{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T04:24:58Z","timestamp":1773807898190,"version":"3.50.1"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:00:00Z","timestamp":1760659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014181","name":"Extremadura Government","doi-asserted-by":"publisher","award":["IB20078"],"award-info":[{"award-number":["IB20078"]}],"id":[{"id":"10.13039\/501100014181","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Theriogenology"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.theriogenology.2025.117710","type":"journal-article","created":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T19:52:53Z","timestamp":1760730773000},"page":"117710","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Presenilin-1 is present in boar spermatozoa and regulates acrosome reaction in the absence of glucose"],"prefix":"10.1016","volume":"251","author":[{"given":"David","family":"Mart\u00edn-Hidalgo","sequence":"first","affiliation":[]},{"given":"Beatriz","family":"Mac\u00edas-Garc\u00eda","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5568-548X","authenticated-orcid":false,"given":"Lauro","family":"Gonz\u00e1lez-Fern\u00e1ndez","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"key":"10.1016\/j.theriogenology.2025.117710_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1262\/jrd.2016-108","article-title":"Male infertility-related molecules involved in sperm-oocyte fusion","volume":"63","author":"Mou","year":"2017","journal-title":"J Reprod Dev"},{"key":"10.1016\/j.theriogenology.2025.117710_bib2","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23073929","article-title":"The role of sperm proteins IZUMO1 and TMEM95 in mammalian fertilization: a systematic review","volume":"23","author":"Hernandez-Falco","year":"2022","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.theriogenology.2025.117710_bib3","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2021.662032","article-title":"Oolemma receptors in mammalian molecular fertilization: function and new methods of study","volume":"9","author":"Jimenez-Movilla","year":"2021","journal-title":"Front Cell Dev Biol"},{"key":"10.1016\/j.theriogenology.2025.117710_bib4","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/978-3-319-30567-7_3","article-title":"Role of ion channels in the sperm acrosome reaction","volume":"220","author":"Beltran","year":"2016","journal-title":"Adv Anat Embryol Cell Biol"},{"key":"10.1016\/j.theriogenology.2025.117710_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.biosystems.2021.104524","article-title":"Role of calcium oscillations in sperm physiology","volume":"209","author":"Mata-Martinez","year":"2021","journal-title":"Biosystems"},{"key":"10.1016\/j.theriogenology.2025.117710_bib6","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.ydbio.2016.02.006","article-title":"Mouse sperm begin to undergo acrosomal exocytosis in the upper isthmus of the oviduct","volume":"411","author":"La Spina","year":"2016","journal-title":"Dev Biol"},{"key":"10.1016\/j.theriogenology.2025.117710_bib7","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1146\/annurev-cellbio-120219-021751","article-title":"The fertilization enigma: how sperm and egg fuse","volume":"37","author":"Deneke","year":"2021","journal-title":"Annu Rev Cell Dev Biol"},{"key":"10.1016\/j.theriogenology.2025.117710_bib8","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1038\/nature13203","article-title":"Juno is the egg Izumo receptor and is essential for mammalian fertilization","volume":"508","author":"Bianchi","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.theriogenology.2025.117710_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.anireprosci.2020.106513","article-title":"Boar spermatozoa proteomic profile varies in sperm collected during the summer and winter","volume":"219","author":"Martin-Hidalgo","year":"2020","journal-title":"Anim Reprod Sci"},{"key":"10.1016\/j.theriogenology.2025.117710_bib10","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a006304","article-title":"Presenilins and gamma-secretase: structure, function, and role in Alzheimer Disease","volume":"2","author":"De Strooper","year":"2012","journal-title":"Cold Spring Harb Perspect Med"},{"key":"10.1016\/j.theriogenology.2025.117710_bib11","article-title":"Presenilin: a multi-functional molecule in the pathogenesis of Alzheimer's Disease and other neurodegenerative diseases","volume":"25","author":"Sun","year":"2024","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.theriogenology.2025.117710_bib12","doi-asserted-by":"crossref","first-page":"1983","DOI":"10.1016\/j.biocel.2012.07.019","article-title":"Presenilins: role in calcium homeostasis","volume":"44","author":"Honarnejad","year":"2012","journal-title":"Int J Biochem Cell Biol"},{"key":"10.1016\/j.theriogenology.2025.117710_bib13","doi-asserted-by":"crossref","first-page":"261","DOI":"10.3233\/JAD-2010-1360","article-title":"Presenilin-1 holoprotein is an interacting partner of sarco endoplasmic reticulum calcium-ATPase and confers resistance to endoplasmic reticulum stress","volume":"20","author":"Jin","year":"2010","journal-title":"J Alzheimers Dis"},{"key":"10.1016\/j.theriogenology.2025.117710_bib14","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1016\/j.cell.2006.06.059","article-title":"Presenilins form ER Ca2+ leak channels, a function disrupted by familial Alzheimer's disease-linked mutations","volume":"126","author":"Tu","year":"2006","journal-title":"Cell"},{"key":"10.1016\/j.theriogenology.2025.117710_bib15","doi-asserted-by":"crossref","DOI":"10.1155\/2019\/7284967","article-title":"Presenilin 1 regulates [Ca(2+)]i and Mitochondria\/ER interaction in cultured rat hippocampal neurons","volume":"2019","author":"Korkotian","year":"2019","journal-title":"Oxid Med Cell Longev"},{"key":"10.1016\/j.theriogenology.2025.117710_bib16","doi-asserted-by":"crossref","first-page":"2381","DOI":"10.1073\/pnas.041603398","article-title":"Presenilin-1 binds cytoplasmic epithelial cadherin, inhibits cadherin\/p120 association, and regulates stability and function of the cadherin\/catenin adhesion complex","volume":"98","author":"Baki","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.theriogenology.2025.117710_bib17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/excr.2000.5098","article-title":"The role of Alzheimer's disease-related presenilin 1 in intercellular adhesion","volume":"263","author":"Singh","year":"2001","journal-title":"Exp Cell Res"},{"key":"10.1016\/j.theriogenology.2025.117710_bib18","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1186\/1471-213X-8-44","article-title":"A novel function for the presenilin family member spe-4: inhibition of spermatid activation in Caenorhabditis elegans","volume":"8","author":"Gosney","year":"2008","journal-title":"BMC Dev Biol"},{"key":"10.1016\/j.theriogenology.2025.117710_bib19","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.theriogenology.2024.04.006","article-title":"Induction of acrosome reaction by 4-Br-A23187 alters the glycoproteomic profile of boar spermatozoa","volume":"223","author":"Martin-Hidalgo","year":"2024","journal-title":"Theriogenology"},{"key":"10.1016\/j.theriogenology.2025.117710_bib20","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1530\/REP-22-0299","article-title":"The CASR antagonist NPS2143 induces loss of acrosomal integrity and proteolysis of SPACA1 in boar spermatozoa","volume":"165","author":"Macias-Garcia","year":"2023","journal-title":"Reproduction"},{"key":"10.1016\/j.theriogenology.2025.117710_bib21","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1095\/biolreprod.112.107078","article-title":"Focal adhesion kinases and calcium\/calmodulin-dependent protein kinases regulate protein tyrosine phosphorylation in stallion sperm","volume":"88","author":"Gonzalez-Fernandez","year":"2013","journal-title":"Biol Reprod"},{"key":"10.1016\/j.theriogenology.2025.117710_bib22","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1262\/jrd.2021-075","article-title":"Influence of different cellular concentrations of boar sperm suspensions on the induction of capacitation and acrosome reaction","volume":"68","author":"Martin-Hidalgo","year":"2022","journal-title":"J Reprod Dev"},{"key":"10.1016\/j.theriogenology.2025.117710_bib23","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0896-6273(00)80291-3","article-title":"Endoproteolysis of presenilin 1 and accumulation of processed derivatives in vivo","volume":"17","author":"Thinakaran","year":"1996","journal-title":"Neuron"},{"key":"10.1016\/j.theriogenology.2025.117710_bib24","doi-asserted-by":"crossref","first-page":"6299","DOI":"10.1038\/s41467-022-33817-5","article-title":"Molecular basis for isoform-selective inhibition of presenilin-1 by MRK-560","volume":"13","author":"Guo","year":"2022","journal-title":"Nat Commun"},{"key":"10.1016\/j.theriogenology.2025.117710_bib25","article-title":"Glucose prevents the acquisition of the capacitated state in pig spermatozoa","author":"Serrano","year":"2024","journal-title":"Andrology"},{"key":"10.1016\/j.theriogenology.2025.117710_bib26","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1002\/mrd.20099","article-title":"A unique mechanism for cyclic adenosine 3',5'-monophosphate-induced increase of 32-kDa tyrosine-phosphorylated protein in boar spermatozoa","volume":"69","author":"Harayama","year":"2004","journal-title":"Mol Reprod Dev"},{"key":"10.1016\/j.theriogenology.2025.117710_bib27","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2021.682790","article-title":"Discrete dynamic model of the mammalian sperm acrosome reaction: the influence of acrosomal pH and physiological heterogeneity","volume":"12","author":"Aldana","year":"2021","journal-title":"Front Physiol"},{"key":"10.1016\/j.theriogenology.2025.117710_bib28","doi-asserted-by":"crossref","DOI":"10.1002\/mrd.23772","article-title":"Roldan ERS. Sperm metabolism","volume":"91","author":"Abruzzese","year":"2024","journal-title":"Mol Reprod Dev"},{"key":"10.1016\/j.theriogenology.2025.117710_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.ceca.2021.102468","article-title":"Presenilin 1 is a direct regulator of the cardiac sarco\/endoplasmic reticulum calcium pump","volume":"99","author":"Bovo","year":"2021","journal-title":"Cell Calcium"},{"key":"10.1016\/j.theriogenology.2025.117710_bib30","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.ceca.2006.03.002","article-title":"Presenilin mutations linked to familial Alzheimer's disease reduce endoplasmic reticulum and Golgi apparatus calcium levels","volume":"39","author":"Zatti","year":"2006","journal-title":"Cell Calcium"},{"key":"10.1016\/j.theriogenology.2025.117710_bib31","doi-asserted-by":"crossref","DOI":"10.1126\/scisignal.2000818","article-title":"Gain-of-function enhancement of IP3 receptor modal gating by familial Alzheimer's disease-linked presenilin mutants in human cells and mouse neurons","volume":"3","author":"Cheung","year":"2010","journal-title":"Sci Signal"},{"key":"10.1016\/j.theriogenology.2025.117710_bib32","doi-asserted-by":"crossref","first-page":"192","DOI":"10.4103\/aja.aja_44_19","article-title":"Participation of the inositol 1,4,5-trisphosphate-gated calcium channel in the zona pellucida- and progesterone-induced acrosome reaction and calcium influx in human spermatozoa","volume":"22","author":"Li","year":"2020","journal-title":"Asian J Androl"},{"key":"10.1016\/j.theriogenology.2025.117710_bib33","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1530\/jrf.0.0960699","article-title":"Concentrations of energy substrates in oviductal fluid and blood plasma of pigs during the peri-ovulatory period","volume":"96","author":"Nichol","year":"1992","journal-title":"J Reprod Fertil"}],"container-title":["Theriogenology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0093691X25004364?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0093691X25004364?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T03:26:21Z","timestamp":1773804381000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0093691X25004364"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":33,"alternative-id":["S0093691X25004364"],"URL":"https:\/\/doi.org\/10.1016\/j.theriogenology.2025.117710","relation":{},"ISSN":["0093-691X"],"issn-type":[{"value":"0093-691X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Presenilin-1 is present in boar spermatozoa and regulates acrosome reaction in the absence of glucose","name":"articletitle","label":"Article Title"},{"value":"Theriogenology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.theriogenology.2025.117710","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"117710"}}