{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T16:34:47Z","timestamp":1768322087238,"version":"3.49.0"},"reference-count":35,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2000,6,1]],"date-time":"2000-06-01T00:00:00Z","timestamp":959817600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomaterials"],"published-print":{"date-parts":[[2000,6]]},"DOI":"10.1016\/s0142-9612(99)00284-7","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T02:00:28Z","timestamp":1027648828000},"page":"1087-1094","source":"Crossref","is-referenced-by-count":111,"title":["Human bone cell cultures in biocompatibility testing. Part I: osteoblastic differentiation of serially passaged human bone marrow cells cultured in \u03b1-MEM and in DMEM"],"prefix":"10.1016","volume":"21","author":[{"given":"M.J","family":"Coelho","sequence":"first","affiliation":[]},{"given":"A","family":"Trigo Cabral","sequence":"additional","affiliation":[]},{"given":"M.H","family":"Fernandes","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"key":"10.1016\/S0142-9612(99)00284-7_BIB1","first-page":"131","article-title":"The cell and molecular biological approach to biomaterial research","volume":"8","author":"Kirkpatrick","year":"1997","journal-title":"J Mater Sci"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB2","first-page":"9","article-title":"Theoretical and practical aspects of testing potential biomaterials in vitro","volume":"1","author":"Kirkpatrick","year":"1990","journal-title":"J Mater"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB3","first-page":"13","article-title":"A critical view of current and proposed methodologies for biocompatibility testing","volume":"4","author":"Kirkpatrick","year":"1992","journal-title":"Regulatory Affairs"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB4","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0267-6605(94)90081-7","article-title":"Cell culture methods for testing biocompatibility","volume":"15","author":"Pizzoferrato","year":"1994","journal-title":"Clin Mater"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB5","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1002\/bit.260430707","article-title":"Mini-review: osteoblasts","volume":"43","author":"Bizios","year":"1994","journal-title":"Biotechnol Bioeng"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB6","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/0014-4827(65)90211-9","article-title":"The limited in vitro lifetime of human diploid cell strains","volume":"37","author":"Hayflick","year":"1965","journal-title":"Exp Cell Res"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB7","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1007\/BF02554868","article-title":"Characterization of human bone cells in culture","volume":"37","author":"Aufmkolk","year":"1987","journal-title":"Calcif Tissue Int"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB8","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/S0169-6009(08)80002-5","article-title":"Long-term cultivation of human osteoblasts","volume":"20","author":"Schmidt","year":"1993","journal-title":"Bone Mineral"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB9","unstructured":"Freshney RI, editor. The culture environment: substrate, gas phase, medium and temperature. In: Culture of animal cells: a manual of basic technique. New York: Wiley-Liss, 1994. p. 71\u2013101."},{"key":"10.1016\/S0142-9612(99)00284-7_BIB10","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1002\/jbmr.5650050406","article-title":"Influence of experimental conditions on osteoblast activity in human primary bone cell cultures","volume":"5","author":"Chavassieux","year":"1990","journal-title":"J Bone Miner Res"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB11","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1006\/abbi.1993.1311","article-title":"Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)","volume":"303","author":"Berridge","year":"1993","journal-title":"Arch Biochem Biophys"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB12","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1177\/6.1.22","article-title":"Histochemical methods for calcium","volume":"6","author":"McGee-Russel","year":"1958","journal-title":"J Histochem Cytochem"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB13","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1007\/BF00225804","article-title":"Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats","volume":"254","author":"Maniatopoulos","year":"1988","journal-title":"Cell Tissue Res"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB14","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.1210\/endo-121-6-1985","article-title":"Physiological concentrations of glucocorticoids stimulate formation of bone nodules from isolated rat calvaria cells in vitro","volume":"121","author":"Bellows","year":"1987","journal-title":"Endocrinology"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB15","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1002\/jcp.1041430203","article-title":"Factors that promote progressive development of the osteoblast phenotype in cultured fetal rat calvaria cells","volume":"143","author":"Aronow","year":"1990","journal-title":"J Cell Physiol"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB16","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/0169-6009(90)90109-S","article-title":"In vitro mineralization of osteoblastic cells derived from human bone","volume":"8","author":"Gotoh","year":"1990","journal-title":"Bone Mineral"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB17","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1210\/en.134.1.277","article-title":"Differentiation of human bone marrow osteogenic stromal cells in vitro. Induction of the osteoblast phenotype by dexamethasone","volume":"134","author":"Cheng","year":"1994","journal-title":"Endocrinology"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB18","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/8756-3282(95)00112-Q","article-title":"Human bone tissue formation in diffusion chamber culture in vivo by bone derived cells and marrow stromal fibroblastic cells","volume":"16","author":"Gundle","year":"1995","journal-title":"Bone"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB19","first-page":"61","article-title":"Mineralization in serially passaged human alveolar bone cells","volume":"8","author":"Fernandes","year":"1997","journal-title":"J Mater Sci"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB20","first-page":"233","article-title":"The use of rat, rabbit or human bone marrow derived cells for cytocompatibility evaluation of metallic elements","volume":"8","author":"Tom\u00e1s","year":"1997","journal-title":"J Mater Sci"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB21","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1002\/(SICI)1097-4636(199902)44:2<176::AID-JBM8>3.0.CO;2-6","article-title":"In vitro osteoblastic differentiation of human bone marrow cells in the presence of metal ions","volume":"44","author":"Morais","year":"1999","journal-title":"J Biomed Mater Res"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB22","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1002\/jcp.1041430304","article-title":"Progressive development of the rat osteoblast phenotype in vitro","volume":"143","author":"Owen","year":"1990","journal-title":"J Cell Physiol"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB23","series-title":"Handbook of experimental pharmacology","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/978-3-642-77991-6_4","article-title":"Osteoblasts","author":"Martin","year":"1993"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB24","series-title":"Cellular and molecular biology of bone","first-page":"47","article-title":"Molecular mechanisms mediating developmental and hormone-regulated expression of genes in osteoblasts","author":"Stein","year":"1993"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB25","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1002\/jcp.1041580322","article-title":"Cellular expression of bone-related proteins during in vitro osteogenesis in rat bone marrow stromal cell cultures","volume":"158","author":"Malaval","year":"1994","journal-title":"J Cell Phys"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB26","first-page":"290","article-title":"Molecular mechanisms mediated proliferation\u2013differentiation interrelationships during progressive development of the osteoblast phenotype","volume":"4","author":"Stein","year":"1995","journal-title":"End Rev"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB27","series-title":"Cellular and molecular biology of bone","first-page":"1","article-title":"Osteoblastic cell lineage","author":"Aubin","year":"1993"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB28","series-title":"Handbook of experimental pharmacology","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/978-3-642-77991-6_8","article-title":"Mineralization","author":"Anderson","year":"1993"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB29","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0169-6009(91)90100-E","article-title":"Initiation and progression of mineralization of bone nodules formed in vitro","volume":"14","author":"Bellows","year":"1991","journal-title":"Bone Mineral"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB30","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1002\/(SICI)1097-4636(199811)42:2<199::AID-JBM5>3.0.CO;2-K","article-title":"van Blitterswijk. Decreased consumption of calcium and phosphorus during in vitro biomineralization and biologically-induced deposition of Ni and Cr in the presence of stainless steel corrosion products","volume":"42","author":"Morais","year":"1998","journal-title":"J Biomed Mater Res"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB31","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/S0142-9612(97)00234-2","article-title":"Effects of AISI 316L corrosion products in in vitro bone formation","volume":"19","author":"Morais","year":"1998","journal-title":"Biomaterials"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB32","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/0169-6009(92)90707-K","article-title":"Inorganic phosphate added exogenously or released from \u03b2-glycerophosphate initiates mineralization of osteoid nodules in vitro","volume":"17","author":"Bellows","year":"1992","journal-title":"Bone Mineral"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB33","unstructured":"Freshney RI, editor. Biology of the cultured cell. In: Culture of animal cells: a manual of basic technique. New York: Wiley-Liss, 1994. p. 9\u201316."},{"key":"10.1016\/S0142-9612(99)00284-7_BIB34","first-page":"60","article-title":"The role of ascorbic acid in mesenchymal differentiation","volume":"50","author":"Franceschi","year":"1992","journal-title":"Nutr Rev"},{"key":"10.1016\/S0142-9612(99)00284-7_BIB35","doi-asserted-by":"crossref","first-page":"C1430","DOI":"10.1152\/ajpcell.1995.268.6.C1430","article-title":"Requirement for Na+-dependent ascorbic acid transport in osteoblastic function","volume":"268","author":"Franceschi","year":"1995","journal-title":"Am J Physiol"}],"container-title":["Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0142961299002847?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0142961299002847?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,2,5]],"date-time":"2020-02-05T00:56:31Z","timestamp":1580864191000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0142961299002847"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,6]]},"references-count":35,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2000,6]]}},"alternative-id":["S0142961299002847"],"URL":"https:\/\/doi.org\/10.1016\/s0142-9612(99)00284-7","relation":{},"ISSN":["0142-9612"],"issn-type":[{"value":"0142-9612","type":"print"}],"subject":[],"published":{"date-parts":[[2000,6]]}}}