{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:19:07Z","timestamp":1792606747962,"version":"4.5.0"},"reference-count":43,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T00:00:00Z","timestamp":1593734400000},"content-version":"vor","delay-in-days":368,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006441","name":"Center for Outcomes Research and Evaluation, Yale School of Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006441","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000098","name":"NIH Clinical Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000098","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Therapy"],"published-print":{"date-parts":[[2019,7]]},"DOI":"10.1016\/j.ymthe.2019.05.015","type":"journal-article","created":{"date-parts":[[2019,5,30]],"date-time":"2019-05-30T17:00:09Z","timestamp":1559235609000},"page":"1275-1285","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":105,"title":["Effect of Cryopreservation on Autologous Chimeric Antigen Receptor T Cell Characteristics"],"prefix":"10.1016","volume":"27","author":[{"given":"Sandhya R.","family":"Panch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandeep K.","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasha","family":"Elavia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"McManus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shutong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven L.","family":"Highfill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobai","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradeep","family":"Dagur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James N.","family":"Kochenderfer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terry J.","family":"Fry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crystal L.","family":"Mackall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirali N.","family":"Shah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David F.","family":"Stroncek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymthe.2019.05.015_bib1","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1158\/1078-0432.CCR-18-2035","article-title":"FDA Approval Summary: Tisagenlecleucel for Treatment of Patients with Relapsed or Refractory B-Cell Precursor Acute Lymphoblastic Leukemia","volume":"25","author":"O\u2019Leary","year":"2019","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.ymthe.2019.05.015_bib2","first-page":"818","article-title":"Second anticancer CAR T therapy receives FDA approval","volume":"16","author":"Mullard","year":"2017","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.ymthe.2019.05.015_bib3","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1038\/s41591-018-0201-9","article-title":"Induction of resistance to chimeric antigen receptor T\u00a0cell therapy by transduction of a single leukemic B cell","volume":"24","author":"Ruella","year":"2018","journal-title":"Nat. Med."},{"key":"10.1016\/j.ymthe.2019.05.015_bib4","doi-asserted-by":"crossref","first-page":"150","DOI":"10.3389\/fmed.2018.00150","article-title":"Industrializing Autologous Adoptive Immunotherapies: Manufacturing Advances and Challenges","volume":"5","author":"Iyer","year":"2018","journal-title":"Front. Med. (Lausanne)"},{"key":"10.1016\/j.ymthe.2019.05.015_bib5","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.copbio.2018.01.025","article-title":"A guide to manufacturing CAR T\u00a0cell therapies","volume":"53","author":"Vormittag","year":"2018","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.ymthe.2019.05.015_bib6","doi-asserted-by":"crossref","first-page":"16015","DOI":"10.1038\/mto.2016.15","article-title":"Clinical manufacturing of CAR T\u00a0cells: foundation of a promising therapy","volume":"3","author":"Wang","year":"2016","journal-title":"Mol. Ther. Oncolytics"},{"key":"10.1016\/j.ymthe.2019.05.015_bib7","doi-asserted-by":"crossref","first-page":"2782","DOI":"10.1111\/trf.14360","article-title":"Cryopreservation of adult unrelated donor products in hematopoietic cell transplantation: the OneMatch experience and systematic review of the literature","volume":"57","author":"Aziz","year":"2017","journal-title":"Transfusion"},{"key":"10.1016\/j.ymthe.2019.05.015_bib8","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1038\/bmt.2017.113","article-title":"Analysis of hematopoietic recovery after autologous transplantation as method of quality control for long-term progenitor cell cryopreservation","volume":"52","author":"Pavl\u016f","year":"2017","journal-title":"Bone Marrow Transplant."},{"key":"10.1016\/j.ymthe.2019.05.015_bib9","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1016\/j.jcyt.2016.06.014","article-title":"Variable resistance to freezing and thawing of CD34-positive stem cells and lymphocyte subpopulations in leukapheresis products","volume":"18","author":"Berens","year":"2016","journal-title":"Cytotherapy"},{"key":"10.1016\/j.ymthe.2019.05.015_bib10","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1111\/trf.12428","article-title":"Analysis of the recovery of cryopreserved and thawed CD34+ and CD3+ cells collected for hematopoietic transplantation","volume":"54","author":"Fisher","year":"2014","journal-title":"Transfusion"},{"key":"10.1016\/j.ymthe.2019.05.015_bib11","doi-asserted-by":"crossref","first-page":"S62","DOI":"10.1016\/j.bbmt.2018.12.143","article-title":"Automated Manufacturing of CD20.19 Bi-Specific Chimeric Antigen Receptor T (CAR-T) Cells at an Academic Center for a Phase I Clinical Trial in Relapsed, Refractory NHL","volume":"25","author":"Zhu","year":"2019","journal-title":"Biol. Blood Marrow Transplant."},{"issue":"Suppl 1","key":"10.1016\/j.ymthe.2019.05.015_bib12","doi-asserted-by":"crossref","first-page":"4553","DOI":"10.1182\/blood-2018-99-116566","article-title":"Point-of-Care Manufacturing of CD20.19 Bi-Specific Chimeric Antigen Receptor T (CAR-T) Cells in a Standard Academic Cell Processing Facility for a Phase I Clinical Trial in Relapsed, Refractory NHL","volume":"132","author":"Zhu","year":"2018","journal-title":"Blood"},{"key":"10.1016\/j.ymthe.2019.05.015_bib13","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1038\/nbt.2786","article-title":"Clinical development success rates for investigational drugs","volume":"32","author":"Hay","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.ymthe.2019.05.015_bib14","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1038\/nrd4651","article-title":"Regulatory watch: Why do oncology drugs fail to gain US regulatory approval?","volume":"14","author":"Khozin","year":"2015","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.ymthe.2019.05.015_bib15","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1038\/s41551-018-0246-6","article-title":"Biomanufacturing for clinically advanced cell therapies","volume":"2","author":"Aijaz","year":"2018","journal-title":"Nat. Biomed. Eng."},{"key":"10.1016\/j.ymthe.2019.05.015_bib16","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1038\/nbt.3525","article-title":"Quality cell therapy manufacturing by design","volume":"34","author":"Lipsitz","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.ymthe.2019.05.015_bib17","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.stem.2016.12.003","article-title":"Achieving Efficient Manufacturing and Quality Assurance through Synthetic Cell Therapy Design","volume":"20","author":"Lipsitz","year":"2017","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.ymthe.2019.05.015_bib18","doi-asserted-by":"crossref","first-page":"2055","DOI":"10.1111\/j.1537-2995.2011.03547.x","article-title":"Cryopreservation of umbilical cord blood with a novel freezing solution that mimics intracellular ionic composition","volume":"52","author":"Nicoud","year":"2012","journal-title":"Transfusion"},{"key":"10.1016\/j.ymthe.2019.05.015_bib19","doi-asserted-by":"crossref","first-page":"3155","DOI":"10.1111\/trf.12756","article-title":"Optimization of the freezing process for hematopoietic progenitor cells: effect of precooling, initial dimethyl sulfoxide concentration, freezing program, and storage in vapor-phase or liquid nitrogen on in\u00a0vitro white blood cell quality","volume":"54","author":"Dijkstra-Tiekstra","year":"2014","journal-title":"Transfusion"},{"key":"10.1016\/j.ymthe.2019.05.015_bib20","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1089\/bio.2016.0119","article-title":"Integrating Molecular Control to Improve Cryopreservation Outcome","volume":"15","author":"Baust","year":"2017","journal-title":"Biopreserv. Biobank."},{"key":"10.1016\/j.ymthe.2019.05.015_bib21","doi-asserted-by":"crossref","first-page":"9728","DOI":"10.18632\/oncotarget.23887","article-title":"Cell banking for regulatory T\u00a0cell-based therapy: strategies to overcome the impact of cryopreservation on the Treg viability and phenotype","volume":"9","author":"Golab","year":"2018","journal-title":"Oncotarget"},{"key":"10.1016\/j.ymthe.2019.05.015_bib22","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1006\/cryo.1999.2157","article-title":"Cryobiophysical characteristics of genetically modified hematopoietic progenitor cells","volume":"38","author":"Hubel","year":"1999","journal-title":"Cryobiology"},{"key":"10.1016\/j.ymthe.2019.05.015_bib23","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1177\/096368979900800507","article-title":"Freezing characteristics of genetically modified lymphocytes for the treatment of MPS II","volume":"8","author":"Hubel","year":"1999","journal-title":"Cell Transplant."},{"key":"10.1016\/j.ymthe.2019.05.015_bib24","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1038\/nprot.2016.028","article-title":"Monitoring the progression of cell death and the disassembly of dying cells by flow cytometry","volume":"11","author":"Jiang","year":"2016","journal-title":"Nat. Protoc."},{"issue":"Suppl 1","key":"10.1016\/j.ymthe.2019.05.015_bib25","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1182\/blood-2018-197","article-title":"CD19-Directed CAR T-Cell (CTL019) Product Viability and Clinical Outcomes in Non-Hodgkin Lymphomas and B-Cell Acute Lymphoblastic Leukemia","volume":"132","author":"Chong","year":"2018","journal-title":"Blood"},{"key":"10.1016\/j.ymthe.2019.05.015_bib26","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1056\/NEJMra1706169","article-title":"Chimeric Antigen Receptor Therapy","volume":"379","author":"June","year":"2018","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.ymthe.2019.05.015_bib27","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.1016\/j.ymthe.2018.09.009","article-title":"In\u00a0Vivo Fate and Activity of Second- versus Third-Generation CD19-Specific CAR-T Cells in B Cell Non-Hodgkin\u2019s Lymphomas","volume":"26","author":"Ramos","year":"2018","journal-title":"Mol. Ther."},{"key":"10.1016\/j.ymthe.2019.05.015_bib28","doi-asserted-by":"crossref","first-page":"2123","DOI":"10.1172\/JCI85309","article-title":"CD19 CAR-T cells of defined CD4+:CD8+ composition in adult B cell ALL patients","volume":"126","author":"Turtle","year":"2016","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.ymthe.2019.05.015_bib29","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1016\/j.jcyt.2016.03.295","article-title":"Off the shelf cellular therapeutics: Factors to consider during cryopreservation and storage of human cells for clinical use","volume":"18","author":"Woods","year":"2016","journal-title":"Cytotherapy"},{"key":"10.1016\/j.ymthe.2019.05.015_bib30","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1111\/trf.14112","article-title":"Clinical methods of cryopreservation for donor lymphocyte infusions vary in their ability to preserve functional T-cell subpopulations","volume":"57","author":"Worsham","year":"2017","journal-title":"Transfusion"},{"key":"10.1016\/j.ymthe.2019.05.015_bib31","series-title":"Practical Handbook of Cell Therapy Cryopreservation","author":"Creer","year":"2015"},{"key":"10.1016\/j.ymthe.2019.05.015_bib32","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1111\/j.1537-2995.2005.00638.x","article-title":"Cell loss and recovery in umbilical cord blood processing: a comparison of postthaw and postwash samples","volume":"45","author":"Laroche","year":"2005","journal-title":"Transfusion"},{"key":"10.1016\/j.ymthe.2019.05.015_bib33","doi-asserted-by":"crossref","first-page":"2670","DOI":"10.1111\/j.1537-2995.2010.02803.x","article-title":"Comparison of cord blood thawing methods on cell recovery, potency, and infusion","volume":"50","author":"Regan","year":"2010","journal-title":"Transfusion"},{"key":"10.1016\/j.ymthe.2019.05.015_bib34","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.cryobiol.2018.06.007","article-title":"Effects of cryopreservation on chimeric antigen receptor T\u00a0cell functions","volume":"83","author":"Xu","year":"2018","journal-title":"Cryobiology"},{"key":"10.1016\/j.ymthe.2019.05.015_bib35","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1016\/j.jcyt.2017.04.005","article-title":"Transient warming events occurring after freezing impairs umbilical cord-derived mesenchymal stromal cells functionality","volume":"19","author":"Chabot","year":"2017","journal-title":"Cytotherapy"},{"key":"10.1016\/j.ymthe.2019.05.015_bib36","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1089\/bio.2013.0032","article-title":"Targeting cryopreservation-induced cell death: a review","volume":"12","author":"Bissoyi","year":"2014","journal-title":"Biopreserv. Biobank."},{"key":"10.1016\/j.ymthe.2019.05.015_bib37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cryobiol.2016.12.002","article-title":"Effects of cryopreservation on the epigenetic profile of cells","volume":"74","author":"Chatterjee","year":"2017","journal-title":"Cryobiology"},{"key":"10.1016\/j.ymthe.2019.05.015_bib38","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1016\/j.jcyt.2016.04.003","article-title":"Myeloid cells in peripheral blood mononuclear cell concentrates inhibit the expansion of chimeric antigen receptor T\u00a0cells","volume":"18","author":"Stroncek","year":"2016","journal-title":"Cytotherapy"},{"key":"10.1016\/j.ymthe.2019.05.015_bib39","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/nm.4441","article-title":"CD22-targeted CAR T\u00a0cells induce remission in B-ALL that is naive or resistant to CD19-targeted CAR immunotherapy","volume":"24","author":"Fry","year":"2018","journal-title":"Nat. Med."},{"key":"10.1016\/j.ymthe.2019.05.015_bib40","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/S0140-6736(14)61403-3","article-title":"T cells expressing CD19 chimeric antigen receptors for acute lymphoblastic leukaemia in children and young adults: a phase 1 dose-escalation trial","volume":"385","author":"Lee","year":"2015","journal-title":"Lancet"},{"key":"10.1016\/j.ymthe.2019.05.015_bib41","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1182\/blood-2016-04-711903","article-title":"T cells expressing an anti-B-cell maturation antigen chimeric antigen receptor cause remissions of multiple myeloma","volume":"128","author":"Ali","year":"2016","journal-title":"Blood"},{"key":"10.1016\/j.ymthe.2019.05.015_bib42","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1200\/JCO.2015.64.5929","article-title":"Allogeneic T Cells That Express an Anti-CD19 Chimeric Antigen Receptor Induce Remissions of B-Cell Malignancies That Progress After Allogeneic Hematopoietic Stem-Cell Transplantation Without Causing Graft-Versus-Host Disease","volume":"34","author":"Brudno","year":"2016","journal-title":"J.\u00a0Clin. Oncol."},{"key":"10.1016\/j.ymthe.2019.05.015_bib43","doi-asserted-by":"crossref","first-page":"46731","DOI":"10.1038\/srep46731","article-title":"Manufacturing Differences Affect Human Bone Marrow Stromal Cell Characteristics and Function: Comparison of Production Methods and Products from Multiple Centers","volume":"7","author":"Liu","year":"2017","journal-title":"Sci. Rep."}],"container-title":["Molecular Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525001619302497?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525001619302497?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T17:55:20Z","timestamp":1761501320000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1525001619302497"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7]]},"references-count":43,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["S1525001619302497"],"URL":"https:\/\/doi.org\/10.1016\/j.ymthe.2019.05.015","relation":{},"ISSN":["1525-0016"],"issn-type":[{"value":"1525-0016","type":"print"}],"subject":[],"published":{"date-parts":[[2019,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Effect of Cryopreservation on Autologous Chimeric Antigen Receptor T Cell Characteristics","name":"articletitle","label":"Article Title"},{"value":"Molecular Therapy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymthe.2019.05.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"name":"copyright","label":"Copyright"}]}}