{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:41:52Z","timestamp":1791294112667,"version":"4.1.0"},"reference-count":57,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2004,8,1]],"date-time":"2004-08-01T00:00:00Z","timestamp":1091318400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,10,7]],"date-time":"2016-10-07T00:00:00Z","timestamp":1475798400000},"content-version":"vor","delay-in-days":4450,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Therapy"],"published-print":{"date-parts":[[2004,8]]},"DOI":"10.1016\/j.ymthe.2004.05.023","type":"journal-article","created":{"date-parts":[[2004,7,7]],"date-time":"2004-07-07T15:50:37Z","timestamp":1089215437000},"page":"373-385","source":"Crossref","is-referenced-by-count":140,"title":["Macropinocytosis of Polyplexes and Recycling of Plasmid via the Clathrin-Dependent Pathway Impair the Transfection Efficiency of Human Hepatocarcinoma Cells"],"prefix":"10.1016","volume":"10","author":[{"given":"Christine","family":"Gon\u00e7alves","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Mennesson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renate","family":"Fuchs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Gorvel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Midoux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Pichon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymthe.2004.05.023_BIB1","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0169-409X(01)00213-7","article-title":"Towards synthetic viruses","volume":"52","author":"Zuber","year":"2001","journal-title":"Adv. Drug Delivery Rev"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB2","series-title":"Pharmaceutical Perspectives of Nucleic Acid-Based Therapeutics","first-page":"302","article-title":"Cationic lipid-based gene delivery","author":"Byk","year":"2002"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB3","series-title":"Pharmaceutical Perspectives of Nucleic Acid-Based Therapeutics","first-page":"334","article-title":"Peptide-based gene delivery systems","author":"Midoux","year":"2002"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB4","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1152\/physrev.1997.77.3.759","article-title":"Endocytosis","volume":"77","author":"Mukherjee","year":"1997","journal-title":"Physiol. Rev"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB5","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/S0092-8674(02)00751-1","article-title":"Clathrin adaptors really adapt","volume":"109","author":"Kirchhausen","year":"2002","journal-title":"Cell"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB6","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0169-409X(01)00221-6","article-title":"Histidine-rich peptides and polymers for nucleic acids delivery","volume":"53","author":"Pichon","year":"2001","journal-title":"Adv. Drug Delivery Rev"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB7","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1021\/bc9801070","article-title":"Efficient gene transfer by histidylated polylysine\/pDNA complexes","volume":"10","author":"Midoux","year":"1999","journal-title":"Bioconjugate Chem"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB8","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1021\/bc0000738","article-title":"Histidylated polylysine as DNA vector: elevation of the imidazole protonation and reduced cellular uptake without change in the polyfection efficiency of serum stabilized negative polyplexes","volume":"12","author":"Bello Roufai","year":"2001","journal-title":"Bioconjugate Chem"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB9","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1091\/mbc.10.4.961","article-title":"Extraction of cholesterol with methyl-beta-cyclodextrin perturbs formation of clathrin-coated endocytic vesicles","volume":"10","author":"Rodal","year":"1999","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB10","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1083\/jcb.127.5.1217","article-title":"Filipin-sensitive caveolae-mediated transport in endothelium: reduced transcytosis, scavenger endocytosis, and capillary permeability of select macromolecules","volume":"127","author":"Schnitzer","year":"1994","journal-title":"J. Cell Biol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB11","doi-asserted-by":"crossref","first-page":"6382","DOI":"10.1016\/S0021-9258(19)39337-8","article-title":"Selective modulation of the endocytic uptake of ricin and fluid phase markers without alteration in transferrin endocytosis","volume":"265","author":"Sandvig","year":"1990","journal-title":"J. Biol. Chem"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB12","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1083\/jcb.127.5.1199","article-title":"Regulated internalization of caveolae","volume":"127","author":"Parton","year":"1994","journal-title":"J. Cell Biol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB13","doi-asserted-by":"crossref","first-page":"20332","DOI":"10.1074\/jbc.272.33.20332","article-title":"The actin cytoskeleton is required for receptor-mediated endocytosis in mammalian cells","volume":"272","author":"Lamaze","year":"1997","journal-title":"J. Biol. Chem"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB14","doi-asserted-by":"crossref","first-page":"2441","DOI":"10.1242\/jcs.110.19.2441","article-title":"Microtubule depolymerization inhibits clathrin coated-pit internalization in non-adherent cell lines while interleukin 2 endocytosis is not affected","volume":"110","author":"Subtil","year":"1997","journal-title":"J. Cell Sci"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB15","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1006\/excr.1995.1075","article-title":"Phorbol myristate acetate selectively stimulates apical endocytosis via protein kinase C in polarized MDCK cells","volume":"217","author":"Holm","year":"1995","journal-title":"Exp. Cell Res"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB16","doi-asserted-by":"crossref","first-page":"3453","DOI":"10.1091\/mbc.11.10.3453","article-title":"Constitutive macropinocytosis in oncogene-transformed fibroblasts depends on sequential permanent activation of phosphoinositide 3-kinase and phospholipase C","volume":"11","author":"Amyere","year":"2000","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB17","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/S0092-8674(00)80461-4","article-title":"Molecular mechanisms of endocytosis","volume":"91","author":"Riezman","year":"1997","journal-title":"Cell"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB18","doi-asserted-by":"crossref","first-page":"2953","DOI":"10.1242\/jcs.115.14.2953","article-title":"Membrane ruffling and macropinocytosis in A431 cells require cholesterol","volume":"115","author":"Grimmer","year":"2002","journal-title":"J. Cell Sci"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB19","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1083\/jcb.135.5.1249","article-title":"A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages","volume":"135","author":"Araki","year":"1996","journal-title":"J. Cell Biol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB20","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1083\/jcb.132.4.595","article-title":"Potential sites of PI-3 kinase function in the endocytic pathway revealed by the PI-3 kinase inhibitor, wortmannin","volume":"132","author":"Shpetner","year":"1996","journal-title":"J. Cell Biol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB21","doi-asserted-by":"crossref","first-page":"2173","DOI":"10.1099\/0022-1317-77-9-2173","article-title":"Extracellular simian virus 40 induces an ERK\/MAP kinase-independent signalling pathway that activates primary response genes and promotes virus entry","volume":"77","author":"Dangoria","year":"1996","journal-title":"J. Gen. Virol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB22","doi-asserted-by":"crossref","first-page":"2927","DOI":"10.1242\/jcs.109.12.2927","article-title":"The effects of cytochalasin D and phorbol myristate acetate on the apical endocytosis of ricin in polarised Caco-2 cells","volume":"109","author":"Shurety","year":"1996","journal-title":"J. Cell Sci"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB23","doi-asserted-by":"crossref","first-page":"3509","DOI":"10.1242\/jcs.113.19.3509","article-title":"Isoforms of caveolin-1 and caveolar structure","volume":"113","author":"Fujimoto","year":"2000","journal-title":"J. Cell Sci"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB24","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1083\/jcb.200102084","article-title":"Clathrin-dependent and -independent internalization of plasma membrane sphingolipids initiates two Golgi targeting pathways","volume":"154","author":"Puri","year":"2001","journal-title":"J. Cell Biol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB25","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1006\/bbrc.1995.1846","article-title":"Inhibitors of phosphoinositide 3-kinase block exocytosis but not endocytosis of transferrin receptors in 3T3-L1 adipocytes","volume":"211","author":"Shepherd","year":"1995","journal-title":"Biochem. Biophys. Res. Commun"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB26","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1083\/jcb.141.4.905","article-title":"Filipin-dependent inhibition of cholera toxin: evidence for toxin internalization and activation through caveolae-like domains","volume":"141","author":"Orlandi","year":"1998","journal-title":"J. Cell Biol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB27","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/S0962-8924(01)02107-9","article-title":"Endocytosis without clathrin coats","volume":"11","author":"Nichols","year":"2001","journal-title":"Trends Cell Biol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB28","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1002\/jgm.277","article-title":"Intracellular processing and stability of DNA complexed with histidylated polylysine conjugates","volume":"4","author":"Goncalves","year":"2002","journal-title":"J. Gene Med"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB29","doi-asserted-by":"crossref","first-page":"2682","DOI":"10.1002\/elps.1150181425","article-title":"Flow cytometric sorting and biochemical characterization of the late endosomal rab7-containing compartment","volume":"18","author":"Meresse","year":"1997","journal-title":"Electrophoresis"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB30","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1083\/jcb.106.6.1821","article-title":"The pathways of endocytosed transferrin and secretory protein are connected in the trans-Golgi reticulum","volume":"106","author":"Stoorvogel","year":"1988","journal-title":"J. Cell Biol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB31","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1016\/S0021-9258(18)52307-3","article-title":"Trafficking of the epidermal growth factor receptor and transferrin in three hepatocytic endosomal fractions","volume":"266","author":"Jackle","year":"1991","journal-title":"J. Biol. Chem"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB32","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1083\/jcb.137.5.1161","article-title":"Different steady state subcellular distributions of the three splice variants of lysosome-associated membrane protein LAMP-2 are determined largely by the COOH-terminal amino acid residue","volume":"137","author":"Gough","year":"1997","journal-title":"J. Cell Biol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB33","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1034\/j.1600-0854.2001.020301.x","article-title":"Endocytic traffic in polarized epithelial cells: role of the actin and microtubule cytoskeleton","volume":"2","author":"Apodaca","year":"2001","journal-title":"Traffic"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB34","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1242\/jcs.1993.Supplement_17.10","article-title":"The cytoskeleton in development of epithelial cell polarity","volume":"17","author":"Fath","year":"1993","journal-title":"J. Cell Sci"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB35","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1083\/jcb.135.4.913","article-title":"Rab11 regulates recycling through the pericentriolar recycling endosome","volume":"135","author":"Ullrich","year":"1996","journal-title":"J. Cell Biol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB36","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1006\/bbrc.1997.7520","article-title":"Rab11 is associated with transferrin-containing recycling compartments in K562 cells","volume":"239","author":"Green","year":"1997","journal-title":"Biochem. Biophys. Res. Commun"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB37","doi-asserted-by":"crossref","first-page":"6187","DOI":"10.1073\/pnas.95.11.6187","article-title":"Hydrolysis of GTP on rab11 is required for the direct delivery of transferrin from the pericentriolar recycling compartment to the cell surface but not from sorting endosomes","volume":"95","author":"Ren","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB38","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1242\/jcs.112.9.1303","article-title":"Inhibition of clathrin-coated pit assembly by an Eps15 mutant","volume":"112","author":"Benmerah","year":"1999","journal-title":"J. Cell Sci"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB39","first-page":"147","article-title":"Cationic liposomes as gene transfer vectors: barriers to successful application in gene therapy","volume":"1","author":"Simoes","year":"1999","journal-title":"Curr. Opin. Mol. Ther"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB40","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1042\/bj20031253","article-title":"Size-dependent internalization of particles via the pathways of clathrin- and caveolae-mediated endocytosis","volume":"377","author":"Rejman","year":"2004","journal-title":"Biochem. J"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB41","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1021\/bc0255182","article-title":"Intracellular delivery of nanomeric DNA particles via the folate receptor","volume":"13","author":"Dauty","year":"2002","journal-title":"Bioconjugate Chem"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB42","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1002\/jgm.318","article-title":"Uptake of plasmid\/glycosylated polymer complexes and gene transfer efficiency in differentiated airway epithelial cells","volume":"5","author":"Fajac","year":"2003","journal-title":"J. Gene Med"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB43","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S1525-0016(02)00016-3","article-title":"Lactosylated poly-l-lysine targets a potential lactose receptor in cystic fibrosis and non-cystic fibrosis airway epithelial cells","volume":"7","author":"Klink","year":"2003","journal-title":"Mol. Ther"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB44","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1084\/jem.182.2.389","article-title":"Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: downregulation by cytokines and bacterial products","volume":"182","author":"Sallusto","year":"1995","journal-title":"J. Exp. Med"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB45","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.1242\/jcs.109.8.2005","article-title":"v-Src induces constitutive macropinocytosis in rat fibroblasts","volume":"109","author":"Veithen","year":"1996","journal-title":"J. Cell Sci"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB46","doi-asserted-by":"crossref","first-page":"6689","DOI":"10.1128\/JVI.76.13.6689-6700.2002","article-title":"Human immunodeficiency virus type 1 enters brain microvascular endothelia by macropinocytosis dependent on lipid rafts and the mitogen-activated protein kinase signaling pathway","volume":"76","author":"Liu","year":"2002","journal-title":"J. Virol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB47","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1046\/j.1462-5822.2002.00195.x","article-title":"Modulation of Brucella-induced macropinocytosis by lipid rafts mediates intracellular replication","volume":"4","author":"Watarai","year":"2002","journal-title":"Cell. Microbiol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB48","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1083\/jcb.124.5.689","article-title":"The coated pit and macropinocytic pathways serve distinct endosome populations","volume":"124","author":"Hewlett","year":"1994","journal-title":"J. Cell Biol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB49","doi-asserted-by":"crossref","first-page":"44826","DOI":"10.1074\/jbc.M308643200","article-title":"Chloride accumulation and swelling in endosomes enhances DNA transfer by polyamine\u2013DNA polyplexes","volume":"278","author":"Sonawane","year":"2003","journal-title":"J. Biol. Chem"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB50","first-page":"49","article-title":"Phagosome maturation and fusion with lysosomes in relation to surface property and size of the phagocytic particle","volume":"74","author":"De Chastellier","year":"1997","journal-title":"Eur. J. Cell Biol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB51","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1083\/jcb.124.5.677","article-title":"Biogenesis of phagolysosomes proceeds through a sequential series of interactions with the endocytic apparatus","volume":"124","author":"Desjardins","year":"1994","journal-title":"J. Cell Biol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB52","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1242\/jcs.94.1.135","article-title":"Phorbol esters stimulate macropinocytosis and solute flow through macrophages","volume":"94","author":"Swanson","year":"1989","journal-title":"J. Cell Sci"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB53","doi-asserted-by":"crossref","first-page":"18021","DOI":"10.1074\/jbc.M111257200","article-title":"Lipoplex-mediated transfection of mammalian cells occurs through the cholesterol-dependent clathrin-mediated pathway of endocytosis","volume":"277","author":"Zuhorn","year":"2002","journal-title":"J. Biol. Chem"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB54","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/0014-4827(77)90089-1","article-title":"In situ detection of mycoplasma contamination in cell culture by fluorescent Hoechst 33258 stain","volume":"104","author":"Chen","year":"1977","journal-title":"Exp. Cell Res"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB55","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1083\/jcb.121.1.61","article-title":"Clathrin and HA2 adaptors: effects of potassium depletion, hypertonic medium, and cytosol acidification","volume":"121","author":"Hansen","year":"1993","journal-title":"J. Cell Biol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB56","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1083\/jcb.105.2.679","article-title":"Acidification of the cytosol inhibits endocytosis from coated pits","volume":"105","author":"Sandvig","year":"1987","journal-title":"J. Cell Biol"},{"key":"10.1016\/j.ymthe.2004.05.023_BIB57","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1002\/cyto.990080314","article-title":"Quantitation of the binding, uptake, and degradation of fluoresceinylated neoglycoproteins by flow cytometry","volume":"8","author":"Midoux","year":"1987","journal-title":"Cytometry"}],"container-title":["Molecular Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S152500160400190X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S152500160400190X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T01:53:13Z","timestamp":1624499593000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S152500160400190X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,8]]},"references-count":57,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,8]]}},"alternative-id":["S152500160400190X"],"URL":"https:\/\/doi.org\/10.1016\/j.ymthe.2004.05.023","relation":{},"ISSN":["1525-0016"],"issn-type":[{"value":"1525-0016","type":"print"}],"subject":[],"published":{"date-parts":[[2004,8]]}}}