{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T23:28:40Z","timestamp":1772753320219,"version":"3.50.1"},"reference-count":52,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[1999,4,1]],"date-time":"1999-04-01T00:00:00Z","timestamp":922924800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mutation Research\/Genetic Toxicology and Environmental Mutagenesis"],"published-print":{"date-parts":[[1999,4]]},"DOI":"10.1016\/s1383-5718(99)00032-7","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T18:53:50Z","timestamp":1027623230000},"page":"73-83","source":"Crossref","is-referenced-by-count":28,"title":["Escherichia coli MTC, a human NADPH P450 reductase competent mutagenicity tester strain for the expression of human cytochrome P450 isoforms 1A1, 1A2, 2A6, 3A4, or 3A5: catalytic activities and mutagenicity studies"],"prefix":"10.1016","volume":"441","author":[{"given":"Michel","family":"Kranendonk","sequence":"first","affiliation":[]},{"given":"Filipa","family":"Carreira","sequence":"additional","affiliation":[]},{"given":"Patricia","family":"Theisen","sequence":"additional","affiliation":[]},{"given":"Antonio","family":"Laires","sequence":"additional","affiliation":[]},{"given":"Charles W","family":"Fisher","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9","family":"Rueff","sequence":"additional","affiliation":[]},{"given":"Ronald W","family":"Estabrook","sequence":"additional","affiliation":[]},{"given":"Nico P.E","family":"Vermeulen","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"key":"10.1016\/S1383-5718(99)00032-7_BIB1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0163-7258(92)90050-A","article-title":"Metabolic activation of carcinogens","volume":"54","author":"Guengerich","year":"1992","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB2","doi-asserted-by":"crossref","first-page":"165","DOI":"10.3109\/03602539409029789","article-title":"Role of human cytochrome P450 in the metabolic activation of chemical carcinogens and toxins","volume":"26","author":"Gonzalez","year":"1994","journal-title":"Drug Chem. Rev."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB3","doi-asserted-by":"crossref","first-page":"2327","DOI":"10.1002\/1097-0142(19810515)47:10<2327::AID-CNCR2820471003>3.0.CO;2-Z","article-title":"Searches for ultimate carcinogens and their reaction with cellular macromolecules","volume":"47","author":"Miller","year":"1981","journal-title":"Cancer"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB4","unstructured":"N.P.E. Vermeulen, Role of metabolism in chemical toxicity, in: C. Ioannides (Ed.), Cytochrome P450: Metabolic and Toxicological Aspects, CRC Press, Boca Raton, FL, 1996, pp. 29\u201353."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB5","doi-asserted-by":"crossref","unstructured":"D. Anderson, Genotoxicity assays, in: E. Arin\u00e7, J.B. Schenkman, E. Hodgson (Eds.), Molecular Aspects of Oxidative Drug Metabolizing Enzymes, NATO ASI Series, Vol. H90, Springer-Verlag, Berlin, Germany, 1995, pp. 303\u2013396.","DOI":"10.1007\/978-3-642-79528-2_16"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB6","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1146\/annurev.pa.35.040195.002101","article-title":"Cytochromes P450 expression systems","volume":"35","author":"Gonzalez","year":"1994","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB7","doi-asserted-by":"crossref","unstructured":"F.P. Guengerich, Enzymology of rat liver cytochrome P450, in: F.P. Guengerich, (Ed.), Mammalian Cytochromes P450, Vol. 1, CRC Press, Boca Raton, FL, 1987, pp. 1\u201354.","DOI":"10.1016\/B978-0-12-801238-3.01960-7"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB8","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1021\/tx00022a001","article-title":"Oxidation of toxic and carcinogenic chemicals by human cytochrome P450","volume":"4","author":"Guengerich","year":"1991","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB9","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/0165-6147(92)90107-H","article-title":"Human cytochrome P450: problems and prospects","volume":"13","author":"Gonzalez","year":"1992","journal-title":"Trends Pharmacol. Sci."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB10","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3109\/10408449509089886","article-title":"Oxygen and xenobiotic reductase activities of cytochrome P450","volume":"25","author":"Goeptar","year":"1995","journal-title":"Crit. Rev. Toxicol."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB11","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1080\/10408449991349186","article-title":"Genetic polymorphisms of human N-acetyltransferase, cytochrome P450, glutathione S-transferase and epoxide hydrolase enzymes","volume":"29","author":"Wormhoudt","year":"1999","journal-title":"Crit. Rev. Toxicol."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB12","unstructured":"J.W. Bridges, S.A. Hubbard, Problems in providing an appropriate drug metabolising system for short-term tests for carcinogens, in: G.M. Wialliams, R. Kroes, H.W. Waaijers, K.W. van de Poll (Eds.), The Predictive Value of Short-term Screening Tests in Carcinogenicity Evaluation, Elsevier, Amsterdam, 1980, pp. 69\u201388."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB13","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/0165-1110(92)90047-D","article-title":"Recombinant DNA approaches for the development of metabolic systems used in in vitro toxicology","volume":"277","author":"Langenbach","year":"1992","journal-title":"Mutat. Res."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB14","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/0027-5107(95)00246-4","article-title":"Development and validation of alternative metabolic systems for mutagenicity testing in short-term assays","volume":"353","author":"Rueff","year":"1996","journal-title":"Mutat. Res."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB15","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0027-5107(91)90039-Q","article-title":"cDNA-expressed human cytochrome P450s: a new age of molecular toxicology and human risk assessment","volume":"247","author":"Gonzalez","year":"1991","journal-title":"Mutat. Res."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB16","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1093\/mutage\/13.3.263","article-title":"Expression of human cytochrome P450 1A2 in Escherichia coli: a system for biotransformation and genotoxicity studies of chemical carcinogens","volume":"13","author":"Kranendonk","year":"1998","journal-title":"Mutagenesis"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB17","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/0165-1161(94)90014-0","article-title":"Isolation and prevalidation of an Escherichia coli tester strain for the use in mechanistic and metabolic studies of genotoxins","volume":"312","author":"Kranendonk","year":"1994","journal-title":"Mutat. Res."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB18","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1093\/mutage\/11.4.327","article-title":"MX100, a new Escherichia coli tester strain for use in genotoxicity studies","volume":"11","author":"Kranendonk","year":"1996","journal-title":"Mutagenesis"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB19","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1093\/mutage\/12.4.245","article-title":"Characterisation of enzyme activities and cofactors involved in bioactivation and bioinactivation of chemical carcinogens in the tester strains Escherichia coli K12 MX100 and Salmonella typhimurium LT2 TA100","volume":"12","author":"Kranendonk","year":"1997","journal-title":"Mutagenesis"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB20","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/S1383-5718(98)00193-4","article-title":"Escherichia coli MTC, a NADPH cytochrome P450 reductase competent mutagenicity tester strain for the expression of human cytochrome P450: comparison of three types of expression systems","volume":"439","author":"Kranendonk","year":"1999","journal-title":"Mutat. Res."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB21","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1089\/dna.1987.6.189","article-title":"A genetically engineered P450 monooxygenase: construction of the functional fused enzyme between rat cytochrome P450c and NADPH-cytochrome P450 reductase","volume":"6","author":"Murakami","year":"1987","journal-title":"DNA"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB22","doi-asserted-by":"crossref","first-page":"117448","DOI":"10.1073\/pnas.90.24.11748","article-title":"Human cytochrome P450 3A4: enzymatic properties of a purified recombinant fusion protein containing NADPH-P450 reductase","volume":"90","author":"Shet","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB23","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1038\/nbt0897-784","article-title":"Drug metabolism by Escherichia coli expressing human cytochrome P450","volume":"15","author":"Parikh","year":"1997","journal-title":"Nature Biotechnol."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB24","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/S0014-5793(96)01196-9","article-title":"Coexpression of a human P450 (CYP3A4) and P450 reductase generates a highly functional monooxygenase system in Escherichia coli","volume":"397","author":"Blake","year":"1996","journal-title":"FEBS Lett."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB25","first-page":"799","article-title":"Bioactivation of aromatic amines by recombinant human cytochrome P4501A2 expressed in Ames tester strain bacteria: a substitute for activation by mammalian tissue preparations","volume":"55","author":"Josephy","year":"1995","journal-title":"Cancer Res."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB26","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1021\/tx970171q","article-title":"Metabolic activation of aromatic amine mutagens by simultaneous expression of human cytochrome P450 1A2, NADPH cytochrome P450 reductase and N-acetyltransferase in Escherichia coli","volume":"11","author":"Josephy","year":"1998","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB27","first-page":"1833","article-title":"Establishment of a Salmonella tester strain highly sensitive to mutagenic heterocylic amines","volume":"58","author":"Suzuki","year":"1998","journal-title":"Cancer Res."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB28","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1096\/fasebj.6.2.1537466","article-title":"High level expression of functional human cytochrome P450 1A2 in Escherichia coli","volume":"6","author":"Fisher","year":"1992","journal-title":"FASEB J."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB29","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1093\/carcin\/17.9.1989","article-title":"Cytochrome P450 species involved in the metabolism of quinoline","volume":"17","author":"Reigh","year":"1996","journal-title":"Carcinogenesis"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB30","first-page":"502","article-title":"Human cytochrome P450 3A4-catalysed testosterone 6\u03b2-hydroxylation and erythromycin N-demethylation. Competition during catalysis","volume":"25","author":"Wang","year":"1997","journal-title":"Drug Metab. Dispos."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB31","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1007\/BF02353478","article-title":"In vitro techniques for studying drug metabolism","volume":"24","author":"Guengerich","year":"1996","journal-title":"J. Pharmacokin. Biopharm."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB32","doi-asserted-by":"crossref","unstructured":"F.P. Guengerich, Human cytochrome P450 enzymes, in: P.R. Ortiz de Montellano (Ed.), Cytochrome P450, Plenum, New York, 1995, pp. 473\u2013535.","DOI":"10.1007\/978-1-4757-2391-5_14"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB33","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/S0958-1669(97)80039-0","article-title":"Expression of drug-metabolising enzymes","volume":"8","author":"Guengerich","year":"1997","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB34","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1006\/prep.1996.0122","article-title":"The use of macroporous microcarriers for the large-scale growth of V79 cells genetically designed to express single human cytochrome P450 isoenzymes and for the characterisation of the expressed cytochrome P450","volume":"8","author":"Onderwater","year":"1996","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB35","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1093\/carcin\/15.9.1931","article-title":"Development of a human lymphoblastoid cell line constitutively expressing human CYP1A1 cDNA: substrate specificity with model substrates and promutagens","volume":"15","author":"Penman","year":"1994","journal-title":"Carcinogenesis"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB36","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1006\/abbi.1995.1177","article-title":"Expression of cytochrome P450 3A5 in Escherichia coli: effects of 5\u2032 modification, purification, spectral characterisation, reconstitution conditions and catalytic activities","volume":"317","author":"Gillam","year":"1995","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB37","first-page":"207","article-title":"Studies on the expression and metabolic capabilities of human liver cytochrome P450IIIA5 (HLP3)","volume":"38","author":"Wrighton","year":"1990","journal-title":"Mol. Pharamacol."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB38","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1093\/carcin\/15.5.837","article-title":"High promutagen activating capacity of yeast microsomes containing human cytochrome P450 1A and human NADPH cytochrome P450 reductase","volume":"15","author":"Sengstag","year":"1994","journal-title":"Carcinogenesis"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB39","doi-asserted-by":"crossref","first-page":"4790","DOI":"10.1073\/pnas.87.12.4790","article-title":"Five of 12 forms of vaccinia virus-expressed human hepatic cytochrome P450 metabolically activate aflatoxin B1","volume":"87","author":"Aoyama","year":"1990","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB40","doi-asserted-by":"crossref","first-page":"8306","DOI":"10.1073\/pnas.87.21.8306","article-title":"Evidence for involvement of multiple forms of cytochrome P450 in aflataoxin B1 metabolism in human liver","volume":"87","author":"Forrester","year":"1990","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB41","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1093\/carcin\/12.2.355","article-title":"The development of a human cell line stably expressing human CYP3A4: role in the metabolic activation of aflatoxin B1 and comparison to CYP1A2 and CYP2A3","volume":"12","author":"Crespi","year":"1991","journal-title":"Carcinogenesis"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB42","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1146\/annurev.pa.34.040194.001031","article-title":"Mechanisms of aflatoxin carcinogenesis","volume":"34","author":"Eaton","year":"1994","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB43","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1093\/carcin\/16.9.2167","article-title":"Procarcinogen activation by cytochrome P450 3A4 and 3A5 expressed in Escherichia coli and by human liver microsomes","volume":"16","author":"Yamazaki","year":"1995","journal-title":"Carcinogenesis"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB44","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1073\/pnas.86.2.462","article-title":"Evidence for cytochrome P450NF, the nifedipine oxidase, being the principle enzyme involved in the bioactivation of aflatoxins in human liver","volume":"86","author":"Shimada","year":"1989","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/S1383-5718(99)00032-7_BIB45","first-page":"617","article-title":"Oxidations of xenobiotics by recombinant human cytochrome P450 1B1","volume":"29","author":"Shimada","year":"1997","journal-title":"Drug Metab. Dispos."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB46","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1021\/tx00044a006","article-title":"Oxidation of aflatoxin B1 by bacterial recombinant human cytochrome P450 enzymes","volume":"8","author":"Ueng","year":"1995","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB47","first-page":"101","article-title":"Role of human microsomal and human complementary DNA-expressed cytochrome P4501A2 and P4503A4 in the bioactivation of aflatoxin B1","volume":"54","author":"Gallagher","year":"1994","journal-title":"Cancer Res."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB48","first-page":"615","article-title":"Species susceptibility to aflatoxin B1 carcinogenesis: comparative kinetics of microsomal biotransformation","volume":"50","author":"Ramsdell","year":"1990","journal-title":"Cancer Res."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB49","first-page":"154","article-title":"Cytochrome P450 inhibitors","volume":"23","author":"Newton","year":"1995","journal-title":"Drug Metab. Dispos."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB50","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1006\/abbi.1994.1259","article-title":"Evaluation of triacetyloleandomycin, \u03b1-naphtoflavone and diethyldithiocarbamate as selective chemical probes for the inhibition of human cytochrome P450","volume":"311","author":"Chang","year":"1994","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB51","first-page":"401","article-title":"Specificity of substrate and inhibitor probes for human cytochrome P450 1A1 and 1A2","volume":"265","author":"Tassaneeyakul","year":"1993","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/S1383-5718(99)00032-7_BIB52","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1016\/S0006-2952(97)00643-6","article-title":"High catalytic activity of human cytochrome P450 coexpressed with human NADPH-cytochrome P450 reductase in Escherichia coli","volume":"55","author":"Iwata","year":"1998","journal-title":"Biochem. Pharmacol."}],"container-title":["Mutation Research\/Genetic Toxicology and Environmental Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1383571899000327?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1383571899000327?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,1,28]],"date-time":"2020-01-28T19:31:40Z","timestamp":1580239900000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1383571899000327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,4]]},"references-count":52,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1999,4]]}},"alternative-id":["S1383571899000327"],"URL":"https:\/\/doi.org\/10.1016\/s1383-5718(99)00032-7","relation":{},"ISSN":["1383-5718"],"issn-type":[{"value":"1383-5718","type":"print"}],"subject":[],"published":{"date-parts":[[1999,4]]}}}