{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:29:24Z","timestamp":1791163764011,"version":"4.1.0"},"reference-count":24,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2005,9,1]],"date-time":"2005-09-01T00:00:00Z","timestamp":1125532800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,10,7]],"date-time":"2016-10-07T00:00:00Z","timestamp":1475798400000},"content-version":"vor","delay-in-days":4054,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Therapy"],"published-print":{"date-parts":[[2005,9]]},"DOI":"10.1016\/j.ymthe.2005.03.002","type":"journal-article","created":{"date-parts":[[2005,4,21]],"date-time":"2005-04-21T11:42:17Z","timestamp":1114083737000},"page":"493-501","source":"Crossref","is-referenced-by-count":61,"title":["Aerosolized nanogram quantities of plasmid DNA mediate highly efficient gene delivery to mouse airway epithelium"],"prefix":"10.1016","volume":"12","author":[{"given":"Carsten","family":"Rudolph","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Schillinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurora","family":"Ortiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Plank","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika M.","family":"Golas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bjoern","family":"Sander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Stark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Rosenecker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymthe.2005.03.002_bib1","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1006\/mthe.1999.0021","article-title":"Aerosol delivery of robust polyethyleneimine\u2013DNA complexes for gene therapy and genetic immunization","volume":"1","author":"Densmore","year":"2000","journal-title":"Mol. Ther."},{"key":"10.1016\/j.ymthe.2005.03.002_bib2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1002\/jgm.646","article-title":"Methodological optimization of polyethylenimine (PEI)-based gene delivery to the lungs of mice via aerosol application","volume":"7","author":"Rudolph","year":"2005","journal-title":"J. Gene Med."},{"key":"10.1016\/j.ymthe.2005.03.002_bib3","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1038\/sj.gt.3300745","article-title":"The size of DNA\/transferrin\u2013PEI complexes is an important factor for gene expression in cultured cells","volume":"5","author":"Ogris","year":"1998","journal-title":"Gene Ther."},{"key":"10.1016\/j.ymthe.2005.03.002_bib4","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1002\/jgm.187","article-title":"Different behavior of branched and linear polyethylenimine for gene delivery in vitro and in vivo","volume":"3","author":"Wightman","year":"2001","journal-title":"J. Gene Med."},{"key":"10.1016\/j.ymthe.2005.03.002_bib5","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1152\/jappl.1996.81.1.491","article-title":"Transient permeabilization of airway epithelium by mucosal water","volume":"81","author":"Widdicombe","year":"1996","journal-title":"J. Appl. Physiol."},{"key":"10.1016\/j.ymthe.2005.03.002_bib6","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1038\/sj.gt.3301227","article-title":"Protective copolymers for nonviral gene vectors: synthesis, vector characterization and application in gene delivery","volume":"7","author":"Finsinger","year":"2000","journal-title":"Gene Ther."},{"key":"10.1016\/j.ymthe.2005.03.002_bib7","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1006\/mthe.2002.0568","article-title":"Importance of lateral and steric stabilization of polyelectrolyte gene delivery vectors for extended systemic circulation","volume":"5","author":"Oupicky","year":"2002","journal-title":"Mol. Ther."},{"key":"10.1016\/j.ymthe.2005.03.002_bib8","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1152\/jappl.1999.86.5.1505","article-title":"Effect of ultrasonically nebulized distilled water on airway epithelial cell swelling in guinea pigs","volume":"86","author":"Mochizuki","year":"1999","journal-title":"J. Appl. Physiol."},{"key":"10.1016\/j.ymthe.2005.03.002_bib9","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1002\/(SICI)1521-2254(199905\/06)1:3<210::AID-JGM30>3.0.CO;2-U","article-title":"Transfection and physical properties of various saccharide, poly(ethylene glycol), and antibody-derivatized polyethylenimines (PEI)","volume":"1","author":"Erbacher","year":"1999","journal-title":"J. Gene Med."},{"key":"10.1016\/j.ymthe.2005.03.002_bib10","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1002\/(SICI)1521-2254(199909\/10)1:5<331::AID-JGM60>3.0.CO;2-8","article-title":"Interaction of liposomal and polycationic transfection complexes with pulmonary surfactant","volume":"1","author":"Ernst","year":"1999","journal-title":"J. Gene Med."},{"key":"10.1016\/j.ymthe.2005.03.002_bib11","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1152\/jappl.2002.92.1.155","article-title":"Effect of inhaled indomethacin on distilled water-induced airway epithelial cell swelling","volume":"92","author":"Mochizuki","year":"2002","journal-title":"J. Appl. Physiol."},{"key":"10.1016\/j.ymthe.2005.03.002_bib12","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1089\/hum.1996.7.8-933","article-title":"Intraluminal water increases expression of plasmid DNA in rat lung","volume":"7","author":"Sawa","year":"1996","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.ymthe.2005.03.002_bib13","first-page":"L277","article-title":"Ozone reduces murine alveolar and peritoneal macrophage phagocytosis: the role of prostanoids","volume":"261","author":"Canning","year":"1991","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.ymthe.2005.03.002_bib14","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1165\/ajrcmb.22.2.3938","article-title":"Increasing epithelial junction permeability enhances gene transfer to airway epithelia in vivo","volume":"22","author":"Wang","year":"2000","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"10.1016\/j.ymthe.2005.03.002_bib15","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/104303401300042348","article-title":"EGTA enhancement of adenovirus-mediated gene transfer to mouse tracheal epithelium in vivo","volume":"12","author":"Chu","year":"2001","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.ymthe.2005.03.002_bib16","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1089\/hum.1998.9.18-2661","article-title":"Enhanced in vivo airway gene transfer via transient modification of host barrier properties with a surface-active agent","volume":"9","author":"Parsons","year":"1998","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.ymthe.2005.03.002_bib17","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/S1525-0016(03)00177-1","article-title":"Biodistribution and pharmacokinetics of aerosol and intravenously administered DNA\u2013polyethyleneimine complexes: optimization of pulmonary delivery and retention","volume":"8","author":"Koshkina","year":"2003","journal-title":"Mol. Ther."},{"key":"10.1016\/j.ymthe.2005.03.002_bib18","first-page":"424","article-title":"A specifically induced bronchoconstriction by distilled water in children and adults","volume":"30","author":"Castello","year":"1980","journal-title":"Bronchopneumologie"},{"key":"10.1016\/j.ymthe.2005.03.002_bib19","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1002\/1521-2254(200007\/08)2:4<269::AID-JGM112>3.0.CO;2-F","article-title":"In vivo gene delivery to the lung using polyethylenimine and fractured polyamidoamine dendrimers","volume":"2","author":"Rudolph","year":"2000","journal-title":"J. Gene Med."},{"key":"10.1016\/j.ymthe.2005.03.002_bib20","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.1021\/bi00846a017","article-title":"Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli","volume":"7","author":"Valentine","year":"1968","journal-title":"Biochemistry"},{"key":"10.1016\/j.ymthe.2005.03.002_bib21","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/BF00267715","article-title":"Determination of the location of proteins L14, L17, L18, L19, L22, L23 on the surface of the 5oS ribosomal subunit of Escherichia coli by immune electron microscopy","volume":"134","author":"Tischendorf","year":"1974","journal-title":"Mol. Gen. Genet."},{"key":"10.1016\/j.ymthe.2005.03.002_bib22","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1038\/308032a0","article-title":"Cryo-electron microscopy of viruses","volume":"308","author":"Adrian","year":"1984","journal-title":"Nature"},{"key":"10.1016\/j.ymthe.2005.03.002_bib23","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0731-7085(02)00571-X","article-title":"Spectrophotometric determination of polyethylenimine in the presence of an oligonucleotide for the characterization of controlled release formulations","volume":"31","author":"Ungaro","year":"2003","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.ymthe.2005.03.002_bib24","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1006\/abio.1998.2834","article-title":"Iodinated plasmid DNA as a tool for studying gene delivery","volume":"263","author":"Terebesi","year":"1998","journal-title":"Anal. Biochem."}],"container-title":["Molecular Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525001605000894?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525001605000894?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,2,15]],"date-time":"2019-02-15T05:06:25Z","timestamp":1550207185000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1525001605000894"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,9]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,9]]}},"alternative-id":["S1525001605000894"],"URL":"https:\/\/doi.org\/10.1016\/j.ymthe.2005.03.002","relation":{},"ISSN":["1525-0016"],"issn-type":[{"value":"1525-0016","type":"print"}],"subject":[],"published":{"date-parts":[[2005,9]]}}}