{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T22:01:19Z","timestamp":1773352879524,"version":"3.50.1"},"reference-count":28,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T00:00:00Z","timestamp":1713312000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["methods-x.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["MethodsX"],"published-print":{"date-parts":[[2024,6]]},"DOI":"10.1016\/j.mex.2024.102726","type":"journal-article","created":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T15:49:06Z","timestamp":1713455346000},"page":"102726","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Advancing cortisol measurements in zebrafish: Analytical validation of a commercial ELISA kit for skin mucus cortisol analysis"],"prefix":"10.1016","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8423-2305","authenticated-orcid":false,"given":"Sara","family":"Jorge","sequence":"first","affiliation":[]},{"given":"Lu\u00eds","family":"F\u00e9lix","sequence":"additional","affiliation":[]},{"given":"Benjam\u00edn","family":"Costas","sequence":"additional","affiliation":[]},{"given":"Ana M.","family":"Valentim","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"issue":"6","key":"10.1016\/j.mex.2024.102726_bib0001","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.1016\/j.pnpbp.2010.10.010","article-title":"The use of the zebrafish model in stress research","volume":"35","author":"Steenbergen","year":"2011","journal-title":"Prog. Neuro-Psychopharmacol. Biol. Psychiatry"},{"key":"10.1016\/j.mex.2024.102726_bib0002","series-title":"The Rights and Wrongs of Zebrafish: Behavioral Phenotyping of Zebrafish","author":"Kalueff","year":"2017"},{"key":"10.1016\/j.mex.2024.102726_bib0003","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1146\/annurev-physiol-012110-142320","article-title":"Zebrafish in endocrine systems: recent advances and implications for human disease","volume":"73","author":"L\u00f6hr","year":"2011","journal-title":"Annu. Rev. Physiol."},{"issue":"1","key":"10.1016\/j.mex.2024.102726_bib0004","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/10253890.2020.1724948","article-title":"Understanding neurobehavioral effects of acute and chronic stress in zebrafish","volume":"24","author":"Demin","year":"2021","journal-title":"Stress"},{"issue":"7446","key":"10.1016\/j.mex.2024.102726_bib0005","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/nature12111","article-title":"The zebrafish reference genome sequence and its relationship to the human genome","volume":"496","author":"Howe","year":"2013","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.mex.2024.102726_bib0006","doi-asserted-by":"crossref","first-page":"e19","DOI":"10.1002\/cpet.19","article-title":"Zebrafish: development of a vertebrate model organism","volume":"16","author":"Meyers","year":"2018","journal-title":"Curr. Protocols Essent. Lab. Tech."},{"issue":"1","key":"10.1016\/j.mex.2024.102726_bib0007","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-022-10274-0","article-title":"Non-invasive sampling of water-borne hormones demonstrates individual consistency of the cortisol response to stress in laboratory zebrafish (Danio rerio)","volume":"12","author":"Midttun","year":"2022","journal-title":"Sci. Rep."},{"issue":"9\u201310","key":"10.1016\/j.mex.2024.102726_bib0008","first-page":"255","article-title":"Non-invasive monitoring of steroids in fishes","volume":"100","author":"Ellis","year":"2013","journal-title":"Vet. Med. Aust."},{"key":"10.1016\/j.mex.2024.102726_bib0009","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.fsi.2016.11.005","article-title":"Using skin mucus to evaluate stress in gilthead seabream (Sparus aurata L.)","volume":"59","author":"Guardiola","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.mex.2024.102726_bib0010","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.3389\/fmicb.2020.01621","article-title":"Cortisol-related signatures of stress in the fish microbiome","volume":"11","author":"Uren Webster","year":"2020","journal-title":"Front. Microbiol."},{"issue":"4","key":"10.1016\/j.mex.2024.102726_bib0011","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1007\/BF00042888","article-title":"Functions for fish mucus","volume":"4","author":"Shephard","year":"1994","journal-title":"Rev. Fish Biol. Fisheries"},{"key":"10.1016\/j.mex.2024.102726_bib0012","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.aquaculture.2014.09.027","article-title":"Effects of transportation stress and addition of salt to transport water on the skin mucosal homeostasis of rainbow trout (Oncorhynchus mykiss)","volume":"435","author":"Tacchi","year":"2015","journal-title":"Aquaculture"},{"issue":"2","key":"10.1016\/j.mex.2024.102726_bib0013","doi-asserted-by":"crossref","first-page":"e22","DOI":"10.4081\/ejh.2010.e22","article-title":"Monitoring stress in fish by applying image analysis to their skin mucous cells","volume":"54","author":"Vatsos","year":"2010","journal-title":"Eur. J. Histochem"},{"issue":"11","key":"10.1016\/j.mex.2024.102726_bib0014","doi-asserted-by":"crossref","first-page":"1786","DOI":"10.1038\/nprot.2010.140","article-title":"Measuring behavioral and endocrine responses to novelty stress in adult zebrafish","volume":"5","author":"Cachat","year":"2010","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.mex.2024.102726_bib0015","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.cbpa.2015.06.020","article-title":"Is gill cortisol concentration a good acute stress indicator in fish? A study in rainbow trout and zebrafish","volume":"188","author":"Gesto","year":"2015","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"issue":"1","key":"10.1016\/j.mex.2024.102726_bib0016","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1089\/zeb.2012.0792","article-title":"Noninvasive measurement of steroid hormones in zebrafish holding-water","volume":"10","author":"F\u00e9lix","year":"2013","journal-title":"Zebrafish"},{"issue":"3\u20134","key":"10.1016\/j.mex.2024.102726_bib0017","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0044-8486(93)90331-R","article-title":"Validation of a microtitre plate ELISA for measuring cortisol in fish and comparison of stress responses of rainbow trout (Oncorhynchus mykiss) and lake trout (Salvelinus namaycush)","volume":"117","author":"Barry","year":"1993","journal-title":"Aquaculture"},{"issue":"3","key":"10.1016\/j.mex.2024.102726_bib0018","doi-asserted-by":"crossref","first-page":"227","DOI":"10.4141\/cjas2012-045","article-title":"Minimally invasive sampling media and the measurement of corticosteroids as biomarkers of stress in animals","volume":"92","author":"Cook","year":"2012","journal-title":"Can. J. Anim. Sci."},{"key":"10.1016\/j.mex.2024.102726_bib0019","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/s10695-007-9150-9","article-title":"Validation, use, and disadvantages of enzyme-linked immunosorbent assay kits for detection of cortisol in channel catfish, largemouth bass, red pacu, and golden shiners","volume":"34","author":"Sink","year":"2008","journal-title":"Fish Physiol. Biochem."},{"key":"10.1016\/j.mex.2024.102726_bib0020","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.jembe.2017.12.001","article-title":"Development of a validation protocol of enzyme immunoassay kits used for the analysis of steroid hormones in fish plasma","volume":"499","author":"Metcalfe","year":"2018","journal-title":"J. Exp. Mar. Biol. Ecol."},{"issue":"23","key":"10.1016\/j.mex.2024.102726_bib0021","doi-asserted-by":"crossref","first-page":"2224","DOI":"10.1016\/j.jchromb.2008.09.030","article-title":"Key aspects of analytical method validation and linearity evaluation","volume":"877","author":"Araujo","year":"2009","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.mex.2024.102726_bib0022","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12915-018-0618-0","article-title":"Biological clock function is linked to proactive and reactive personality types","volume":"16","author":"Tudorache","year":"2018","journal-title":"BMC Biol."},{"key":"10.1016\/j.mex.2024.102726_bib0023","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ohx.2016.07.001","article-title":"Open source laboratory sample rotator mixer and shaker","volume":"1","author":"Dhankani","year":"2017","journal-title":"HardwareX"},{"issue":"6","key":"10.1016\/j.mex.2024.102726_bib0024","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.3390\/ani13061120","article-title":"Housing conditions affect adult zebrafish (Danio rerio) behavior but not their physiological status","volume":"13","author":"Jorge","year":"2023","journal-title":"Animals"},{"key":"10.1016\/j.mex.2024.102726_bib0025","series-title":"A Practical Approach to Biological Assay Validation, Hoofddorp","author":"Ederveen","year":"2010"},{"issue":"2","key":"10.1016\/j.mex.2024.102726_bib0026","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.jpba.2006.04.023","article-title":"Quantification of recombinant human parathyroid hormone (rhPTH (1-84)) in human plasma by immunoassay: commercial kit evaluation and validation to support pharmacokinetic studies","volume":"42","author":"Sukovaty","year":"2006","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.mex.2024.102726_bib0027","series-title":"Guidance Document On Analytical Quality Control and Method Validation On Procedures For Pesticide Residues and Analysis in Food and Feed","year":"2018"},{"issue":"7","key":"10.1016\/j.mex.2024.102726_bib0028","article-title":"Reporting animal research: explanation and elaboration for the ARRIVE guidelines 2.0","volume":"18","author":"Du Sert","year":"2020","journal-title":"PLoS Biol."}],"container-title":["MethodsX"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2215016124001791?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2215016124001791?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,1,20]],"date-time":"2025-01-20T15:29:23Z","timestamp":1737386963000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2215016124001791"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6]]},"references-count":28,"alternative-id":["S2215016124001791"],"URL":"https:\/\/doi.org\/10.1016\/j.mex.2024.102726","relation":{},"ISSN":["2215-0161"],"issn-type":[{"value":"2215-0161","type":"print"}],"subject":[],"published":{"date-parts":[[2024,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Advancing cortisol measurements in zebrafish: Analytical validation of a commercial ELISA kit for skin mucus cortisol analysis","name":"articletitle","label":"Article Title"},{"value":"MethodsX","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mex.2024.102726","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"102726"}}