{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T12:59:00Z","timestamp":1771678740737,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[1999,11,30]],"date-time":"1999-11-30T00:00:00Z","timestamp":943920000000},"content-version":"vor","delay-in-days":1373,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1996,2,26]]},"abstract":"<jats:p>Airway epithelial cells bearing mutations of the cystic fibrosis (CF) transmembrane conductance regulator (CFTR) possess an increased Na<jats:sup>+<\/jats:sup> conductance along with their well described defect of cAMP dependent Cl<jats:sup>\u2212<\/jats:sup> conductance. Currently it is not clear, how this occurs, and whether it is due to a CFTR control of epithelial Na<jats:sup>+<\/jats:sup> conductances which might be defective in CF patients. In the present study, we have tried to identify possible interactions between both CFTR and the epithelial Na<jats:sup>+<\/jats:sup> conductance by overexpressing respective cRNAs in <jats:italic>Xenopus<\/jats:italic> oocytes. The expression of all three (\u03b1, \u03b2, \u03b3) subunits of the rat epithelial Na<jats:sup>+<\/jats:sup> channel (rENaC) and wild type (wt) CFTR resulted in the expected amiloride sensitive Na<jats:sup>+<\/jats:sup> and IBMX (1 mmol\/l) activated Cl<jats:sup>\u2212<\/jats:sup> currents, respectively. The amiloride sensitive Na<jats:sup>+<\/jats:sup> conductance was, however, inhibited when the wt\u2010CFTR Cl<jats:sup>\u2212<\/jats:sup> conductance was activated by phosphodiesterase inhibition (IBMX). In contrast, IBMX had no such effect in \u0394F508 and Na<jats:sup>+<\/jats:sup> channels coexpressing oocytes. These results suggest that wt\u2010CFTR, but not \u0394F508\u2010CFTR, is a cAMP dependent downregulator of epithelial Na<jats:sup>+<\/jats:sup> channels. This may explain the higher Na<jats:sup>+<\/jats:sup> conductance observed in airway epithelial cells of CF patients.<\/jats:p>","DOI":"10.1016\/0014-5793(96)00079-8","type":"journal-article","created":{"date-parts":[[2003,4,5]],"date-time":"2003-04-05T00:21:01Z","timestamp":1049502061000},"page":"47-52","source":"Crossref","is-referenced-by-count":118,"title":["Wild type but not \u0394F508 CFTR inhibits Na<sup>+<\/sup> conductance when coexpressed in <i>Xenopus<\/i> oocytes"],"prefix":"10.1002","volume":"381","author":[{"given":"M.","family":"Mall","sequence":"first","affiliation":[]},{"given":"A.","family":"Hipper","sequence":"additional","affiliation":[]},{"given":"R.","family":"Greger","sequence":"additional","affiliation":[]},{"given":"K.","family":"Kunzelmann","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1999,11,30]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00374233"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1712984"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112708"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1373908"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/367463a0"},{"key":"e_1_2_1_7_1","author":"Ecke D.","year":"1996","journal-title":"Pfluegers Arch. 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