{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:19:00Z","timestamp":1790198340397,"version":"4.1.0"},"reference-count":58,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100017610","name":"Shenzhen Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["JCYJ20230807095202004"],"award-info":[{"award-number":["JCYJ20230807095202004"]}],"id":[{"id":"10.13039\/501100017610","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017610","name":"Shenzhen Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["JCYJ20240813120016022"],"award-info":[{"award-number":["JCYJ20240813120016022"]}],"id":[{"id":"10.13039\/501100017610","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82371727"],"award-info":[{"award-number":["82371727"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470899"],"award-info":[{"award-number":["32470899"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81801517"],"award-info":[{"award-number":["81801517"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470903"],"award-info":[{"award-number":["32470903"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009659","name":"Guangzhou Medical University","doi-asserted-by":"publisher","award":["GMUCR2025-02022"],"award-info":[{"award-number":["GMUCR2025-02022"]}],"id":[{"id":"10.13039\/100009659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007935","name":"Hainan Medical University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007935","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515010618"],"award-info":[{"award-number":["2023A1515010618"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2022A1515220214"],"award-info":[{"award-number":["2022A1515220214"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFE0138700"],"award-info":[{"award-number":["2022YFE0138700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC2705605"],"award-info":[{"award-number":["2023YFC2705605"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2703202"],"award-info":[{"award-number":["2022YFC2703202"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2702603"],"award-info":[{"award-number":["2022YFC2702603"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018557","name":"Science and Technology Project of Nantong City","doi-asserted-by":"publisher","award":["MS12022027"],"award-info":[{"award-number":["MS12022027"]}],"id":[{"id":"10.13039\/501100018557","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004000","name":"Guangzhou Municipal Science and Technology Program key projects","doi-asserted-by":"publisher","award":["202206010100"],"award-info":[{"award-number":["202206010100"]}],"id":[{"id":"10.13039\/501100004000","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20221376"],"award-info":[{"award-number":["BK20221376"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Therapy"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.ymthe.2025.08.009","type":"journal-article","created":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T15:51:59Z","timestamp":1754668319000},"page":"5883-5900","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"title":["METTL5 deficiency induces oligoasthenoteratozoospermia via impaired 18S rRNA m6A methylation in humans and mice"],"prefix":"10.1016","volume":"33","author":[{"given":"Mengya","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueguang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Qiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gelin","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoye","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huixia","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liandong","family":"Zuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunfang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue-Qiu","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuibin","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenming","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8319-8101","authenticated-orcid":false,"given":"Hao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ymthe.2025.08.009_bib1","doi-asserted-by":"crossref","first-page":"14","DOI":"10.4161\/spmg.1.1.14525","article-title":"Spermiation: The process of sperm release","volume":"1","author":"O'Donnell","year":"2011","journal-title":"Spermatogenesis"},{"key":"10.1016\/j.ymthe.2025.08.009_bib2","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1530\/rep.0.1210347","article-title":"Proliferation and differentiation of spermatogonial stem cells","volume":"121","author":"de Rooij","year":"2001","journal-title":"Reproduction"},{"key":"10.1016\/j.ymthe.2025.08.009_bib3","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1152\/physrev.1972.52.1.198","article-title":"Kinetics of spermatogenesis in mammals: seminiferous epithelium cycle and spermatogonial renewal","volume":"52","author":"Clermont","year":"1972","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.ymthe.2025.08.009_bib4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1152\/physrev.00013.2015","article-title":"Spermatogenesis: The Commitment to Meiosis","volume":"96","author":"Griswold","year":"2016","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.ymthe.2025.08.009_bib5","doi-asserted-by":"crossref","first-page":"4893","DOI":"10.1007\/s00018-021-03823-9","article-title":"Epigenetic regulations in mammalian spermatogenesis: RNA-m(6)A modification and beyond","volume":"78","author":"Gui","year":"2021","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.ymthe.2025.08.009_bib6","article-title":"The Latest Research Progress of m(6)A Modification and Its Writers, Erasers, Readers in Infertility: A Review","volume":"9","author":"Liu","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.ymthe.2025.08.009_bib7","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.bbagrm.2018.10.016","article-title":"m(6)A mRNA modification regulates mammalian spermatogenesis","volume":"1862","author":"Lin","year":"2019","journal-title":"Biochim. Biophys. Acta Gene Regul. Mech."},{"key":"10.1016\/j.ymthe.2025.08.009_bib8","doi-asserted-by":"crossref","DOI":"10.1093\/molehr\/gaab025","article-title":"Epigenetic regulation of mRNA N6-methyladenosine modifications in mammalian gametogenesis","volume":"27","author":"Fang","year":"2021","journal-title":"Mol. Hum. Reprod."},{"key":"10.1016\/j.ymthe.2025.08.009_bib9","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2022.819044","article-title":"Genetic Regulation of N6-Methyladenosine-RNA in Mammalian Gametogenesis and Embryonic Development","volume":"10","author":"Chang","year":"2022","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.ymthe.2025.08.009_bib10","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1016\/j.molcel.2019.04.025","article-title":"Where, When, and How: Context-Dependent Functions of RNA Methylation Writers, Readers, and Erasers","volume":"74","author":"Shi","year":"2019","journal-title":"Mol. Cell"},{"key":"10.1016\/j.ymthe.2025.08.009_bib11","doi-asserted-by":"crossref","first-page":"7719","DOI":"10.1093\/nar\/gkz619","article-title":"The human 18S rRNA m6A methyltransferase METTL5 is stabilized by TRMT112","volume":"47","author":"van Tran","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ymthe.2025.08.009_bib12","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1038\/s41589-018-0184-3","article-title":"N(6-)Methyladenosine methyltransferase ZCCHC4 mediates ribosomal RNA methylation","volume":"15","author":"Ma","year":"2019","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.ymthe.2025.08.009_bib13","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1016\/j.ajhg.2019.09.007","article-title":"Bi-allelic Variants in METTL5 Cause Autosomal-Recessive Intellectual Disability and Microcephaly","volume":"105","author":"Richard","year":"2019","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.ymthe.2025.08.009_bib14","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.gendis.2020.07.004","article-title":"Mettl5 mediated 18S rRNA N6-methyladenosine (m(6)A) modification controls stem cell fate determination and neural function","volume":"9","author":"Wang","year":"2022","journal-title":"Genes Dis."},{"key":"10.1016\/j.ymthe.2025.08.009_bib15","doi-asserted-by":"crossref","DOI":"10.15252\/embr.201949863","article-title":"The 18S rRNA m(6) A methyltransferase METTL5 promotes mouse embryonic stem cell differentiation","volume":"21","author":"Xing","year":"2020","journal-title":"EMBO Rep."},{"key":"10.1016\/j.ymthe.2025.08.009_bib16","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1038\/s42255-022-00622-9","article-title":"N(6)-methyladenosine (m(6)A) in 18S rRNA promotes fatty acid metabolism and oncogenic transformation","volume":"4","author":"Peng","year":"2022","journal-title":"Nat. Metab."},{"key":"10.1016\/j.ymthe.2025.08.009_bib17","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.chembiol.2023.01.006","article-title":"N(6)-methyladenosine modification in 18S rRNA promotes tumorigenesis and chemoresistance via HSF4b\/HSP90B1\/mutant p53 axis","volume":"30","author":"Chen","year":"2023","journal-title":"Cell Chem. Biol."},{"key":"10.1016\/j.ymthe.2025.08.009_bib18","article-title":"Ribosome 18S m(6)A methyltransferase METTL5 promotes pancreatic cancer progression by modulating c-Myc translation","volume":"60","author":"Huang","year":"2022","journal-title":"Int. J. Oncol."},{"key":"10.1016\/j.ymthe.2025.08.009_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2020.108544","article-title":"Ribosome 18S m(6)A Methyltransferase METTL5 Promotes Translation Initiation and Breast Cancer Cell Growth","volume":"33","author":"Rong","year":"2020","journal-title":"Cell Rep."},{"key":"10.1016\/j.ymthe.2025.08.009_bib20","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1101\/gad.333369.119","article-title":"The rRNA m(6)A methyltransferase METTL5 is involved in pluripotency and developmental programs","volume":"34","author":"Ignatova","year":"2020","journal-title":"Genes Dev."},{"key":"10.1016\/j.ymthe.2025.08.009_bib21","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.1093\/biolre\/ioz013","article-title":"A high-throughput method for unbiased quantitation and categorization of nuclear morphologydagger","volume":"100","author":"Skinner","year":"2019","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.ymthe.2025.08.009_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2022.101590","article-title":"The METTL5-TRMT112 N(6)-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation","volume":"298","author":"Sepich-Poore","year":"2022","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ymthe.2025.08.009_bib23","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1038\/nrg3645","article-title":"Ribosome profiling: new views of translation, from single codons to genome scale","volume":"15","author":"Ingolia","year":"2014","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.ymthe.2025.08.009_bib24","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-023-37252-y","article-title":"The m6A reader PRRC2A is essential for meiosis I completion during spermatogenesis","volume":"14","author":"Tan","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ymthe.2025.08.009_bib25","doi-asserted-by":"crossref","first-page":"2179","DOI":"10.1016\/j.celrep.2013.05.031","article-title":"Cellular source and mechanisms of high transcriptome complexity in the mammalian testis","volume":"3","author":"Soumillon","year":"2013","journal-title":"Cell Rep."},{"key":"10.1016\/j.ymthe.2025.08.009_bib26","doi-asserted-by":"crossref","DOI":"10.1093\/molehr\/gaab020","article-title":"RNA N6-methyladenosine modification, spermatogenesis, and human male infertility","volume":"27","author":"Cai","year":"2021","journal-title":"Mol. Hum. Reprod."},{"key":"10.1016\/j.ymthe.2025.08.009_bib27","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1002\/cac2.12403","article-title":"METTL5 stabilizes c-Myc by facilitating USP5 translation to reprogram glucose metabolism and promote hepatocellular carcinoma progression","volume":"43","author":"Xia","year":"2023","journal-title":"Cancer Commun."},{"key":"10.1016\/j.ymthe.2025.08.009_bib28","doi-asserted-by":"crossref","DOI":"10.1126\/science.abj6647","article-title":"LLPS of FXR1 drives spermiogenesis by activating translation of stored mRNAs","volume":"377","author":"Kang","year":"2022","journal-title":"Science"},{"key":"10.1016\/j.ymthe.2025.08.009_bib29","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1038\/cr.2017.117","article-title":"Mettl3-\/Mettl14-mediated mRNA N(6)-methyladenosine modulates murine spermatogenesis","volume":"27","author":"Lin","year":"2017","journal-title":"Cell Res."},{"key":"10.1016\/j.ymthe.2025.08.009_bib30","doi-asserted-by":"crossref","DOI":"10.1111\/cpr.13164","article-title":"m(6) A reader protein YTHDF2 regulates spermatogenesis by timely clearance of phase-specific transcripts","volume":"55","author":"Qi","year":"2022","journal-title":"Cell Prolif."},{"key":"10.1016\/j.ymthe.2025.08.009_bib31","doi-asserted-by":"crossref","first-page":"7712","DOI":"10.1073\/pnas.0510999103","article-title":"Expression profiling reveals meiotic male germ cell mRNAs that are translationally up- and down-regulated","volume":"103","author":"Iguchi","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ymthe.2025.08.009_bib32","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1095\/biolreprod.109.079699","article-title":"Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice","volume":"82","author":"Danshina","year":"2010","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.ymthe.2025.08.009_bib33","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1080\/15476286.2022.2079890","article-title":"RNA folding and functions of RNA helicases in ribosome biogenesis","volume":"19","author":"Mitterer","year":"2022","journal-title":"RNA Biol."},{"key":"10.1016\/j.ymthe.2025.08.009_bib34","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1136\/jmedgenet-2021-108406","article-title":"FSIP2 plays a role in the acrosome development during spermiogenesis","volume":"60","author":"Zheng","year":"2023","journal-title":"J. Med. Genet."},{"key":"10.1016\/j.ymthe.2025.08.009_bib35","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jgg.2018.09.006","article-title":"Homozygous loss-of-function mutations in FSIP2 cause male infertility with asthenoteratospermia","volume":"46","author":"Liu","year":"2019","journal-title":"J. Genet. Genomics"},{"key":"10.1016\/j.ymthe.2025.08.009_bib36","doi-asserted-by":"crossref","DOI":"10.1242\/dev.199216","article-title":"Hypomorphic and hypermorphic mouse models of Fsip2 indicate its dosage-dependent roles in sperm tail and acrosome formation","volume":"148","author":"Fang","year":"2021","journal-title":"Development"},{"key":"10.1016\/j.ymthe.2025.08.009_bib37","doi-asserted-by":"crossref","first-page":"2697","DOI":"10.1007\/s43032-022-00965-4","article-title":"Novel Compound Heterozygous Mutation in FSIP2 Causes Multiple Morphological Abnormalities of the Sperm Flagella (MMAF) and Male Infertility","volume":"29","author":"Hou","year":"2022","journal-title":"Reprod. Sci."},{"key":"10.1016\/j.ymthe.2025.08.009_bib38","doi-asserted-by":"crossref","first-page":"393","DOI":"10.7150\/ijbs.76051","article-title":"Novel FSIP2 Variants Induce Super-Length Mitochondrial Sheath and Asthenoteratozoospermia in Humans","volume":"19","author":"Lv","year":"2023","journal-title":"Int. J. Biol. Sci."},{"key":"10.1016\/j.ymthe.2025.08.009_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.gene.2021.145536","article-title":"Novel mutations in FSIP2 lead to multiple morphological abnormalities of the sperm flagella and poor ICSI prognosis","volume":"781","author":"Liu","year":"2021","journal-title":"Gene"},{"key":"10.1016\/j.ymthe.2025.08.009_bib40","doi-asserted-by":"crossref","DOI":"10.1186\/1471-213X-10-67","article-title":"Gene trap mutation of murine outer dense fiber protein-2 gene can result in sperm tail abnormalities in mice with high percentage chimaerism","volume":"10","author":"Tarnasky","year":"2010","journal-title":"BMC Dev. Biol."},{"key":"10.1016\/j.ymthe.2025.08.009_bib41","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1262\/jrd.2018-136","article-title":"Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis","volume":"65","author":"Shimada","year":"2019","journal-title":"J. Reprod. Dev."},{"key":"10.1016\/j.ymthe.2025.08.009_bib42","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1093\/hmg\/ddab234","article-title":"Loss-of-function missense variant of AKAP4 induced male infertility through reduced interaction with QRICH2 during sperm flagella development","volume":"31","author":"Zhang","year":"2021","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.ymthe.2025.08.009_bib43","doi-asserted-by":"crossref","first-page":"2790","DOI":"10.1093\/humrep\/dei126","article-title":"Gene deletions in an infertile man with sperm fibrous sheath dysplasia","volume":"20","author":"Baccetti","year":"2005","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.ymthe.2025.08.009_bib44","doi-asserted-by":"crossref","DOI":"10.1186\/s13578-022-00924-1","article-title":"Defb19 regulates the migration of germ cell and is involved in male fertility","volume":"12","author":"Jin","year":"2022","journal-title":"Cell Biosci."},{"key":"10.1016\/j.ymthe.2025.08.009_bib45","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.50209","article-title":"HIPK4 is essential for murine spermiogenesis","volume":"9","author":"Crapster","year":"2020","journal-title":"Elife"},{"key":"10.1016\/j.ymthe.2025.08.009_bib46","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-022-34990-3","article-title":"Targeting APLN\/APJ restores blood-testis barrier and improves spermatogenesis in murine and human diabetic models","volume":"13","author":"Song","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ymthe.2025.08.009_bib47","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0042882","article-title":"Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue","volume":"7","author":"Morlan","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.ymthe.2025.08.009_bib48","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1089\/cmb.2017.0096","article-title":"cutPrimers: A New Tool for Accurate Cutting of Primers from Reads of Targeted Next Generation Sequencing","volume":"24","author":"Kechin","year":"2017","journal-title":"J. Comput. Biol."},{"key":"10.1016\/j.ymthe.2025.08.009_bib49","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ymthe.2025.08.009_bib50","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ymthe.2025.08.009_bib51","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ymthe.2025.08.009_bib52","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","article-title":"featureCounts: an efficient general purpose program for assigning sequence reads to genomic features","volume":"30","author":"Liao","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ymthe.2025.08.009_bib53","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gky179","article-title":"De novo annotation and characterization of the translatome with ribosome profiling data","volume":"46","author":"Xiao","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ymthe.2025.08.009_bib54","doi-asserted-by":"crossref","DOI":"10.1186\/s12859-020-03670-8","article-title":"RiboMiner: a toolset for mining multi-dimensional features of the translatome with ribosome profiling data","volume":"21","author":"Li","year":"2020","journal-title":"BMC Bioinformatics"},{"key":"10.1016\/j.ymthe.2025.08.009_bib55","doi-asserted-by":"crossref","first-page":"15995","DOI":"10.1002\/anie.201807942","article-title":"An Elongation- and Ligation-Based qPCR Amplification Method for the Radiolabeling-Free Detection of Locus-Specific N(6) -Methyladenosine Modification","volume":"57","author":"Xiao","year":"2018","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.ymthe.2025.08.009_bib56","doi-asserted-by":"crossref","DOI":"10.1186\/s12915-023-01537-x","article-title":"Hypoxia induces alterations in tRNA modifications involved in translational control","volume":"21","author":"Guo","year":"2023","journal-title":"BMC Biol."},{"key":"10.1016\/j.ymthe.2025.08.009_bib57","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2022.101398","article-title":"Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse","volume":"3","author":"Zou","year":"2022","journal-title":"STAR Protoc."},{"key":"10.1016\/j.ymthe.2025.08.009_bib58","doi-asserted-by":"crossref","first-page":"3339","DOI":"10.1016\/j.molcel.2021.07.003","article-title":"N(7)-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression","volume":"81","author":"Dai","year":"2021","journal-title":"Mol. Cell"}],"container-title":["Molecular Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525001625006392?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525001625006392?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T08:23:32Z","timestamp":1779524612000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1525001625006392"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":58,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["S1525001625006392"],"URL":"https:\/\/doi.org\/10.1016\/j.ymthe.2025.08.009","relation":{},"ISSN":["1525-0016"],"issn-type":[{"value":"1525-0016","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"METTL5 deficiency induces oligoasthenoteratozoospermia via impaired 18S rRNA m6A methylation in humans and mice","name":"articletitle","label":"Article Title"},{"value":"Molecular Therapy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ymthe.2025.08.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The American Society of Gene and Cell Therapy. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}