{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T09:59:09Z","timestamp":1760954349672},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1998,12,22]],"date-time":"1998-12-22T00:00:00Z","timestamp":914284800000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1998,12,4]]},"abstract":"<jats:p>\n<jats:sup>13<\/jats:sup>C has become an important tracer isotope for studies of intermediary metabolism. Information about relative flux through pathways is encoded by the distribution of <jats:sup>13<\/jats:sup>C isotopomers in an intermediate pool such as glutamate. This information is commonly decoded either by mass spectrometry or by measuring relative multiplet areas in a <jats:sup>13<\/jats:sup>C NMR spectrum. We demonstrate here that groups of glutamate <jats:sup>13<\/jats:sup>C isotopomers may be quantified by indirect detection of protons in a 2D HMQC\u2010TOCSY NMR spectrum and that fitting of these data to a metabolic model provides an identical measure of the <jats:sup>13<\/jats:sup>C fractional enrichment of acetyl\u2010CoA and relative anaplerotic flux to that given by direct <jats:sup>13<\/jats:sup>C NMR analysis. The sensitivity gain provided by HMQC\u2010TOCSY spectroscopy will allow an extension of <jats:sup>13<\/jats:sup>C isotopomer analysis to tissue samples not amenable to direct <jats:sup>13<\/jats:sup>C detection (\u223c10 mg soleus muscle) and to tissue metabolites other than glutamate that are typically present at lower concentrations.<\/jats:p>","DOI":"10.1016\/s0014-5793(98)01491-4","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T17:52:28Z","timestamp":1027619548000},"page":"382-386","source":"Crossref","is-referenced-by-count":24,"title":["<sup>13<\/sup>C isotopomer analysis of glutamate by heteronuclear multiple quantum coherence\u2010total correlation spectroscopy (HMQC\u2010TOCSY)"],"prefix":"10.1002","volume":"440","author":[{"given":"Rui A.","family":"Carvalho","sequence":"first","affiliation":[]},{"given":"F.Mark H.","family":"Jeffrey","sequence":"additional","affiliation":[]},{"given":"A.Dean","family":"Sherry","sequence":"additional","affiliation":[]},{"given":"Craig R.","family":"Malloy","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1998,12,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(87)81556-9"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)46969-4"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1990.259.6.C987"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)00764-3"},{"key":"e_1_2_6_6_1","unstructured":"Jones J.G. 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