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The 49 amino acid presequence transit peptide (psALAS) for murine erythroid ALAS was chemically synthesized, and circular dichroism and <jats:sup>1<\/jats:sup>H nuclear magnetic resonance (NMR) spectroscopies used to determine structural elements in trifluoroethanol\/H<jats:sub>2<\/jats:sub>O solutions and micellar environments. A well defined amphipathic \u03b1\u2010helix, spanning L22 to F33, was present in psALAS in 50% trifluoroethanol. Further, a short \u03b1\u2010helix, defined by A5\u2013L8, was also apparent in the 26 amino acid N\u2010terminus peptide, when its structure was determined in sodium dodecyl sulfate. Heme inhibition of ALAS mitochondrial import has been reported to be mediated through cysteine residues in presequence heme regulatory motifs (HRMs). A UV\/visible and <jats:sup>1<\/jats:sup>H NMR study of hemin and psALAS indicated that a heme\u2013peptide interaction occurs and demonstrates, for the first time, that heme interacts with the HRMs of psALAS.<\/jats:p>","DOI":"10.1016\/s0014-5793(01)02818-6","type":"journal-article","created":{"date-parts":[[2002,10,14]],"date-time":"2002-10-14T15:56:01Z","timestamp":1034610961000},"page":"325-331","source":"Crossref","is-referenced-by-count":25,"title":["The solution structure and heme binding of the presequence of murine 5\u2010aminolevulinate synthase"],"prefix":"10.1002","volume":"505","author":[{"given":"Brian J","family":"Goodfellow","sequence":"first","affiliation":[]},{"given":"Jorge S","family":"Dias","sequence":"additional","affiliation":[]},{"given":"Gl\u00f3ria C","family":"Ferreira","sequence":"additional","affiliation":[]},{"given":"Peter","family":"Henklein","sequence":"additional","affiliation":[]},{"given":"Victor","family":"Wray","sequence":"additional","affiliation":[]},{"given":"Anjos L","family":"Macedo","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,8,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02110030"},{"key":"e_1_2_7_3_1","unstructured":"Kappas A. 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