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This study presents a modular and wearable-sensor-based experimental protocol designed to bridge this gap by enabling the evaluation of exoskeletons in both static (STC) and dynamic (DYN) tasks while preserving natural movement variability. A total of 52 participants, including both men and women, completed tasks with and without two different passive exoskeletons, while their motor activity was assessed using surface electromyography (sEMG) and inertial motion sensors. The protocol incorporates key EMG-based metrics \u2013 Root Mean Square (RMS) and Hilbert Median Frequency (MDF) \u2013 that effectively quantify muscle activation and fatigue, along with subjective Perceived Fatigue Scores (PFS) and a task performance metric (Screwing Velocity, SV). The results confirm that the exoskeletons significantly reduce muscle activation and perceived fatigue without impairing task performance. The proposed methodology, combining rigorous metrics with wearable and non-invasive instrumentation, offers a robust framework for evaluating fatigue in both STC and DYN tasks and usability in both laboratory and field settings. This protocol represents a valuable tool for both research and industrial evaluation, facilitating the evidence-based integration of exoskeletons into real-world industrial workflows.<\/jats:p>","DOI":"10.1017\/s0263574725103019","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T12:22:49Z","timestamp":1767010969000},"page":"471-498","source":"Crossref","is-referenced-by-count":0,"title":["Reducing muscle effort with upper-limb exoskeletons: an electromyography (EMG) and perceived fatigue assessment"],"prefix":"10.1017","volume":"44","author":[{"given":"Serenella","family":"Terlizzi","sequence":"first","affiliation":[{"name":"Universit\u00e0 Politecnica delle Marche"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuele","family":"Tonelli","sequence":"additional","affiliation":[{"name":"Universit\u00e0 Politecnica delle Marche"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianna","family":"Ciccarelli","sequence":"additional","affiliation":[{"name":"Universit\u00e0 Politecnica delle Marche"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Papetti","sequence":"additional","affiliation":[{"name":"Universit\u00e0 Politecnica delle Marche"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1763-2616","authenticated-orcid":false,"given":"Cecilia","family":"Scoccia","sequence":"additional","affiliation":[{"name":"Universit\u00e0 Politecnica delle Marche"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2025,12,29]]},"reference":[{"key":"S0263574725103019_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.apergo.2022.103903"},{"key":"S0263574725103019_ref17","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-60825-9_26"},{"key":"S0263574725103019_ref21","first-page":"81","volume-title":"European Robotics Forum, 2024b","author":"Scoccia"},{"key":"S0263574725103019_ref2","volume-title":"OSH in Figures: Occupational Safety and Health in the Transport Sector: An Overview","author":"Schneider","year":"2011"},{"key":"S0263574725103019_ref5","doi-asserted-by":"crossref","unstructured":"[5] Lauret, L. , Raiteri, B. 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