{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T14:11:03Z","timestamp":1789999863360,"version":"4.0.1"},"reference-count":62,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T00:00:00Z","timestamp":1696896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Regulatory Toxicology and Pharmacology"],"published-print":{"date-parts":[[2023,12]]},"DOI":"10.1016\/j.yrtph.2023.105501","type":"journal-article","created":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T13:18:03Z","timestamp":1696943883000},"page":"105501","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["An international cross-laboratory survey on fish vitellogenin analysis: Methodological challenges and opportunities for best practice"],"prefix":"10.1016","volume":"145","author":[{"given":"Natalie","family":"Burden","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca J.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Breanne","family":"Holmes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace H.","family":"Panter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward R.","family":"Salinas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Sewell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lennart","family":"Weltje","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James R.","family":"Wheeler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Wolf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Lagadic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.yrtph.2023.105501_bib1","doi-asserted-by":"crossref","DOI":"10.1002\/etc.3475","article-title":"Kinetic determination of vitellogenin induction in the epidermis of cyprinid and perciform fishes: evaluation of sensitive enzyme-linked immunosorbent assays","author":"Allner","year":"2016","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.yrtph.2023.105501_bib2","article-title":"Guidance for the identification of endocrine disruptors in the context of Regulations (EU) No 528\/2012 and (EC) No 1107\/2009","author":"Andersson","year":"2018","journal-title":"EFSA J."},{"key":"10.1016\/j.yrtph.2023.105501_bib3","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1002\/etc.2708","article-title":"A novel framework for interpretation of data from the fish short-term reproduction assay (FSTRA) for the detection of endocrine-disrupting chemicals","volume":"33","author":"Ankley","year":"2014","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.yrtph.2023.105501_bib4","article-title":"Limit of blank, limit of detection and limit of quantitation","author":"Armbruster","year":"2008","journal-title":"Clin. Biochem. Rev. PMID"},{"key":"10.1016\/j.yrtph.2023.105501_bib5","doi-asserted-by":"crossref","DOI":"10.5402\/2012\/942804","article-title":"Affinity and matrix effects in measuring fish plasma vitellogenin using immunosorbent assays: considerations for aquatic toxicologists","author":"Bartell","year":"2012","journal-title":"ISRN Toxicol"},{"key":"10.1016\/j.yrtph.2023.105501_bib6","series-title":"Triadimenol \u2013 Fish Sexual Development Test (FSDT) with Fathead Minnow","author":"Bayer","year":"2010"},{"key":"10.1016\/j.yrtph.2023.105501_bib7","volume":"vol. 57377","author":"Bayer","year":"2007"},{"key":"10.1016\/j.yrtph.2023.105501_bib8","doi-asserted-by":"crossref","DOI":"10.1002\/etc.479","article-title":"Estrogenic activity of tropical fish food can alter baseline vitellogenin concentrations in male fathead minnow (Pimephales promelas)","author":"Beresford","year":"2011","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.yrtph.2023.105501_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.yrtph.2023.105501_bib10","doi-asserted-by":"crossref","DOI":"10.1002\/etc.5620210823","article-title":"Development and validation of an enzyme-linked immunosorbent assay to measure vitellogenin in the zebrafish (Danio rerio)","author":"Brion","year":"2002","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.yrtph.2023.105501_bib11","doi-asserted-by":"crossref","DOI":"10.1897\/05-368R.1","article-title":"Plasma sampling and freezing procedures influence vitellogenin measurements by enzyme-linked immunoassay in the fathead minnow (Pimephales promelas)","author":"Brodeur","year":"2006","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.yrtph.2023.105501_bib12","doi-asserted-by":"crossref","DOI":"10.1007\/s10646-019-02128-9","article-title":"Historical control data for the interpretation of ecotoxicity data: are we missing a trick?","author":"Brooks","year":"2019","journal-title":"Ecotoxicology"},{"key":"10.1016\/j.yrtph.2023.105501_bib13","unstructured":"Brown, R., Panter, G., Burden, N., Salinas, E., Weltje, L., Wheeler, J., Wolf, Y., Lagadic, L., n.d. Are Changes in Vitellogenin Concentrations in Fish Reliable Indicators of Chemical-Induced Endocrine Activity? Submitted.."},{"key":"10.1016\/j.yrtph.2023.105501_bib14","doi-asserted-by":"crossref","DOI":"10.1080\/24750263.2019.1631398","article-title":"Vitellogenin gene family in vertebrates: evolution and functions","author":"Carducci","year":"2019","journal-title":"Eur. Zool. J."},{"key":"10.1016\/j.yrtph.2023.105501_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquatox.2011.01.013","article-title":"Effects of the UV-filter 2-ethyl-hexyl-4-trimethoxycinnamate (EHMC) on expression of genes involved in hormonal pathways in fathead minnows (Pimephales promelas) and link to vitellogenin induction and histology","author":"Christen","year":"2011","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.yrtph.2023.105501_bib16","doi-asserted-by":"crossref","DOI":"10.1002\/bdrb.21081","article-title":"Challenges and approaches to conducting and interpreting the amphibian metamorphosis assay and the fish short-term reproduction assay","author":"Coady","year":"2014","journal-title":"Birth Defects Res. Part B Dev. Reproductive Toxicol."},{"key":"10.1016\/j.yrtph.2023.105501_bib17","doi-asserted-by":"crossref","DOI":"10.1021\/acs.estlett.8b00486","article-title":"Unexpected observations: exposure to aromatase inhibitor prochloraz did not alter the vitellogenin content of zebrafish ova but did inhibit the growth of larval offspring","author":"Dang","year":"2018","journal-title":"Environ. Sci. Technol. Lett."},{"key":"10.1016\/j.yrtph.2023.105501_bib18","series-title":"Regulation (EU) No 528\/2012 Concerning the Making Available on the Market and Use of Biocidal Products: Evaluation of Active Substances Assessment Report \u2013 Imiprothrin Product-type 18 (Insecticides, Acaricides and Products to Control Other Arthropods)","year":"2018"},{"key":"10.1016\/j.yrtph.2023.105501_bib19","first-page":"1","article-title":"Commission Regulation (EU) No 283\/2013 of 1 March 2013 setting out the data requirements for active substances, in accordance with Regulation (EC) No 1107\/2009 of the European Parliament and of the Council concerning the placing of plant protection produc","volume":"93","year":"2013","journal-title":"Off. J. Eur. Union"},{"key":"10.1016\/j.yrtph.2023.105501_bib20","volume":"vol. 396","year":"2006"},{"key":"10.1016\/j.yrtph.2023.105501_bib21","series-title":"Recent Updates in Molecular Endocrinology and Reproductive Physiology of Fish","article-title":"Vitellogenesis and their endocrine control in fishes","author":"Gupta","year":"2021"},{"key":"10.1016\/j.yrtph.2023.105501_bib22","doi-asserted-by":"crossref","DOI":"10.1002\/etc.646","article-title":"Effects of \u03b2-endosulfan on the growth and reproduction of zebrafish (Danio rerio)","author":"Han","year":"2011","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.yrtph.2023.105501_bib23","doi-asserted-by":"crossref","DOI":"10.1007\/s12562-015-0957-5","article-title":"Vitellogenesis and choriogenesis in fishes","author":"Hara","year":"2016","journal-title":"Fish. Sci."},{"key":"10.1016\/j.yrtph.2023.105501_bib24","doi-asserted-by":"crossref","DOI":"10.1021\/ac0302859","article-title":"Precision, limit of detection and range of quantitation in competitive ELISA","author":"Hayashi","year":"2004","journal-title":"Anal. Chem."},{"key":"10.1016\/j.yrtph.2023.105501_bib25","article-title":"Mass spectrometry based detection of common vitellogenin peptides across fish species for assessing exposure to estrogenic compounds in aquatic environments","author":"He","year":"2019","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.yrtph.2023.105501_bib26","doi-asserted-by":"crossref","DOI":"10.2166\/wst.2000.0546","article-title":"Vitellogenin as a biomarker for environmental estrogens","author":"Jones","year":"2000","journal-title":"Water Sci. Technol."},{"key":"10.1016\/j.yrtph.2023.105501_bib27","doi-asserted-by":"crossref","DOI":"10.1897\/03-166","article-title":"Surveys of plasma vitellogenin and intersex in male flounder (Platichthys flesus) as measures of endocrine disruption by estrogenic contamination in United Kingdom estuaries: temporal trends, 1996 to 2001","author":"Kirby","year":"2004","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.yrtph.2023.105501_bib28","article-title":"Influence of storage method and duration on plasma vitellogenin concentrations for fathead minnows","author":"Krzykwa","year":"2022","journal-title":"SETAC North Am. 43rd Annu. Meet."},{"key":"10.1016\/j.yrtph.2023.105501_bib29","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1002\/ieam.4156","article-title":"Recommendations for reducing the use of fish and Amphibians in endocrine\u2010disruption testing of biocides and plant protection products in Europe","volume":"15","author":"Lagadic","year":"2019","journal-title":"Integrated Environ. Assess. Manag."},{"key":"10.1016\/j.yrtph.2023.105501_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquatox.2013.03.013","article-title":"Effects of TDCPP or TPP on gene transcriptions and hormones of HPG axis, and their consequences on reproduction in adult zebrafish (Danio rerio)","author":"Liu","year":"2013","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.yrtph.2023.105501_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.yrtph.2018.09.002","article-title":"Distinguishing between endocrine disruption and non-specific effects on endocrine systems","author":"Marty","year":"2018","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"10.1016\/j.yrtph.2023.105501_bib32","doi-asserted-by":"crossref","DOI":"10.1002\/etc.3017","article-title":"Short-term fish reproduction assays with methyl tertiary butyl ether with zebrafish and fathead minnow: implications for evaluation of potential for endocrine activity","author":"Mihaich","year":"2015","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.yrtph.2023.105501_bib33","series-title":"Environmental Toxicology and Chemistry","article-title":"Long-term exposure to environmentally relevant doses of methyl-tert-butyl ether causes significant reproductive dysfunction in the zebrafish (Danio rerio)","author":"Moreels","year":"2006"},{"key":"10.1016\/j.yrtph.2023.105501_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/S1532-0456(02)00273-9","article-title":"Comparison of ELISAs for detecting vitellogenin in the fathead minnow (Pimephales promelas)","author":"Mylchreest","year":"2003","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"10.1016\/j.yrtph.2023.105501_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquatox.2014.01.009","article-title":"Developmental exposure of zebrafish (Danio rerio) to bisphenol-S impairs subsequent reproduction potential and hormonal balance in adults","author":"Naderi","year":"2014","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.yrtph.2023.105501_bib36","series-title":"Revised Guidance Document 150 on Standardised Test Guidelines for Evaluating Chemicals for Endocrine Disruption, OECD Series on Testing and Assessment","year":"2018"},{"key":"10.1016\/j.yrtph.2023.105501_bib37","series-title":"Test No. 240: Medaka Extended One Generation Reproduction Test (MEOGRT), OECD Guidelines for the Testing of Chemicals, Section 2","year":"2015"},{"key":"10.1016\/j.yrtph.2023.105501_bib38","series-title":"Test No. 241 the Larval Amphibian Growth and Development Assay (LAGDA), OECD Guidelines for the Testing of Chemicals Section 2","year":"2015"},{"key":"10.1016\/j.yrtph.2023.105501_bib39","series-title":"Test No 234: Fish Sexual Development Test, OECD Guideline for the Testing of Chemicals Section 2","year":"2011"},{"key":"10.1016\/j.yrtph.2023.105501_bib40","series-title":"OECD Guidelines for the Testing of Chemicals Section 2","article-title":"Test No. 229: fish short term reproduction assay, test No. 229: fish short term reproduction assay","year":"2009"},{"key":"10.1016\/j.yrtph.2023.105501_bib41","series-title":"OECD Guidelines for the Testing of Chemicals Section 2","article-title":"Test No. 230: 21-Day Fish Assay: a short-term screening for estrogenic and androgenic activity, and aromatase inhibition","year":"2009"},{"key":"10.1016\/j.yrtph.2023.105501_bib42","article-title":"Final report of the validation of the 21-day fish screening assay for the detection of endocrine active substances","volume":"vol. 78","year":"2007"},{"key":"10.1016\/j.yrtph.2023.105501_bib43","series-title":"Report of the Initial Work towards the Validation of the 21-day Fish Screening Assay for the Detection of Endocrine Active Substances - Phase 1A","first-page":"60","year":"2006"},{"key":"10.1016\/j.yrtph.2023.105501_bib44","series-title":"Report of the Validation of the 21-day Fish Screening Assay for the Detection of Endocrine Active Substances - Phase 1B","first-page":"61","year":"2006"},{"key":"10.1016\/j.yrtph.2023.105501_bib45","doi-asserted-by":"crossref","DOI":"10.1016\/S1081-1206(10)60595-9","article-title":"The logarithmic transformation and the geometric mean in reporting experimental IgE results: what are they and when and why to use them?","author":"Olivier","year":"2008","journal-title":"Ann. Allergy Asthma Immunol."},{"key":"10.1016\/j.yrtph.2023.105501_bib46","doi-asserted-by":"crossref","DOI":"10.1002\/jat.4104","article-title":"Summary of reference chemicals evaluated by the fish short-term reproduction assay, OECD TG229, using Japanese Medaka, Oryzias latipes","author":"Onishi","year":"2021","journal-title":"J. Appl. Toxicol."},{"key":"10.1016\/j.yrtph.2023.105501_bib47","doi-asserted-by":"crossref","DOI":"10.1016\/0003-2697(77)90723-0","article-title":"An evaluation of the Coomassie brilliant blue G-250 dye-binding method for quantitative protein determination","author":"Pierce","year":"1977","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.yrtph.2023.105501_bib48","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2018.09.120","article-title":"Two-generational reproductive toxicity assessment of 6:2 chlorinated polyfluorinated ether sulfonate (F-53B, a novel alternative to perfluorooctane sulfonate) in zebrafish","author":"Shi","year":"2018","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.yrtph.2023.105501_bib49","series-title":"Report: Advice on Fish and Amphibian Vitellogenin Assays Used for the Regulation of Endocrine Disrupting Substances","year":"2020"},{"key":"10.1016\/j.yrtph.2023.105501_bib50","series-title":"Endocrine Disruptor Screening Program Test Guidelines - 890.2200: Medaka Extended One Generation Reproduction Test (MEOGRT)","year":"2014"},{"key":"10.1016\/j.yrtph.2023.105501_bib51","series-title":"Endocrine Disruptor Screening Program Test Guidelines - 890.1200: Aromatase (Human Recombinant)","year":"2009"},{"key":"10.1016\/j.yrtph.2023.105501_bib52","series-title":"A Short-Term Test Method for Assessing the Reproductive Toxicity of Endocrine-Disrupting Chemicals Using the Fathead Minnow (Pimephales promelas)","year":"2002"},{"key":"10.1016\/j.yrtph.2023.105501_bib53","series-title":"Ecological Effects Test Guidelines - 850.1500 Fish Life Cycle Toxicity. [EPA712\u2013C\u201396\u2013122] USEPA","year":"1996"},{"key":"10.1016\/j.yrtph.2023.105501_bib54","doi-asserted-by":"crossref","DOI":"10.1002\/ieam.1747","article-title":"Are all chemicals endocrine disruptors?","author":"Wheeler","year":"2016","journal-title":"Integrated Environ. Assess. Manag."},{"key":"10.1016\/j.yrtph.2023.105501_bib55","doi-asserted-by":"crossref","DOI":"10.1016\/j.envint.2020.106287","article-title":"Hormone data collection in support of endocrine disruption (ED) assessment for aquatic vertebrates: pragmatic and animal welfare considerations","author":"Wheeler","year":"2021","journal-title":"Environ. Int."},{"key":"10.1016\/j.yrtph.2023.105501_bib56","doi-asserted-by":"crossref","DOI":"10.1080\/15376520590968789","article-title":"Vitellogenin: a review of analytical methods to detect (anti) estrogenic activity in fish","author":"Wheeler","year":"2005","journal-title":"Toxicol. Mech. Methods"},{"key":"10.1016\/j.yrtph.2023.105501_bib57","doi-asserted-by":"crossref","DOI":"10.1016\/j.yrtph.2019.104424","article-title":"Control performance of fish short term reproduction assays with fathead minnow (Pimephales promelas)","author":"Wheeler","year":"2019","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"10.1016\/j.yrtph.2023.105501_bib58","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquatox.2005.03.015","article-title":"Method for detection and quantitation of fathead minnow vitellogenin (Vtg) by liquid chromatography and matrix-assisted laser desorption\/ionization mass spectrometry","author":"Wunschel","year":"2005","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.yrtph.2023.105501_bib59","doi-asserted-by":"crossref","DOI":"10.1897\/03-425","article-title":"Quantitative measurement of fathead minnow vitellogenin by liquid chromatography combined with tandem mass spectrometry using a signature peptide of vitellogenin","author":"Zhang","year":"2004","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.yrtph.2023.105501_bib60","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2015.08.011","article-title":"Vitellogenin is an immunocompetent molecule for mother and offspring in fish","author":"Zhang","year":"2015","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.yrtph.2023.105501_bib61","doi-asserted-by":"crossref","DOI":"10.1016\/j.ygcen.2017.07.032","article-title":"Estrogenic effects associated with bisphenol a exposure in male zebrafish (Danio rerio) is associated with changes of endogenous 17\u03b2-estradiol and gene specific DNA methylation levels","author":"Zhao","year":"2017","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.yrtph.2023.105501_bib62","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquatox.2016.12.011","article-title":"Quizalofop-P-ethyl exposure increases estrogen axis activity in male and slightly decreases estrogen axis activity in female zebrafish (Danio rerio)","author":"Zhu","year":"2017","journal-title":"Aquat. Toxicol."}],"container-title":["Regulatory Toxicology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0273230023001691?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0273230023001691?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T12:36:08Z","timestamp":1759494968000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0273230023001691"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12]]},"references-count":62,"alternative-id":["S0273230023001691"],"URL":"https:\/\/doi.org\/10.1016\/j.yrtph.2023.105501","relation":{},"ISSN":["0273-2300"],"issn-type":[{"value":"0273-2300","type":"print"}],"subject":[],"published":{"date-parts":[[2023,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An international cross-laboratory survey on fish vitellogenin analysis: Methodological challenges and opportunities for best practice","name":"articletitle","label":"Article Title"},{"value":"Regulatory Toxicology and Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.yrtph.2023.105501","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"105501"}}