{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T16:24:39Z","timestamp":1761323079796},"reference-count":58,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[1997,6,1]],"date-time":"1997-06-01T00:00:00Z","timestamp":865123200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Current Opinion in Biotechnology"],"published-print":{"date-parts":[[1997,6]]},"DOI":"10.1016\/s0958-1669(97)80012-2","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T10:32:55Z","timestamp":1027593175000},"page":"328-334","source":"Crossref","is-referenced-by-count":34,"title":["Understanding and advancing wastewater treatment"],"prefix":"10.1016","volume":"8","author":[{"given":"Alfons JM","family":"Stams","sequence":"first","affiliation":[]},{"given":"Stefanie JWH","family":"Oude Elferink","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"key":"10.1016\/S0958-1669(97)80012-2_BIB1_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/BF01570068","article-title":"Environmental applications of immobilized microbial cells: a review","volume":"16","author":"Cassidy","year":"1996","journal-title":"J Indust Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB2","article-title":"Biofilm structure and kinetics","author":"Bishop","year":"1997","journal-title":"Wat Res"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB3","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1146\/annurev.mi.49.100195.003431","article-title":"Microbial biofilms","volume":"49","author":"Costerton","year":"1995","journal-title":"Annu Rev Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/BF02011566","article-title":"Behavioral strategies of surface-colonizing bacteria","volume":"14","author":"Lawrence","year":"1995","journal-title":"Adv Microbiol Ecol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB5_1","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1002\/(SICI)1097-0290(19960120)49:2<172::AID-BIT6>3.0.CO;2-N","article-title":"Mathematical modeling of mixed-culture biofilms","volume":"49","author":"Wanner","year":"1996","journal-title":"Biotechnol Bioeng"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB6_1","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/0165-9936(95)97056-7","article-title":"Microscale biosensors for environmental monitoring","volume":"14","author":"Damgaard","year":"1995","journal-title":"Trends Anal Chem"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB7","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/0273-1223(95)00174-L","article-title":"The use of microsensors to determine population distributions in UASB aggregates","volume":"31","author":"Lens","year":"1995","journal-title":"Wat Sci Tech"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB8_1","doi-asserted-by":"crossref","first-page":"4641","DOI":"10.1128\/AEM.62.12.4641-4647.1996","article-title":"Structure and function of a nitrifying biofilm as determined by in situ hybridization and the use of microelectrodes","volume":"62","author":"Schramm","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB9","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.2175\/106143096X128504","article-title":"Evaluation of substrate and pH effects in a nitrifying biofilm","volume":"68","author":"Zhang","year":"1996","journal-title":"Wat Environ Res"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB10","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/0167-7012(95)00085-2","article-title":"Comparison of microscope techniques for the examination of biofilms","volume":"25","author":"Surman","year":"1996","journal-title":"J Microbiol Methods"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB11","first-page":"505","article-title":"Manipulation and mapping of microbes with antibodies","author":"Macario","year":"1993"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB12","doi-asserted-by":"crossref","first-page":"3050","DOI":"10.1128\/AEM.62.8.3050-3052.1996","article-title":"Quantification of Dehalospirillum multivorans in mixed-culture biofilms with an enzyme-linked immunosorbent assay","volume":"62","author":"Bauer-Kreisel","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB13_1","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1128\/MR.60.4.641-696.1996","article-title":"Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses","volume":"60","author":"Davey","year":"1996","journal-title":"Microbiol Rev"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB14","first-page":"147","article-title":"Analysis of microbial communities within sediments using biomarkers","author":"Parkes","year":"1987"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB15_1","first-page":"247","article-title":"Approaches for identification of microorganisms","volume":"62","author":"B\u00f6ttger","year":"1996","journal-title":"ASM News"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB16","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1574-6941.1994.tb00085.x","article-title":"Detection of the anaerobic dechlorinating microorganism Desulfomonile tiedjei in environmental matrices by its signature lipopolysaccharide branched long-chain hydroxy fatty acids","volume":"14","author":"Ringelberg","year":"1994","journal-title":"FEMS Microbiol Ecol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB17","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/0922-338X(95)94007-E","article-title":"Inference of methanogenic bacteria in wastewater digestor sludges by lipid component analysis","volume":"79","author":"Nishihara","year":"1995","journal-title":"J Ferment Bioeng"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB18","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/S0723-2020(11)80115-3","article-title":"Cellular fatty acid composition of Desulfovibrio species and its use in classification of sulfate reducing bacteria","volume":"15","author":"Vainshtein","year":"1992","journal-title":"Syst Appl Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB19","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.1574-6968.1994.tb06905.x","article-title":"Comparison of phylogenetic relationships based on phospholipid fatty acid profiles and ribosomal RNA sequence similarities among dissimilatory sulfate reducing bacteria","volume":"119","author":"Kohring","year":"1994","journal-title":"FEMS Microbiol Lett"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB20_1","year":"1996"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-4757-0611-6_1","article-title":"The analysis of natural microbial populations by ribosomal RNA sequences","volume":"9","author":"Pace","year":"1986","journal-title":"Adv Microbiol Ecol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB22","first-page":"463","article-title":"New perspective on the natural microbial world: molecular microbial ecology","volume":"62","author":"Pace","year":"1996","journal-title":"ASM News"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB23_1","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1128\/MR.59.1.143-169.1995","article-title":"Phylogenetic identification and in situ detection of individual microbial cells without cultivation","volume":"59","author":"Amann","year":"1995","journal-title":"Microbiol Rev"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB24_1","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1111\/j.1365-294X.1995.tb00254.x","article-title":"Application of phylogenetically based hybridization probes to microbial ecology","volume":"4","author":"Stahl","year":"1995","journal-title":"Mol Ecol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB25","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/0273-1223(95)00173-K","article-title":"Use of ribosomal RNA-based molecular probes for characterization of complex microbial communities in anaerobic biofilms","volume":"31","author":"Raskin","year":"1995","journal-title":"Wat Sci Tech"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB26","first-page":"205","article-title":"Development and application of nucleic acid probes","author":"Stahl","year":"1991"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB27_1","doi-asserted-by":"crossref","first-page":"2163","DOI":"10.1128\/AEM.62.6.2163-2168.1996","article-title":"Population dynamics of propionate-oxidizing bacteria under methanogenic and sulfidogenic conditions in anaerobic granular sludge","volume":"62","author":"Harmsen","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB28_1","doi-asserted-by":"crossref","first-page":"1656","DOI":"10.1128\/AEM.62.5.1656-1663.1996","article-title":"Detection and localization of syntrophic propionate-oxidizing bacteria in granular sludge by in situ hybridization using 16S rRNA-based oligonucleotide probes","volume":"62","author":"Harmsen","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB29","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1128\/AEM.60.4.1241-1248.1994","article-title":"Quantification of methanogenic groups in anaerobic biological reactors by oligonucleotide probe hybridization","volume":"60","author":"Raskin","year":"1994","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB30_1","doi-asserted-by":"crossref","first-page":"3847","DOI":"10.1128\/AEM.62.10.3847-3857.1996","article-title":"Competition and coexistence of sulfate-reducing and methanogenic populations in anaerobic biofilms","volume":"62","author":"Raskin","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB31","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1128\/AEM.60.3.792-800.1994","article-title":"Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge","volume":"60","author":"Wagner","year":"1994","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB32_1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0723-2020(11)80396-6","article-title":"In situ identification of ammonia-oxidizing bacteria","volume":"18","author":"Wagner","year":"1995","journal-title":"Syst Appl Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB33_1","doi-asserted-by":"crossref","first-page":"4329","DOI":"10.1128\/AEM.62.12.4329-4339.1996","article-title":"Population analysis in a denitrifying sand filter: conventional and in situ identification of Paracoccus spp. in methanol-fed biofilm","volume":"62","author":"Neefs","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB34_1","doi-asserted-by":"crossref","first-page":"3496","DOI":"10.1128\/jb.178.12.3496-3500.1996","article-title":"In situ visualization of high genetic diversity in a natural microbial community","volume":"178","author":"Amann","year":"1996","journal-title":"J Bacteriol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB35","doi-asserted-by":"crossref","first-page":"4074","DOI":"10.1128\/AEM.61.11.4074-4082.1995","article-title":"In situ PCR for visualization of microscale distribution of specific genes and gene products in prokaryotic communities","volume":"61","author":"Hodson","year":"1995","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB36","first-page":"177","article-title":"The polymerase chain reaction","author":"Giovannoni","year":"1991"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB37","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/0960-8524(94)90031-0","article-title":"Identification of anaerobic digester bacteria using a polymerase chain reaction method","volume":"47","author":"Ng","year":"1994","journal-title":"Bioresource Technol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB38","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/0922-338X(95)94742-A","article-title":"Polymerase chain reaction amplification and restriction length polymorphism analysis of 16S rRNA genes from methanogens","volume":"79","author":"Hiraishi","year":"1995","journal-title":"J Ferment Bioeng"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB39","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1128\/AEM.61.4.1444-1450.1995","article-title":"Detection of ammonium-oxidizing bacteria of the beta-subclass of the class Proteobacteria in aquatic samples with the PCR","volume":"61","author":"Voytek","year":"1995","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0273-1223(96)00001-7","article-title":"Molecular methods to study the organization of microbial communities","volume":"31","author":"Muyzer","year":"1995","journal-title":"Wat Sci Tech"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB41","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1128\/AEM.59.3.695-700.1993","article-title":"Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA","volume":"59","author":"Muyzer","year":"1993","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB42","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1023\/A:1000150219937","article-title":"Novel principles in the microbial conversion of nitrogen compounds","volume":"71","author":"Jetten","year":"1997","journal-title":"Antonie van Leeuwenhoek"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB43_1","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1128\/AEM.61.4.1246-1251.1995","article-title":"Anaerobic oxidation of ammonium is a biologically mediated process","volume":"61","author":"Van de Graaf","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB44","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1111\/j.1574-6976.1994.tb00131.x","article-title":"Biology of polyphosphate-accumulating bacteria involved in enhanced biological phosphorus removal","volume":"15","author":"Kortstee","year":"1994","journal-title":"FEMS Microbiol Rev"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB45","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1023\/A:1000150523030","article-title":"Metabolism of micro-organisms responsible for enhanced biological phosphorus removal from wastewater","volume":"71","author":"Van Loosdrecht","year":"1997","journal-title":"Antonie van Leeuwenhoek"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB46","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1007\/BF00872193","article-title":"Anaerobic digestion and wastewater treatment systems","volume":"67","author":"Lettinga","year":"1995","journal-title":"Antonie van Leeuwenhoek"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB47","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1007\/BF00871644","article-title":"Metabolic interactions between anaerobic bacteria in methanogenic environments","volume":"66","author":"Stams","year":"1994","journal-title":"Antonie van Leeuwenhoek"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB48_1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/BF00360919","article-title":"Anaerobic bioprocessing of organic wastes","volume":"12","author":"Verstraete","year":"1996","journal-title":"World J Microbiol Biotechnol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB49","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/BF00872194","article-title":"Anaerobic treatment of sulphate-containing waste streams","volume":"67","author":"Colleran","year":"1995","journal-title":"Antonie van Leeuwenhoek"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB50","doi-asserted-by":"crossref","first-page":"2239","DOI":"10.1016\/0043-1354(95)00238-3","article-title":"Interaction between acetate fed sulfate reducers and methanogens","volume":"30","author":"Bhattacharya","year":"1996","journal-title":"Wat Res"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB51","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.2175\/106143096X128126","article-title":"Kinetics of growth, substrate utilization and sulfide toxicity for propionate, acetate, and hydrogen utilizers in anaerobic systems","volume":"68","author":"Maillacheruvu","year":"1996","journal-title":"Wat Environ Res"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB52","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1111\/j.1574-6976.1994.tb00130.x","article-title":"Sulfate reduction in methanogenic bioreactors","volume":"15","author":"Oude Elferink","year":"1994","journal-title":"FEMS Microbiol Rev"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB53","doi-asserted-by":"crossref","DOI":"10.1046\/j.1365-2672.1997.00197.x","article-title":"Detection and quantification of Desulforhabdus amnigenus in anaerobic granular sludge by dot-blot hybridization and PCR amplification","author":"Oude Elferink","year":"1997","journal-title":"J Appl Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB54_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/BF00872195","article-title":"Enhanced biodegradation of aromatic pollutants in cocultures of anaerobic and aerobic bacterial consortia","volume":"67","author":"Field","year":"1995","journal-title":"Antonie van Leeuwenhoek"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB55","doi-asserted-by":"crossref","first-page":"1919","DOI":"10.1128\/AEM.56.6.1919-1925.1990","article-title":"Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations","volume":"56","author":"Amann","year":"1990","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB56","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/S0723-2020(11)80121-9","article-title":"Phylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria: problems and solutions","volume":"15","author":"Manz","year":"1992","journal-title":"Syst Appl Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB57","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/S0723-2020(11)80122-0","article-title":"Genus- and group specific hybridization probes for determinative and environmental studies of sulfate-reducing bacteria","volume":"15","author":"Devereux","year":"1992","journal-title":"Syst Appl Microbiol"},{"key":"10.1016\/S0958-1669(97)80012-2_BIB58","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S0723-2020(11)80450-9","article-title":"Phylogenetic analysis of two syntrophic propionate-oxidizing bacteria in enrichment cultures","volume":"18","author":"Harmsen","year":"1995","journal-title":"Syst Appl Microbiol"}],"container-title":["Current Opinion in Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0958166997800122?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0958166997800122?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T12:28:30Z","timestamp":1620131310000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0958166997800122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,6]]},"references-count":58,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,6]]}},"alternative-id":["S0958166997800122"],"URL":"https:\/\/doi.org\/10.1016\/s0958-1669(97)80012-2","relation":{},"ISSN":["0958-1669"],"issn-type":[{"value":"0958-1669","type":"print"}],"subject":[],"published":{"date-parts":[[1997,6]]}}}