{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T05:25:28Z","timestamp":1787117128958,"version":"build-2736575974"},"reference-count":35,"publisher":"Elsevier BV","issue":"3-4","license":[{"start":{"date-parts":[[1981,12,1]],"date-time":"1981-12-01T00:00:00Z","timestamp":376012800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular and Biochemical Parasitology"],"published-print":{"date-parts":[[1981,12]]},"DOI":"10.1016\/0166-6851(81)90021-9","type":"journal-article","created":{"date-parts":[[2002,12,28]],"date-time":"2002-12-28T08:15:02Z","timestamp":1041063302000},"page":"225-242","source":"Crossref","is-referenced-by-count":22,"title":["Biosynthesis of Trypanosoma brucei variant surface glycoprotein. I. Synthesis, size, and processing of an N-terminal signal peptide"],"prefix":"10.1016","volume":"4","author":[{"given":"Josie","family":"McConnell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne M.","family":"Gurnett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John S.","family":"Cordingley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John E.","family":"Walker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mervyn J.","family":"Turner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/0166-6851(81)90021-9_BIB1","series-title":"The Molecular Basis of the Infective Process","first-page":"133","article-title":"Antigenic variation","author":"Turner","year":"1979"},{"key":"10.1016\/0166-6851(81)90021-9_BIB2","first-page":"133","article-title":"Identification, purification and preparation of clone-specific glycoprotein antigens constituting the surface coat of T. brucei","volume":"75","author":"Cross","year":"1975","journal-title":"Parasitology"},{"key":"10.1016\/0166-6851(81)90021-9_BIB3","series-title":"Biochemistry of Parasites and Host\u2014Parasite Relationships","first-page":"413","article-title":"Structure and organisation of the variant-specific surface antigens of Trypanosoma brucei","author":"Cross","year":"1976"},{"key":"10.1016\/0166-6851(81)90021-9_BIB4","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/0020-7519(73)90095-7","article-title":"Changes in oxidative metabolism and ultrstructure accompanying differentiation of the mitochondrion in Trypanosoma brucei","volume":"3","author":"Brown","year":"1973","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/0166-6851(81)90021-9_BIB5","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1017\/S0031182000082639","article-title":"Antigenic similarity among culture forms of the brucei group of trypanosomes","volume":"54","author":"Seed","year":"1964","journal-title":"Parasitology"},{"key":"10.1016\/0166-6851(81)90021-9_BIB6","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/0035-9203(81)90106-1","article-title":"Absence of a surface coat from metacyclic Trypanosoma vivax: possible implications for vaccinations against vivax trypanosomiasis","volume":"75","author":"Tetley","year":"1981","journal-title":"Trans. Roy. Soc. Trop. Med. Hyg."},{"key":"10.1016\/0166-6851(81)90021-9_BIB7","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0166-6851(80)90011-0","article-title":"Isolation of Trypanosoma brucei variant specific antigen mRNA by immunoprecipitation of polysomes","volume":"1","author":"Cordingley","year":"1980","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/0166-6851(81)90021-9_BIB8","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/0378-1119(80)90043-8","article-title":"The isolation of plasmids containing DNA complementary to mRNA for VSGs of T. brucei","volume":"8","author":"Hoeijmakers","year":"1980","journal-title":"Gene"},{"key":"10.1016\/0166-6851(81)90021-9_BIB9","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1111\/j.1550-7408.1977.tb01020.x","article-title":"Carbohydrate composition of variant-specific surface antigen glycoproteins from Trypanosoma brucei","volume":"24","author":"Johnson","year":"1977","journal-title":"J. Protozool."},{"key":"10.1016\/0166-6851(81)90021-9_BIB10","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1038\/288624a0","article-title":"A variant surface glycoprotein of Trypanosoma brucei synthesised with a C-terminal hydrophobic \u2018tail\u2019 absent from purified glycoprotein","volume":"288","author":"Boothroyd","year":"1980","journal-title":"Nature (London)"},{"key":"10.1016\/0166-6851(81)90021-9_BIB11","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1017\/S0031182000041871","article-title":"Analysis of antigenic types appearing in first relapse populations of clones of Trypanosoma brucei","volume":"82","author":"Miller","year":"1981","journal-title":"Parasitology"},{"key":"10.1016\/0166-6851(81)90021-9_BIB12","first-page":"467","article-title":"Proposals for the nomenclature of salivarian trypanosomes and for the maintenance of reference collections","volume":"56","author":"Lumsden","year":"1978","journal-title":"Bull. W.H.O."},{"key":"10.1016\/0166-6851(81)90021-9_BIB13","first-page":"293","article-title":"Introduction to radioimmunoassay and related techniques","volume":"Vol. 6","author":"Chard","year":"1978"},{"key":"10.1016\/0166-6851(81)90021-9_BIB14","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1017\/S0031182000061928","article-title":"Isolation and characterisation of polysomes from Trypanosoma brucei","volume":"81","author":"Cordingley","year":"1980","journal-title":"Parasitology"},{"key":"10.1016\/0166-6851(81)90021-9_BIB15","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/0014-4894(70)90120-7","article-title":"Isolation of salivarian trypanosomes from man and other mammals using DEA cellulose","volume":"28","author":"Lanham","year":"1970","journal-title":"Exp. Parasitol."},{"key":"10.1016\/0166-6851(81)90021-9_BIB16","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1111\/j.1432-1033.1976.tb10656.x","article-title":"An efficient mRNA dependent translation system from reticulocyte lysates","volume":"67","author":"Pelham","year":"1976","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/0166-6851(81)90021-9_BIB17","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature (London)"},{"key":"10.1016\/0166-6851(81)90021-9_BIB18","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.4049\/jimmunol.115.6.1617","article-title":"Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent","volume":"115","author":"Kessler","year":"1975","journal-title":"J. Immunol."},{"key":"10.1016\/0166-6851(81)90021-9_BIB19","first-page":"238","article-title":"The cyanogen bromide reaction","volume":"Vol. 11","author":"Cross","year":"1967"},{"key":"10.1016\/0166-6851(81)90021-9_BIB20","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1111\/j.1432-1033.1974.tb03599.x","article-title":"A film detection method for tritium labelled protein and nucleic acids in polyacrylamide gels","volume":"46","author":"Bonner","year":"1974","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/0166-6851(81)90021-9_BIB21","series-title":"An approach to the rapid determination of an amino acid sequence with small amounts of material","first-page":"185","article-title":"Triose phosphate isomerase from the Coelacanth","volume":"137","author":"Kolb","year":"1974"},{"key":"10.1016\/0166-6851(81)90021-9_BIB22","doi-asserted-by":"crossref","first-page":"5547","DOI":"10.1016\/S0021-9258(19)43638-7","article-title":"A \u03b2-mannosidic linkage in the Unit A oligosaccharide of bovine thyroglobulin","volume":"248","author":"Tarentuie","year":"1973","journal-title":"J. Biol. Chem."},{"key":"10.1016\/0166-6851(81)90021-9_BIB23","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/194495a0","article-title":"Preparation of iodine-131 labelled human growth hormone of high specific activity","volume":"194","author":"Hunter","year":"1962","journal-title":"Nature (London)"},{"key":"10.1016\/0166-6851(81)90021-9_BIB24","first-page":"99","article-title":"Mechanisms for the intracellular compartmentation of newly synthesised proteins","volume":"Vol. 43","author":"Blobel","year":"1978"},{"key":"10.1016\/0166-6851(81)90021-9_BIB25","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1042\/bj1780689","article-title":"Selective cleavage of variant surface glycoproteins from Trypanosoma brucei","volume":"178","author":"Johnson","year":"1979","journal-title":"Biochem. J."},{"key":"10.1016\/0166-6851(81)90021-9_BIB26","series-title":"C-terminal location of antigenically cross-reacting carbohydrate moieties","first-page":"135","article-title":"Glycopeptides from variant surface glycoproteins of Trypanosoma brucei","volume":"2","author":"Holder","year":"1981"},{"key":"10.1016\/0166-6851(81)90021-9_BIB27","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1038\/263613a0","article-title":"N-terminal amino acid sequences of variant-specific surface antigens from Trypanosoma brucei","volume":"263","author":"Bridgen","year":"1976","journal-title":"Nature (London)"},{"key":"10.1016\/0166-6851(81)90021-9_BIB28","doi-asserted-by":"crossref","first-page":"6568","DOI":"10.1016\/S0021-9258(19)46969-X","article-title":"Migration of 40S ribosomal subunits on messenger RNA in the presence of edeine","volume":"253","author":"Kozak","year":"1978","journal-title":"J. Biol. Chem."},{"key":"10.1016\/0166-6851(81)90021-9_BIB29","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1093\/nar\/7.3.595","article-title":"Immunological purification and partial characterisation of VSSA mRNA of T. brucei brucei","volume":"7","author":"Lheureux","year":"1979","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/0166-6851(81)90021-9_BIB30","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1083\/jcb.79.2.567","article-title":"Nascent chicken ovalbumin contains the functional equivalent of a signal sequence","volume":"79","author":"Lingappa","year":"1978","journal-title":"J. Cell Biol."},{"key":"10.1016\/0166-6851(81)90021-9_BIB31","series-title":"Non-destructive disassembly of rat liver microsomes into ribosomal and membranous components","first-page":"206","article-title":"Ribosome\u2014membrane interactions","volume":"56","author":"Adelman","year":"1973"},{"key":"10.1016\/0166-6851(81)90021-9_BIB32","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/0968-0004(79)90081-1","article-title":"Protein biosynthesis on membrane-bound ribosomes","volume":"4","author":"Leader","year":"1979","journal-title":"Trends Biol. Sci."},{"key":"10.1016\/0166-6851(81)90021-9_BIB33","doi-asserted-by":"crossref","first-page":"5215","DOI":"10.1021\/bi00617a022","article-title":"Complete amino acid sequence of the precursor region of rat prolactin","volume":"17","author":"McKean","year":"1978","journal-title":"Biochemistry"},{"key":"10.1016\/0166-6851(81)90021-9_BIB34","doi-asserted-by":"crossref","unstructured":"Boothroyd, J.C., Paynter, C.A., Cross, G.A.M., Bernards, A. and Borst, P. Variant surface glycoproteins of Trypanosoma brucei are synthesised with cleavable hydrophobic sequences at the carboxy termini. Submitted to Nucleic Acids Res.","DOI":"10.1093\/nar\/9.18.4735"},{"key":"10.1016\/0166-6851(81)90021-9_BIB35","series-title":"II. Reconstitution of functional rough microsomes from heterologous components","first-page":"852","article-title":"Transfer of proteins across membranes","volume":"67","author":"Blobel","year":"1975"}],"container-title":["Molecular and Biochemical Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:0166685181900219?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:0166685181900219?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T15:49:41Z","timestamp":1704728981000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/0166685181900219"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,12]]},"references-count":35,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[1981,12]]}},"alternative-id":["0166685181900219"],"URL":"https:\/\/doi.org\/10.1016\/0166-6851(81)90021-9","relation":{},"ISSN":["0166-6851"],"issn-type":[{"value":"0166-6851","type":"print"}],"subject":[],"published":{"date-parts":[[1981,12]]}}}