{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T21:42:06Z","timestamp":1773178926464,"version":"3.50.1"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T00:00:00Z","timestamp":1750377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universidade Cat\u00f3lica Portuguesa, Escola Superior de Biotecnologia"},{"DOI":"10.13039\/100004800","name":"California Air Resources Board","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004800","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100030764","name":"Universidade Cat\u00f3lica Portuguesa","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100030764","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/50016\/2020"],"award-info":[{"award-number":["UIDB\/50016\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UI \/ BD \/151388\/2021"],"award-info":[{"award-number":["UI \/ BD \/151388\/2021"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Cleaner Production"],"published-print":{"date-parts":[[2025,8]]},"DOI":"10.1016\/j.jclepro.2025.146039","type":"journal-article","created":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T05:40:51Z","timestamp":1751002851000},"page":"146039","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Dynamics and interrelationships between antibiotic resistance, organic micropollutants and bacterial communities in full-scale rural constructed wetlands"],"prefix":"10.1016","volume":"519","author":[{"given":"A. Margarida","family":"Teixeira","sequence":"first","affiliation":[]},{"given":"Diana","family":"Matos","sequence":"additional","affiliation":[]},{"given":"Norberta","family":"Coelho","sequence":"additional","affiliation":[]},{"given":"Lahiruni M.","family":"Halwatura","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8912-9836","authenticated-orcid":false,"given":"Ivone","family":"Vaz-Moreira","sequence":"additional","affiliation":[]},{"given":"Paula M.L.","family":"Castro","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6512-7713","authenticated-orcid":false,"given":"Diana S.","family":"Aga","sequence":"additional","affiliation":[]},{"given":"C\u00e9lia M.","family":"Manaia","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"key":"10.1016\/j.jclepro.2025.146039_bib1","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1126\/science.aba3432","article-title":"Using sewage for surveillance of antimicrobial resistance","volume":"6478","author":"Aarestrup","year":"2020","journal-title":"Science"},{"key":"10.1016\/j.jclepro.2025.146039_bib2","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1039\/C9EW00559E","article-title":"Assessing pharmaceutical removal and reduction in toxicity provided by advanced wastewater treatment systems","volume":"6","author":"Angeles","year":"2020","journal-title":"Environ. Sci. Water Res. Technol."},{"key":"10.1016\/j.jclepro.2025.146039_bib3","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1080\/09593330.2014.893025","article-title":"Coliform bacteria removal from sewage in constructed wetlands planted with Mentha aquatica","volume":"35","author":"Avelar","year":"2014","journal-title":"Environ. Technol."},{"key":"10.1016\/j.jclepro.2025.146039_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.142554","article-title":"New insights on the combined removal of antibiotics and ARGs in urban wastewater through the use of two configurations of vertical subsurface flow constructed wetlands","volume":"755","author":"\u00c1vila","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.jclepro.2025.146039_bib5","doi-asserted-by":"crossref","DOI":"10.3390\/su142214989","article-title":"Constructed wetlands as nature-based solutions for the removal of antibiotics: performance, microbial response, and emergence of antimicrobial resistance (AMR)","volume":"14","author":"Bai","year":"2022","journal-title":"Sustainability"},{"key":"10.1016\/j.jclepro.2025.146039_bib6","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1186\/s40168-018-0470-z","article-title":"Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2's q2-feature-classifier plugin","volume":"6","author":"Bokulich","year":"2018","journal-title":"Microbiome"},{"key":"10.1016\/j.jclepro.2025.146039_bib7","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.jclepro.2025.146039_bib8","doi-asserted-by":"crossref","first-page":"4481","DOI":"10.1128\/AEM.07878-11","article-title":"Simple absolute quantification method correcting for quantitative PCR efficiency variations for microbial community samples","volume":"78","author":"Brankatschk","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jclepro.2025.146039_bib9","doi-asserted-by":"crossref","first-page":"63","DOI":"10.2166\/wst.2007.522","article-title":"Twenty years experience with constructed wetland systems in Denmark \u2013 what did we learn?","volume":"56","author":"Brix","year":"2007","journal-title":"Water Sci. Technol."},{"key":"10.1016\/j.jclepro.2025.146039_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ecoleng.2015.03.001","article-title":"Constructed wetland with a polyculture of ornamental plants for wastewater treatment at a rural tourism facility","volume":"79","author":"Calheiros","year":"2015","journal-title":"Ecol. Eng."},{"key":"10.1016\/j.jclepro.2025.146039_bib11","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: high-Resolution sample inference from illumina amplicon data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat. Methods"},{"key":"10.1016\/j.jclepro.2025.146039_bib12","doi-asserted-by":"crossref","first-page":"2639","DOI":"10.1038\/ismej.2017.119","article-title":"Exact sequence variants should replace operational taxonomic units in marker-gene data analysis","volume":"11","author":"Callahan","year":"2017","journal-title":"ISME J."},{"key":"10.1016\/j.jclepro.2025.146039_bib13","series-title":"Nature-Based Solutions","year":"2024"},{"key":"10.1016\/j.jclepro.2025.146039_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.136776","article-title":"Floating treatment wetlands: a review and bibliometric analysis","volume":"714","author":"Colares","year":"2020","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.jclepro.2025.146039_bib15","doi-asserted-by":"crossref","first-page":"2515","DOI":"10.3390\/w11122515","article-title":"Removal of organic micropollutants in wastewater treated by activated sludge and constructed wetlands: a comparative study","volume":"11","author":"Contreras","year":"2019","journal-title":"Water"},{"key":"10.1016\/j.jclepro.2025.146039_bib16","unstructured":"(EU) Directive 91\/271\/EEC: Council Directive of 21 May 1991concerning Urban Waste Water Treatment."},{"key":"10.1016\/j.jclepro.2025.146039_bib18","series-title":"Directive of the European Parliament and of the Council Concerning Urban Wastewater Treatment (Recast)","year":"2022"},{"key":"10.1016\/j.jclepro.2025.146039_bib19","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.chemosphere.2017.01.027","article-title":"Occurrence and elimination of antibiotic resistance genes in a long-term operation integrated surface flow constructed wetland","volume":"173","author":"Fang","year":"2017","journal-title":"Chemosphere"},{"key":"10.1016\/j.jclepro.2025.146039_bib20","doi-asserted-by":"crossref","first-page":"11269","DOI":"10.1007\/s11356-019-04546-y","article-title":"Neighbor urban wastewater treatment plants display distinct profiles of bacterial community and antibiotic resistance genes","volume":"26","author":"Fernandes","year":"2019","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"10.1016\/j.jclepro.2025.146039_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2022.128933","article-title":"The balance between treatment efficiency and receptor quality determines wastewater impacts on the dissemination of antibiotic resistance","volume":"434","author":"Ferreira","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.jclepro.2025.146039_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2023.164707","article-title":"Broad-range extraction of highly polar to non-polar organic contaminants for inclusive target analysis and suspect screening of environmental samples","volume":"893","author":"Halwatura","year":"2023","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.jclepro.2025.146039_bib23","doi-asserted-by":"crossref","first-page":"114","DOI":"10.3390\/antibiotics11010114","article-title":"Performance efficiency of conventional treatment plants and constructed wetlands towards reduction of antibiotic resistance","volume":"11","author":"Hazra","year":"2023","journal-title":"Antibiotics"},{"key":"10.1016\/j.jclepro.2025.146039_bib24","series-title":"An\u00e1lise De Consumo De Medicamentos Em Meio Ambulat\u00f3rio","year":"2023"},{"key":"10.1016\/j.jclepro.2025.146039_bib25","series-title":"Water Quality \u2013 Enumeration of Escherichia coli and Coliform Bacteria","year":"2014"},{"key":"10.1016\/j.jclepro.2025.146039_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.jenvman.2021.111986","article-title":"Constructed wetland, an eco-technology for wastewater treatment: a review on types of wastewater treated and components of the technology (macrophyte, biolfilm and substrate)","volume":"283","author":"Kataki","year":"2021","journal-title":"J. Environ. Manag."},{"key":"10.1016\/j.jclepro.2025.146039_bib27","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1007\/s11356-022-21947-8","article-title":"Changes in the composition of bacterial communities and pathogen levels during wastewater treatment","volume":"30","author":"Li","year":"2023","journal-title":"Environ. Sci. Pollut. Res."},{"key":"10.1016\/j.jclepro.2025.146039_bib28","doi-asserted-by":"crossref","first-page":"8174","DOI":"10.1038\/s41598-020-65031-y","article-title":"Metagenomic analysis of an urban resistome before and after wastewater treatment","volume":"10","author":"Lira","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jclepro.2025.146039_bib29","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.jhazmat.2014.06.015","article-title":"Behavior of tetracycline and sulfamethazine with corresponding resistance genes from swine wastewater in pilot-scale constructed wetlands","volume":"278","author":"Liu","year":"2014","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.jclepro.2025.146039_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2022.157226","article-title":"Fate of resistome components and characteristics of microbial communities in constructed wetlands and their receiving river","volume":"844","author":"Liu","year":"2022","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.jclepro.2025.146039_bib31","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1080\/10643389.2022.2085956","article-title":"Framework for establishing regulatory guidelines to control antibiotic resistance in treated effluents","volume":"53","author":"Manaia","year":"2023","journal-title":"Crit. Rev. Environ. Sci. Technol."},{"key":"10.1016\/j.jclepro.2025.146039_bib32","doi-asserted-by":"crossref","first-page":"3232","DOI":"10.3390\/w14203232","article-title":"Constructed wetlands as a solution for sustainable sanitation: a comprehensive review on integrating climate change resilience and circular economy","volume":"14","author":"Masoud","year":"2022","journal-title":"Water"},{"key":"10.1016\/j.jclepro.2025.146039_bib49","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.envint.2018.05.040","article-title":"Bacterial lineages putatively associated with the dissemination of antibiotic resistance genes in a full-scale urban wastewater treatment plant","volume":"118","author":"Narciso-da-Rocha","year":"2018","journal-title":"Environ. Int."},{"key":"10.1016\/j.jclepro.2025.146039_bib33","doi-asserted-by":"crossref","first-page":"3123","DOI":"10.1093\/bioinformatics\/btu494","article-title":"STAMP: statistical analysis of taxonomic and functional profiles","volume":"30","author":"Parks","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.jclepro.2025.146039_bib34","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.aau9124","article-title":"Antibiotic resistance in European wastewater treatment plants mirrors the pattern of clinical antibiotic resistance prevalence","volume":"5","author":"P\u00e4rn\u00e4nen","year":"2019","journal-title":"Sci. Adv."},{"key":"10.1016\/j.jclepro.2025.146039_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2024.122038","article-title":"Nature-based solutions for antibiotics and antimicrobial resistance removal in tertiary wastewater treatment: microbiological composition and risk assessment","volume":"261","author":"Pastor-Lopez","year":"2024","journal-title":"Water Res."},{"key":"10.1016\/j.jclepro.2025.146039_bib36","unstructured":"Regulation (EU) 2019\/1021 of the European Parliament and the Council of 20 June 2019 on Persistent Organic Pollutants (Recast)."},{"key":"10.1016\/j.jclepro.2025.146039_bib17","unstructured":"Regulation (EU) 2020\/741 of the european parliament and of the council of 25 may 2020 on minimum requirements for water reuse, available online https:\/\/eur-lex.europa.eu\/legal-content\/PT\/TXT\/?uri=CELEX%3A32020R0741."},{"key":"10.1016\/j.jclepro.2025.146039_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.jece.2018.02.022","article-title":"Inter-laboratory calibration of quantitative analyses of antibiotic resistance genes","author":"Rocha","year":"2020","journal-title":"J. Environ. Chem. Eng."},{"key":"10.1016\/j.jclepro.2025.146039_bib38","series-title":"Antibiotics and Antibiotic Resistance Genes in Wastewater: Occurrence and Removal Technologies. Phd Thesis","year":"2020"},{"key":"10.1016\/j.jclepro.2025.146039_bib39","series-title":"Silva 138.1 Database","author":"Silva","year":"2024"},{"key":"10.1016\/j.jclepro.2025.146039_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2023.120761","article-title":"Candidate biomarkers of antibiotic resistance for the monitoring of wastewater and the downstream environment","volume":"247","author":"Teixeira","year":"2023","journal-title":"Water Res."},{"key":"10.1016\/j.jclepro.2025.146039_bib41","series-title":"Constructed Wetlands Manual. UN-HABITAT Water for Asian Cities Programme Nepal","year":"2008"},{"key":"10.1016\/j.jclepro.2025.146039_bib42","series-title":"Estimation of Domestic and Industrial Waste Emissions to European Waters in the 2010s, EUR 29451 EN","author":"Vigiak","year":"2018"},{"key":"10.1016\/j.jclepro.2025.146039_bib43","series-title":"Proceedings of Taal2007: the 12th World Lake Conference","first-page":"965","article-title":"Constructed wetlands for wastewater treatment: a review","author":"Vymazal","year":"2008"},{"key":"10.1016\/j.jclepro.2025.146039_bib50","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.3390\/land11091388","article-title":"Constructed wetland for sustainable and low-cost wastewater treatment: review article","volume":"11","author":"Waly","year":"2022","journal-title":"Land"},{"key":"10.1016\/j.jclepro.2025.146039_bib44","year":"2023"},{"key":"10.1016\/j.jclepro.2025.146039_bib45","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.scitotenv.2015.09.047","article-title":"Sanitation in constructed wetlands: a review on the removal of human pathogens and fecal indicators","volume":"541","author":"Wu","year":"2016","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.jclepro.2025.146039_bib46","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1038\/s43017-023-00395-z","article-title":"Constructed wetlands for pollution control","volume":"4","author":"Wu","year":"2023","journal-title":"Nat. Rev. Earth Environ."},{"key":"10.1016\/j.jclepro.2025.146039_bib47","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1016\/j.chemosphere.2014.02.040","article-title":"Occurrence of antibiotics and antibiotic resistance genes in a sewage treatment plant and its effluent-receiving river","volume":"119","author":"Xu","year":"2015","journal-title":"Chemosphere"},{"key":"10.1016\/j.jclepro.2025.146039_bib48","article-title":"Profiling sediment bacterial communities and the response to patterndriven variations of total nitrogen and phosphorus in long-term polyculture ponds","volume":"11","author":"Zhang","year":"2024","journal-title":"Front. Mar. Sci."}],"container-title":["Journal of Cleaner Production"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0959652625013897?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0959652625013897?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T11:35:18Z","timestamp":1762342518000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0959652625013897"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":50,"alternative-id":["S0959652625013897"],"URL":"https:\/\/doi.org\/10.1016\/j.jclepro.2025.146039","relation":{},"ISSN":["0959-6526"],"issn-type":[{"value":"0959-6526","type":"print"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Dynamics and interrelationships between antibiotic resistance, organic micropollutants and bacterial communities in full-scale rural constructed wetlands","name":"articletitle","label":"Article Title"},{"value":"Journal of Cleaner Production","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jclepro.2025.146039","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"146039"}}