{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T07:52:50Z","timestamp":1777708370572,"version":"3.51.4"},"reference-count":5,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","em-consulte.com","thelancet.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Lancet Infectious Diseases"],"published-print":{"date-parts":[[2021,2]]},"DOI":"10.1016\/s1473-3099(20)30435-7","type":"journal-article","created":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T18:51:44Z","timestamp":1590691904000},"page":"166-167","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":41,"title":["Implication of SARS-CoV-2 evolution in the sensitivity of RT-qPCR diagnostic assays"],"prefix":"10.1016","volume":"21","author":[{"given":"Nuno Sampaio","family":"Os\u00f3rio","sequence":"first","affiliation":[]},{"given":"Margarida","family":"Correia-Neves","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"key":"10.1016\/S1473-3099(20)30435-7_bib1","article-title":"Early phylogenetic estimate of the effective reproduction number of SARS-CoV-2","author":"Lai","year":"2020","journal-title":"J Med Virol"},{"key":"10.1016\/S1473-3099(20)30435-7_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.meegid.2020.104260","article-title":"Genetic diversity and evolution of SARS-CoV-2","volume":"81","author":"Phan","year":"2020","journal-title":"Infect Genet Evol"},{"key":"10.1016\/S1473-3099(20)30435-7_bib3","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1002\/jmv.25701","article-title":"Transmission dynamics and evolutionary history of 2019-nCoV","volume":"92","author":"Li","year":"2020","journal-title":"J Med Virol"},{"key":"10.1016\/S1473-3099(20)30435-7_bib4","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2017.22.13.30494","article-title":"GISAID: Global Initiative on Sharing All Influenza Data\u2014from vision to reality","volume":"22","author":"Shu","year":"2017","journal-title":"Euro Surveill"},{"key":"10.1016\/S1473-3099(20)30435-7_bib6","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1093\/genetics\/123.3.585","article-title":"Statistical methods to test for nucleotide mutation hypothesis by DNA polymorphism","volume":"123","author":"Tajima","year":"1989","journal-title":"Genetics"}],"container-title":["The Lancet Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1473309920304357?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1473309920304357?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T09:30:58Z","timestamp":1761471058000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1473309920304357"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2]]},"references-count":5,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["S1473309920304357"],"URL":"https:\/\/doi.org\/10.1016\/s1473-3099(20)30435-7","relation":{},"ISSN":["1473-3099"],"issn-type":[{"value":"1473-3099","type":"print"}],"subject":[],"published":{"date-parts":[[2021,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Implication of SARS-CoV-2 evolution in the sensitivity of RT-qPCR diagnostic assays","name":"articletitle","label":"Article Title"},{"value":"The Lancet Infectious Diseases","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/S1473-3099(20)30435-7","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"simple-article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}