{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T18:16:34Z","timestamp":1775585794028,"version":"3.50.1"},"reference-count":94,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003958","name":"Technology Foundation STW","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003958","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bmn.com","cell.com","clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Trends in Biotechnology"],"published-print":{"date-parts":[[2025,7]]},"DOI":"10.1016\/j.tibtech.2025.04.004","type":"journal-article","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T11:46:33Z","timestamp":1746791193000},"page":"1690-1713","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Engineered thermoswitch in the baculovirus expression vector system for production of virus-like particle vaccines with minimized baculovirus contaminants"],"prefix":"10.1016","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4346-7156","authenticated-orcid":false,"given":"Linda","family":"van Oosten","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9292-5339","authenticated-orcid":false,"given":"Jort J.","family":"Altenburg","sequence":"additional","affiliation":[]},{"given":"Gwen","family":"Nowee","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3863-2427","authenticated-orcid":false,"given":"Dennis","family":"Kenbeek","sequence":"additional","affiliation":[]},{"given":"Tessa","family":"Neef","sequence":"additional","affiliation":[]},{"given":"Thomas","family":"Rouw","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9669-9439","authenticated-orcid":false,"given":"Vivian I.P.","family":"Tegelbeckers","sequence":"additional","affiliation":[]},{"given":"Jans","family":"van der Heijden","sequence":"additional","affiliation":[]},{"given":"Saskia","family":"Mentink","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0049-4126","authenticated-orcid":false,"given":"Wessel","family":"Willemsen","sequence":"additional","affiliation":[]},{"given":"Christina S.","family":"Hausjell","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7245-3031","authenticated-orcid":false,"given":"Louis","family":"Kuijpers","sequence":"additional","affiliation":[]},{"given":"Leo","family":"van der Pol","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0414-4899","authenticated-orcid":false,"given":"Ant\u00f3nio","family":"Rold\u00e3o","sequence":"additional","affiliation":[]},{"given":"Ricardo","family":"Correia","sequence":"additional","affiliation":[]},{"given":"Erwin","family":"van den Born","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7630-4295","authenticated-orcid":false,"given":"Ren\u00e9 H.","family":"Wijffels","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5662-0466","authenticated-orcid":false,"given":"Dirk E.","family":"Martens","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6664-4445","authenticated-orcid":false,"given":"Monique M.","family":"van Oers","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9301-0408","authenticated-orcid":false,"given":"Gorben P.","family":"Pijlman","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"key":"10.1016\/j.tibtech.2025.04.004_bb0005","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1038\/s41423-022-00897-8","article-title":"Virus-like particle vaccinology, from bench to bedside","volume":"19","author":"Mohsen","year":"2022","journal-title":"Cell. Mol. Immunol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0010","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.tibtech.2013.09.002","article-title":"Virus-like particle-based human vaccines: quality assessment based on structural and functional properties","volume":"31","author":"Zhao","year":"2013","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0015","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/j.it.2022.07.011","article-title":"Single-dose HPV vaccine immunity: is there a role for non-neutralizing antibodies?","volume":"43","author":"Quang","year":"2022","journal-title":"Trends Immunol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0020","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2022.994743","article-title":"Genetic engineering of baculovirus-insect cell system to improve protein production","volume":"10","author":"Hong","year":"2022","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0025","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.3390\/vaccines11071218","article-title":"Application of baculovirus expression vector system (BEVS) in vaccine development","volume":"11","author":"Hong","year":"2023","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0030","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1099\/vir.0.067108-0","article-title":"Thirty years of baculovirus-insect cell protein expression: from dark horse to mainstream technology","volume":"96","author":"van Oers","year":"2015","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0035","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1177\/153567600200700312","article-title":"Innovations\u2014biotechnology: baculovirus vectors as gene transfer vectors for mammalian cells: biosafety considerations","volume":"7","author":"Kost","year":"2002","journal-title":"Appl. Biosaf."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0040","doi-asserted-by":"crossref","first-page":"177","DOI":"10.2174\/1566523220999201217155945","article-title":"Evaluation of the nucleopolyhedrovirus of Anticarsia gemmatalis as a vector for gene therapy in mammals","volume":"21","author":"Parsza","year":"2020","journal-title":"Curr. Gene Ther."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0045","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.3390\/genes11111306","article-title":"Unraveling the genome-wide impact of recombinant baculovirus infection in mammalian cells for gene delivery","volume":"11","author":"Shin","year":"2020","journal-title":"Genes (Basel)"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0050","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/0042-6822(83)90067-3","article-title":"Autographa californica nuclear polyhedrosis virus (AcNPV) DNA does not persist in mass cultures of mammalian cells","volume":"125","author":"Via","year":"1983","journal-title":"Virology"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0055","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/imm.13607","article-title":"TLR9 activation is required for cytotoxic response elicited by baculovirus capsid display","volume":"169","author":"Molinari","year":"2022","journal-title":"Immunology"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0060","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1128\/JVI.75.2.903-909.2001","article-title":"Chromosomal integration of transduced recombinant baculovirus DNA in mammalian cells","volume":"75","author":"Merrihew","year":"2001","journal-title":"J. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0065","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1128\/JVI.79.5.2847-2858.2005","article-title":"Involvement of the Toll-like receptor 9 signaling pathway in the induction of innate immunity by baculovirus","volume":"79","author":"Abe","year":"2005","journal-title":"J. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0070","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0024108","article-title":"Baculovirus capsid display potentiates OVA cytotoxic and innate immune responses","volume":"6","author":"Molinari","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0075","series-title":"ICH topic Q5A (R1) Quality of Biotechnological Products: Viral Safety Evaluation of Biotechnology Products Derived from Cell Lines of Human or Animal Origin","author":"European Medicines Agency","year":"1997"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0080","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.pep.2013.05.009","article-title":"Use of baculovirus expression system for generation of virus-like particles: successes and challenges","volume":"90","author":"Liu","year":"2013","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0085","doi-asserted-by":"crossref","first-page":"S42","DOI":"10.1016\/j.jip.2011.05.004","article-title":"Large-scale production and purification of VLP-based vaccines","volume":"107","author":"Vicente","year":"2011","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0090","doi-asserted-by":"crossref","first-page":"407","DOI":"10.4161\/hv.6.5.11023","article-title":"Molecular and structural characterization of the L1 virus-like particles that are used as vaccine antigens in Cervarix TM, the AS04-adjuvanted HPV-16 and -18 cervical cancer vaccine","volume":"6","author":"Deschuyteneer","year":"2010","journal-title":"Hum. Vaccin."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0095","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2023.1166430","article-title":"Editorial: Advances in bioprocessing of viral vectors and virus-like particles","volume":"11","author":"Vormittag","year":"2023","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0100","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00038-17","article-title":"Beta-propiolactone inactivation of coxsackievirus A16 induces structural alteration and surface modification of viral capsids","volume":"91","author":"Fan","year":"2017","journal-title":"J. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0105","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004167","article-title":"Formalin inactivation of Japanese encephalitis virus vaccine alters the antigenicity and immunogenicity of a neutralization epitope in envelope protein domain III","volume":"9","author":"Fan","year":"2015","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0110","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/S0264-410X(00)00259-0","article-title":"Effect of different baculovirus inactivation procedures on the integrity and immunogenicity of porcine parvovirus-like particles","volume":"19","author":"Rueda","year":"2000","journal-title":"Vaccine"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0115","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1586\/erv.12.153","article-title":"Insect cells as a production platform of complex virus-like particles","volume":"12","author":"Fernandes","year":"2013","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0120","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.nbt.2017.07.010","article-title":"Production of virus-like particles for vaccines","volume":"39","author":"Fuenmayor","year":"2017","journal-title":"New Biotechnol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0125","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1123805","article-title":"Platforms, advances, and technical challenges in virus-like particles-based vaccines","volume":"14","author":"Gupta","year":"2023","journal-title":"Front. Immunol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0130","doi-asserted-by":"crossref","first-page":"2376","DOI":"10.1002\/jctb.6821","article-title":"Current status and future challenges in transitioning to continuous bioprocessing of virus-like particles","volume":"97","author":"Mittal","year":"2022","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0135","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0002124","article-title":"Effective chikungunya virus-like particle vaccine produced in insect cells","volume":"7","author":"Metz","year":"2013","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0140","article-title":"Enhanced Zika virus-like particle development using Baculovirus spp. constructs","volume":"95","author":"Malogolovkin","year":"2022","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0145","doi-asserted-by":"crossref","first-page":"2104","DOI":"10.1086\/425933","article-title":"Induction of sterilizing immunity against West Nile virus (WNV), by immunization with WNV-like particles produced in insect cells","volume":"190","author":"Qiao","year":"2004","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0150","doi-asserted-by":"crossref","first-page":"7367","DOI":"10.1007\/s00253-019-10038-3","article-title":"Production of HIV-1-based virus-like particles for vaccination: achievements and limits","volume":"103","author":"Cervera","year":"2019","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0155","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.3390\/vaccines9121504","article-title":"Innovations in the insect cell expression system for industrial recombinant vaccine antigen production","volume":"9","author":"Cox","year":"2021","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0160","doi-asserted-by":"crossref","first-page":"223","DOI":"10.3390\/v12020223","article-title":"Quality assessment of virus-like particles at single particle level: a comparative study","volume":"12","author":"Gonz\u00e1lez-Dom\u00ednguez","year":"2020","journal-title":"Viruses"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0165","unstructured":"Merten, O-W. et al. (2012) Genethon. Baculovirus-based production of biopharmaceuticals free of contaminating baculovirual virions, US 2012\/0149010 A1"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0170","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1002\/bit.23028","article-title":"Engineering of baculovirus vectors for the manufacture of virion-free biopharmaceuticals","volume":"108","author":"Marek","year":"2011","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0175","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1099\/jgv.0.001002","article-title":"Production of GP64-free virus-like particles from baculovirus-infected insect cells","volume":"99","author":"Chaves","year":"2018","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0180","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.jbiotec.2023.01.013","article-title":"A lef5-deficient baculovirus expression system with no virion contamination and promoting secretion","volume":"365","author":"Hu","year":"2023","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0185","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.1099\/vir.0.19438-0","article-title":"Spontaneous excision of BAC vector sequences from bacmid-derived baculovirus expression vectors upon passage in insect cells","volume":"84","author":"Pijlman","year":"2003","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0190","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1111\/1748-5967.12500","article-title":"The influence of serial passage on the stability of an exogenous gene expression in recombinant baculovirus","volume":"51","author":"Wu","year":"2021","journal-title":"Entomol. Res."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0195","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s00253-002-0976-x","article-title":"Large scale production and downstream processing of a recombinant porcine parvovirus vaccine","volume":"59","author":"Maranga","year":"2002","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0200","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1128\/jvi.31.1.190-198.1979","article-title":"Genetic analysis of a baculovirus, Autographa californica nuclear polyhedrosis virus I. Isolation of temperature-sensitive mutants and assortment into complementation groups","volume":"31","author":"Brown","year":"1979","journal-title":"J. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0205","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1099\/0022-1317-62-2-369","article-title":"Bromodeoxyuridine-induced mutants of Autographa californica nuclear polyhedrosis virus defective in occlusion body formation","volume":"62","author":"Duncan","year":"1982","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0210","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1128\/jvi.31.1.240-252.1979","article-title":"Isolation, complementation, and initial characterization of temperature-sensitive mutants of the baculovirus Autographa californica nuclear polyhedrosis virus","volume":"31","author":"Lee","year":"1979","journal-title":"J. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0215","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1128\/jvi.39.3.973-976.1981","article-title":"Construction of a genetic map of the baculovirus Autographa californica nuclear polyhedrosis virus by marker rescue of temperature-sensitive mutants","volume":"39","author":"Miller","year":"1981","journal-title":"J. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0220","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/0042-6822(90)90189-X","article-title":"Isolation of temperature sensitive mutants of Autographa californica nuclear polyhedrosis virus: phenotype characterization of baculovirus mutants defective in very late gene expression","volume":"175","author":"Partington","year":"1990","journal-title":"Virology"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0225","series-title":"Baculovirus Molecular Biology","first-page":"1","article-title":"The AcMNPV genome: gene content, conservation, and function","author":"Rohrmann","year":"2019"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0230","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1006\/viro.1997.8612","article-title":"A role for baculovirus GP41 in budded virus production","volume":"233","author":"Olszewski","year":"1997","journal-title":"Virology"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0235","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.02083-17","article-title":"The functional oligomeric state of tegument protein GP41 is essential for baculovirus budded virion and occlusion-derived virion assembly","volume":"92","author":"Li","year":"2018","journal-title":"J. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0240","doi-asserted-by":"crossref","first-page":"3324","DOI":"10.1128\/jvi.66.6.3324-3329.1992","article-title":"A structural polypeptide of the baculovirus Autographa californica nuclear polyhedrosis virus contains O-linked N-acetylglucosamine","volume":"66","author":"Whitford","year":"1992","journal-title":"J. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0245","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0250","article-title":"Real-time online monitoring of insect cell proliferation and baculovirus infection using digital differential holographic microscopy and machine learning","volume":"39","author":"Altenburg","year":"2022","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0255","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.jbiotec.2019.10.004","article-title":"Adaptive laboratory evolution of stable insect cell lines for improved HIV-Gag VLPs production","volume":"307","author":"Fernandes","year":"2020","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0260","first-page":"109","article-title":"Scarless baculovirus genome editing using lambda-red recombineering in E. coli","volume":"2829","author":"de Jong","year":"2024"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0265","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.3390\/v12121448","article-title":"Relocation of the attTn7 transgene insertion site in bacmid DNA enhances baculovirus genome stability and recombinant protein expression in insect cells","volume":"12","author":"Pijlman","year":"2020","journal-title":"Viruses"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0270","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1006\/viro.2001.0854","article-title":"Autographa californica baculoviruses with large genomic deletions are rapidly generated in infected insect cells","volume":"283","author":"Pijlman","year":"2001","journal-title":"Virology"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0275","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms15411","article-title":"Covalently linked dengue virus envelope glycoprotein dimers reduce exposure of the immunodominant fusion loop epitope","volume":"8","author":"Rouvinski","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0280","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1038\/s41598-017-01097-5","article-title":"Temperature-dependent folding allows stable dimerization of secretory and virus-associated E proteins of dengue and Zika viruses in mammalian cells","volume":"7","author":"Slon Campos","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0285","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0127999","article-title":"Seroepidemiology of coxsackievirus A6, coxsackievirus A16, and enterovirus 71 infections among children and adolescents in Singapore, 2008-2010","volume":"10","author":"Ang","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0290","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1586\/14787210.2015.1058156","article-title":"Coxsackievirus A6: a new emerging pathogen causing hand, foot and mouth disease outbreaks worldwide","volume":"13","author":"Bian","year":"2015","journal-title":"Expert Rev. Anti-Infect. Ther."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0295","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.1002\/bit.25625","article-title":"Enhanced enterovirus 71 virus-like particle yield from a new baculovirus design","volume":"112","author":"Lin","year":"2015","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0300","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jtice.2017.10.020","article-title":"Production and purification of virus-like particles of different enterovirus subtypes as vaccines","volume":"82","author":"Lin","year":"2018","journal-title":"J. Taiwan Inst. Chem. Eng."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0305","doi-asserted-by":"crossref","first-page":"118","DOI":"10.3390\/pathogens14020118","article-title":"Optimization of enterovirus-like particle production and purification using design of experiments","volume":"14","author":"Kuijpers","year":"2025","journal-title":"Pathogens"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0310","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1012873","article-title":"Enterovirus-like particles encapsidate RNA and exhibit decreased stability due to lack of maturation","volume":"21","author":"Kuijpers","year":"2025","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0315","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.jviromet.2012.11.011","article-title":"Efficient production of foot-and-mouth disease virus empty capsids in insect cells following down regulation of 3C protease activity","volume":"187","author":"Porta","year":"2013","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0320","doi-asserted-by":"crossref","first-page":"7746","DOI":"10.1128\/jvi.68.12.7746-7756.1994","article-title":"Identification and characterization of vlf-1, a baculovirus gene involved in very late gene expression","volume":"68","author":"McLachlin","year":"1994","journal-title":"J. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0325","doi-asserted-by":"crossref","first-page":"5040","DOI":"10.1128\/jvi.71.7.5040-5050.1997","article-title":"Identification and characterization of a baculovirus structural protein, VP1054, required for nucleocapsid formation","volume":"71","author":"Olszewski","year":"1997","journal-title":"J. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0330","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1128\/jvi.68.2.1075-1084.1994","article-title":"A mutant baculovirus with a temperature-sensitive IE-1 transregulatory protein","volume":"68","author":"Ribeiro","year":"1994","journal-title":"J. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0335","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1007\/s00253-005-0127-2","article-title":"Expression of spider flagelliform silk protein in Bombyx mori cell line by a novel Bac-to-Bac\/BmNPV baculovirus expression system","volume":"71","author":"Miao","year":"2006","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0340","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/j.jbiotec.2007.01.003","article-title":"Modeling rotavirus-like particles production in a baculovirus expression vector system: infection kinetics, baculovirus DNA replication, mRNA synthesis and protein production","volume":"128","author":"Rold\u00e3o","year":"2007","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0345","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0168-1656(00)00403-X","article-title":"Secretory pathway limits the enhanced expression of classical swine fever virus E2 glycoprotein in insect cells","volume":"86","author":"van Oers","year":"2001","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0350","doi-asserted-by":"crossref","first-page":"5496","DOI":"10.1016\/j.vaccine.2014.07.074","article-title":"Technology transfer and scale-up of the Flublok\u00ae recombinant hemagglutinin (HA) influenza vaccine manufacturing process","volume":"32","author":"Buckland","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0355","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jviromet.2005.08.021","article-title":"A rapid method for estimation of baculovirus titer based on viable cell size","volume":"132","author":"Janakiraman","year":"2006","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0360","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1023\/A:1005688417525","article-title":"Cell size as a tool to predict the production of recombinant protein by the insect-cell baculovirus expression system","volume":"23","author":"Palomares","year":"2001","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0365","doi-asserted-by":"crossref","first-page":"225","DOI":"10.3390\/vaccines11020225","article-title":"Evaluation of virus-free manufacture of recombinant proteins using CRISPR-mediated gene disruption in baculovirus-infected insect cells","volume":"11","author":"Bruder","year":"2023","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0370","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0289178","article-title":"Evaluation of an inducible knockout system in insect cells based on co-infection and CRISPR\/Cas9","volume":"18","author":"Hausjell","year":"2023","journal-title":"PLoS One"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0375","doi-asserted-by":"crossref","first-page":"785","DOI":"10.2144\/000113238","article-title":"A rapid method for titrating baculovirus stocks using the Sf-9 Easy Titer cell line","volume":"47","author":"Hopkins","year":"2009","journal-title":"Biotechniques"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0380","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1002\/pro.3943","article-title":"UCSF ChimeraX: structure visualization for researchers, educators, and developers","volume":"30","author":"Pettersen","year":"2021","journal-title":"Protein Sci."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0385","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1093\/bioinformatics\/btu744","article-title":"DISOPRED3: precise disordered region predictions with annotated protein-binding activity","volume":"31","author":"Jones","year":"2015","journal-title":"Bioinformatics"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0390","doi-asserted-by":"crossref","first-page":"4438","DOI":"10.1038\/s41467-021-24773-7","article-title":"flDPnn: accurate intrinsic disorder prediction with putative propensities of disorder functions","volume":"12","author":"Hu","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0395","doi-asserted-by":"crossref","first-page":"D458","DOI":"10.1093\/nar\/gkaa937","article-title":"SMART: recent updates, new developments and status in 2020","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0400","doi-asserted-by":"crossref","first-page":"D493","DOI":"10.1093\/nar\/gkx922","article-title":"20 years of the SMART protein domain annotation resource","volume":"46","author":"Letunic","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0405","doi-asserted-by":"crossref","first-page":"W276","DOI":"10.1093\/nar\/gkac240","article-title":"Search and sequence analysis tools services from EMBL-EBI in 2022","volume":"50","author":"Madeira","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0410","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1093\/bioinformatics\/btp033","article-title":"Jalview Version 2-a multiple sequence alignment editor and analysis workbench","volume":"25","author":"Waterhouse","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0415","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/978-1-0716-0191-4_5","article-title":"Using a parallel micro-cultivation system (micro-matrix) as a process development tool for cell culture applications","volume":"2095","author":"Wiegmann","year":"2020","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0420","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0425","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0108516","article-title":"Verifying the stability of selected genes for normalization in qPCR experiments of Spodoptera frugiperda cells during AcMNPV infection","volume":"9","author":"Salem","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0430","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2020.25.46.2001904","article-title":"First autochthonous human West Nile virus infections in the Netherlands, July to August 2020","volume":"25","author":"Vlaskamp","year":"2020","journal-title":"Eurosurveillance"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0435","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01415-20","article-title":"Zika virus-like particles bearing a covalent dimer of envelope protein protect mice from lethal challenge","volume":"95","author":"De Lorenzo","year":"2020","journal-title":"J. Virol."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0440","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.virol.2016.06.006","article-title":"Production of immunogenic West Nile virus-like particles using a herpes simplex virus 1 recombinant vector","volume":"496","author":"Taylor","year":"2016","journal-title":"Virology"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0445","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0014-5793(02)03659-1","article-title":"Enhancement of protein expression in insect cells by a lobster tropomyosin cDNA leader sequence","volume":"532","author":"Sano","year":"2002","journal-title":"FEBS Lett."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0450","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1186\/1471-2105-14-346","article-title":"Disulfide by Design 2.0: a web-based tool for disulfide engineering in proteins","volume":"14","author":"Craig","year":"2013","journal-title":"BMC Bioinformatics"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0455","doi-asserted-by":"crossref","first-page":"548","DOI":"10.4269\/ajtmh.1982.31.548","article-title":"Identification of distinct antigenic determinants on dengue-2 virus using monoclonal antibodies","volume":"31","author":"Gentry","year":"1982","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0460","first-page":"297","article-title":"Production of Chikungunya virus-like particles and subunit vaccines in insect cell","volume":"1426","author":"Metz","year":"2016"},{"key":"10.1016\/j.tibtech.2025.04.004_bb0465","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1002\/bit.20178","article-title":"Evaluation of baculovirus expression vectors with enhanced stability in continuous cascaded insect-cell bioreactors","volume":"87","author":"Pijlman","year":"2004","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.tibtech.2025.04.004_bb0470","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1186\/1743-422X-8-353","article-title":"Functional processing and secretion of Chikungunya virus E1 and E2 glycoproteins in insect cells","volume":"8","author":"Metz","year":"2011","journal-title":"Virol. J."}],"container-title":["Trends in Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167779925001313?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167779925001313?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:39:00Z","timestamp":1762256340000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167779925001313"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":94,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["S0167779925001313"],"URL":"https:\/\/doi.org\/10.1016\/j.tibtech.2025.04.004","relation":{},"ISSN":["0167-7799"],"issn-type":[{"value":"0167-7799","type":"print"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Engineered thermoswitch in the baculovirus expression vector system for production of virus-like particle vaccines with minimized baculovirus contaminants","name":"articletitle","label":"Article Title"},{"value":"Trends in Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tibtech.2025.04.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}