{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T09:42:33Z","timestamp":1770716553088,"version":"3.49.0"},"reference-count":40,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2001,11,1]],"date-time":"2001-11-01T00:00:00Z","timestamp":1004572800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,8,22]],"date-time":"2013-08-22T00:00:00Z","timestamp":1377129600000},"content-version":"vor","delay-in-days":4312,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochimica et Biophysica Acta (BBA) - Biomembranes"],"published-print":{"date-parts":[[2001,11]]},"DOI":"10.1016\/s0005-2736(01)00389-3","type":"journal-article","created":{"date-parts":[[2002,10,14]],"date-time":"2002-10-14T13:45:43Z","timestamp":1034603143000},"page":"23-37","source":"Crossref","is-referenced-by-count":123,"title":["On the mechanisms of internalization and intracellular delivery mediated by pH-sensitive liposomes"],"prefix":"10.1016","volume":"1515","author":[{"given":"S\u00e9rgio","family":"Sim\u00f5es","sequence":"first","affiliation":[]},{"given":"Vladimir","family":"Slepushkin","sequence":"additional","affiliation":[]},{"given":"Nejat","family":"D\u00fczg\u00fcnes","sequence":"additional","affiliation":[]},{"given":"Maria C.","family":"Pedroso de Lima","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"key":"10.1016\/S0005-2736(01)00389-3_BIB1","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/0014-5793(85)80210-6","article-title":"pH-sensitive liposomes mediate cytoplasmic delivery of encapsulated macromolecules","volume":"179","author":"Straubinger","year":"1985","journal-title":"FEBS Lett."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB2","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1083\/jcb.101.2.582","article-title":"Efficient cytoplasmic delivery of a fluorescent dye by pH-sensitive immunoliposomes","volume":"101","author":"Connor","year":"1985","journal-title":"J. Cell Biol."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB3","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1023\/A:1015908831507","article-title":"Efficiency of cytoplasmic delivery by pH-sensitive liposomes to cells in culture","volume":"7","author":"Chu","year":"1990","journal-title":"Pharm. Res."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB4","doi-asserted-by":"crossref","unstructured":"N. D\u00fczg\u00fcnes, R.M. Straubinger, P.A. Baldwin, D. Papahadjopoulos, pH-sensitive liposomes: introduction of foreign substances into cells, in: J. Wilschut, D. Hoekstra (Eds.), Membrane Fusion, Marcel Dekker, New York, 1991, pp. 713\u2013730.","DOI":"10.1201\/9780367811525-31"},{"key":"10.1016\/S0005-2736(01)00389-3_BIB5","unstructured":"D. Collins, pH-sensitive liposomes as tools for cytoplasmic delivery, in: J.R. Philippot, F. Schuber (Eds.), Liposomes as Tools in Basic Research and Industry, Vol. 13, CRS Press, 1995, pp. 201\u2013214."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB6","doi-asserted-by":"crossref","first-page":"361","DOI":"10.3109\/08982109409037050","article-title":"pH-sensitive liposomes","volume":"4","author":"Chu","year":"1994","journal-title":"J. Liposome Res."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB7","doi-asserted-by":"crossref","first-page":"1646","DOI":"10.1021\/bi00328a012","article-title":"Effect of replacement of the hydroxyl group of cholesterol and tocopherol on the thermotropic behavior of phospholipid membranes","volume":"24","author":"Lai","year":"1985","journal-title":"Biochemistry"},{"key":"10.1016\/S0005-2736(01)00389-3_BIB8","doi-asserted-by":"crossref","first-page":"201","DOI":"10.3109\/08982109309148213","article-title":"pH-sensitive liposomes","volume":"3","author":"Torchilin","year":"1993","journal-title":"J. Liposome Res."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB9","first-page":"374","article-title":"Delivery of superoxide dismutase to pulmonary epithelium via pH-sensitive liposomes","volume":"268","author":"Briscoe","year":"1995","journal-title":"Am. J. Physiol."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB10","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0168-3659(97)00042-4","article-title":"Delivery of antisense oligonucleotides by means of pH-sensitive liposomes","volume":"48","author":"De Oliveira","year":"1997","journal-title":"J. Control. Release"},{"key":"10.1016\/S0005-2736(01)00389-3_BIB11","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/S0005-2736(98)00067-4","article-title":"pH-sensitive liposomes as carrier for oligonucleotides: a physico-chemical study of the interaction between DOPE and a 15-mer oligonucleotide in quasi-anhydrous samples","volume":"1372","author":"De Oliveira","year":"1998","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/S0005-2736(01)00389-3_BIB12","doi-asserted-by":"crossref","first-page":"2432","DOI":"10.1046\/j.1432-1327.2000.01255.x","article-title":"Inhibition of inositol uptake in astrocytes by antisense oligonucleotides delivered by pH-sensitive liposomes","volume":"267","author":"Lubrich","year":"2000","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB13","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0168-3659(99)00135-2","article-title":"Enhanced folate receptor mediated gene therapy using a novel pH-sensitive lipid formulation","volume":"64","author":"Reddy","year":"2000","journal-title":"J. Control. Release"},{"key":"10.1016\/S0005-2736(01)00389-3_BIB14","doi-asserted-by":"crossref","unstructured":"N. D\u00fczg\u00fcnes, S. Sim\u00f5es, V. Slepushkin, E. Pretzer, J.J. Rossi, E. DeClercq, V.P. Antao, M.L. Collins, M.C. Pedroso de Lima, Enhanced inhibition of HIV-1 replication in macrophages by antisense oligonucleotides, ribozymes and acyclic nucleoside analogs delivered in pH-sensitive liposomes, Nucleotides, Nucleosides Nucleic Acids (2001) in press.","DOI":"10.1081\/NCN-100002327"},{"key":"10.1016\/S0005-2736(01)00389-3_BIB15","doi-asserted-by":"crossref","first-page":"2382","DOI":"10.1074\/jbc.272.4.2382","article-title":"Sterically stabilized pH-sensitive liposomes: intracellular delivery of aqueous contents and prolonged circulation in vivo","volume":"272","author":"Slepushkin","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB16","doi-asserted-by":"crossref","first-page":"4194","DOI":"10.1073\/pnas.75.9.4194","article-title":"Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation","volume":"75","author":"Szoka","year":"1978","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/S0005-2736(01)00389-3_BIB17","unstructured":"R.R.C. New, Characterization of liposomes, in: R.R.C. New (Ed.), Liposomes: a Practical Approach, Oxford University Press, Oxford, 1990, pp. 105\u2013161."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB18","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/S0021-9258(18)70226-3","article-title":"Phosphorus assay in column chromatography","volume":"254","author":"Bartlett","year":"1959","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB19","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/0378-5173(93)90213-Y","article-title":"Liposomal L-asparaginase: in vitro evaluation","volume":"96","author":"Cruz","year":"1993","journal-title":"Int. J. Pharm."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB20","unstructured":"G.S. Harfield, R.C. Bunker, Measuring zeta potential and electrophoretic mobility by laser doppler velocimetry, Filtration Separation Nov.\/Dec. (1988) 412\u2013415."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB21","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1016\/S0006-3495(92)81897-0","article-title":"Sterically stabilized liposomes reduction in electrophoretic mobility but not electrostatic surface potential","volume":"61","author":"Woodle","year":"1992","journal-title":"Biophys. J."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0378-5173(96)04486-9","article-title":"Steric stabilization of nanoparticles: size and surface properties","volume":"138","author":"Louren\u00e7o","year":"1996","journal-title":"Int. J. Pharm."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB23","doi-asserted-by":"crossref","first-page":"3327","DOI":"10.1073\/pnas.75.7.3327","article-title":"Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation by various agents","volume":"75","author":"Ohkuma","year":"1978","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/S0005-2736(01)00389-3_BIB24","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1006\/viro.1993.1197","article-title":"Long-term noncytopathic productive infection of the human monocytic leukemia cell line THP-1 by human immunodeficiency virus type 1 (HIV-1)","volume":"193","author":"Konopka","year":"1993","journal-title":"Virology"},{"key":"10.1016\/S0005-2736(01)00389-3_BIB25","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1021\/bi00054a021","article-title":"Quantitative analysis of liposome-cell interactions in vitro: rate constants of binding and endocytosis with suspension and adherent J774 cells and human monocytes","volume":"32","author":"Lee","year":"1993","journal-title":"Biochemistry"},{"key":"10.1016\/S0005-2736(01)00389-3_BIB26","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1038\/nbt0696-760","article-title":"pH-sensitive cationic liposomes: a new synthetic virus-like vector","volume":"14","author":"Budker","year":"1996","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB27","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/(SICI)1097-4644(199607)62:1<27::AID-JCB4>3.0.CO;2-3","article-title":"In vitro fusion of endocytic vesicles: effects of reagents that alter endosomal pH","volume":"62","author":"Pless","year":"1996","journal-title":"J. Cell. Biochem."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB28","doi-asserted-by":"crossref","first-page":"21940","DOI":"10.1016\/S0021-9258(18)45829-2","article-title":"The cytotoxic action of diphtheria toxin and its degradation in intact vero cells are inhibited by bafilomycin A1, a specific inhibitor of vacuolar-type H+-ATPase","volume":"265","author":"Umata","year":"1990","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB29","doi-asserted-by":"crossref","first-page":"17707","DOI":"10.1016\/S0021-9258(19)47429-2","article-title":"Bafilomycin A1, a specific inhibitor of vacuolar-type H+-ATPase, inhibits acidification and protein degradation in lysosomes of cultured cells","volume":"266","author":"Yoshimori","year":"1991","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB30","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1128\/AAC.41.1.212","article-title":"Bafilomycin A1 and intracellular multiplication of Legionella pneumophila","volume":"41","author":"Cattani","year":"1997","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB31","unstructured":"H. Talsma, Preparation, Characterization and Stabilization of Liposomes, Ph.D. Thesis, Faculty of Pharmacy, Utrecht, 1991."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB32","unstructured":"D. Lasic, Liposomes: from Physics to Therapeutics, Elsevier Science, Amsterdam, 1993."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB33","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1093\/oxfordjournals.jbchem.a124717","article-title":"Kinetic analysis of endocytosis and intracellular fate of liposomes in single macrophages","volume":"117","author":"Yoshimura","year":"1995","journal-title":"J. Biochem."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB34","doi-asserted-by":"crossref","first-page":"17823","DOI":"10.1074\/jbc.270.30.17823","article-title":"In vitro fusion of reticulocyte endocytic vesicles with liposomes","volume":"270","author":"Vidal","year":"1995","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB35","doi-asserted-by":"crossref","unstructured":"N. D\u00fczg\u00fcnes, J. Wilschut, D. Papahadjopoulos, Control of membrane fusion by divalent cations, phospholipid head-groups and proteins, in: F. Conti, W.E. Blomberg, J. de Gier, F. Pocchiari (Eds.), Physical Methods on Biological Membranes and Their Model Systems, Plenum, New York, 1988, pp. 193\u2013218.","DOI":"10.1007\/978-1-4684-7538-8_14"},{"key":"10.1016\/S0005-2736(01)00389-3_BIB36","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/0168-3659(92)90124-A","article-title":"Destabilization of pH-sensitive liposomes in the presence of human erythrocyte ghosts","volume":"20","author":"Greidziak","year":"1992","journal-title":"J. Control. Release"},{"key":"10.1016\/S0005-2736(01)00389-3_BIB37","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/0005-2736(90)90365-U","article-title":"Endocytosis of liposomes by macrophages: binding, acidification and leakage of liposomes monitored by a new fluorescence assay","volume":"1024","author":"Daleke","year":"1990","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/S0005-2736(01)00389-3_BIB38","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/BF01901634","article-title":"Role of hydrophobic interactions in the fusion activity of influenza and Sendai viruses towards model membranes","volume":"14","author":"Ramalho-Santos","year":"1994","journal-title":"Biosci. Rep."},{"key":"10.1016\/S0005-2736(01)00389-3_BIB39","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/BF02664772","article-title":"Biophysical studies and intracellular destabilization of pH-sensitive liposomes","volume":"37","author":"Bambeke","year":"2000","journal-title":"Lipids"},{"key":"10.1016\/S0005-2736(01)00389-3_BIB40","doi-asserted-by":"crossref","first-page":"3099","DOI":"10.1021\/bi00334a005","article-title":"H+- and Ca2+-induced fusion and destabilization of liposomes","volume":"24","author":"Ellens","year":"1985","journal-title":"Biochemistry"}],"container-title":["Biochimica et Biophysica Acta (BBA) - Biomembranes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0005273601003893?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0005273601003893?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,5,7]],"date-time":"2021-05-07T06:21:12Z","timestamp":1620368472000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0005273601003893"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,11]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,11]]}},"alternative-id":["S0005273601003893"],"URL":"https:\/\/doi.org\/10.1016\/s0005-2736(01)00389-3","relation":{},"ISSN":["0005-2736"],"issn-type":[{"value":"0005-2736","type":"print"}],"subject":[],"published":{"date-parts":[[2001,11]]}}}