{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T11:54:34Z","timestamp":1776945274121,"version":"3.51.4"},"reference-count":24,"publisher":"Hindawi Limited","issue":"1","license":[{"start":{"date-parts":[[2009,4,14]],"date-time":"2009-04-14T00:00:00Z","timestamp":1239667200000},"content-version":"unspecified","delay-in-days":14136,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genet. Res."],"published-print":{"date-parts":[[1970,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>We have recently described a chain-forming mutant of<jats:italic>Escherichia coli<\/jats:italic>which showed a decreased resistance to ampicillin and several other antibiotics (Normark, Boman &amp; Mattson, 1969). The gene mediating drug sensitivity was denoted<jats:italic>envA<\/jats:italic>and by conjugation mapped at 2\u20134 min. Transduction experiments have now shown that<jats:italic>envA<\/jats:italic>is located between<jats:italic>leu<\/jats:italic>and<jats:italic>azi<\/jats:italic>at 1\u00b75 min. The mapping was facilitated by the finding that<jats:italic>envA<\/jats:italic>mediated sensitivity to actinomycin D, rifampicin and gentian violet. The<jats:italic>envA<\/jats:italic>locus could be genetically differentiated from the<jats:italic>pea<\/jats:italic>locus mediating resistance to phenethyl alcohol (Yura &amp; Wada, 1968). Studies using partial diploids revealed that<jats:italic>envA<\/jats:italic>was recessive to its wild-type allele both when located on an episome and on the chromosome.<\/jats:p><jats:p>Assuming that revertants from<jats:italic>envA<\/jats:italic>to the wild-type allele could be selected as ampicillin-resistant derivatives, such mutants were isolated and their phenotype characterized. Reversion to ampicillin resistance was accompanied by reversion to insensitivity to actinomycin D. However, not all revertants exhibited wild-type tolerance to rifampicin. Three different ampicillin-resistant revertants were studied genetically. The results indicate that these strains contain suppressor mutations in the<jats:italic>envA<\/jats:italic>region of the chromosome. It is suggested that the<jats:italic>envA<\/jats:italic>gene, directly or indirectly, affects the EDTA sensitive \u2018permeability barrier\u2019 of the surface layer of<jats:italic>Escherichia coli<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0016672300002287","type":"journal-article","created":{"date-parts":[[2009,9,17]],"date-time":"2009-09-17T07:53:19Z","timestamp":1253173999000},"page":"63-78","source":"Crossref","is-referenced-by-count":64,"title":["Genetics of a chain-forming mutant of<i>Escherichia coli<\/i>: Transduction and dominance of the<i>envA<\/i>gene mediating increased penetration to some antibacterial agents"],"prefix":"10.1155","volume":"16","author":[{"given":"Staffan","family":"Normark","sequence":"first","affiliation":[]}],"member":"98","published-online":{"date-parts":[[2009,4,14]]},"reference":[{"key":"S0016672300002287_ref008","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1128\/JB.96.4.975-980.1968","article-title":"Selection of a mutant of Escherichia coli which has high mutation rates","volume":"96","author":"Helling","year":"1968","journal-title":"Journal of Bacteriology"},{"key":"S0016672300002287_ref021","doi-asserted-by":"publisher","DOI":"10.1038\/220275a0"},{"key":"S0016672300002287_ref015","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300011770"},{"key":"S0016672300002287_ref007","volume-title":"The Genetics of Bacteria and Their Viruses","author":"Hayes","year":"1968"},{"key":"S0016672300002287_ref010","doi-asserted-by":"crossref","first-page":"6384","DOI":"10.1016\/S0021-9258(18)93151-0","article-title":"Physical, chemical and immunological properties of lipopolysaccharide released from Escherichia coli by ethylenediamine-tetraacetate","volume":"243","author":"Leive","year":"1968","journal-title":"Journal of Biological Chemistry"},{"key":"S0016672300002287_ref003","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.53.2.451"},{"key":"S0016672300002287_ref001","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1128\/JB.62.3.293-300.1951","article-title":"Studies on lysogenesis. I. The mode of phage-liberation by lysogenic Escherichia coli","volume":"62","author":"Bertani","year":"1951","journal-title":"Journal of Bacteriology"},{"key":"S0016672300002287_ref009","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.53.4.745"},{"key":"S0016672300002287_ref012","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(60)90177-7"},{"key":"S0016672300002287_ref002","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300011782"},{"key":"S0016672300002287_ref020","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1128\/MMBR.31.4.332-353.1967","article-title":"Revised linkage map of Escherichia coli","volume":"31","author":"Taylor","year":"1967","journal-title":"Bacteriological Reviews"},{"key":"S0016672300002287_ref017","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.58.6.2315"},{"key":"S0016672300002287_ref023","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0021-9258(18)65874-0","article-title":"Acetylornithinase of Escherichia coli: partial purification and some properties","volume":"218","author":"Vogel","year":"1956","journal-title":"Journal of Biological Chemistry"},{"key":"S0016672300002287_ref022","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(64)90014-4"},{"key":"S0016672300002287_ref013","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.54.4.1084"},{"key":"S0016672300002287_ref006","doi-asserted-by":"publisher","DOI":"10.1038\/220276a0"},{"key":"S0016672300002287_ref014","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300009538"},{"key":"S0016672300002287_ref004","volume-title":"Experiments in Microbial Genetics","author":"Clowes","year":"1968"},{"key":"S0016672300002287_ref016","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1128\/JB.97.3.1334-1342.1969","article-title":"A mutant of Escherichia coli K 12 with anomalous cell division and ability to decrease episomally and chromosomally mediated resistance to ampicillin and several other antibiotics","volume":"97","author":"Normark","year":"1969","journal-title":"Journal of Bacteriology"},{"key":"S0016672300002287_ref018","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(65)80237-6"},{"key":"S0016672300002287_ref019","doi-asserted-by":"publisher","DOI":"10.1038\/2231111a0"},{"key":"S0016672300002287_ref024","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1093\/genetics\/59.2.177","article-title":"Phenethyl alcohol resistance in Escherichia coli. 1. Resistance of strain C 600 and its relation to azide resistance","volume":"59","author":"Yura","year":"1968","journal-title":"Genetics"},{"key":"S0016672300002287_ref005","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300011769"},{"key":"S0016672300002287_ref011","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.60.1.160"}],"container-title":["Genetical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0016672300002287","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,10]],"date-time":"2021-10-10T22:52:24Z","timestamp":1633906344000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0016672300002287\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,8]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1970,8]]}},"alternative-id":["S0016672300002287"],"URL":"https:\/\/doi.org\/10.1017\/s0016672300002287","relation":{},"ISSN":["0016-6723","1469-5073"],"issn-type":[{"value":"0016-6723","type":"print"},{"value":"1469-5073","type":"electronic"}],"subject":[],"published":{"date-parts":[[1970,8]]}}}