{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T21:11:52Z","timestamp":1790284312448,"version":"4.1.0"},"reference-count":89,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T00:00:00Z","timestamp":1688169600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T00:00:00Z","timestamp":1688169600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T00:00:00Z","timestamp":1678320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001108","name":"Ophthalmic Research Institute of Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001230","name":"Macquarie University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001230","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Therapy"],"published-print":{"date-parts":[[2023,7]]},"DOI":"10.1016\/j.ymthe.2023.03.008","type":"journal-article","created":{"date-parts":[[2023,3,11]],"date-time":"2023-03-11T10:47:37Z","timestamp":1678531657000},"page":"2056-2076","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":29,"title":["Neuroserpin gene therapy inhibits retinal ganglion cell apoptosis and promotes functional preservation in glaucoma"],"prefix":"10.1016","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8038-4387","authenticated-orcid":false,"given":"Nitin","family":"Chitranshi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashi","family":"Rajput","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Godinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanishka","family":"Pushpitha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Mirzaei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devaraj","family":"Basavarajappa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veer","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samridhi","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyi","family":"You","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Galliciotti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghasem H.","family":"Salekdeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark S.","family":"Baker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart L.","family":"Graham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivek K.","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymthe.2023.03.008_bib1","doi-asserted-by":"crossref","first-page":"e144","DOI":"10.1016\/S2214-109X(20)30489-7","article-title":"Causes of blindness and vision impairment in 2020 and trends over 30 years, and prevalence of avoidable blindness in relation to VISION 2020: the Right to Sight: an analysis for the Global Burden of Disease Study","volume":"9","year":"2021","journal-title":"Lancet. Glob. Health"},{"key":"10.1016\/j.ymthe.2023.03.008_bib2","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1111\/j.1442-9071.2012.02773.x","article-title":"Definition of glaucoma: clinical and experimental concepts","volume":"40","author":"Casson","year":"2012","journal-title":"Clin. Exp. Ophthalmol."},{"key":"10.1016\/j.ymthe.2023.03.008_bib3","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1001\/jama.2014.3192","article-title":"The pathophysiology and treatment of glaucoma: a review","volume":"311","author":"Weinreb","year":"2014","journal-title":"JAMA"},{"key":"10.1016\/j.ymthe.2023.03.008_bib4","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/bs.pbr.2015.04.003","article-title":"The contribution of the sclera and lamina cribrosa to the pathogenesis of glaucoma: diagnostic and treatment implications","volume":"220","author":"Quigley","year":"2015","journal-title":"Prog. Brain Res."},{"key":"10.1016\/j.ymthe.2023.03.008_bib5","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.2174\/1570159X16666180419121247","article-title":"Glaucoma pathogenesis and neurotrophins: focus on the molecular and genetic basis for therapeutic prospects","volume":"16","author":"Chitranshi","year":"2018","journal-title":"Curr. Neuropharmacol."},{"key":"10.1016\/j.ymthe.2023.03.008_bib6","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1167\/iovs.04-0832","article-title":"Retinal ganglion cell apoptosis in glaucoma is related to intraocular pressure and IOP-induced effects on extracellular matrix","volume":"46","author":"Guo","year":"2005","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib7","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.tcm.2004.03.004","article-title":"Tissue-type plasminogen activator and neuroserpin: a well-balanced act in the nervous system?","volume":"14","author":"Yepes","year":"2004","journal-title":"Trends Cardiovasc. Med."},{"key":"10.1016\/j.ymthe.2023.03.008_bib8","doi-asserted-by":"crossref","first-page":"33062","DOI":"10.1074\/jbc.272.52.33062","article-title":"Neuroserpin, a brain-associated inhibitor of tissue plasminogen activator is localized primarily in neurons","volume":"272","author":"Hastings","year":"1997","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.ymthe.2023.03.008_bib9","doi-asserted-by":"crossref","first-page":"2312","DOI":"10.1074\/jbc.273.4.2312","article-title":"The axonally secreted serine proteinase inhibitor, neuroserpin, inhibits plasminogen activators and plasmin but not thrombin","volume":"273","author":"Osterwalder","year":"1998","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.ymthe.2023.03.008_bib10","doi-asserted-by":"crossref","first-page":"627896","DOI":"10.3389\/fnana.2021.627896","article-title":"Neuroserpin is strongly expressed in the developing and adult mouse neocortex but its absence does not perturb cortical lamination and synaptic proteome","volume":"15","author":"Kement","year":"2021","journal-title":"Front. Neuroanat."},{"key":"10.1016\/j.ymthe.2023.03.008_bib11","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1046\/j.1460-9568.2003.02628.x","article-title":"Expression of neuroserpin in the visual cortex of the mouse during the developmental critical period","volume":"17","author":"Wannier-Morino","year":"2003","journal-title":"Eur. J. Neurosci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib12","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1182\/blood.V96.2.569.014k35_569_576","article-title":"Neuroserpin reduces cerebral infarct volume and protects neurons from ischemia-induced apoptosis","volume":"96","author":"Yepes","year":"2000","journal-title":"Blood"},{"key":"10.1016\/j.ymthe.2023.03.008_bib13","doi-asserted-by":"crossref","first-page":"e63118","DOI":"10.1371\/journal.pone.0063118","article-title":"Deficiency in serine protease inhibitor neuroserpin exacerbates ischemic brain injury by increased postischemic inflammation","volume":"8","author":"Gelderblom","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.ymthe.2023.03.008_bib14","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1182\/blood.V96.2.569.014k35_569_576","article-title":"Neuroserpin reduces cerebral infarct volume and protects neurons from ischemia-induced apoptosis","volume":"96","author":"Yepes","year":"2000","journal-title":"Blood"},{"key":"10.1016\/j.ymthe.2023.03.008_bib15","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1038\/nm0298-228","article-title":"Tissue plasminogen activator (tPA) increases neuronal damage after focal cerebral ischemia in wild-type and tPA-deficient mice","volume":"4","author":"Wang","year":"1998","journal-title":"Nat. Med."},{"key":"10.1016\/j.ymthe.2023.03.008_bib16","doi-asserted-by":"crossref","first-page":"e0130440","DOI":"10.1371\/journal.pone.0130440","article-title":"Retina is protected by neuroserpin from ischemic\/reperfusion-induced injury independent of tissue-type plasminogen activator","volume":"10","author":"Gu","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.ymthe.2023.03.008_bib17","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/j.tcb.2005.09.005","article-title":"Cell toxicity and conformational disease","volume":"15","author":"Carrell","year":"2005","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.ymthe.2023.03.008_bib18","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1038\/nrg907","article-title":"Serpinopathies and the conformational dementias","volume":"3","author":"Lomas","year":"2002","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.ymthe.2023.03.008_bib19","doi-asserted-by":"crossref","first-page":"18202","DOI":"10.1074\/jbc.M109.010744","article-title":"Neuroserpin polymers activate NF-\u03baB by a calcium signaling pathway that is independent of the unfolded protein response","volume":"284","author":"Davies","year":"2009","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.ymthe.2023.03.008_bib20","doi-asserted-by":"crossref","first-page":"20835","DOI":"10.1074\/jbc.M110.200808","article-title":"The endoplasmic reticulum (ER)-associated degradation system regulates aggregation and degradation of mutant neuroserpin","volume":"286","author":"Ying","year":"2011","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.ymthe.2023.03.008_bib21","doi-asserted-by":"crossref","first-page":"22793","DOI":"10.1074\/jbc.M109.027102","article-title":"Endoplasmic reticulum-associated degradation (ERAD) and autophagy cooperate to degrade polymerogenic mutant serpins","volume":"284","author":"Kroeger","year":"2009","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.ymthe.2023.03.008_bib22","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1684\/epd.2016.0847","article-title":"Progressive myoclonus epilepsy associated with neuroserpin inclusion bodies (neuroserpinosis)","volume":"18","author":"Roussel","year":"2016","journal-title":"Epileptic Disord."},{"key":"10.1016\/j.ymthe.2023.03.008_bib23","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1093\/hmg\/ddn041","article-title":"The intracellular accumulation of polymeric neuroserpin explains the severity of the dementia FENIB","volume":"17","author":"Miranda","year":"2008","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.ymthe.2023.03.008_bib24","doi-asserted-by":"crossref","first-page":"28283","DOI":"10.1074\/jbc.M313166200","article-title":"Mutants of neuroserpin that cause dementia accumulate as polymers within the endoplasmic reticulum","volume":"279","author":"Miranda","year":"2004","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.ymthe.2023.03.008_bib25","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1038\/361453a0","article-title":"Tissue-plasminogen activator is induced as an immediate-early gene during seizure, kindling and long-term potentiation","volume":"361","author":"Qian","year":"1993","journal-title":"Nature"},{"key":"10.1016\/j.ymthe.2023.03.008_bib26","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.1523\/JNEUROSCI.22-06-02125.2002","article-title":"Localization and regulation of the tissue plasminogen activator-plasmin system in the hippocampus","volume":"22","author":"Salles","year":"2002","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib27","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1038\/nn998","article-title":"Tissue plasminogen activator in the amygdala is critical for stress-induced anxiety-like behavior","volume":"6","author":"Pawlak","year":"2003","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib28","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.neuron.2004.09.014","article-title":"Molecular mechanisms underlying emotional learning and memory in the lateral amygdala","volume":"44","author":"Rodrigues","year":"2004","journal-title":"Neuron"},{"key":"10.1016\/j.ymthe.2023.03.008_bib29","doi-asserted-by":"crossref","first-page":"1992","DOI":"10.1126\/science.270.5244.1992","article-title":"Tissue plasminogen activator induction in Purkinje neurons after cerebellar motor learning","volume":"270","author":"Seeds","year":"1995","journal-title":"Science"},{"key":"10.1016\/j.ymthe.2023.03.008_bib30","doi-asserted-by":"crossref","first-page":"14118","DOI":"10.1073\/pnas.96.24.14118","article-title":"Neuronal migration is retarded in mice lacking the tissue plasminogen activator gene","volume":"96","author":"Seeds","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ymthe.2023.03.008_bib31","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/S1044-7431(03)00077-0","article-title":"Impaired explorative behavior and neophobia in genetically modified mice lacking or overexpressing the extracellular serine protease inhibitor neuroserpin","volume":"23","author":"Madani","year":"2003","journal-title":"Mol. Cell. Neurosci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib32","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1007\/s00018-022-04185-6","article-title":"Neuroserpin, a crucial regulator for axogenesis, synaptic modelling and cell-cell interactions in the pathophysiology of neurological disease","volume":"79","author":"Godinez","year":"2022","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib33","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1006\/mcne.1999.0804","article-title":"Neuronal depolarization enhances the transcription of the neuronal serine protease inhibitor neuroserpin","volume":"14","author":"Berger","year":"1999","journal-title":"Mol. Cell. Neurosci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib34","doi-asserted-by":"crossref","first-page":"2202","DOI":"10.1093\/nar\/30.10.2202","article-title":"HuD binds to three AU-rich sequences in the 3'-UTR of neuroserpin mRNA and promotes the accumulation of neuroserpin mRNA and protein","volume":"30","author":"Cuadrado","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ymthe.2023.03.008_bib35","doi-asserted-by":"crossref","first-page":"3791","DOI":"10.1210\/en.2005-0108","article-title":"Expression of neuroserpin is linked to neuroendocrine cell activation","volume":"146","author":"de Groot","year":"2005","journal-title":"Endocrinology"},{"key":"10.1016\/j.ymthe.2023.03.008_bib36","doi-asserted-by":"crossref","first-page":"8412","DOI":"10.1038\/s41598-017-08688-2","article-title":"Glaucoma is associated with plasmin proteolytic activation mediated through oxidative inactivation of neuroserpin","volume":"7","author":"Gupta","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ymthe.2023.03.008_bib37","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.neuroscience.2022.01.020","article-title":"TrkB receptor agonist 7,8 dihydroxyflavone is protective against the inner retinal deficits induced by experimental glaucoma","volume":"490","author":"Gupta","year":"2022","journal-title":"Neuroscience"},{"key":"10.1016\/j.ymthe.2023.03.008_bib38","doi-asserted-by":"crossref","first-page":"6154","DOI":"10.7150\/thno.55472","article-title":"Inner retinal injury in experimental glaucoma is prevented upon AAV mediated Shp2 silencing in a caveolin dependent manner","volume":"11","author":"Abbasi","year":"2021","journal-title":"Theranostics"},{"key":"10.1016\/j.ymthe.2023.03.008_bib39","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.pep.2007.09.013","article-title":"Purification and biochemical characterization of ovine alpha-1-proteinase inhibitor: mechanistic adaptations and role of Phe350 and Met356","volume":"57","author":"Gupta","year":"2008","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.ymthe.2023.03.008_bib40","doi-asserted-by":"crossref","first-page":"6245","DOI":"10.1021\/bi025599p","article-title":"Relationship between protein structure and methionine oxidation in recombinant human alpha 1-antitrypsin","volume":"41","author":"Griffiths","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.ymthe.2023.03.008_bib41","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1101\/lm.045864.117","article-title":"The serine protease inhibitor neuroserpin is required for normal synaptic plasticity and regulates learning and social behavior","volume":"24","author":"Reumann","year":"2017","journal-title":"Learn. Mem."},{"key":"10.1016\/j.ymthe.2023.03.008_bib42","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1096\/fj.04-3403com","article-title":"Plasminogen activators promote excitotoxicity-induced retinal damage","volume":"19","author":"Mali","year":"2005","journal-title":"FASEB J."},{"key":"10.1016\/j.ymthe.2023.03.008_bib43","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.exer.2015.10.003","article-title":"Tissue and urokinase plasminogen activators instigate the degeneration of retinal ganglion cells in a mouse model of glaucoma","volume":"143","author":"Chintala","year":"2016","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.ymthe.2023.03.008_bib44","doi-asserted-by":"crossref","first-page":"2312","DOI":"10.1074\/jbc.273.4.2312","article-title":"The axonally secreted serine proteinase inhibitor, neuroserpin, inhibits plasminogen activators and plasmin but not thrombin","volume":"273","author":"Osterwalder","year":"1998","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.ymthe.2023.03.008_bib45","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.taap.2004.03.017","article-title":"Endogenous tissue type plasminogen activator facilitates NMDA-induced retinal damage","volume":"200","author":"Kumada","year":"2004","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.ymthe.2023.03.008_bib46","doi-asserted-by":"crossref","first-page":"3234","DOI":"10.1523\/JNEUROSCI.23-08-03234.2003","article-title":"Cell type-specific roles for tissue plasminogen activator released by neurons or microglia after excitotoxic injury","volume":"23","author":"Siao","year":"2003","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib47","first-page":"225","article-title":"Multilayered retinal microglial response to optic nerve transection in rats","volume":"11","author":"Garcia-Valenzuela","year":"2005","journal-title":"Mol. Vis."},{"key":"10.1016\/j.ymthe.2023.03.008_bib48","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1002\/cne.21279","article-title":"Rat retinal microglial cells under normal conditions, after optic nerve section, and after optic nerve section and intravitreal injection of trophic factors or macrophage inhibitory factor","volume":"501","author":"Sobrado-Calvo","year":"2007","journal-title":"J.\u00a0Comp. Neurol."},{"key":"10.1016\/j.ymthe.2023.03.008_bib49","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1001\/archopht.117.8.1050","article-title":"Microglia in the optic nerve head and the region of parapapillary chorioretinal atrophy in glaucoma","volume":"117","author":"Neufeld","year":"1999","journal-title":"Arch. Ophthalmol."},{"key":"10.1016\/j.ymthe.2023.03.008_bib50","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1002\/jnr.1104","article-title":"Activated microglia in the human glaucomatous optic nerve head","volume":"64","author":"Yuan","year":"2001","journal-title":"J.\u00a0Neurosci. Res."},{"key":"10.1016\/j.ymthe.2023.03.008_bib51","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1038\/nn1997","article-title":"Microglia: active sensor and versatile effector cells in the normal and pathologic brain","volume":"10","author":"Hanisch","year":"2007","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib52","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1189\/jlb.0207114","article-title":"Microglia activation in retinal degeneration","volume":"81","author":"Langmann","year":"2007","journal-title":"J.\u00a0Leukoc. Biol."},{"key":"10.1016\/j.ymthe.2023.03.008_bib53","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.tins.2008.09.006","article-title":"Tissue-type plasminogen activator in the ischemic brain: more than a thrombolytic","volume":"32","author":"Yepes","year":"2009","journal-title":"Trends Neurosci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib54","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1523\/JNEUROSCI.17-02-00543.1997","article-title":"An extracellular proteolytic cascade promotes neuronal degeneration in the mouse hippocampus","volume":"17","author":"Tsirka","year":"1997","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib55","doi-asserted-by":"crossref","first-page":"3352","DOI":"10.1523\/JNEUROSCI.22-09-03352.2002","article-title":"Tissue plasminogen activator mediates microglial activation via its finger domain through annexin II","volume":"22","author":"Siao","year":"2002","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib56","doi-asserted-by":"crossref","first-page":"7717","DOI":"10.1073\/pnas.102088899","article-title":"Permissive proteolytic activity for visual cortical plasticity","volume":"99","author":"Mataga","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ymthe.2023.03.008_bib57","doi-asserted-by":"crossref","first-page":"e91050","DOI":"10.1371\/journal.pone.0091050","article-title":"AAV-mediated overexpression of neuroserpin in the hippocampus decreases PSD-95 expression but does not affect hippocampal-dependent learning and memory","volume":"9","author":"Tsang","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.ymthe.2023.03.008_bib58","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1186\/s13041-014-0053-2","article-title":"Alterations of the synapse of the inner retinal layers after chronic intraocular pressure elevation in glaucoma animal model","volume":"7","author":"Park","year":"2014","journal-title":"Mol. Brain"},{"key":"10.1016\/j.ymthe.2023.03.008_bib59","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.jns.2005.03.015","article-title":"Neurofilament phosphoforms: surrogate markers for axonal injury, degeneration and loss","volume":"233","author":"Petzold","year":"2005","journal-title":"J.\u00a0Neurol. Sci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib60","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/0166-2236(91)90062-Y","article-title":"Neurofilament phosphorylation: a new look at regulation and function","volume":"14","author":"Nixon","year":"1991","journal-title":"Trends Neurosci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib61","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1002\/glia.20962","article-title":"Glaucomatous optic nerve injury involves early astrocyte reactivity and late oligodendrocyte loss","volume":"58","author":"Son","year":"2010","journal-title":"Glia"},{"key":"10.1016\/j.ymthe.2023.03.008_bib62","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1167\/iovs.02-0398","article-title":"Increase in dephosphorylation of the heavy neurofilament subunit in the monkey chronic glaucoma model","volume":"44","author":"Kashiwagi","year":"2003","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib63","doi-asserted-by":"crossref","first-page":"121","DOI":"10.3389\/fcell.2020.00121","article-title":"The role of autophagy in glaucomatous optic neuropathy","volume":"8","author":"Adornetto","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.ymthe.2023.03.008_bib64","doi-asserted-by":"crossref","first-page":"6064","DOI":"10.1073\/pnas.0909794107","article-title":"Mechanisms of acute axonal degeneration in the optic nerve in\u00a0vivo","volume":"107","author":"Kn\u00f6ferle","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ymthe.2023.03.008_bib65","doi-asserted-by":"crossref","first-page":"e22514","DOI":"10.1371\/journal.pone.0022514","article-title":"Activation of autophagy in a rat model of retinal ischemia following high intraocular pressure","volume":"6","author":"Piras","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.ymthe.2023.03.008_bib66","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1007\/978-1-4614-3209-8_85","article-title":"Autophagy induction does not protect retina against apoptosis in ischemia\/reperfusion model","volume":"801","author":"Produit-Zengaffinen","year":"2014","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.ymthe.2023.03.008_bib67","doi-asserted-by":"crossref","first-page":"476","DOI":"10.3892\/etm.2014.2084","article-title":"Activation of autophagy and paraptosis in retinal ganglion cells after retinal ischemia and reperfusion injury in rats","volume":"9","author":"Wei","year":"2015","journal-title":"Exp. Ther. Med."},{"key":"10.1016\/j.ymthe.2023.03.008_bib68","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1038\/s41420-018-0077-y","article-title":"Contribution of autophagy to ocular hypertension and neurodegeneration in the DBA\/2J spontaneous glaucoma mouse model","volume":"4","author":"Hirt","year":"2018","journal-title":"Cell Death Discov."},{"key":"10.1016\/j.ymthe.2023.03.008_bib69","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.ymthe.2020.11.029","article-title":"Clinical perspective: treating RPE65-associated retinal dystrophy","volume":"29","author":"Maguire","year":"2021","journal-title":"Mol. Ther."},{"key":"10.1016\/j.ymthe.2023.03.008_bib70","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1016\/j.drudis.2019.01.019","article-title":"Luxturna: FDA documents reveal the value of a costly gene therapy","volume":"24","author":"Darrow","year":"2019","journal-title":"Drug Discov. Today"},{"key":"10.1016\/j.ymthe.2023.03.008_bib71","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s11095-018-2554-7","article-title":"Pharmaceutical development of AAV-based gene therapy products for the eye","volume":"36","author":"Rodrigues","year":"2018","journal-title":"Pharm. Res."},{"key":"10.1016\/j.ymthe.2023.03.008_bib72","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/j.ymthe.2018.09.019","article-title":"Loss of Shp2 rescues BDNF\/TrkB signaling and contributes to improved retinal ganglion cell neuroprotection","volume":"27","author":"Chitranshi","year":"2019","journal-title":"Mol. Ther."},{"key":"10.1016\/j.ymthe.2023.03.008_bib73","doi-asserted-by":"crossref","first-page":"8984","DOI":"10.1523\/JNEUROSCI.17-23-08984.1997","article-title":"Expression of neuroserpin, an inhibitor of tissue plasminogen activator, in the developing and adult nervous system of the mouse","volume":"17","author":"Krueger","year":"1997","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib74","doi-asserted-by":"crossref","first-page":"3931","DOI":"10.1016\/S0021-9258(19)85615-6","article-title":"Kinetics of association of serine proteinases with native and oxidized alpha-1-proteinase inhibitor and alpha-1-antichymotrypsin","volume":"255","author":"Beatty","year":"1980","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.ymthe.2023.03.008_bib75","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.neuroscience.2017.09.028","article-title":"PTPN11 induces endoplasmic stress and apoptosis in SH-SY5Y cells","volume":"364","author":"Chitranshi","year":"2017","journal-title":"Neuroscience"},{"key":"10.1016\/j.ymthe.2023.03.008_bib76","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.2353\/ajpath.2007.060910","article-title":"Accumulation of mutant neuroserpin precedes development of clinical symptoms in familial encephalopathy with neuroserpin inclusion bodies","volume":"170","author":"Galliciotti","year":"2007","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.ymthe.2023.03.008_bib77","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.expneurol.2019.01.015","article-title":"Retinoid x receptor modulation protects against ER stress response and rescues glaucoma phenotypes in adult mice","volume":"314","author":"Dheer","year":"2019","journal-title":"Exp. Neurol."},{"key":"10.1016\/j.ymthe.2023.03.008_bib78","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1017\/S0952523806231080","article-title":"Long-term ERG analysis in the partially light-damaged mouse retina reveals regressive and compensatory changes","volume":"23","author":"Richards","year":"2006","journal-title":"Vis. Neurosci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib79","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1016\/j.bbadis.2014.05.026","article-title":"BDNF impairment is associated with age-related changes in the inner retina and exacerbates experimental glaucoma","volume":"1842","author":"Gupta","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ymthe.2023.03.008_bib80","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/s12551-011-0054-7","article-title":"Optical coherence tomography: fundamental principles, instrumental designs and biomedical applications","volume":"3","author":"Popescu","year":"2011","journal-title":"Biophys. Rev."},{"key":"10.1016\/j.ymthe.2023.03.008_bib81","doi-asserted-by":"crossref","first-page":"3060","DOI":"10.1167\/iovs.13-13262","article-title":"FTY720 protects retinal ganglion cells in experimental glaucoma","volume":"55","author":"You","year":"2014","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib82","doi-asserted-by":"crossref","first-page":"4440","DOI":"10.1167\/iovs.13-11899","article-title":"Noninvasive assessments of optic nerve neurodegeneration in transgenic mice with isolated optic neuritis","volume":"54","author":"Talla","year":"2013","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.ymthe.2023.03.008_bib83","first-page":"58031","article-title":"Toluidine blue staining of resin-embedded sections for evaluation of peripheral nerve morphology","author":"Ghnenis","year":"2018","journal-title":"J.\u00a0Vis. Exp."},{"key":"10.1016\/j.ymthe.2023.03.008_bib84","doi-asserted-by":"crossref","first-page":"3975","DOI":"10.1128\/MCB.05659-11","article-title":"Phosphorylated Grb14 is an endogenous inhibitor of retinal protein tyrosine phosphatase 1B, and light-dependent activation of Src phosphorylates Grb14","volume":"31","author":"Basavarajappa","year":"2011","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.ymthe.2023.03.008_bib85","doi-asserted-by":"crossref","first-page":"E1363","DOI":"10.1152\/ajpendo.00199.2012","article-title":"Insulin receptor regulates photoreceptor CNG channel activity","volume":"303","author":"Gupta","year":"2012","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"10.1016\/j.ymthe.2023.03.008_bib86","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1038\/embor.2010.142","article-title":"Growth factor receptor-bound protein 14: a new modulator of photoreceptor-specific cyclic-nucleotide-gated channel","volume":"11","author":"Gupta","year":"2010","journal-title":"EMBO Rep."},{"key":"10.1016\/j.ymthe.2023.03.008_bib87","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1016\/j.biochi.2008.01.004","article-title":"Alpha-1-proteinase inhibitor is a heparin binding serpin: molecular interactions with the Lys rich cluster of helix-F domain","volume":"90","author":"Gupta","year":"2008","journal-title":"Biochimie"},{"key":"10.1016\/j.ymthe.2023.03.008_bib88","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.bbrc.2014.10.087","article-title":"Brain derived neurotrophic factor is involved in the regulation of glycogen synthase kinase 3beta (GSK3beta) signalling","volume":"454","author":"Gupta","year":"2014","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.ymthe.2023.03.008_bib89","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.neulet.2016.04.059","article-title":"Amyloid beta accumulation and inner retinal degenerative changes in Alzheimer's disease transgenic mouse","volume":"623","author":"Gupta","year":"2016","journal-title":"Neurosci. Lett."}],"container-title":["Molecular Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525001623001296?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525001623001296?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T18:09:29Z","timestamp":1761502169000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1525001623001296"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7]]},"references-count":89,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["S1525001623001296"],"URL":"https:\/\/doi.org\/10.1016\/j.ymthe.2023.03.008","relation":{},"ISSN":["1525-0016"],"issn-type":[{"value":"1525-0016","type":"print"}],"subject":[],"published":{"date-parts":[[2023,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Neuroserpin gene therapy inhibits retinal ganglion cell apoptosis and promotes functional preservation in glaucoma","name":"articletitle","label":"Article Title"},{"value":"Molecular Therapy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymthe.2023.03.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Author(s).","name":"copyright","label":"Copyright"}]}}