{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T12:59:18Z","timestamp":1761310758732},"reference-count":46,"publisher":"Elsevier","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012]]},"DOI":"10.1016\/b978-0-12-391857-4.00007-0","type":"book-chapter","created":{"date-parts":[[2012,1,18]],"date-time":"2012-01-18T23:03:12Z","timestamp":1326927792000},"page":"147-161","source":"Crossref","is-referenced-by-count":6,"title":["Fluorescent Speckle Microscopy in Cultured Cells"],"prefix":"10.1016","author":[{"given":"Marin","family":"Barisic","sequence":"first","affiliation":[]},{"given":"Ant\u00f3nio J.","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Helder","family":"Maiato","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0005","doi-asserted-by":"crossref","first-page":"2185","DOI":"10.1126\/science.287.5461.2185","article-title":"The genome sequence of Drosophila melanogaster","volume":"287","author":"Adams","year":"2000","journal-title":"Science"},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0010","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1111\/j.0022-2720.2004.01408.x","article-title":"Signal analysis of total internal reflection fluorescent speckle microscopy (TIR-FSM) and wide-field epi-fluorescence FSM of the actin cytoskeleton and focal adhesions in living cells","volume":"216","author":"Adams","year":"2004","journal-title":"J. Microsc."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0015","doi-asserted-by":"crossref","first-page":"3967","DOI":"10.1091\/mbc.02-05-0069","article-title":"Microtubule flux and sliding in mitotic spindles of Drosophila embryos","volume":"13","author":"Brust-Mascher","year":"2002","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0020","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1091\/mbc.E08-10-1033","article-title":"Kinesin-5-dependent poleward flux and spindle length control in Drosophila embryo mitosis","volume":"20","author":"Brust-Mascher","year":"2009","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0025","doi-asserted-by":"crossref","first-page":"3885","DOI":"10.1242\/jcs.114.21.3885","article-title":"Rapid dynamics of the microtubule binding of ensconsin in vivo","volume":"114","author":"Bulinski","year":"2001","journal-title":"J. Cell Sci."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0030","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1083\/jcb.200511112","article-title":"A new method reveals microtubule minus ends throughout the meiotic spindle","volume":"175","author":"Burbank","year":"2006","journal-title":"J. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0035","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1016\/j.cub.2007.07.058","article-title":"Slide-and-cluster models for spindle assembly","volume":"17","author":"Burbank","year":"2007","journal-title":"Curr. Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0040","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1083\/jcb.200601075","article-title":"Kinesin 5-independent poleward flux of kinetochore microtubules in PtK1 cells","volume":"173","author":"Cameron","year":"2006","journal-title":"J. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0045","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1046\/j.1365-2818.2003.01222.x","article-title":"Quantitative fluorescent speckle microscopy: Where it came from and where it is going","volume":"211","author":"Danuser","year":"2003","journal-title":"J. Microsc."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0050","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1146\/annurev.biophys.35.040405.102114","article-title":"Quantitative fluorescent speckle microscopy of cytoskeleton dynamics","volume":"35","author":"Danuser","year":"2006","journal-title":"Annu. Rev. Biophys. Biomol. Struct."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0055","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1747-1028-6-8","article-title":"Anaphase B spindle dynamics in Drosophila S2 cells: Comparison with embryo spindles","volume":"6","author":"de Lartigue","year":"2011","journal-title":"Cell Div."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0060","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1016\/j.cub.2005.08.065","article-title":"Efficient mitosis in human cells lacking poleward microtubule flux","volume":"15","author":"Ganem","year":"2005","journal-title":"Curr. Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0065","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1016\/j.cub.2005.09.054","article-title":"Length control of the metaphase spindle","volume":"15","author":"Goshima","year":"2005","journal-title":"Curr. Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0070","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1083\/jcb.111.3.1039","article-title":"Kinetochores capture astral microtubules during chromosome attachment to the mitotic spindle: Direct visualization in live newt lung cells","volume":"111","author":"Hayden","year":"1990","journal-title":"J. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0075","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1038\/nrm2713","article-title":"Growth, fluctuation and switching at microtubule plus ends","volume":"10","author":"Howard","year":"2009","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0080","doi-asserted-by":"crossref","first-page":"R22","DOI":"10.1016\/S0960-9822(99)00251-1","article-title":"Speckle microscopy: When less is more","volume":"10","author":"Keating","year":"2000","journal-title":"Curr. Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0085","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.molcel.2004.07.012","article-title":"Mitotic spindle assembly and chromosome segregation: Refocusing on microtubule dynamics","volume":"15","author":"Kline-Smith","year":"2004","journal-title":"Mol. Cell"},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0090","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1038\/nmeth.1324","article-title":"Super-resolution video microscopy of live cells by structured illumination","volume":"6","author":"Kner","year":"2009","journal-title":"Nat. Methods"},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0095","doi-asserted-by":"crossref","first-page":"5724","DOI":"10.1091\/mbc.E04-08-0750","article-title":"Direct visualization of microtubule flux during metaphase and anaphase in crane-fly spermatocytes","volume":"15","author":"LaFountain","year":"2004","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0100","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1126\/science.1082520","article-title":"Development and use of fluorescent protein markers in living cells","volume":"300","author":"Lippincott-Schwartz","year":"2003","journal-title":"Science"},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0105","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1083\/jcb.144.5.977","article-title":"Microtubule dynamics from mating through the first zygotic division in the budding yeast Saccharomyces cerevisiae","volume":"144","author":"Maddox","year":"1999","journal-title":"J. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0110","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1038\/71357","article-title":"The polarity and dynamics of microtubule assembly in the budding yeast Saccharomyces cerevisiae","volume":"2","author":"Maddox","year":"2000","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0115","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1083\/jcb.200301088","article-title":"Direct observation of microtubule dynamics at kinetochores in Xenopus extract spindles: Implications for spindle mechanics","volume":"162","author":"Maddox","year":"2003","journal-title":"J. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0120","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1159\/000323803","article-title":"Prevention and correction mechanisms behind anaphase synchrony: Implications for the genesis of aneuploidy","volume":"133","author":"Matos","year":"2011","journal-title":"Cytogenet. Genome Res."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0125","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1083\/jcb.200904153","article-title":"Synchronizing chromosome segregation by flux-dependent force equalization at kinetochores","volume":"186","author":"Matos","year":"2009","journal-title":"J. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0130","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1083\/jcb.109.2.637","article-title":"Polewards microtubule flux in the mitotic spindle: Evidence from photoactivation of fluorescence","volume":"109","author":"Mitchison","year":"1989","journal-title":"J. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0135","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1038\/312237a0","article-title":"Dynamic instability of microtubule growth","volume":"312","author":"Mitchison","year":"1984","journal-title":"Nature"},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0140","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1083\/jcb.200407126","article-title":"The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles","volume":"167","author":"Miyamoto","year":"2004","journal-title":"J. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0145","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/S0091-679X(10)97014-3","article-title":"Drosophila S2 cells as a model system to investigate mitotic spindle dynamics, architecture, and function","volume":"97","author":"Moutinho-Pereira","year":"2010","journal-title":"Methods Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0150","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.ymeth.2010.01.016","article-title":"Improved kymography tools and its applications to mitosis","volume":"51","author":"Pereira","year":"2010","journal-title":"Methods"},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0155","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/978-1-60327-993-2_9","article-title":"Dissecting mitosis with laser microsurgery and RNAi in Drosophila cells","volume":"545","author":"Pereira","year":"2009","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0160","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/S0092-8674(00)80656-X","article-title":"CLIP-170 highlights growing microtubule ends in vivo","volume":"96","author":"Perez","year":"1999","journal-title":"Cell"},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0165","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1083\/jcb.200203022","article-title":"Dual-wavelength fluorescent speckle microscopy reveals coupling of microtubule and actin movements in migrating cells","volume":"158","author":"Salmon","year":"2002","journal-title":"J. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0170","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S0006-3495(95)79937-4","article-title":"Real time imaging of single fluorophores on moving actin with an epifluorescence microscope","volume":"69","author":"Sase","year":"1995","journal-title":"Biophys. J."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0175","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1038\/352126a0","article-title":"Actin microfilament dynamics in locomoting cells","volume":"352","author":"Theriot","year":"1991","journal-title":"Nature"},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0180","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1083\/jcb.102.3.1032","article-title":"Analysis of the treadmilling model during metaphase of mitosis using fluorescence redistribution after photobleaching","volume":"102","author":"Wadsworth","year":"1986","journal-title":"J. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0185","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1083\/jcb.101.2.597","article-title":"Exchange of actin subunits at the leading edge of living fibroblasts: Possible role of treadmilling","volume":"101","author":"Wang","year":"1985","journal-title":"J. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0190","doi-asserted-by":"crossref","first-page":"R633","DOI":"10.1016\/S0960-9822(02)01139-9","article-title":"New directions for fluorescent speckle microscopy","volume":"12","author":"Waterman-Storer","year":"2002","journal-title":"Curr. Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0195","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1083\/jcb.139.2.417","article-title":"Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling","volume":"139","author":"Waterman-Storer","year":"1997","journal-title":"J. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0200","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.1016\/S0006-3495(98)77648-9","article-title":"How microtubules get fluorescent speckles","volume":"75","author":"Waterman-Storer","year":"1998","journal-title":"Biophys. J."},{"issue":"Suppl. 2","key":"10.1016\/B978-0-12-391857-4.00007-0_bb0205","doi-asserted-by":"crossref","first-page":"S225","DOI":"10.1096\/fasebj.13.9002.S225","article-title":"Fluorescent speckle microscopy of microtubules: How low can you go?","volume":"13","author":"Waterman-Storer","year":"1999","journal-title":"Faseb J."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0210","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1016\/S0960-9822(07)00515-5","article-title":"Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cells","volume":"8","author":"Waterman-Storer","year":"1998","journal-title":"Curr. Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0215","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1016\/j.cub.2005.03.019","article-title":"Efficient chromosome capture requires a bias in the \u2018search-and-capture\u2019 process during mitotic-spindle assembly","volume":"15","author":"Wollman","year":"2005","journal-title":"Curr. Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0220","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1038\/ncb1643","article-title":"Architectural dynamics of the meiotic spindle revealed by single-fluorophore imaging","volume":"9","author":"Yang","year":"2007","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0225","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1083\/jcb.200801105","article-title":"Regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindle","volume":"182","author":"Yang","year":"2008","journal-title":"J. Cell Biol."},{"key":"10.1016\/B978-0-12-391857-4.00007-0_bb0230","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1083\/jcb.131.3.721","article-title":"Kinetochore microtubule dynamics and the metaphase-anaphase transition","volume":"131","author":"Zhai","year":"1995","journal-title":"J. Cell Biol."}],"container-title":["Methods in Enzymology","Imaging and Spectroscopic Analysis of Living Cells - Optical and Spectroscopic Techniques"],"original-title":[],"deposited":{"date-parts":[[2021,12,26]],"date-time":"2021-12-26T10:20:31Z","timestamp":1640514031000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/B9780123918574000070"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":46,"URL":"https:\/\/doi.org\/10.1016\/b978-0-12-391857-4.00007-0","relation":{},"ISSN":["0076-6879"],"issn-type":[{"value":"0076-6879","type":"print"}],"subject":[],"published":{"date-parts":[[2012]]}}}