{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T18:34:30Z","timestamp":1789929270553,"version":"4.0.1"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Cefic LRI"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Regulatory Toxicology and Pharmacology"],"published-print":{"date-parts":[[2016,10]]},"DOI":"10.1016\/j.yrtph.2016.07.013","type":"journal-article","created":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T03:40:07Z","timestamp":1469590807000},"page":"255-259","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":25,"special_numbering":"C","title":["Chemical applicability domain of the Local Lymph Node Assay (LLNA) for skin sensitisation potency. Part 2. The biological variability of the murine Local Lymph Node Assay (LLNA) for skin sensitisation"],"prefix":"10.1016","volume":"80","author":[{"given":"David W.","family":"Roberts","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne Marie","family":"Api","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aynur O.","family":"Aptula","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.yrtph.2016.07.013_bib1","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1111\/j.1600-0536.2007.01141.x","article-title":"The local lymph node assay and the assessment of relative potency: status of validation","volume":"57","author":"Basketter","year":"2007","journal-title":"Contact Dermat."},{"key":"10.1016\/j.yrtph.2016.07.013_bib2","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1111\/j.1600-0536.2008.01444.x","article-title":"Nothing is perfect, not even the local lymph node assay. A commentary and the implications for REACH","volume":"60","author":"Basketter","year":"2009","journal-title":"Contact Dermat."},{"key":"10.1016\/j.yrtph.2016.07.013_bib3","series-title":"EURL ECVAM Recommendation on the Human Cell Line Activation Test (H-clat) for Skin Sensitisation Testing","author":"EURL-ECVAM","year":"2015"},{"key":"10.1016\/j.yrtph.2016.07.013_bib4","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1111\/j.0105-1873.2004.00290.x","article-title":"A\u00a0chemical dataset for evaluation of alternative approaches to skin-sensitization testing","volume":"50","author":"Gerberick","year":"2004","journal-title":"Contact Dermat."},{"key":"10.1016\/j.yrtph.2016.07.013_bib5","first-page":"157","article-title":"Compilation of historical local lymph node data for evaluation of skin sensitization alternative methods","volume":"16","author":"Gerberick","year":"2005","journal-title":"Dermatitis"},{"key":"10.1016\/j.yrtph.2016.07.013_bib6","series-title":"Substituent Constants for Correlation Analysis in Chemistry and Biology","author":"Hansch","year":"1979"},{"issue":"11","key":"10.1016\/j.yrtph.2016.07.013_bib7","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1002\/jat.3105","article-title":"Evaluation of combinations of in\u00a0vitro sensitization test descriptors for the artificial neural network-based risk assessment model of skin sensitization","volume":"35","author":"Hirota","year":"2015","journal-title":"J.\u00a0Appl. Toxicol."},{"issue":"4","key":"10.1016\/j.yrtph.2016.07.013_bib8","first-page":"379","article-title":"LLNA variability: an essential ingredient for a comprehensive assessment of non-animal skin sensitization test methods and strategies","volume":"32","author":"Hoffmann","year":"2015","journal-title":"ALTEX"},{"key":"10.1016\/j.yrtph.2016.07.013_bib9","series-title":"Physical Organic Chemistry","author":"Isaacs","year":"1995"},{"key":"10.1016\/j.yrtph.2016.07.013_bib10","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1007\/s00204-015-1634-2","article-title":"Bayesian integrated testing strategy (ITS) for skin sensitization potency assessment: a decision support system for quantitative weight of evidence and adaptive testing strategy","volume":"89","author":"Jaworska","year":"2015","journal-title":"Arch. Toxicol."},{"issue":"2","key":"10.1016\/j.yrtph.2016.07.013_bib11","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1080\/15569520801904655","article-title":"The impact of vehicle on the relative potency of skin sensitising chemicals in the local lymph node assay","volume":"27","author":"Jowsey","year":"2008","journal-title":"Cutan. Ocul. Toxicol."},{"issue":"1","key":"10.1016\/j.yrtph.2016.07.013_bib12","doi-asserted-by":"crossref","first-page":"8","DOI":"10.2310\/6620.2009.09038","article-title":"Local Lymph Node data for the evaluation of skin sensitization alternatives: a second compilation","volume":"21","author":"Kern","year":"2010","journal-title":"Dermatitis"},{"key":"10.1016\/j.yrtph.2016.07.013_bib13","series-title":"US Patent US5728881 a","author":"Kwiatkowski","year":"1998"},{"issue":"4","key":"10.1016\/j.yrtph.2016.07.013_bib14","doi-asserted-by":"crossref","first-page":"473","DOI":"10.14573\/altex.2013.4.473","article-title":"From pathways to people: applying the adverse outcome pathway (AOP) for skin sensitization to risk assessment","volume":"30","author":"MacKay","year":"2013","journal-title":"ALTEX"},{"issue":"1","key":"10.1016\/j.yrtph.2016.07.013_bib15","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.tiv.2013.10.013","article-title":"Applying the skin sensitisation adverse outcome pathway (AOP) to quantitative risk assessment","volume":"28","author":"Maxwell","year":"2014","journal-title":"Toxicol. vitro"},{"key":"10.1016\/j.yrtph.2016.07.013_bib16","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1093\/toxsci\/kfu229","article-title":"Predicting skin sensitizer potency based on in\u00a0vitro data from KeratinoSens and kinetic peptide binding: global versus domain-based assessment","volume":"143","author":"Natsch","year":"2015","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.yrtph.2016.07.013_bib17","series-title":"OECD Guideline for the Testing of Chemicals 406: Skin Sensitisation","author":"OECD","year":"1992"},{"key":"10.1016\/j.yrtph.2016.07.013_bib18","series-title":"OECD Guideline for the Testing of Chemicals 429: Skin Sensitisation: Local Lymph Node Assay","author":"OECD","year":"2010"},{"key":"10.1016\/j.yrtph.2016.07.013_bib19","series-title":"OECD TG 442C: in Chemico Skin Sensitization: Direct Peptide Reactivity Assay (DPRA)","author":"OECD","year":"2015"},{"key":"10.1016\/j.yrtph.2016.07.013_bib20","series-title":"OECD TG 442D: in\u00a0Vitro Skin Sensitization: ARE-nrf2 Luciferase Test Methods","author":"OECD","year":"2015"},{"issue":"3","key":"10.1016\/j.yrtph.2016.07.013_bib21","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.yrtph.2014.06.001","article-title":"Towards AOP application - implementation of an integrated approach to testing and assessment (IATA) into a pipeline tool for skin sensitization","volume":"69","author":"Patlewicz","year":"2014","journal-title":"Reg. Toxicol. Pharmacol."},{"issue":"1","key":"10.1016\/j.yrtph.2016.07.013_bib22","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.tiv.2014.10.018","article-title":"Systematic evaluation of non-animal test methods for skin sensitisation safety assessment","volume":"29","author":"Reisinger","year":"2015","journal-title":"Toxicol. vitro"},{"issue":"9","key":"10.1016\/j.yrtph.2016.07.013_bib23","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1021\/tx060102o","article-title":"Mechanistic applicability domains for non-animal based prediction of toxicological endpoints. QSAR analysis of the Schiff Base applicability domain for skin sensitization","volume":"19","author":"Roberts","year":"2006","journal-title":"Chem. Res. Tox"},{"key":"10.1016\/j.yrtph.2016.07.013_bib24","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1021\/tx060121y","article-title":"Electrophilic chemistry related to skin sensitization. Reaction mechanistic applicability domain classification for a published data set of 106 chemicals tested in the Mouse Local Lymph Node Assay","volume":"20","author":"Roberts","year":"2007","journal-title":"Chem. Res. Tox."},{"key":"10.1016\/j.yrtph.2016.07.013_bib25","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1021\/tx800431x","article-title":"High throughput kinetic profiling approach for covalent binding to peptides: application to skin sensitization potency of Michael acceptor electrophiles","volume":"22","author":"Roberts","year":"2009","journal-title":"Chem. Res. Tox."},{"key":"10.1016\/j.yrtph.2016.07.013_bib26","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1021\/tx400355n","article-title":"Electrophilic reactivity and skin sensitization potency of SNAr electrophiles","volume":"27","author":"Roberts","year":"2014","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/j.yrtph.2016.07.013_bib27","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.yrtph.2015.01.009","article-title":"Estimating skin sensitization potency from a single dose LLNA","volume":"71","author":"Roberts","year":"2015","journal-title":"Reg. Tox. Pharmacol."},{"key":"10.1016\/j.yrtph.2016.07.013_bib28","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1080\/15569520701212258","article-title":"Extrapolating local lymph node assay EC3 values to estimate relative sensitizing potency","volume":"26","author":"Ryan","year":"2007","journal-title":"Cutan. Ocul. Tox"},{"issue":"2","key":"10.1016\/j.yrtph.2016.07.013_bib29","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/1710-1492-4-2-59","article-title":"Occupational contact dermatitis","volume":"4","author":"Sasseville","year":"2008","journal-title":"Allergy Asthma Clin. Immunol."},{"issue":"11","key":"10.1016\/j.yrtph.2016.07.013_bib30","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1002\/jat.3127","article-title":"Test battery with the human cell line activation test, direct peptide reactivity assay and DEREK based on a 139 chemical data set for predicting skin sensitizing potential and potency of chemicals","volume":"35","author":"Takenouchi","year":"2015","journal-title":"J.\u00a0Appl. Toxicol."},{"issue":"2","key":"10.1016\/j.yrtph.2016.07.013_bib31","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.yrtph.2014.12.008","article-title":"Assessing skin sensitization hazard in mice and men using non-animal test methods","volume":"71","author":"Urbisch","year":"2015","journal-title":"Reg. Toxicol. Pharmacol."},{"issue":"3","key":"10.1016\/j.yrtph.2016.07.013_bib32","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.yrtph.2014.04.018","article-title":"Evaluating the performance of integrated approaches for hazard identification of skin sensitizing chemicals","volume":"69","author":"van der Veen","year":"2014","journal-title":"Reg. Toxicol. Pharmacol."}],"container-title":["Regulatory Toxicology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0273230016301969?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0273230016301969?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T12:28:43Z","timestamp":1759494523000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0273230016301969"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10]]},"references-count":32,"alternative-id":["S0273230016301969"],"URL":"https:\/\/doi.org\/10.1016\/j.yrtph.2016.07.013","relation":{},"ISSN":["0273-2300"],"issn-type":[{"value":"0273-2300","type":"print"}],"subject":[],"published":{"date-parts":[[2016,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Chemical applicability domain of the Local Lymph Node Assay (LLNA) for skin sensitisation potency. Part 2. The biological variability of the murine Local Lymph Node Assay (LLNA) for skin sensitisation","name":"articletitle","label":"Article Title"},{"value":"Regulatory Toxicology and Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.yrtph.2016.07.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}