{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:25:19Z","timestamp":1787023519641,"version":"build-2736575974"},"reference-count":67,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJSP2124"],"award-info":[{"award-number":["JPMJSP2124"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24H02060"],"award-info":[{"award-number":["JP24H02060"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP23K05778"],"award-info":[{"award-number":["JP23K05778"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP23KJ0283"],"award-info":[{"award-number":["JP23KJ0283"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24H02030"],"award-info":[{"award-number":["JP24H02030"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP23K26997"],"award-info":[{"award-number":["JP23K26997"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP24zf0127011"],"award-info":[{"award-number":["JP24zf0127011"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Methods"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.ymeth.2026.06.007","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T06:39:19Z","timestamp":1782715159000},"page":"59-69","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples"],"prefix":"10.1016","volume":"254","author":[{"given":"Yasuhiro","family":"Kozono","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyohei","family":"Mikami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunta","family":"Yorimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taisei","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hibiki","family":"Okamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyin","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Hoshino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takumi","family":"Kamiyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuya","family":"Sanaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miho","family":"Asaoka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonoko","family":"Ohsawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shota","family":"Azuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuka W.","family":"Iwasaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuji","family":"Shigenobu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryusuke","family":"Niwa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymeth.2026.06.007_b0005","doi-asserted-by":"crossref","first-page":"3632","DOI":"10.1242\/dev.140137","article-title":"Mapping a multiplexed zoo of mRNA expression","volume":"143","author":"Choi","year":"2016","journal-title":"Development"},{"key":"10.1016\/j.ymeth.2026.06.007_b0010","article-title":"Third-generation in situ hybridization chain reaction: multiplexed, quantitative, sensitive, versatile, robust","author":"Choi","year":"2018","journal-title":"Development 145"},{"key":"10.1016\/j.ymeth.2026.06.007_b0015","doi-asserted-by":"crossref","DOI":"10.1083\/jcb.202303125","article-title":"Tejas functions as a core component in nuage assembly and precursor processing in Drosophila piRNA biogenesis","volume":"222","author":"Lin","year":"2023","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.ymeth.2026.06.007_b0020","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1101\/gad.330464.119","article-title":"A single-cell atlas of the developing Drosophila ovary identifies follicle stem cell progenitors","volume":"34","author":"Slaidina","year":"2020","journal-title":"Genes Dev."},{"key":"10.1016\/j.ymeth.2026.06.007_b0025","doi-asserted-by":"crossref","first-page":"75","DOI":"10.3389\/fnmol.2020.00075","article-title":"Modified in situ Hybridization Chain Reaction using Short Hairpin DNAs","volume":"13","author":"Tsuneoka","year":"2020","journal-title":"Front. Mol. Neurosci."},{"key":"10.1016\/j.ymeth.2026.06.007_b0030","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.jmoldx.2011.08.002","article-title":"RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues","volume":"14","author":"Wang","year":"2012","journal-title":"J. Mol. Diagn."},{"key":"10.1016\/j.ymeth.2026.06.007_b0035","doi-asserted-by":"crossref","first-page":"e165","DOI":"10.1093\/nar\/gkw784","article-title":"smiFISH and FISH-quant - a flexible single RNA detection approach with super-resolution capability","volume":"44","author":"Tsanov","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ymeth.2026.06.007_b0040","doi-asserted-by":"crossref","DOI":"10.1080\/19336934.2024.2428499","article-title":"A fast in situ hybridization chain reaction method in Drosophila embryos and ovaries","volume":"19","author":"Mikami","year":"2025","journal-title":"Fly"},{"key":"10.1016\/j.ymeth.2026.06.007_b0045","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0040675","article-title":"Fast and non-toxic in situ hybridization without blocking of repetitive sequences","volume":"7","author":"Matthiesen","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.ymeth.2026.06.007_b0050","doi-asserted-by":"crossref","DOI":"10.1242\/dev.205225","article-title":"Xrp1 drives damage-induced cellular plasticity of enteroendocrine cells in the adult Drosophila midgut","author":"Qian","year":"2026","journal-title":"Development 153"},{"key":"10.1016\/j.ymeth.2026.06.007_b0055","doi-asserted-by":"crossref","first-page":"1808","DOI":"10.1038\/nprot.2012.105","article-title":"Dual fluorescence detection of protein and RNA in Drosophila tissues","volume":"7","author":"Toledano","year":"2012","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ymeth.2026.06.007_b0060","doi-asserted-by":"crossref","first-page":"2983","DOI":"10.15252\/embj.201489050","article-title":"Regulation of Drosophila intestinal stem cell maintenance and differentiation by the transcription factor Escargot","volume":"33","author":"Loza-Coll","year":"2014","journal-title":"EMBO J."},{"key":"10.1016\/j.ymeth.2026.06.007_b0065","first-page":"1","article-title":"Protein-responsive gut hormone tachykinin directs food choice and impacts lifespan","author":"Ahrentl\u00f8v","year":"2025","journal-title":"Nat. Metab."},{"key":"10.1016\/j.ymeth.2026.06.007_b0070","doi-asserted-by":"crossref","first-page":"6525","DOI":"10.1038\/s41467-022-34270-0","article-title":"A hierarchical transcription factor cascade regulates enteroendocrine cell diversity and plasticity in Drosophila","volume":"13","author":"Guo","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ymeth.2026.06.007_b0075","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1016\/j.peptides.2003.10.023","article-title":"Functions and analysis of the seminal fluid proteins of male Drosophila melanogaster fruit flies","volume":"25","author":"Chapman","year":"2004","journal-title":"Peptides"},{"key":"10.1016\/j.ymeth.2026.06.007_b0080","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/0092-8674(88)90192-4","article-title":"A male accessory gland peptide that regulates reproductive behavior of female D. melanogaster","volume":"54","author":"Chen","year":"1988","journal-title":"Cell"},{"key":"10.1016\/j.ymeth.2026.06.007_b0085","doi-asserted-by":"crossref","first-page":"9929","DOI":"10.1073\/pnas.1631700100","article-title":"Sex-peptide is the molecular basis of the sperm effect in Drosophila melanogaster","volume":"100","author":"Liu","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ymeth.2026.06.007_b0090","doi-asserted-by":"crossref","first-page":"1731","DOI":"10.1080\/00222930701504797","article-title":"Geographical distributions and host associations of larval parasitoids of frugivorous Drosophilidae in Japan","volume":"41","author":"Mitsui","year":"2007","journal-title":"J. Nat. Hist."},{"key":"10.1016\/j.ymeth.2026.06.007_b0095","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adq8771","article-title":"Parasitoid wasp venoms degrade Drosophila imaginal discs for successful parasitism","volume":"11","author":"Kamiyama","year":"2025","journal-title":"Sci. Adv."},{"key":"10.1016\/j.ymeth.2026.06.007_b0100","doi-asserted-by":"crossref","first-page":"E21","DOI":"10.1371\/journal.pbio.0000021","article-title":"Developmental origin and evolution of bacteriocytes in the aphid-Buchnera symbiosis","volume":"1","author":"Braendle","year":"2003","journal-title":"PLoS Biol."},{"key":"10.1016\/j.ymeth.2026.06.007_b0105","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1002\/jez.b.3","article-title":"A comparison of parthenogenetic and sexual embryogenesis of the pea aphid Acyrthosiphon pisum (Hemiptera: Aphidoidea)","volume":"295","author":"Miura","year":"2003","journal-title":"J. Exp. Zool. B Mol. Dev. Evol."},{"key":"10.1016\/j.ymeth.2026.06.007_b0110","article-title":"Aphids evolved novel secreted proteins for symbiosis with bacterial endosymbiont","volume":"280","author":"Shigenobu","year":"2013","journal-title":"Proc. Biol. Sci."},{"key":"10.1016\/j.ymeth.2026.06.007_b0115","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1264\/jsme2.ME18148","article-title":"Antimicrobial activities of cysteine-rich peptides specific to bacteriocytes of the pea aphid Acyrthosiphon pisum","volume":"34","author":"Uchi","year":"2019","journal-title":"Microbes Environ."},{"key":"10.1016\/j.ymeth.2026.06.007_b0120","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/s00427-007-0142-7","article-title":"Apvasa marks germ-cell migration in the parthenogenetic pea aphid Acyrthosiphon pisum (Hemiptera: Aphidoidea)","volume":"217","author":"Chang","year":"2007","journal-title":"Dev. Genes Evol."},{"key":"10.1016\/j.ymeth.2026.06.007_b0125","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1002\/(SICI)1098-2795(200004)55:4<364::AID-MRD2>3.0.CO;2-8","article-title":"Cloning and characterization of a vasa-like gene in rainbow trout and its expression in the germ cell lineage","volume":"55","author":"Yoshizaki","year":"2000","journal-title":"Mol. Reprod. Dev"},{"key":"10.1016\/j.ymeth.2026.06.007_b0130","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.ydbio.2006.10.001","article-title":"A novel transforming growth factor-beta superfamily member expressed in gonadal somatic cells enhances primordial germ cell and spermatogonial proliferation in rainbow trout (Oncorhynchus mykiss)","volume":"301","author":"Sawatari","year":"2007","journal-title":"Dev. Biol."},{"key":"10.1016\/j.ymeth.2026.06.007_b0135","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1007\/s10695-023-01212-6","article-title":"Subcellular localization of Na+\/K+-ATPase isoforms resolved by in situ hybridization chain reaction in the gill of chum salmon at freshwater and seawater","volume":"49","author":"Wong","year":"2023","journal-title":"Fish Physiol. Biochem."},{"key":"10.1016\/j.ymeth.2026.06.007_b0140","unstructured":"Allen Reference Atlas \u2013 Mouse Brain [brain atlas]. Available from mouse.brain-map.org, (n.d.)."},{"key":"10.1016\/j.ymeth.2026.06.007_b0145","doi-asserted-by":"crossref","first-page":"a002774","DOI":"10.1101\/cshperspect.a002774","article-title":"RNA granules in germ cells","volume":"3","author":"Voronina","year":"2011","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"10.1016\/j.ymeth.2026.06.007_b0150","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.semcdb.2023.06.010","article-title":"P-body-like condensates in the germline","volume":"157","author":"Cassani","year":"2024","journal-title":"Semin. Cell Dev. Biol."},{"key":"10.1016\/j.ymeth.2026.06.007_b0155","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3002069","article-title":"Plasticity of Drosophila germ granules during germ cell development","volume":"21","author":"Hakes","year":"2023","journal-title":"PLoS Biol."},{"key":"10.1016\/j.ymeth.2026.06.007_b0160","doi-asserted-by":"crossref","DOI":"10.1093\/genetics\/iyae217","article-title":"Origin and establishment of the germline in Drosophila melanogaster","author":"Chen","year":"2025","journal-title":"Genetics 229"},{"key":"10.1016\/j.ymeth.2026.06.007_b0165","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.1261\/rna.078576.120","article-title":"The kinesin KIF1C transports APC-dependent mRNAs to cell protrusions","volume":"27","author":"Pichon","year":"2021","journal-title":"RNA"},{"key":"10.1016\/j.ymeth.2026.06.007_b0170","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1038\/s41596-022-00750-2","article-title":"HT-smFISH: a cost-effective and flexible workflow for high-throughput single-molecule RNA imaging","volume":"18","author":"Safieddine","year":"2023","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ymeth.2026.06.007_b0175","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2020.100950","article-title":"Repression of G1\/S transition by transient inhibition of miR-10404 expression in Drosophila primordial germ cells","volume":"23","author":"Morita","year":"2020","journal-title":"iScience"},{"key":"10.1016\/j.ymeth.2026.06.007_b0180","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/0003-2697(84)90808-X","article-title":"Hybridization of nucleic acids immobilized on solid supports","volume":"138","author":"Meinkoth","year":"1984","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ymeth.2026.06.007_b0185","doi-asserted-by":"crossref","DOI":"10.1002\/cne.25622","article-title":"Comprehensive mapping of histamine H1 receptor mRNA in the mouse brain","volume":"532","author":"Futagawa","year":"2024","journal-title":"J Comp Neurol"},{"key":"10.1016\/j.ymeth.2026.06.007_b0190","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.92380.3","article-title":"Whole-brain in situ mapping of neuronal activation in Drosophila during social behaviors and optogenetic stimulation","volume":"12","author":"Watanabe","year":"2024","journal-title":"eLife"},{"key":"10.1016\/j.ymeth.2026.06.007_b0195","doi-asserted-by":"crossref","first-page":"3660","DOI":"10.1016\/j.cub.2023.07.043","article-title":"Re-examining the role of the dorsal fan-shaped body in promoting sleep in Drosophila","volume":"33","author":"De","year":"2023","journal-title":"Curr. Biol."},{"key":"10.1016\/j.ymeth.2026.06.007_b0200","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.devcel.2023.02.013","article-title":"A single-cell census of mouse limb development identifies complex spatiotemporal dynamics of skeleton formation","volume":"58","author":"Markman","year":"2023","journal-title":"Dev. Cell"},{"key":"10.1016\/j.ymeth.2026.06.007_b0205","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/j.cell.2018.06.021","article-title":"Molecular architecture of the mouse nervous system","volume":"174","author":"Zeisel","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.ymeth.2026.06.007_b0210","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1007\/s00429-020-02194-4","article-title":"Optimization and evaluation of fluorescence in situ hybridization chain reaction in cleared fresh-frozen brain tissues","volume":"226","author":"Kumar","year":"2021","journal-title":"Brain Struct. Funct."},{"key":"10.1016\/j.ymeth.2026.06.007_b0215","article-title":"Escalation of genome defense capacity enables control of an expanding meiotic driver","volume":"122","author":"Chen","year":"2025","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ymeth.2026.06.007_b0220","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adf3525","article-title":"A CRE\/DRE dual recombinase transgenic mouse reveals synaptic zinc-mediated thalamocortical neuromodulation","volume":"9","author":"Kouvaros","year":"2023","journal-title":"Sci. Adv."},{"key":"10.1016\/j.ymeth.2026.06.007_b0225","doi-asserted-by":"crossref","first-page":"2984","DOI":"10.1016\/j.cub.2021.04.052","article-title":"RNA degradation is required for the germ-cell to maternal transition in Drosophila","volume":"31","author":"Blatt","year":"2021","journal-title":"Curr. Biol."},{"key":"10.1016\/j.ymeth.2026.06.007_b0230","doi-asserted-by":"crossref","first-page":"7962","DOI":"10.1038\/ncomms8962","article-title":"Drosophila germ granules are structured and contain homotypic mRNA clusters","volume":"6","author":"Trcek","year":"2015","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ymeth.2026.06.007_b0235","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1038\/ncb3143","article-title":"Independent and coordinate trafficking of single Drosophila germ plasm mRNAs","volume":"17","author":"Little","year":"2015","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.ymeth.2026.06.007_b0240","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.molcel.2017.09.003","article-title":"P-body purification reveals the condensation of repressed mRNA regulons","volume":"68","author":"Hubstenberger","year":"2017","journal-title":"Mol. Cell"},{"key":"10.1016\/j.ymeth.2026.06.007_b0245","doi-asserted-by":"crossref","first-page":"4191","DOI":"10.1016\/j.molcel.2024.09.011","article-title":"Cell-cycle-dependent mRNA localization in P-bodies","volume":"84","author":"Safieddine","year":"2024","journal-title":"Mol. Cell"},{"key":"10.1016\/j.ymeth.2026.06.007_b0250","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1126\/science.280.5363.585","article-title":"Visualization of Single RNA Transcripts in Situ","volume":"280","author":"Femino","year":"1998","journal-title":"Science"},{"key":"10.1016\/j.ymeth.2026.06.007_b0255","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1038\/nmeth.1253","article-title":"Imaging individual mRNA molecules using multiple singly labeled probes","volume":"5","author":"Raj","year":"2008","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ymeth.2026.06.007_b0260","doi-asserted-by":"crossref","first-page":"15275","DOI":"10.1073\/pnas.0407024101","article-title":"Triggered amplification by hybridization chain reaction","volume":"101","author":"Dirks","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ymeth.2026.06.007_b0265","doi-asserted-by":"crossref","first-page":"6818","DOI":"10.1038\/s41598-017-07210-y","article-title":"DNA self-assembly catalyzed by artificial agents","volume":"7","author":"Shi","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ymeth.2026.06.007_b0270","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/ens.12281","article-title":"Application of next-generation sequencing to the study of non-model insects: Next-generation sequencing in entomology","volume":"21","author":"Wachi","year":"2018","journal-title":"Entomol. Sci."},{"key":"10.1016\/j.ymeth.2026.06.007_b0275","doi-asserted-by":"crossref","DOI":"10.3389\/fmars.2022.757921","article-title":"The next generation is here: a review of transcriptomic approaches in marine ecology","volume":"9","author":"Page","year":"2022","journal-title":"Front. Mar. Sci."},{"key":"10.1016\/j.ymeth.2026.06.007_b0280","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2109019118","article-title":"Toward a genome sequence for every animal: where are we now?","volume":"118","author":"Hotaling","year":"2021","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ymeth.2026.06.007_b0285","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1038\/nprot.2017.030","article-title":"mRNA quantification using single-molecule FISH in Drosophila embryos","volume":"12","author":"Trcek","year":"2017","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ymeth.2026.06.007_b0290","article-title":"Multidimensional quantitative analysis of mRNA expression within intact vertebrate embryos","author":"Trivedi","year":"2018","journal-title":"Development 145"},{"key":"10.1016\/j.ymeth.2026.06.007_b0295","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/s00441-022-03596-y","article-title":"Gene duplication of C-type natriuretic peptide-4 (CNP4) in teleost lineage elicits subfunctionalization of ancestral CNP","volume":"388","author":"Katayama","year":"2022","journal-title":"Cell Tissue Res."},{"key":"10.1016\/j.ymeth.2026.06.007_b0300","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1111\/dgd.12607","article-title":"Rapid and efficient generation of GFP-knocked-in Drosophila by the CRISPR-Cas9-mediated genome editing","volume":"61","author":"Kina","year":"2019","journal-title":"Dev. Growth Differ."},{"key":"10.1016\/j.ymeth.2026.06.007_b0305","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1010709","article-title":"Recognition of commensal bacterial peptidoglycans defines Drosophila gut homeostasis and lifespan","volume":"19","author":"Onuma","year":"2023","journal-title":"PLoS Genet."},{"key":"10.1016\/j.ymeth.2026.06.007_b0310","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1095\/biolreprod.107.064667","article-title":"Flow-cytometric isolation of testicular germ cells from rainbow trout (Oncorhynchus mykiss) carrying the green fluorescent protein gene driven by trout vasa regulatory regions","volume":"78","author":"Yano","year":"2008","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.ymeth.2026.06.007_b0315","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1387\/ijdb.10853829","article-title":"Germ cell-specific expression of green fluorescent protein in transgenic rainbow trout under control of the rainbow trout vasa-like gene promoter","volume":"44","author":"Yoshizaki","year":"2000","journal-title":"Int. J. Dev. Biol."},{"key":"10.1016\/j.ymeth.2026.06.007_b0320","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1111\/j.1365-3032.2009.00676.x","article-title":"Effects of Asobara japonica venom on larval survival of host and nonhost Drosophila species","volume":"34","author":"Furihata","year":"2009","journal-title":"Physiol. Entomol."},{"key":"10.1016\/j.ymeth.2026.06.007_b0325","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1242\/dev.120.4.947","article-title":"The Drosophila fusome, a germline-specific organelle, contains membrane skeletal proteins and functions in cyst formation","volume":"120","author":"Lin","year":"1994","journal-title":"Development"},{"key":"10.1016\/j.ymeth.2026.06.007_b0330","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ymeth.2026.06.007_b0335","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.37949","article-title":"Phase transitioned nuclear Oskar promotes cell division of Drosophila primordial germ cells","volume":"7","author":"Kistler","year":"2018","journal-title":"eLife"}],"container-title":["Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202326001544?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202326001544?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:47:29Z","timestamp":1787021249000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046202326001544"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":67,"alternative-id":["S1046202326001544"],"URL":"https:\/\/doi.org\/10.1016\/j.ymeth.2026.06.007","relation":{},"ISSN":["1046-2023"],"issn-type":[{"value":"1046-2023","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples","name":"articletitle","label":"Article Title"},{"value":"Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymeth.2026.06.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}