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At 20 min postinjection 3 endo\u2010\u03b2\u2010<jats:italic>N<\/jats:italic>\u2010acetylglucosaminidase H\u2010sensitive bands were observed representing the propeptide (<jats:italic>M<\/jats:italic>\n<jats:sub>r<\/jats:sub> 75 000) large subunit (<jats:italic>M<\/jats:italic>\n<jats:sub>r<\/jats:sub> 49 500) and small subunit (<jats:italic>M<\/jats:italic>\n<jats:sub>r<\/jats:sub> 29 000) of transpeptidase. The alterations in <jats:italic>M<\/jats:italic>\n<jats:sub>r<\/jats:sub> are consistent with removal of 6 N\u2010linked coreoligosaccharides from the propeptide; 4 from the large subunit and 2 from the small subunit. All 3 bands became more diffuse and less endoglycosidase H\u2010sensitive by 40 min and completely resistant by 60 min postinjection. At 20 h postinjection no propeptide remained. Thus, the primary propeptide cleavage reaction occurs prior to the loss of endoglycosidase H sensitivity while about 30% of the propeptide is processed along with the heterodimer and cleaved at a later time.<\/jats:p>","DOI":"10.1016\/0014-5793(83)81132-6","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:59:44Z","timestamp":1027583984000},"page":"139-143","source":"Crossref","is-referenced-by-count":30,"title":["Processing of the propeptide form of rat renal \u03b3\u2010glutamyltranspeptidase"],"prefix":"10.1002","volume":"157","author":[{"given":"Michael A.","family":"Capraro","sequence":"first","affiliation":[]},{"given":"Rebecca P.","family":"Hughey","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,10,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)43887-8"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-711X(80)90005-1"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)57014-4"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)33164-8"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.3.931"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1977.tb11323.x"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(82)90323-6"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)33380-5"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)68229-3"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1975.tb02238.x"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)86009-X"},{"key":"e_1_2_1_14_1","first-page":"12","volume-title":"The Biochemistry of Glycoproteins and Proteoglycans","author":"Kornfeld R.","year":"1980"},{"key":"e_1_2_1_15_1","first-page":"221","volume-title":"The Biochemistry of Glycoproteins and Proteoglycans","author":"Atkinson P.H.","year":"1980"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)34177-7"},{"key":"e_1_2_1_17_1","first-page":"235","volume-title":"Multifunctional Proteins","author":"Hughey R.P.","year":"1980"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)34819-1"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.1.134"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)43217-6"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)86501-8"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)68581-9"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)32879-5"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793%2883%2981132-6","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793(83)81132-6","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/0014-5793%2883%2981132-6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T11:20:55Z","timestamp":1694863255000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/0014-5793%2883%2981132-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,6,27]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1983,6,27]]}},"alternative-id":["10.1016\/0014-5793(83)81132-6"],"URL":"https:\/\/doi.org\/10.1016\/0014-5793(83)81132-6","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,6,27]]}}}