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Both epitopes contain fucose: mAb 128C3\/3, as we have shown previously, recognizes fucose in a novel, possibly internal linkage (Levery<jats:italic>et al.<\/jats:italic>1992) while mAb 504B1, as we show here, bound to the Le<jats:sup>x<\/jats:sup>epitope, which contains fucose \u03b11 \u2192 3 linked to<jats:italic>N<\/jats:italic>-acetyl-glucosamine. The tissue expression of these epitopes was strikingly different and both elicit an immune response in infected hosts. The mAb 128C3\/3-defined epitope was exposed on the surface of all larval stages but not on adult worms; however, it was found in the excretory system of adult worms of both sexes. In contrast, surface expression of the Le<jats:sup>x<\/jats:sup>epitope was initiated after the transformation of cercariae to schistosomula and was maintained throughout the adult life in both sexes.<\/jats:p>","DOI":"10.1017\/s0031182000075995","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:00:10Z","timestamp":1244210410000},"page":"433-446","source":"Crossref","is-referenced-by-count":48,"title":["<i>Schistosoma mansoni<\/i>: immunolocalization of two different fucose-containing carbohydrate epitopes"],"prefix":"10.1017","volume":"108","author":[{"given":"B.","family":"K\u00f6ster","sequence":"first","affiliation":[]},{"given":"M.","family":"Strand","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000075995_ref018","doi-asserted-by":"crossref","first-page":"998","DOI":"10.4049\/jimmunol.133.2.998","article-title":"Blocking activity of rat monoclonal antibodies in experimental schistosomiasis","volume":"133","author":"Grzych","year":"1984","journal-title":"Journal of Immunology"},{"key":"S0031182000075995_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(85)90012-X"},{"key":"S0031182000075995_ref061","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.4049\/jimmunol.139.5.1631","article-title":"Monoclonal antibodies that recognize lacto-N-fucopentaose III (CD15) react with the adhesion-promoting glycoprotein family (LFA-1\/HMAC-l\/GP 150, 95) and CR1 on human neutrophils","volume":"139","author":"Skubitz","year":"1987","journal-title":"Journal of Immunology"},{"key":"S0031182000075995_ref067","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(82)90121-7"},{"key":"S0031182000075995_ref026","doi-asserted-by":"publisher","DOI":"10.2307\/3281844"},{"key":"S0031182000075995_ref062","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.11.5565"},{"key":"S0031182000075995_ref006","doi-asserted-by":"crossref","first-page":"4689","DOI":"10.4049\/jimmunol.136.12.4689","article-title":"Identification of Schistosoma mansoni glycoproteins recognized by protective antibodies from mice immunized with irradiated cercariae","volume":"136","author":"Dalton","year":"1986","journal-title":"Journal of Immunology"},{"key":"S0031182000075995_ref041","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1989.41.338"},{"key":"S0031182000075995_ref060","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000054986"},{"key":"S0031182000075995_ref034","unstructured":"K\u00f6ster B. & Strand M. (1989). Developmentally regulated expression of Schistosoma mansoni glycoproteins. The 38th Annual Meeting of the American Society for Tropical Medicine and Hygiene, Honolulu, Hawaii."},{"key":"S0031182000075995_ref032","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.11.4159"},{"key":"S0031182000075995_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(76)90081-3"},{"key":"S0031182000075995_ref023","unstructured":"Harn D. A. & Palanivel V. (1993). Oligosaccharide (Lewis+) stimulated B220+ cells produce IL-10 and may contribute to downregulation of Th1CD4+ T cells in murine schistosomiasis. SRP International Conference on Schistosomiasis, Cairo, Egypt."},{"key":"S0031182000075995_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(87)90164-0"},{"key":"S0031182000075995_ref063","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(69)90033-1"},{"key":"S0031182000075995_ref048","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/0035-9203(89)90503-8","article-title":"Antibody to schistosomulum surface carbohydrate epitopes in subjects infected with Schistosoma haematobium","volume":"83","author":"Omer-Ali","year":"1989","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"key":"S0031182000075995_ref012","doi-asserted-by":"publisher","DOI":"10.1038\/323443a0"},{"key":"S0031182000075995_ref033","unstructured":"K\u00f6ster B. (1987). Schistosoma mansoni: production of monoclonal antibodies and immunolocalization of their epitopes in different developmental stages. Ph.D. thesis. 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