{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T05:48:49Z","timestamp":1788587329987,"version":"build-2803163510"},"reference-count":59,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,1,10]],"date-time":"2020-01-10T00:00:00Z","timestamp":1578614400000},"content-version":"vor","delay-in-days":374,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100014220","name":"Innovative Genomics Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100014220","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["K99GM118909"],"award-info":[{"award-number":["K99GM118909"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R00GM118909"],"award-info":[{"award-number":["R00GM118909"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"NIGMS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009559","name":"Life Sciences Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009559","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["1DP2EB018658-01"],"award-info":[{"award-number":["1DP2EB018658-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000070","name":"NIBIB","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000070","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["S10RR029668"],"award-info":[{"award-number":["S10RR029668"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["S10RR027303"],"award-info":[{"award-number":["S10RR027303"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell"],"published-print":{"date-parts":[[2019,1]]},"DOI":"10.1016\/j.cell.2018.11.052","type":"journal-article","created":{"date-parts":[[2019,1,10]],"date-time":"2019-01-10T16:56:16Z","timestamp":1547139376000},"page":"254-267.e16","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":95,"title":["CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification"],"prefix":"10.1016","volume":"176","author":[{"given":"Benjamin L.","family":"Oakes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christof","family":"Fellmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harneet","family":"Rishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kian L.","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn M.","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana C.","family":"Nadler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rayka","family":"Yokoo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam P.","family":"Arkin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer A.","family":"Doudna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David F.","family":"Savage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cell.2018.11.052_bib1","doi-asserted-by":"crossref","first-page":"2531","DOI":"10.1073\/pnas.0608779104","article-title":"Indirect activation of a plant nucleotide binding site-leucine-rich repeat protein by a bacterial protease","volume":"104","author":"Ade","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cell.2018.11.052_bib2","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1146\/annurev.phyto.42.040103.110731","article-title":"Type III secretion system effector proteins: double agents in bacterial disease and plant defense","volume":"42","author":"Alfano","year":"2004","journal-title":"Annu. Rev. Phytopathol."},{"key":"10.1016\/j.cell.2018.11.052_bib3","doi-asserted-by":"crossref","first-page":"R106","DOI":"10.1186\/gb-2010-11-10-r106","article-title":"Differential expression analysis for sequence count data","volume":"11","author":"Anders","year":"2010","journal-title":"Genome Biol."},{"key":"10.1016\/j.cell.2018.11.052_bib4","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1038\/nature13579","article-title":"Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease","volume":"513","author":"Anders","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.cell.2018.11.052_bib5","doi-asserted-by":"crossref","first-page":"15145","DOI":"10.1038\/nplants.2015.145","article-title":"Conferring resistance to geminiviruses with the CRISPR-Cas prokaryotic immune system","volume":"1","author":"Baltes","year":"2015","journal-title":"Nat. Plants"},{"key":"10.1016\/j.cell.2018.11.052_bib6","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1110\/ps.39001","article-title":"Random circular permutation leading to chain disruption within and near alpha helices in the catalytic chains of aspartate transcarbamoylase: effects on assembly, stability, and function","volume":"10","author":"Beernink","year":"2001","journal-title":"Protein Sci."},{"key":"10.1016\/j.cell.2018.11.052_bib7","doi-asserted-by":"crossref","first-page":"12883","DOI":"10.1074\/jbc.M611318200","article-title":"Functional characterization of cis and trans activity of the Flavivirus NS2B-NS3 protease","volume":"282","author":"Bera","year":"2007","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.cell.2018.11.052_bib8","doi-asserted-by":"crossref","first-page":"e168","DOI":"10.1093\/nar\/gku936","article-title":"Easy quantitative assessment of genome editing by sequence trace decomposition","volume":"42","author":"Brinkman","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.cell.2018.11.052_bib9","doi-asserted-by":"crossref","first-page":"3497","DOI":"10.1021\/bi900039e","article-title":"Structural and thermodynamic analysis of a conformationally strained circular permutant of barnase","volume":"48","author":"Butler","year":"2009","journal-title":"Biochemistry"},{"key":"10.1016\/j.cell.2018.11.052_bib10","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1038\/nature10348","article-title":"Ebola virus entry requires the cholesterol transporter Niemann-Pick C1","volume":"477","author":"Carette","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.cell.2018.11.052_bib11","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1186\/s13059-015-0829-4","article-title":"Boosting plant immunity with CRISPR\/Cas","volume":"16","author":"Chaparro-Garcia","year":"2015","journal-title":"Genome Biol."},{"key":"10.1016\/j.cell.2018.11.052_bib12","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1038\/nmeth.3312","article-title":"Highly efficient Cas9-mediated transcriptional programming","volume":"12","author":"Chavez","year":"2015","journal-title":"Nat. Methods"},{"key":"10.1016\/j.cell.2018.11.052_bib13","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1016\/j.cell.2013.12.001","article-title":"Dynamic imaging of genomic loci in living human cells by an optimized CRISPR\/Cas system","volume":"155","author":"Chen","year":"2013","journal-title":"Cell"},{"key":"10.1016\/j.cell.2018.11.052_bib14","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1073\/pnas.0409468102","article-title":"Molecular characterization of proteolytic cleavage sites of the Pseudomonas syringae effector AvrRpt2","volume":"102","author":"Chisholm","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cell.2018.11.052_bib15","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1126\/science.1231143","article-title":"Multiplex genome engineering using CRISPR\/Cas systems","volume":"339","author":"Cong","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.cell.2018.11.052_bib16","doi-asserted-by":"crossref","first-page":"e32110","DOI":"10.7554\/eLife.32110","article-title":"Functional genomics of lipid metabolism in the oleaginous yeast Rhodosporidium toruloides","volume":"7","author":"Coradetti","year":"2018","journal-title":"eLife"},{"key":"10.1016\/j.cell.2018.11.052_bib17","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1038\/nchembio.1793","article-title":"Small molecule-triggered Cas9 protein with improved genome-editing specificity","volume":"11","author":"Davis","year":"2015","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.cell.2018.11.052_bib18","doi-asserted-by":"crossref","first-page":"1704","DOI":"10.1016\/j.celrep.2013.11.020","article-title":"An optimized microRNA backbone for effective single-copy RNAi","volume":"5","author":"Fellmann","year":"2013","journal-title":"Cell Rep."},{"key":"10.1016\/j.cell.2018.11.052_bib19","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/nrd.2016.238","article-title":"Cornerstones of CRISPR-Cas in drug discovery and therapy","volume":"16","author":"Fellmann","year":"2017","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.cell.2018.11.052_bib20","doi-asserted-by":"crossref","first-page":"3702","DOI":"10.1128\/jvi.68.6.3702-3712.1994","article-title":"The protease of herpes simplex virus type 1 is essential for functional capsid formation and viral growth","volume":"68","author":"Gao","year":"1994","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.cell.2018.11.052_bib21","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1038\/nature24644","article-title":"Programmable base editing of A\u22c5T to G\u22c5C in genomic DNA without DNA cleavage","volume":"551","author":"Gaudelli","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.cell.2018.11.052_bib22","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/j.cell.2014.09.029","article-title":"Genome-scale CRISPR-mediated control of gene repression and activation","volume":"159","author":"Gilbert","year":"2014","journal-title":"Cell"},{"key":"10.1016\/j.cell.2018.11.052_bib23","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1038\/nbt.2909","article-title":"Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification","volume":"32","author":"Guilinger","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.cell.2018.11.052_bib24","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1016\/S0020-7519(03)00163-2","article-title":"Modulation of host immune responses by nematode cystatins","volume":"33","author":"Hartmann","year":"2003","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.cell.2018.11.052_bib25","doi-asserted-by":"crossref","first-page":"5642","DOI":"10.1021\/ja512664v","article-title":"Optical control of CRISPR\/Cas9 gene editing","volume":"137","author":"Hemphill","year":"2015","journal-title":"J.\u00a0Am. Chem. Soc."},{"key":"10.1016\/j.cell.2018.11.052_bib26","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1038\/nbt.3199","article-title":"Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers","volume":"33","author":"Hilton","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.cell.2018.11.052_bib27","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1126\/science.1225829","article-title":"A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity","volume":"337","author":"Jinek","year":"2012","journal-title":"Science"},{"key":"10.1016\/j.cell.2018.11.052_bib28","doi-asserted-by":"crossref","first-page":"e00471","DOI":"10.7554\/eLife.00471","article-title":"RNA-programmed genome editing in human cells","volume":"2","author":"Jinek","year":"2013","journal-title":"eLife"},{"key":"10.1016\/j.cell.2018.11.052_bib29","doi-asserted-by":"crossref","first-page":"5457","DOI":"10.1111\/j.1742-4658.2006.05536.x","article-title":"A ribonuclease zymogen activated by the NS3 protease of the hepatitis C virus","volume":"273","author":"Johnson","year":"2006","journal-title":"FEBS J."},{"key":"10.1016\/j.cell.2018.11.052_bib30","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1021\/acssynbio.5b00305","article-title":"The structure of a thermophilic kinase shapes fitness upon random circular permutation","volume":"5","author":"Jones","year":"2016","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.cell.2018.11.052_bib31","doi-asserted-by":"crossref","first-page":"11965","DOI":"10.1128\/JVI.01879-14","article-title":"Inactivation of the human papillomavirus E6 or E7 gene in cervical carcinoma cells by using a bacterial CRISPR\/Cas RNA-guided endonuclease","volume":"88","author":"Kennedy","year":"2014","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.cell.2018.11.052_bib32","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1126\/science.aad3436","article-title":"Using decoys to expand the recognition specificity of a plant disease resistance protein","volume":"351","author":"Kim","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.cell.2018.11.052_bib33","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1101\/gr.219089.116","article-title":"Genome surgery using Cas9 ribonucleoproteins for the treatment of age-related macular degeneration","volume":"27","author":"Kim","year":"2017","journal-title":"Genome Res."},{"key":"10.1016\/j.cell.2018.11.052_bib34","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1038\/nature17946","article-title":"Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage","volume":"533","author":"Komor","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.cell.2018.11.052_bib35","series-title":"Handbook of Proteolytic Enzymes","first-page":"3112","article-title":"Flavivirin","author":"K\u00fcmmerer","year":"2013"},{"key":"10.1016\/j.cell.2018.11.052_bib36","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1126\/science.1232033","article-title":"RNA-guided human genome engineering via Cas9","volume":"339","author":"Mali","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.cell.2018.11.052_bib37","doi-asserted-by":"crossref","first-page":"e71","DOI":"10.1093\/nar\/gks060","article-title":"A transposase strategy for creating libraries of circularly permuted proteins","volume":"40","author":"Mehta","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.cell.2018.11.052_bib38","article-title":"CRISPR-Cas9 interference in cassava linked to the evolution of editing-resistant geminiviruses","author":"Mehta","year":"2018","journal-title":"bioRxiv"},{"key":"10.1016\/j.cell.2018.11.052_bib39","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/B978-0-12-801185-0.00024-6","article-title":"Protein engineering of Cas9 for enhanced function","volume":"546","author":"Oakes","year":"2014","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.cell.2018.11.052_bib40","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1038\/nbt.3528","article-title":"Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch","volume":"34","author":"Oakes","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.cell.2018.11.052_bib41","doi-asserted-by":"crossref","first-page":"3313","DOI":"10.1038\/s41467-018-05641-3","article-title":"Extension of the crRNA enhances Cpf1 gene editing in\u00a0vitro and in\u00a0vivo","volume":"9","author":"Park","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.cell.2018.11.052_bib42","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/nbt.3804","article-title":"GuideScan software for improved single and paired CRISPR guide RNA design","volume":"35","author":"Perez","year":"2017","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.cell.2018.11.052_bib43","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1038\/nsb884","article-title":"Creation of a zymogen","volume":"10","author":"Plainkum","year":"2003","journal-title":"Nat. Struct. Biol."},{"key":"10.1016\/j.cell.2018.11.052_bib44","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1016\/j.cell.2013.02.022","article-title":"Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression","volume":"152","author":"Qi","year":"2013","journal-title":"Cell"},{"key":"10.1016\/j.cell.2018.11.052_bib45","doi-asserted-by":"crossref","first-page":"13466","DOI":"10.1021\/ja053932h","article-title":"Improving the catalytic activity of Candida antarctica lipase B by circular permutation","volume":"127","author":"Qian","year":"2005","journal-title":"J.\u00a0Am. Chem. Soc."},{"key":"10.1016\/j.cell.2018.11.052_bib46","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.virol.2005.08.043","article-title":"Host cell killing by the West Nile Virus NS2B-NS3 proteolytic complex: NS3 alone is sufficient to recruit caspase-8-based apoptotic pathway","volume":"345","author":"Ramanathan","year":"2006","journal-title":"Virology"},{"key":"10.1016\/j.cell.2018.11.052_bib47","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.copbio.2017.03.025","article-title":"Switchable Cas9","volume":"48","author":"Richter","year":"2017","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.cell.2018.11.052_bib48","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1016\/j.cell.2016.01.011","article-title":"Precision tumor recognition by T\u00a0cells with combinatorial antigen-sensing circuits","volume":"164","author":"Roybal","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.cell.2018.11.052_bib49","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1038\/nmeth.3047","article-title":"Improved vectors and genome-wide libraries for CRISPR screening","volume":"11","author":"Sanjana","year":"2014","journal-title":"Nat. Methods"},{"key":"10.1016\/j.cell.2018.11.052_bib50","series-title":"Handbook of Proteolytic Enzymes","first-page":"2427","article-title":"Potyvirus NIa protease","author":"Seon Han","year":"2013"},{"key":"10.1016\/j.cell.2018.11.052_bib51","series-title":"Handbook of Proteolytic Enzymes","first-page":"2396","article-title":"Picornain 3C","author":"Skern","year":"2013"},{"key":"10.1016\/j.cell.2018.11.052_bib52","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1038\/nbt.3806","article-title":"Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes","volume":"35","author":"Staahl","year":"2017","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.cell.2018.11.052_bib53","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.cell.2014.09.039","article-title":"A protein-tagging system for signal amplification in gene expression and fluorescence imaging","volume":"159","author":"Tanenbaum","year":"2014","journal-title":"Cell"},{"key":"10.1016\/j.cell.2018.11.052_bib54","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.1111\/mpp.12613","article-title":"Cassava brown streak disease: historical timeline, current knowledge and future prospects","volume":"19","author":"Tomlinson","year":"2018","journal-title":"Mol. Plant Pathol."},{"key":"10.1016\/j.cell.2018.11.052_bib55","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1038\/nbt.2908","article-title":"Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing","volume":"32","author":"Tsai","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.cell.2018.11.052_bib56","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1126\/science.aac7041","article-title":"Identification and characterization of essential genes in the human genome","volume":"350","author":"Wang","year":"2015","journal-title":"Science"},{"key":"10.1016\/j.cell.2018.11.052_bib57","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1093\/protein\/gzp034","article-title":"Tying up the loose ends: circular permutation decreases the proteolytic susceptibility of recombinant proteins","volume":"22","author":"Whitehead","year":"2009","journal-title":"Protein Eng. Des. Sel."},{"key":"10.1016\/j.cell.2018.11.052_bib58","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.tibtech.2010.10.004","article-title":"Circular permutation: a different way to engineer enzyme structure and function","volume":"29","author":"Yu","year":"2011","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.cell.2018.11.052_bib59","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1038\/nbt.3081","article-title":"Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in\u00a0vitro and in\u00a0vivo","volume":"33","author":"Zuris","year":"2015","journal-title":"Nat. Biotechnol."}],"container-title":["Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0092867418315836?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0092867418315836?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T11:36:41Z","timestamp":1758800201000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0092867418315836"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1]]},"references-count":59,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["S0092867418315836"],"URL":"https:\/\/doi.org\/10.1016\/j.cell.2018.11.052","relation":{},"ISSN":["0092-8674"],"issn-type":[{"value":"0092-8674","type":"print"}],"subject":[],"published":{"date-parts":[[2019,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification","name":"articletitle","label":"Article Title"},{"value":"Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cell.2018.11.052","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Inc.","name":"copyright","label":"Copyright"}]}}