{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T22:52:42Z","timestamp":1776120762280,"version":"3.50.1"},"reference-count":53,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["306060\/2022\\u20131"],"award-info":[{"award-number":["306060\/2022\\u20131"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Foundation","doi-asserted-by":"publisher","award":["NNF20SA0066233"],"award-info":[{"award-number":["NNF20SA0066233"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"State of Sao Paulo Research Foundation","doi-asserted-by":"publisher","award":["2022\/02456\\u20130"],"award-info":[{"award-number":["2022\/02456\\u20130"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"State of Sao Paulo Research Foundation","doi-asserted-by":"publisher","award":["2019\/03981\\u20139"],"award-info":[{"award-number":["2019\/03981\\u20139"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"State of Sao Paulo Research Foundation","doi-asserted-by":"publisher","award":["2017\/21819\\u20139"],"award-info":[{"award-number":["2017\/21819\\u20139"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Enzyme and Microbial Technology"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.enzmictec.2026.110836","type":"journal-article","created":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T17:06:24Z","timestamp":1772125584000},"page":"110836","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Tailored high-cell-density fed-batch cultivation and induction strategies for optimized production of a low-immunogenic L-asparaginase mutant in Escherichia coli"],"prefix":"10.1016","volume":"197","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1135-4827","authenticated-orcid":false,"given":"Larissa Pereira","family":"Brumano","sequence":"first","affiliation":[]},{"given":"Diego","family":"Grumbach","sequence":"additional","affiliation":[]},{"given":"Flaviana","family":"da Silva Chaves","sequence":"additional","affiliation":[]},{"given":"Gisele","family":"Monteiro","sequence":"additional","affiliation":[]},{"given":"Adalberto","family":"Pessoa Jr","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7182-6198","authenticated-orcid":false,"given":"Solange I.","family":"Mussatto","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"key":"10.1016\/j.enzmictec.2026.110836_bib1","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.ijbiomac.2017.12.165","article-title":"Glutaminase free l-asparaginase from Vibrio cholerae: heterologous expression, purification and biochemical characterization","volume":"111","author":"Radha","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib2","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.matpr.2022.05.227","article-title":"Fabrication and characterization of L-asparaginase entrapped polymeric nanoparticles for asparagine biosensor construction","volume":"67","author":"Singh","year":"2022","journal-title":"Mater. Today Proc."},{"key":"10.1016\/j.enzmictec.2026.110836_bib3","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1080\/10826068.2021.1931881","article-title":"L-asparaginase from Aspergillus oryzae spp.: effects of production process and biochemical parameters","volume":"52","author":"da Cunha","year":"2022","journal-title":"Prep. Biochem. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib4","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1042\/BJ20061708","article-title":"Tailoring structure\u2013function properties of L-asparaginase: engineering resistance to trypsin cleavage","volume":"404","author":"Kotzia","year":"2007","journal-title":"Biochem. J."},{"key":"10.1016\/j.enzmictec.2026.110836_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.lwt.2025.118304","article-title":"Refining L-asparaginase application for acrylamide reduction in sweet potatoes","volume":"231","author":"de Melo","year":"2025","journal-title":"LWT"},{"key":"10.1016\/j.enzmictec.2026.110836_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodchem.2025.145268","article-title":"Immobilization of L-asparaginase on genipin and divinyl sulfone cross-linked multi-walled carbon nanotubes and silica supports for acrylamide mitigation","volume":"491","author":"T\u00fclek","year":"2025","journal-title":"Food Chem."},{"key":"10.1016\/j.enzmictec.2026.110836_bib7","doi-asserted-by":"crossref","first-page":"1858","DOI":"10.1038\/sj.leu.2400834","article-title":"Cell cycle arrest and apoptosis of leukemia cells induced by L-asparaginase","volume":"11","author":"Ueno","year":"1997","journal-title":"Leukemia"},{"key":"10.1016\/j.enzmictec.2026.110836_bib8","first-page":"321","article-title":"Uber desamidierung im Tierkorper","volume":"5","author":"Lang","year":"1904","journal-title":"Beitr. Chem. Physiol. Pathol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib9","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1084\/jem.98.6.583","article-title":"Regression of transplanted lymphomas induced in vivo by means of normal guinea pig serum. II. Studies on the nature of the active serum constituent: histological mechanism of the regression: tests for effects of guinea pig serum on lymphoma cells in vitro","volume":"98","author":"Kidd","year":"1953","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.enzmictec.2026.110836_bib10","doi-asserted-by":"crossref","first-page":"62","DOI":"10.4103\/0976-500X.184769","article-title":"L-asparaginase in the treatment of patients with acute lymphoblastic leukemia","volume":"7","author":"Egler","year":"2016","journal-title":"J. Pharmacol. Pharmacother."},{"key":"10.1016\/j.enzmictec.2026.110836_bib11","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1001\/jama.1967.03130220070012","article-title":"L-asparaginase therapy for leukemia and other malignant neoplasms. Remission in human leukemia","volume":"202","author":"Hill","year":"1967","journal-title":"JAMA"},{"key":"10.1016\/j.enzmictec.2026.110836_bib12","unstructured":"World Health Organization, Web Annex BWorld Health Organization Model List of Essential Medicines for Children \u2013 9th List, 2023, in: Sel. Use Essent. Med. 2023 Exec. Summ. Rep. 24th WHO Expert Comm. Sel. Use Essent. Med. 24\u201328 April 2023, Geneva, 2023: p. 46. https:\/\/iris.who.int\/bitstream\/handle\/10665\/371091\/WHO-MHP-HPS-EML-2023.03-eng.pdf?sequence=1.."},{"key":"10.1016\/j.enzmictec.2026.110836_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2021.111616","article-title":"Circumventing the side effects of L-asparaginase","volume":"139","author":"Fonseca","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.enzmictec.2026.110836_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.bcp.2020.114230","article-title":"Influence of lysosomal protease sensitivity in the immunogenicity of the antitumor biopharmaceutical asparaginase","volume":"182","author":"Rodrigues","year":"2020","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib15","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2018.00212","article-title":"Development of L-asparaginase biobetters: current research status and review of the desirable quality profiles","volume":"6","author":"Brumano","year":"2019","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib16","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.procbio.2022.01.009","article-title":"Slow post-induction specific growth rate enhances recombinant protein expression in Escherichia coli: Pramlintide multimer and ranibizumab production as case studies","volume":"114","author":"Kumar","year":"2022","journal-title":"Process Biochem."},{"key":"10.1016\/j.enzmictec.2026.110836_bib17","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/0168-1656(94)00143-Z","article-title":"Simple fed-batch technique for high cell density cultivation of Escherichia coli","volume":"39","author":"Korz","year":"1995","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib18","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1007\/s002530051412","article-title":"High-cell-density cultivation of microorganisms","volume":"51","author":"Riesenberg","year":"1999","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib19","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1111\/j.1472-765X.2009.02694.x","article-title":"Effects of feeding and induction strategy on the production of Bm R1 antigen in recombinant E. coli","volume":"49","author":"Norsyahida","year":"2009","journal-title":"Lett. Appl. Microbiol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib20","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.bej.2008.04.013","article-title":"Induction strategies in fed-batch cultures for recombinant protein production in Escherichia coli: application to rhamnulose 1-phosphate aldolase","volume":"41","author":"Pinsach","year":"2008","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.enzmictec.2026.110836_bib21","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/1475-2859-11-58","article-title":"Direct measurements of IPTG enable analysis of the induction behavior of E. coli in high cell density cultures","volume":"11","author":"Fern\u00e1ndez-Castan\u00e9","year":"2012","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.enzmictec.2026.110836_bib22","series-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"2001"},{"key":"10.1016\/j.enzmictec.2026.110836_bib23","doi-asserted-by":"crossref","DOI":"10.1128\/aem.02311-23","article-title":"Plasmid-free cheater cells commonly evolve during laboratory growth","volume":"90","author":"Bedore","year":"2024","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib24","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/S0958-1669(05)80142-9","article-title":"High-cell-density cultivation of Escherichia coli","volume":"2","author":"Riesenberg","year":"1991","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib25","series-title":"Manual of Industrial Microbiology and Biotechnology","author":"Rinas","year":"2010"},{"key":"10.1016\/j.enzmictec.2026.110836_bib26","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/0167-7799(96)80930-9","article-title":"High cell-density culture of Escherichia coli","volume":"14","author":"Lee","year":"1996","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib27","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1099\/00221287-76-1-85","article-title":"Asparaginase and glutaminase activities of micro-organisms","volume":"76","author":"Imada","year":"1973","journal-title":"J. Gen. Microbiol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib28","article-title":"Novel site-specific PEGylated L-asparaginase","volume":"14","author":"Meneguetti","year":"2019","journal-title":"PLOS One"},{"key":"10.1016\/j.enzmictec.2026.110836_bib29","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.procbio.2022.11.024","article-title":"Recombinant production in Escherichia coli of a \u03b2-galactosidase fused to a cellulose-binding domain using low-cost inducers in fed-batch cultivation","volume":"124","author":"Gennari","year":"2023","journal-title":"Process Biochem."},{"key":"10.1016\/j.enzmictec.2026.110836_bib30","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1042\/BA20070089","article-title":"A proposed feeding strategy for the overproduction of recombinant proteins in Escherichia coli","volume":"49","author":"Babaeipour","year":"2008","journal-title":"Biotechnol. Appl. Biochem."},{"key":"10.1016\/j.enzmictec.2026.110836_bib31","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1038\/nbt1292-1550","article-title":"Recombinant protein expression in high cell density fed-batch cultures of Escherichia coli","volume":"10","author":"Yee","year":"1992","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib32","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s00449-003-0347-8","article-title":"High cell density fed-batch cultivation of Escherichia coli using exponential feeding combined with pH-stat","volume":"26","author":"Kim","year":"2004","journal-title":"Bioprocess Biosyst. Eng."},{"key":"10.1016\/j.enzmictec.2026.110836_bib33","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1002\/(SICI)1097-0290(19981105)60:3<271::AID-BIT1>3.0.CO;2-E","article-title":"Effect of post-induction nutrient feeding strategies on the production of bioadhesive protein in Escherichia coli","volume":"60","author":"Wong","year":"1998","journal-title":"Biotechnol. Bioeng"},{"key":"10.1016\/j.enzmictec.2026.110836_bib34","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/S1389-1723(99)80152-2","article-title":"Effect of growth rate reduction and genetic modifications on acetate accumulation and biomass yields in Escherichia coli","volume":"87","author":"Ponce","year":"1999","journal-title":"J. Biosci. Bioeng."},{"key":"10.1016\/j.enzmictec.2026.110836_bib35","doi-asserted-by":"crossref","first-page":"3952","DOI":"10.1128\/aem.60.11.3952-3958.1994","article-title":"Acetate metabolism by Escherichia coli in high-cell-density fermentation","volume":"60","author":"Kleman","year":"1994","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib36","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.ymben.2005.06.003","article-title":"Impact of dissolved oxygen concentration on acetate accumulation and physiology of E. coli BL21, evaluating transcription levels of key genes at different dissolved oxygen conditions","volume":"7","author":"Phue","year":"2005","journal-title":"Metab. Eng."},{"key":"10.1016\/j.enzmictec.2026.110836_bib37","doi-asserted-by":"crossref","first-page":"34951","DOI":"10.1021\/acsomega.4c04711","article-title":"Production process optimization of recombinant Erwinia carotovora L-asparaginase II in Escherichia coli fed-batch cultures and analysis of antileukemic potential","volume":"9","author":"Andrade","year":"2024","journal-title":"ACS Omega"},{"key":"10.1016\/j.enzmictec.2026.110836_bib38","doi-asserted-by":"crossref","first-page":"239","DOI":"10.3390\/fermentation11050239","article-title":"Optimized amino acid-enhanced medium for efficient l-asparaginase ii production in E. coli: from shake flask to bioreactor","volume":"11","author":"Lefin","year":"2025","journal-title":"Fermentation"},{"key":"10.1016\/j.enzmictec.2026.110836_bib39","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.biotechadv.2005.04.004","article-title":"Growing E. coli to high cell density\u2014a historical perspective on method development","volume":"23","author":"Shiloach","year":"2005","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.enzmictec.2026.110836_bib40","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1002\/jctb.401","article-title":"Effect of high shear stress on microbial viability","volume":"76","author":"Lange","year":"2001","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib41","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1021\/bp980059y","article-title":"Improvement of heterologous protein productivity by controlling postinduction specific growth rate in recombinant Escherichia coli under control of the PL promoter","volume":"14","author":"Lim","year":"1998","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.enzmictec.2026.110836_bib42","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/BF01569997","article-title":"Review: optimizing inducer and culture conditions for expression of foreign proteins under the control of thelac promoter","volume":"16","author":"Donovan","year":"1996","journal-title":"J. Ind. Microbiol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib43","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0022-2836(86)90385-2","article-title":"Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes","volume":"189","author":"Studier","year":"1986","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib44","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.bej.2012.10.006","article-title":"IPTG limitation avoids metabolic burden and acetic acid accumulation in induced fed-batch cultures of Escherichia coli M15 under glucose limiting conditions","volume":"70","author":"Lecina","year":"2013","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.enzmictec.2026.110836_bib45","doi-asserted-by":"crossref","first-page":"14","DOI":"10.3390\/pharmaceutics13010014","article-title":"Development of processes for recombinant L-asparaginase II production by Escherichia coli BL21 (De3): from shaker to bioreactors","volume":"13","author":"Barros","year":"2020","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.enzmictec.2026.110836_bib46","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.jbiotec.2011.12.007","article-title":"Evidencing the role of lactose permease in IPTG uptake by Escherichia coli in fed-batch high cell density cultures","volume":"157","author":"Fern\u00e1ndez-Castan\u00e9","year":"2012","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib47","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.mib.2016.07.009","article-title":"Overloaded and stressed: whole-cell considerations for bacterial synthetic biology","volume":"33","author":"Borkowski","year":"2016","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib48","doi-asserted-by":"crossref","DOI":"10.1093\/jimb\/kuad034","article-title":"Mechanistic aspects of IPTG (isopropylthio-\u03b2-galactoside) transport across the cytoplasmic membrane of Escherichia coli \u2014a rate limiting step in the induction of recombinant protein expression","volume":"50","author":"Simas","year":"2023","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib49","article-title":"Cunninghamella echinulata DSM1905 biofilm-based L-asparaginase production in pneumatically-driven bioreactors","volume":"19","author":"da","year":"2024","journal-title":"PLOS One"},{"key":"10.1016\/j.enzmictec.2026.110836_bib50","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2019.00016","article-title":"Fed-batch production of Saccharomyces cerevisiae L-asparaginase II by Recombinant Pichia pastoris MUTs strain","volume":"7","author":"Rodrigues","year":"2019","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.enzmictec.2026.110836_bib51","doi-asserted-by":"crossref","DOI":"10.1016\/j.bej.2021.108083","article-title":"Increased glycosylated L-asparaginase production through selection of Pichia pastoris platform and oxygen-methanol control in fed-batches","volume":"173","author":"de Almeida Parizotto","year":"2021","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.enzmictec.2026.110836_bib52","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1590\/S0104-66322013000200003","article-title":"Recombinant Erwinia carotovora L-asparaginase II production in Escherichia coli fed-batch cultures","volume":"30","author":"Roth","year":"2013","journal-title":"Braz. J. Chem. Eng."},{"key":"10.1016\/j.enzmictec.2026.110836_bib53","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s00253-004-1867-0","article-title":"Optimization of extracellular production of recombinant asparaginase in Escherichia coli in shake-flask and bioreactor","volume":"68","author":"Khushoo","year":"2005","journal-title":"Appl. Microbiol. Biotechnol."}],"container-title":["Enzyme and Microbial Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141022926000190?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141022926000190?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T22:03:21Z","timestamp":1776117801000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0141022926000190"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":53,"alternative-id":["S0141022926000190"],"URL":"https:\/\/doi.org\/10.1016\/j.enzmictec.2026.110836","relation":{},"ISSN":["0141-0229"],"issn-type":[{"value":"0141-0229","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Tailored high-cell-density fed-batch cultivation and induction strategies for optimized production of a low-immunogenic L-asparaginase mutant in Escherichia coli","name":"articletitle","label":"Article Title"},{"value":"Enzyme and Microbial Technology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.enzmictec.2026.110836","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"110836"}}