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The relationship between the two mRNAs of different size and the active enzyme is not clear, nor is the cellular localization of the latter known. We have cloned a cDNA corresponding to the 1.6 kb RNA. This cDNA was sequenced and its complete coding region was subcloned into pSP64. The resulting plasmid was used to direct the synthesis of micrograms of capped RNA transcript after linearization in the 3'\u2010non\u2010coding region. A 39 kDa protein was synthesized when this RNA was translated in rabbit reticulocyte lysate. When this capped RNA was introduced by microinjection into <jats:italic>Xenopus<\/jats:italic> oocytes, production of 2\u20135A synthetase was clearly observed in the cytoplasm and 10\u201330% of the enzyme accumulated with time in the nucleoplasm. Analysis of cytoplasmic homogenates of these oocytes on a glycerol gradient revealed that the enzyme is fully active in the monomeric form.<\/jats:p>","DOI":"10.1016\/0014-5793(86)80224-1","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:57:58Z","timestamp":1027583878000},"page":"113-120","source":"Crossref","is-referenced-by-count":35,"title":["Full\u2010length sequence and expression of the 42 kDa 2\u20135A synthetase induced by human interferon"],"prefix":"10.1002","volume":"196","author":[{"given":"M.","family":"Wathelet","sequence":"first","affiliation":[]},{"given":"S.","family":"Moutschen","sequence":"additional","affiliation":[]},{"given":"A.","family":"Cravador","sequence":"additional","affiliation":[]},{"given":"L.","family":"DeWit","sequence":"additional","affiliation":[]},{"given":"P.","family":"Defilippi","sequence":"additional","affiliation":[]},{"given":"G.","family":"Huez","sequence":"additional","affiliation":[]},{"given":"J.","family":"Content","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,10,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(84)90036-6"},{"key":"e_1_2_1_3_1","first-page":"283","volume":"82","author":"Galabru J.","year":"1984","journal-title":"Bull. 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