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Cells were labelled with <jats:sup>32<\/jats:sup>PO<jats:sub>4<\/jats:sub>\n<jats:sup>3\u2212<\/jats:sup>, lysed, the receptor immunoprecipitated with specific anti\u2010peptide antibodies and the immunoprecipitates analysed by SDS\u2010PAGE followed by autoradiography. A significant basal level of receptor phosphorylation was observed which was rapidly and transiently increased in response to agonist activation of the receptor. This agonist effect was found to be dose dependent with a rapid time course and could be abolished by the specific PKC inhibitor Ro318220, suggesting that PKC was responsible for the agonist mediated phosphorylation of the receptor.<\/jats:p>","DOI":"10.1016\/0014-5793(95)00575-t","type":"journal-article","created":{"date-parts":[[2003,5,13]],"date-time":"2003-05-13T01:07:27Z","timestamp":1052788047000},"page":"301-305","source":"Crossref","is-referenced-by-count":48,"title":["Rapid agonist mediated phosphorylation of the metabotropic glutamate receptor 1\u03b1 by protein kinase C in permanently transfected BHK cells"],"prefix":"10.1002","volume":"367","author":[{"given":"Simon","family":"Alaluf","sequence":"first","affiliation":[]},{"given":"Eileen R.","family":"Mulvihill","sequence":"additional","affiliation":[]},{"given":"Robert A.J.","family":"McIlhinney","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2000,3,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90118-W"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/349760a0"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1656524"},{"key":"e_1_2_1_5_1","first-page":"10331","volume-title":"Proc. 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