{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T07:48:10Z","timestamp":1792136890418,"version":"4.4.0"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82072332"],"award-info":[{"award-number":["82072332"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013076","name":"National Major Science and Technology Projects of China","doi-asserted-by":"publisher","award":["2025ZD01900100"],"award-info":[{"award-number":["2025ZD01900100"]}],"id":[{"id":"10.13039\/501100013076","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018536","name":"National Mega Project on Major Infectious Disease Prevention","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018536","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015800","name":"Jilin Province Development and Reform Commission","doi-asserted-by":"publisher","award":["2024C015-4"],"award-info":[{"award-number":["2024C015-4"]}],"id":[{"id":"10.13039\/100015800","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Methods"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.ymeth.2026.10.003","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T23:24:50Z","timestamp":1791933890000},"page":"170-180","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Site-specific integration of canine distemper virus hemagglutinin protein in Expi293F cell lines contributes to protective neutralizing antibody responses in mice and dogs"],"prefix":"10.1016","volume":"256","author":[{"given":"Yu","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingxin","family":"Bi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxi","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuren","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohan","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianghong","family":"Xue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymeth.2026.10.003_b0225","doi-asserted-by":"crossref","first-page":"12579","DOI":"10.1128\/JVI.77.23.12579-12591.2003","article-title":"A ferret model of canine distemper virus virulence and immunosuppression","volume":"77","author":"von Messling","year":"2003","journal-title":"J. Virol."},{"key":"10.1016\/j.ymeth.2026.10.003_b0045","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.vetmic.2014.05.019","article-title":"Phylogenetic evidence of a new canine distemper virus lineage among domestic dogs in Colombia, South America","volume":"172","author":"Espinal","year":"2014","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.ymeth.2026.10.003_b0140","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1186\/s12917-016-0702-z","article-title":"Diversity of susceptible hosts in canine distemper virus infection: a systematic review and data synthesis","volume":"12","author":"Martinez-Gutierrez","year":"2016","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.ymeth.2026.10.003_b0090","first-page":"11","article-title":"Canine and phocine distemper viruses: global spread and genetic basis of jumping species barriers","author":"Kennedy","year":"2019","journal-title":"Viruses"},{"key":"10.1016\/j.ymeth.2026.10.003_b0185","doi-asserted-by":"crossref","unstructured":"T.A. Seimon, D.G. Miquelle, T.Y. Chang, A.L. Newton, I. Korotkova, G. Ivanchuk, E. Lyubchenko, A. Tupikov, E. Slabe, D. McAloose, Canine distemper virus: an emerging disease in wild endangered Amur tigers (Panthera tigris altaica), mBio 4 (2013).","DOI":"10.1128\/mBio.00410-13"},{"key":"10.1016\/j.ymeth.2026.10.003_b0200","doi-asserted-by":"crossref","first-page":"170","DOI":"10.7589\/2017-03-065","article-title":"Canine distemper virus in a wild far eastern leopard (panthera pardus orientalis)","volume":"54","author":"Sulikhan","year":"2018","journal-title":"J. Wildl. Dis."},{"key":"10.1016\/j.ymeth.2026.10.003_b0020","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens12020203","article-title":"Canine distemper virus in tigers (Panthera tigris) and leopards (P. pardus) in Nepal","volume":"12","author":"Bodgener","year":"2023","journal-title":"Pathogens"},{"key":"10.1016\/j.ymeth.2026.10.003_b0160","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/s12985-019-1136-6","article-title":"Tropism and molecular pathogenesis of canine distemper virus","volume":"16","author":"Rendon-Marin","year":"2019","journal-title":"Virol. J."},{"key":"10.1016\/j.ymeth.2026.10.003_b0005","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1004880","article-title":"Sequential conformational changes in the morbillivirus attachment protein initiate the membrane fusion process","volume":"11","author":"Ader-Ebert","year":"2015","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.ymeth.2026.10.003_b0080","doi-asserted-by":"crossref","first-page":"4158","DOI":"10.1128\/JVI.02647-06","article-title":"Canine distemper virus infection requires cholesterol in the viral envelope","volume":"81","author":"Imhoff","year":"2007","journal-title":"J. Virol."},{"key":"10.1016\/j.ymeth.2026.10.003_b0215","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens10010051","article-title":"Canine distemper outbreak by natural infection in a group of vaccinated maned wolves in captivity","volume":"10","author":"Vergara-Wilson","year":"2021","journal-title":"Pathogens"},{"key":"10.1016\/j.ymeth.2026.10.003_b0010","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.vetmic.2018.03.014","article-title":"Antigenic analysis of genetic variants of Canine distemper virus","volume":"219","author":"Anis","year":"2018","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.ymeth.2026.10.003_b0040","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.02077-16","article-title":"Inactivated recombinant rabies viruses displaying canine distemper virus glycoproteins induce protective immunity against both pathogens","volume":"91","author":"da Fontoura Budaszewski","year":"2017","journal-title":"J. Virol."},{"key":"10.1016\/j.ymeth.2026.10.003_b0135","doi-asserted-by":"crossref","first-page":"3811","DOI":"10.1007\/s00253-013-4831-z","article-title":"Optimisation of signal peptide for recombinant protein secretion in bacterial hosts","volume":"97","author":"Low","year":"2013","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ymeth.2026.10.003_b0235","unstructured":"R. Young, J. Rance, Mammalian expression vector with a highly efficient secretory signal sequence (Google Patents) (2012)."},{"key":"10.1016\/j.ymeth.2026.10.003_b0065","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1021\/bi00429a001","article-title":"Signal sequences","volume":"28","author":"Gierasch","year":"1989","journal-title":"Biochemistry"},{"key":"10.1016\/j.ymeth.2026.10.003_b0205","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1093\/protein\/15.4.337","article-title":"Engineering a novel secretion signal for cross-host recombinant protein expression","volume":"15","author":"Tan","year":"2002","journal-title":"Protein Eng."},{"key":"10.1016\/j.ymeth.2026.10.003_b0220","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/0022-2836(85)90046-4","article-title":"Signal sequences. The limits of variation","volume":"184","author":"von Heijne","year":"1985","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.ymeth.2026.10.003_b0155","doi-asserted-by":"crossref","first-page":"6978","DOI":"10.1093\/nar\/gks309","article-title":"Chimeric piggyBac transposases for genomic targeting in human cells","volume":"40","author":"Owens","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ymeth.2026.10.003_b0230","doi-asserted-by":"crossref","first-page":"15008","DOI":"10.1073\/pnas.0606979103","article-title":"piggyBac is a flexible and highly active transposon as compared to sleeping beauty, Tol2, and Mos1 in mammalian cells","volume":"103","author":"Wu","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ymeth.2026.10.003_b0150","doi-asserted-by":"crossref","first-page":"9197","DOI":"10.1093\/nar\/gkt677","article-title":"Transcription activator like effector (TALE)-directed piggyBac transposition in human cells","volume":"41","author":"Owens","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ymeth.2026.10.003_b0085","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1126\/science.1225829","article-title":"A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity","volume":"337","author":"Jinek","year":"2012","journal-title":"Science"},{"key":"10.1016\/j.ymeth.2026.10.003_b0100","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1038\/nrg3686","article-title":"A guide to genome engineering with programmable nucleases","volume":"15","author":"Kim","year":"2014","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.ymeth.2026.10.003_b0115","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1126\/science.aat5011","article-title":"CRISPR-Cas guides the future of genetic engineering","volume":"361","author":"Knott","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.ymeth.2026.10.003_b0175","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/nbt.2842","article-title":"CRISPR-Cas systems for editing, regulating and targeting genomes","volume":"32","author":"Sander","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.ymeth.2026.10.003_b0070","doi-asserted-by":"crossref","DOI":"10.1096\/fj.202001830RR","article-title":"CRISPR\/dCas9-mediated transposition with specificity and efficiency of site-directed genomic insertions","volume":"35","author":"Goshayeshi","year":"2021","journal-title":"FASEB J."},{"key":"10.1016\/j.ymeth.2026.10.003_b0210","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1038\/nbt.2908","article-title":"Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing","volume":"32","author":"Tsai","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.ymeth.2026.10.003_b0105","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1038\/nature16526","article-title":"High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects","volume":"529","author":"Kleinstiver","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.ymeth.2026.10.003_b0060","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.sbi.2017.10.015","article-title":"Molecular architectures and mechanisms of Class 2 CRISPR-associated nucleases","volume":"47","author":"Garcia-Doval","year":"2017","journal-title":"Curr. Opin. Struct. Biol."},{"key":"10.1016\/j.ymeth.2026.10.003_b0240","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1073\/pnas.1008322108","article-title":"A hyperactive piggyBac transposase for mammalian applications","volume":"108","author":"Yusa","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ymeth.2026.10.003_b0125","first-page":"E2279","article-title":"piggyBac transposase tools for genome engineering","volume":"110","author":"Li","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ymeth.2026.10.003_b0120","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1002\/bit.24776","article-title":"Optimized signal peptides for the development of high expressing CHO cell lines","volume":"110","author":"Kober","year":"2013","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.ymeth.2026.10.003_b0165","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1186\/s12985-015-0445-7","article-title":"Sequencing of emerging canine distemper virus strain reveals new distinct genetic lineage in the United States associated with disease in wildlife and domestic canine populations","volume":"12","author":"Riley","year":"2015","journal-title":"Virol. J."},{"key":"10.1016\/j.ymeth.2026.10.003_b0245","doi-asserted-by":"crossref","first-page":"8132","DOI":"10.1038\/s41598-017-08462-4","article-title":"Outbreak and genotyping of canine distemper virus in captive Siberian tigers and red pandas","volume":"7","author":"Zhang","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ymeth.2026.10.003_b0055","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.copbio.2004.06.004","article-title":"Prokaryotic expression of antibodies and affibodies","volume":"15","author":"Fernandez","year":"2004","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.ymeth.2026.10.003_b0095","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1111\/lam.12695","article-title":"Yeast as an expression system for producing virus-like particles: what factors do we need to consider?","volume":"64","author":"Kim","year":"2017","journal-title":"Lett. Appl. Microbiol."},{"key":"10.1016\/j.ymeth.2026.10.003_b0050","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1002\/biot.201400438","article-title":"The baculovirus expression vector system: a commercial manufacturing platform for viral vaccines and gene therapy vectors","volume":"10","author":"Felberbaum","year":"2015","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.ymeth.2026.10.003_b0035","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.pep.2015.10.012","article-title":"High-efficiency secretory expression of human neutrophil gelatinase-associated lipocalin from mammalian cell lines with human serum albumin signal peptide","volume":"118","author":"Chen","year":"2016","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.ymeth.2026.10.003_b0030","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1016\/j.yexcr.2011.02.013","article-title":"Introduction to current and future protein therapeutics: a protein engineering perspective","volume":"317","author":"Carter","year":"2011","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.ymeth.2026.10.003_b0195","doi-asserted-by":"crossref","first-page":"371","DOI":"10.3109\/07388551.2016.1163323","article-title":"Fcgamma1 fragment of IgG1 as a powerful affinity tag in recombinant Fc-fusion proteins: immunological, biochemical and therapeutic properties","volume":"37","author":"Soleimanpour","year":"2017","journal-title":"Crit. Rev. Biotechnol."},{"key":"10.1016\/j.ymeth.2026.10.003_b0015","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a007526","article-title":"Protein folding and quality control in the ER","volume":"3","author":"Araki","year":"2011","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"10.1016\/j.ymeth.2026.10.003_b0110","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.jbiotec.2006.11.026","article-title":"The level of synthesis and secretion of Gaussia princeps luciferase in transfected CHO cells is heavily dependent on the choice of signal peptide","volume":"128","author":"Knappskog","year":"2007","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.ymeth.2026.10.003_b0130","article-title":"The PAR2 signal peptide prevents premature receptor cleavage and activation","volume":"15","author":"Liu","year":"2020","journal-title":"PLoS One"},{"key":"10.1016\/j.ymeth.2026.10.003_b0180","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1247\/csf.13.129","article-title":"Secretion of human EGF and IgE in mammalian cells by recombinant DNA techniques; use of a IL-2 leader sequence","volume":"13","author":"Sasada","year":"1988","journal-title":"Cell Struct. Funct."},{"key":"10.1016\/j.ymeth.2026.10.003_b0145","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1007\/s10529-020-03072-z","article-title":"Improved secretion of recombinant human IL-25 in HEK293 cells using a signal peptide-pro-peptide domain derived from Trypsin-1","volume":"43","author":"Mullin","year":"2021","journal-title":"Biotechnol. Lett"},{"key":"10.1016\/j.ymeth.2026.10.003_b0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.jviromet.2024.115098","article-title":"Expression and characterization of canine distemper virus hemagglutinin protein in suspension mammalian cells","volume":"333","author":"Cai","year":"2025","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.ymeth.2026.10.003_b0170","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/nrc3179","article-title":"Safe harbours for the integration of new DNA in the human genome","volume":"12","author":"Sadelain","year":"2011","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.ymeth.2026.10.003_b0190","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1634\/stemcells.2007-0039","article-title":"Robust, persistent transgene expression in human embryonic stem cells is achieved with AAVS1-targeted integration","volume":"26","author":"Smith","year":"2008","journal-title":"Stem Cells"},{"key":"10.1016\/j.ymeth.2026.10.003_b0075","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/978-1-0716-2301-5_6","article-title":"Targeting the AAVS1 site by CRISPR\/Cas9 with an inducible transgene cassette for the neuronal differentiation of human pluripotent stem cells","volume":"2495","author":"Gu","year":"2022","journal-title":"Methods Mol. Biol."}],"container-title":["Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202326002082?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046202326002082?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T07:05:10Z","timestamp":1792134310000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046202326002082"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":49,"alternative-id":["S1046202326002082"],"URL":"https:\/\/doi.org\/10.1016\/j.ymeth.2026.10.003","relation":{},"ISSN":["1046-2023"],"issn-type":[{"value":"1046-2023","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Site-specific integration of canine distemper virus hemagglutinin protein in Expi293F cell lines contributes to protective neutralizing antibody responses in mice and dogs","name":"articletitle","label":"Article Title"},{"value":"Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymeth.2026.10.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}