{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T18:53:03Z","timestamp":1699642383651},"reference-count":27,"publisher":"Hindawi Limited","issue":"2","license":[{"start":{"date-parts":[[2009,4,14]],"date-time":"2009-04-14T00:00:00Z","timestamp":1239667200000},"content-version":"unspecified","delay-in-days":14805,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genet. Res."],"published-print":{"date-parts":[[1968,10]]},"abstract":"<jats:p>The first two steps towards increasing ampicillin resistance in<jats:italic>Escherichia coli<\/jats:italic>concern the genes<jats:italic>ampA<\/jats:italic>and<jats:italic>ampB<\/jats:italic>which are located at least 20 min from each other (Eriksson-Grennberg<jats:italic>et al.<\/jats:italic>1965 and the two preceding papers). This paper describes a third class of<jats:italic>ampA<\/jats:italic>-containing mutants (designated<jats:italic>ampAB<\/jats:italic>) which are resistant to D, L-ampicillin concentrations of 100 \u03bcg\/ml. When such as F<jats:sup>-<\/jats:sup>strain (D31) was crossed to different Hfr strains analysis of the<jats:italic>trp<\/jats:italic><jats:sup>+<\/jats:sup>and<jats:italic>proB<\/jats:italic><jats:sup>+<\/jats:sup>recombinants indicated that resistance genes were located between<jats:italic>trp<\/jats:italic>and<jats:italic>proB<\/jats:italic>. Altogether five classes of recombinants were produced but only the two genes<jats:italic>ampA<\/jats:italic>and<jats:italic>ampB<\/jats:italic>were recovered. One interpretation suggested is that the resistance of D31 is due to the presence of<jats:italic>ampA<\/jats:italic>and<jats:italic>ampB<\/jats:italic>and a chromosomal aberration by which<jats:italic>ampA<\/jats:italic>was moved to a position near<jats:italic>ampB<\/jats:italic>. It was possible to transduce both intermediate levels of resistance as well as the full resistance of an<jats:italic>ampAB<\/jats:italic>donor strain, but the strains produced were unstable. In crosses the presence of<jats:italic>ampAB<\/jats:italic>in the recipient reduced the number of recombinants by reducing the number of stable pairs. In an Hfr strain<jats:italic>ampAB<\/jats:italic>was shown to give rise to additional difficulties in establishing the cell contact during mating. Some ampicillin resistant mutants also showed decreased ability to propagate the RNA phage MS2.<\/jats:p>","DOI":"10.1017\/s0016672300011782","type":"journal-article","created":{"date-parts":[[2009,9,17]],"date-time":"2009-09-17T07:55:17Z","timestamp":1253174117000},"page":"169-185","source":"Crossref","is-referenced-by-count":50,"title":["Resistance of<i>Escherichia coli<\/i>to penicillins: IV. Genetic study of mutants resistant to D, L-ampicillin concentrations of 100 \u03bcg\/ml."],"prefix":"10.1155","volume":"12","author":[{"given":"Hans G.","family":"Boman","sequence":"first","affiliation":[]},{"given":"Kerstin G.","family":"Eriksson-Grennberg","sequence":"additional","affiliation":[]},{"given":"Staffan","family":"Normark","sequence":"additional","affiliation":[]},{"given":"Eva","family":"Matsson","sequence":"additional","affiliation":[]}],"member":"98","published-online":{"date-parts":[[2009,4,14]]},"reference":[{"key":"S0016672300011782_ref026","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1128\/br.27.1.87-115.1963","article-title":"Infective heredity of multiple drug resistance in bacteria","volume":"27","author":"Watanabe","year":"1963","journal-title":"Bact. Rev."},{"key":"S0016672300011782_ref025","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1093\/genetics\/50.4.659","article-title":"The genetic map of Escherichia coli K-12","volume":"50","author":"Taylor","year":"1964","journal-title":"Genetics"},{"key":"S0016672300011782_ref022","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.47.12.2005"},{"key":"S0016672300011782_ref021","doi-asserted-by":"crossref","first-page":"2071","DOI":"10.1128\/JB.94.6.2071-2072.1967","article-title":"R-factor infection of Escherichia coli lyophilized in 1946","volume":"94","author":"Smith","year":"1967","journal-title":"J. Bact."},{"key":"S0016672300011782_ref020","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300011228"},{"key":"S0016672300011782_ref019","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300011484"},{"key":"S0016672300011782_ref018","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.4.5571.71"},{"key":"S0016672300011782_ref017","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-50-1-159"},{"key":"S0016672300011782_ref016","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300011770"},{"key":"S0016672300011782_ref015","unstructured":"Nordstr\u00f6m K. & Burman L. G. (1968). Resistance of Escherichia coli to penicillins. VI. AmpB mediated sensitivity to osmotic shock and to cycloserine. To be published."},{"key":"S0016672300011782_ref014","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300009538"},{"key":"S0016672300011782_ref013","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1128\/JB.94.5.1497-1501.1967","article-title":"Derepression of phospho-mannose isomerase by regulator gene mutations involved in capsular polysaccharide synthesis in Escherichia coli K-12","volume":"94","author":"Markovitz","year":"1967","journal-title":"J. Bact."},{"key":"S0016672300011782_ref011","volume-title":"The Genetics of Bacteria and their Viruses","author":"Hayes","year":"1964"},{"key":"S0016672300011782_ref010","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1128\/JB.93.4.1236-1241.1967","article-title":"Drug resistance of enteric bacteria. VIII. Chromosomal location of nontransferable R factor in Escherichia coli","volume":"93","author":"Harada","year":"1967","journal-title":"J. Bact."},{"key":"S0016672300011782_ref009","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300035047"},{"key":"S0016672300011782_ref007","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300011769"},{"key":"S0016672300011782_ref008","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1128\/JB.90.1.54-62.1965","article-title":"Resistance of Escherichia coli to penicillins. I. Genetic study of some ampicillin-resistant mutants","volume":"90","author":"Eriksson-Grennberg","year":"1965","journal-title":"J. Bact."},{"key":"S0016672300011782_ref023","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1093\/genetics\/45.9.1233","article-title":"Linkage analysis with very high frequency males of Escherichia coli","volume":"45","author":"Taylor","year":"1960","journal-title":"Genetics"},{"key":"S0016672300011782_ref004","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300010296"},{"key":"S0016672300011782_ref012","first-page":"43","article-title":"Purification of penicillinase from wild type and two ampicillin resistant mutants of Escherichia coli","volume":"106","author":"Lindstr\u00f6m","year":"1968","journal-title":"Biochem. J."},{"key":"S0016672300011782_ref006","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1128\/JB.89.1.28-40.1965","article-title":"Chromosomal aberrations associated with mutations to bacteriophage resistance in Escherichia coli","volume":"89","author":"Curtiss","year":"1965","journal-title":"J. Bact."},{"key":"S0016672300011782_ref027","first-page":"23","article-title":"Evolutionary relationships of R factors with other episomes and plasmids","volume":"26","author":"Watanabe","year":"1967","journal-title":"Fed. Proc."},{"key":"S0016672300011782_ref001","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-31-3-339"},{"key":"S0016672300011782_ref003","first-page":"37","volume-title":"The Specificity of Cell Surfaces","author":"Brinton","year":"1967"},{"key":"S0016672300011782_ref024","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1128\/br.31.4.332-353.1967","article-title":"Revised linkage map of Escherichia coli","volume":"31","author":"Taylor","year":"1967","journal-title":"Bact. Rev."},{"key":"S0016672300011782_ref002","first-page":"366","volume-title":"Regulation of Nucleic Acid and Protein Biosynthesis","author":"Boman","year":"1967"},{"key":"S0016672300011782_ref005","article-title":"Resistance of Escherichia coli to penicillins. V. Physiological comparison of two isogenic strains, one with chromosomally and one with episomally mediated ampicillin resistance","volume":"96","author":"Burman","year":"1968","journal-title":"J. Bact."}],"container-title":["Genetical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0016672300011782","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,10]],"date-time":"2021-10-10T22:53:15Z","timestamp":1633906395000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0016672300011782\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,10]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1968,10]]}},"alternative-id":["S0016672300011782"],"URL":"https:\/\/doi.org\/10.1017\/s0016672300011782","relation":{},"ISSN":["0016-6723","1469-5073"],"issn-type":[{"value":"0016-6723","type":"print"},{"value":"1469-5073","type":"electronic"}],"subject":[],"published":{"date-parts":[[1968,10]]}}}