (11) (2014) 1929-1937. doi:10.1002\/ceat.201400028.","DOI":"10.1002\/ceat.201400028"},{"issue":"38","key":"10.1016\/j.ymeth.2026.04.003_b0205","doi-asserted-by":"crossref","first-page":"12569","DOI":"10.1021\/acs.langmuir.9b01146","article-title":"Gas hydrate crystallization in thin glass capillaries: Roles of supercooling and wettability","volume":"35","author":"Touil","year":"2019","journal-title":"Langmuir"},{"key":"10.1016\/j.ymeth.2026.04.003_b0210","doi-asserted-by":"crossref","DOI":"10.1016\/j.ces.2020.115960","article-title":"Determination of mass transfer coefficients in high- pressure two-phase flows in capillaries using Raman spectroscopy","volume":"228","author":"Deleau","year":"2020","journal-title":"Chem. Eng. Sci."},{"key":"10.1016\/j.ymeth.2026.04.003_b0215","doi-asserted-by":"crossref","first-page":"3314","DOI":"10.1039\/C5LC00544B","article-title":"Functionalization of micromodels with kaolinite for investigation of low salinity oil-recovery processes","volume":"15","author":"Song","year":"2015","journal-title":"Lab Chip"},{"key":"10.1016\/j.ymeth.2026.04.003_b0220","unstructured":"D. W. Cromey, The importance of #1.5 thickness coverslips for microscopy, https:\/\/microscopy.arizona.edu\/sites\/default\/les\/2023- 03\/coverslips_for_microscopy.pdf, accessed: 2025-09-03."},{"key":"10.1016\/j.ymeth.2026.04.003_b0225","doi-asserted-by":"crossref","unstructured":"K. Alain, A. Postec, E. Grinsard, F. Lesongeur, D. Prieur, A. Godfroy, Thermodesulfatator atlanticus sp. nov., a thermophilic, chemolithoautotrophic, sulfate-reducing bacterium isolated from a mid-atlantic ridge hydrothermal vent, Int. J. Syst. Evol. Microbiol. 60 (Pt 1) (2010) 33-38. doi:10.1099\/ijs.0.009449-0.","DOI":"10.1099\/ijs.0.009449-0"},{"key":"10.1016\/j.ymeth.2026.04.003_b0230","doi-asserted-by":"crossref","unstructured":"A. Bellack, H. Huber, R. Rachel, G. Wanner, R. Wirth, Methanocaldococcus villosus sp. nov., a heavily flagellated archaeon that adheres to surfaces and forms cell-cell contacts, Int. J. Syst. Evol. Microbiol. 61 (Pt 6) (2011) 1239-1245. doi: 10.1099\/ijs.0.023663-0.","DOI":"10.1099\/ijs.0.023663-0"},{"key":"10.1016\/j.ymeth.2026.04.003_b0235","doi-asserted-by":"crossref","first-page":"492","DOI":"10.3389\/fmicb.2015.00492","article-title":"Methanogenic archaea and sulfate reducing bacteria co-cultured on acetate: teamwork or coexistence?","volume":"6","author":"Ozuolmez","year":"2015","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ymeth.2026.04.003_b0240","doi-asserted-by":"crossref","first-page":"766","DOI":"10.3389\/fmicb.2017.00766","article-title":"Co-existence of methanogenesis and sulfate reduction with common substrates in sulfate-rich estuarine sediments","volume":"8","author":"Sela-Adler","year":"2017","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ymeth.2026.04.003_b0245","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1038\/239500a0","article-title":"Chemotaxis in Escherichia coli analysed by three-dimensional tracking","volume":"239","author":"Berg","year":"1972","journal-title":"Nature"},{"issue":"15","key":"10.1016\/j.ymeth.2026.04.003_b0250","doi-asserted-by":"crossref","first-page":"3060","DOI":"10.1016\/j.bpj.2023.06.008","article-title":"Two-state swimming: strategy and survival of a model bacterial predator in response to environmental cues","volume":"122","author":"Xu","year":"2023","journal-title":"Biophys. J ."},{"key":"10.1016\/j.ymeth.2026.04.003_b0255","doi-asserted-by":"crossref","DOI":"10.1103\/PhysRevLett.106.018101","article-title":"Differential dynamic microscopy of bacterial motility","volume":"106","author":"Wilson","year":"2011","journal-title":"Phys. Rev. Lett."},{"key":"10.1016\/j.ymeth.2026.04.003_b0260","article-title":"Holographic imaging reveals the mechanism of wall entrapment in swimming bacteria","volume":"7","author":"Bianchi","year":"2017","journal-title":"Phys. Rev. X"},{"key":"10.1016\/j.ymeth.2026.04.003_b0265","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"issue":"16","key":"10.1016\/j.ymeth.2026.04.003_b0270","doi-asserted-by":"crossref","first-page":"2389","DOI":"10.1093\/bioinformatics\/btu202","article-title":"Thunder-STORM: a comprehensive ImageJ plug-in for PALM and STORM data analysis and super-resolution imaging","volume":"30","author":"Ovesn\u00fd","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ymeth.2026.04.003_b0275","doi-asserted-by":"crossref","unstructured":"H. Shen, L. J. Tauzin, R. Baiyasi, W. Wang, N. Moringo, B. Shuang, C. F. Landes, Single particle tracking: From theory to biophysical applications, Chem. Rev. 117 (11) (2017) 7331-7376, pMID: 28520419. doi:10.1021\/acs.chemrev.6b00815.","DOI":"10.1021\/acs.chemrev.6b00815"},{"key":"10.1016\/j.ymeth.2026.04.003_b0280","doi-asserted-by":"crossref","first-page":"29018","DOI":"10.1039\/C8CP04043E","article-title":"Bayesian analysis of single-particle tracking data using the nested-sampling algorithm: maximum-likelihood model selection applied to stochastic-diffusivity data","volume":"20","author":"Thapa","year":"2018","journal-title":"Phys. Chem. Chem. Phys."},{"issue":"6","key":"10.1016\/j.ymeth.2026.04.003_b0285","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1063\/1.1138619","article-title":"Position measurement with a resolution and noise-limited instrument","volume":"57","author":"Bobro","year":"1986","journal-title":"Rev. Sci. Instrum."},{"key":"10.1016\/j.ymeth.2026.04.003_b0290","series-title":"Numerical Recipes, the Art of Scientific Computing (FORTRAN Version)","author":"Press","year":"1992"},{"key":"10.1016\/j.ymeth.2026.04.003_b0295","article-title":"Thickening, temperature and guest concentration of the meltwater layer growing over a slowly melting gas hydrate","author":"Samar","year":"2025","journal-title":"Chem. Eng. Sci."},{"issue":"9","key":"10.1016\/j.ymeth.2026.04.003_b0300","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1016\/j.gca.2011.02.010","article-title":"On the molecular diffusion coefficients of dissolved CO2, HCO\u2212, and CO2\u2212 and their dependence on isotopic mass","volume":"75","author":"Zeebe","year":"2011","journal-title":"Geochim. Cosmochim. Acta"},{"issue":"6","key":"10.1016\/j.ymeth.2026.04.003_b0305","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1128\/aem.32.6.781-791.1976","article-title":"New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere","volume":"32","author":"Balch","year":"1976","journal-title":"Appl. Environ. Microbiol."},{"issue":"4","key":"10.1016\/j.ymeth.2026.04.003_b0310","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/s00792-004-0386-3","article-title":"Geochemical and microbiological evidence for a hydrogen-based, hyperthermophilic subsurface lithoautotrophic microbial ecosystem (hyperslime) beneath an active deep-sea hydrothermal field","volume":"8","author":"Takai","year":"2004","journal-title":"Extremophiles"},{"issue":"6","key":"10.1016\/j.ymeth.2026.04.003_b0315","doi-asserted-by":"crossref","first-page":"1970","DOI":"10.1111\/1462-2920.13173","article-title":"Subsea floor microbial communities in hydrogen-rich vent fluids from hydrothermal systems along the mid-cayman rise","volume":"18","author":"Reveillaud","year":"2016","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.ymeth.2026.04.003_b0320","series-title":"Handbook of Chemistry and Physics","year":"1967"},{"key":"10.1016\/j.ymeth.2026.04.003_b0325","first-page":"363","volume":"Vol. 1","author":"Pfeffer","year":"1884","journal-title":"Locomotorische Richtungsbewegungen Durch Chemische Reize"},{"issue":"3","key":"10.1016\/j.ymeth.2026.04.003_b0330","doi-asserted-by":"crossref","first-page":"563","DOI":"10.3390\/microorganisms10030563","article-title":"Methods to evaluate bacterial motility and its role in bacterial host interactions","volume":"10","author":"Palma","year":"2022","journal-title":"Microorganisms"},{"key":"10.1016\/j.ymeth.2026.04.003_b0335","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1038\/s41579-021-00626-4","article-title":"Bacterial motility: machinery and mechanisms","volume":"20","author":"Wadhwa","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.ymeth.2026.04.003_b0340","doi-asserted-by":"crossref","first-page":"4020","DOI":"10.1128\/JB.181.13.4020-4025.1999","article-title":"Temperature-sensitive motility of Sulfolobus acidocaldarius influences population distribution in extreme environments","volume":"181","author":"Lewus","year":"1999","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.ymeth.2026.04.003_b0345","doi-asserted-by":"crossref","first-page":"3379","DOI":"10.1038\/s41467-020-16628-4","article-title":"Contactless probing of poly-crystalline methane hydrate at pore scale suggests weaker tensile properties than thought","volume":"11","author":"Atig","year":"2020","journal-title":"Nat. Comm."},{"issue":"5","key":"10.1016\/j.ymeth.2026.04.003_b0350","doi-asserted-by":"crossref","first-page":"401","DOI":"10.2323\/jgam.33.401","article-title":"Influence of Redox potential on Biomethanation of H2 and CO2 by Methanobacterium thermoautotrophicum in EH-Stat batch Cultures","volume":"33","author":"Jee","year":"1987","journal-title":"J. Gen. Appl. Microbiol."},{"issue":"3","key":"10.1016\/j.ymeth.2026.04.003_b0355","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S0012-821X(01)00257-6","article-title":"In situ measurement of dissolved H2 and H2S in high-temperature hydrothermal vent fluids at the Main Endeavour Field, Juan de Fuca Ridge","volume":"186","author":"Ding","year":"2001","journal-title":"Earth Planet. Sci. Lett."},{"key":"10.1016\/j.ymeth.2026.04.003_b0360","doi-asserted-by":"crossref","unstructured":"F. Lucile, P. C\u00e9zac, F. Contamine, J.-P Serin, D. Houssin, P. Arpentinier, Solubility of Carbon Dioxide in Water and Aqueous Solution Containing Sodium Hydroxide at Temperatures from (293.15 to 393.15) K and Pressure up to 5 MPa: Experimental Measurements, J. Chem. Eng. Data 57 (3) (2012) 784-789. doi:10.1021\/je200991x.","DOI":"10.1021\/je200991x"},{"key":"10.1016\/j.ymeth.2026.04.003_b0365","doi-asserted-by":"crossref","unstructured":"R. Wiebe, V. L. Gaddy, The solubility of carbon dioxide in water at various temperatures from 12 to 40 \u25e6 and at pressures to 500 atmospheres. critical phenomena, J. Am. Chem. Soc. 62 (4) (1940) 815-817. doi:10.1021\/ja01861a033.","DOI":"10.1021\/ja01861a033"},{"issue":"1","key":"10.1016\/j.ymeth.2026.04.003_b0370","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1021\/ja01316a022","article-title":"The solubility of hydrogen in water at 0, 50, 75 and 100 \u25e6 from 25 to 1000 atmospheres","volume":"56","author":"Wiebe","year":"1934","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.ymeth.2026.04.003_b0375","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.1016\/j.bpj.2012.03.033","article-title":"Microscopic analysis of bacterial motility at high pressure","volume":"102","author":"Nishiyama","year":"2012","journal-title":"Biophysical J."},{"key":"10.1016\/j.ymeth.2026.04.003_b0380","doi-asserted-by":"crossref","unstructured":"S. Wang, T. Zhou, Z. Pan, J. P. M. Trusler, Diffusion Coefficients of N2O and H2 in Water at Temperatures between 298.15 and 423.15 K with Pressures up to 30 MPa, J. Chem. Eng. Data 68 (6) (2023) 1313-1319. doi:10.1021\/acs.jced.3c00085.","DOI":"10.1021\/acs.jced.3c00085"}],"container-title":["Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202326000848?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202326000848?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T21:52:18Z","timestamp":1778795538000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046202326000848"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":76,"alternative-id":["S1046202326000848"],"URL":"https:\/\/doi.org\/10.1016\/j.ymeth.2026.04.003","relation":{},"ISSN":["1046-2023"],"issn-type":[{"value":"1046-2023","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Under the microscope: microbial life at pore scale under extreme deep-environment conditions","name":"articletitle","label":"Article Title"},{"value":"Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymeth.2026.04.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}